Salmonella typhimurium cobalamin (vitamin B12) biosynthetic genes: functional studies in S. typhimurium and Escherichia coli.

Abstract:

:In order to study the Salmonella typhimurium cobalamin biosynthetic pathway, the S. typhimurium cob operon was isolated and cloned into Escherichia coli. This approach has given the new host of the cob operon the ability to make cobalamins de novo, an ability that had probably been lost by this organism. In total, 20 genes of the S. typhimurium cob operon have been transferred into E. coli, and the resulting recombinant strains have been shown to produce up to 100 times more corrin than the parent S. typhimurium strain. These measurements have been performed with a quantitative cobalamin microbiological assay which is detailed in this work. As with S. typhimurium, cobalamin synthesis is only observed in the E. coli cobalamin-producing strains when they are grown under anaerobic conditions. Derivatives of the cobalamin-producing E. coli strains were constructed in which genes of the cob operon were inactivated. These strains, together with S. typhimurium cob mutants, have permitted the determination of the genes necessary for cobalamin production and classification of cbiD and cbiG as cobl genes. When grown in the absence of endogenous cobalt, the oxidized forms of precorrin-2 and precorrin-3, factor II and factor III, respectively, were found to accumulate in the cytosol of the corrin-producing E. coli. Together with the finding that S. typhimurium cbiL mutants are not complemented with the homologous Pseudomonas denitrificans gene, these results lend further credence to the theory that cobalt is required at an early stage in the biosynthesis of cobalamins in S. typhimurium.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Raux E,Lanois A,Levillayer F,Warren MJ,Brody E,Rambach A,Thermes C

doi

10.1128/jb.178.3.753-767.1996

subject

Has Abstract

pub_date

1996-02-01 00:00:00

pages

753-67

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

178

pub_type

杂志文章
  • Generation of auxotrophic mutants of Enterococcus faecalis.

    abstract::A 22-kb segment of chromosomal DNA from Enterococcus faecalis OG1RF containing the pyrimidine biosynthesis genes pyrC and pyrD was previously detected as complementing Escherichia coli pyrC and pyrD mutations. In the present study, it was found that the E. faecalis pyrimidine biosynthetic genes in this clone (designat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.23.6866-6873.1995

    authors: Li X,Weinstock GM,Murray BE

    更新日期:1995-12-01 00:00:00

  • Draft genome sequence of Bizionia argentinensis, isolated from Antarctic surface water.

    abstract::A psychrotolerant marine bacterial strain, designated JUB59(T), was isolated from Antarctic surface seawater and classified as a new species of the genus Bizionia. Here, we present the first draft genome sequence for this genus, which suggests interesting features such as UV resistance, hydrolytic exoenzymes, and nitr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.06245-11

    authors: Lanzarotti E,Pellizza L,Bercovich A,Foti M,Coria SH,Vazquez SC,Ruberto L,Hernández EA,Dias RL,Mac Cormack WP,Cicero DO,Smal C,Nicolas MF,Vasconcelos AT,Marti MA,Turjanski AG

    更新日期:2011-12-01 00:00:00

  • Further electron microscope characterization of spore appendages of Clostridium bifermentans.

    abstract::Two distinct spore appendage types of Clostridium bifermentans, a pinlike appendage and a tubular appendage, were studied by electron microscopy. The pinlike appendage is characterized by a shaft, about 100 A in diameter, which has a lobed caplike structure. The tubular appendage, 500 to 600 A in diameter, is characte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.1.231-238.1968

    authors: Yolton DP,Pope L,Williams MG,Rode LJ

    更新日期:1968-01-01 00:00:00

  • Analysis of Halobacterium halobium gas vesicles.

    abstract::Gas vesicles, isolated from lysed Halobacterium halobium cells, gave an amino acid analysis which accounted for 78% of the weight, and the balance was mainly salt and water. One percent of tightly bound d-galactose was found, as well as 2% of phosphate that was not released by treatment which promotes beta-elimination...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.114.3.1058-1067.1973

    authors: Krantz MJ,Ballou CE

    更新日期:1973-06-01 00:00:00

  • Role of NAD in regulating the adhE gene of Escherichia coli.

    abstract::The fermentative alcohol dehydrogenase of Escherichia coli is encoded by the adhE gene, which is induced under anaerobic conditions but repressed in air. Previous work suggested that induction of adhE might depend on NADH levels. We therefore directly measured the NAD+ and NADH levels for cultures growing aerobically ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.20.6013-6018.1996

    authors: Leonardo MR,Dailly Y,Clark DP

    更新日期:1996-10-01 00:00:00

  • Insertional inactivation of trpC in cloned Bacillus trp segments: evidence for a polar effect on trpF.

    abstract::Plasmid pUB110 was previously used as a vector to clone fragments of deoxyribonucleic acid that complement the trpC2 mutation in Bacillus subtilis from endonuclease EcoRI digested B. licheniformis, B. pumilus, and B. subtilis cellular deoxyribonucleic acid. Each of several such trp plasmids was subsequently shown to c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.3.1001-1006.1979

    authors: Keggins KM,Lovett PS,Marrero R,Hoch SO

    更新日期:1979-09-01 00:00:00

  • Inactivation of Lgt allows systematic characterization of lipoproteins from Listeria monocytogenes.

    abstract::Lipoprotein anchoring in bacteria is mediated by the prolipoprotein diacylglyceryl transferase (Lgt), which catalyzes the transfer of a diacylglyceryl moiety to the prospective N-terminal cysteine of the mature lipoprotein. Deletion of the lgt gene in the gram-positive pathogen Listeria monocytogenes (i) impairs intra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00976-06

    authors: Baumgärtner M,Kärst U,Gerstel B,Loessner M,Wehland J,Jänsch L

    更新日期:2007-01-01 00:00:00

  • Mutants suppressing novobiocin hypersensitivity of a mukB null mutation.

    abstract::The mukB gene is essential for the partitioning of sister chromosomes in Escherichia coli. A mukB null mutant is hypersensitive to the DNA gyrase inhibitor novobiocin. In this work, we isolated mutants suppressing the novobiocin hypersensitivity of the mukB null mutation. All suppressor mutations are localized in or n...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.13.3690-3695.2003

    authors: Adachi S,Hiraga S

    更新日期:2003-07-01 00:00:00

  • RNase III processing of intervening sequences found in helix 9 of 23S rRNA in the alpha subclass of Proteobacteria.

    abstract::We provide experimental evidence for RNase III-dependent processing in helix 9 of the 23S rRNA as a general feature of many species in the alpha subclass of Proteobacteria (alpha-Proteobacteria). We investigated 12 Rhodobacter, Rhizobium, Sinorhizobium, Rhodopseudomonas, and Bartonella strains. The processed region is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.17.4719-4729.2000

    authors: Evguenieva-Hackenberg E,Klug G

    更新日期:2000-09-01 00:00:00

  • Identification of the type I trimethoprim-resistant dihydrofolate reductase specified by the Escherichia coli R-plasmid R483: comparison with procaryotic and eucaryotic dihydrofolate reductases.

    abstract::We have isolated and determined the nucleotide sequence of a 1,626-base-pair fragment from R-plasmid R483 which encodes a trimethoprim-resistant dihydrofolate reductase. Analysis of the nucleotide sequence of this fragment revealed the presence of two open reading frames, each sufficient to encode polypeptides of appr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.3.1001-1008.1983

    authors: Simonsen CC,Chen EY,Levinson AD

    更新日期:1983-09-01 00:00:00

  • Dynamics of the SetCD-regulated integration and excision of genomic islands mobilized by integrating conjugative elements of the SXT/R391 family.

    abstract::Mobilizable genomic islands (MGIs) are small genomic islands that are mobilizable by SXT/R391 integrating conjugative elements (ICEs) due to similar origins of transfer. Their site-specific integration and excision are catalyzed by the integrase that they encode, but their conjugative transfer entirely depends upon th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01093-12

    authors: Daccord A,Mursell M,Poulin-Laprade D,Burrus V

    更新日期:2012-11-01 00:00:00

  • An epitope of elongation factor Tu is widely distributed within the bacterial and archaeal domains.

    abstract::A monoclonal antibody (MAb), MAb 900, which detects a 43-kDa protein present on Escherichia coli was found. Subsequently, more than 90 organisms, belonging to either the bacterial, archaeal, or eucaryal domain, were tested for reactivity to this MAb. Of the bacterial and archaeal domains, almost all species proved to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.1.11-19.1995

    authors: Weber S,Lottspeich F,Köhl J

    更新日期:1995-01-01 00:00:00

  • Selective depletion of Sulfolobus solfataricus transcription factor E under heat shock conditions.

    abstract::Archaeal transcriptional machinery is similar to that of eukaryotes. We studied the fates of various components of the Sulfolobus solfataricus transcriptional apparatus under different stresses and found that in cells incubated at 90 degrees C for 1 h, transcription factor E (TFE) is selectively depleted, but its mRNA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01534-09

    authors: Iqbal J,Qureshi SA

    更新日期:2010-06-01 00:00:00

  • Peptide wrwycr inhibits the excision of several prophages and traps holliday junctions inside bacteria.

    abstract::Peptide inhibitors of phage lambda site-specific recombination were previously isolated by screening synthetic combinatorial peptide libraries. These inhibitors cause the accumulation of complexes between the recombinase and the Holliday junction intermediate of several highly divergent tyrosine recombinases. Peptide ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01559-08

    authors: Gunderson CW,Boldt JL,Authement RN,Segall AM

    更新日期:2009-04-01 00:00:00

  • A conserved histidine in cytochrome c maturation permease CcmB of Shewanella putrefaciens is required for anaerobic growth below a threshold standard redox potential.

    abstract::Shewanella putrefaciens strain 200 respires a wide range of compounds as terminal electron acceptor. The respiratory versatility of Shewanella is attributed in part to a set of c-type cytochromes with widely varying midpoint redox potentials (E'(0)). A point mutant of S. putrefaciens, originally designated Urr14 and h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01249-06

    authors: Dale JR,Wade R Jr,Dichristina TJ

    更新日期:2007-02-01 00:00:00

  • Methylation of the cellular lipid of methionine-requiring Agrobacterium tumefaciens.

    abstract::Mutants of Agrobacterium tumefaciens requiring methionine for growth on a solid basal medium were induced by the use of N-methyl-N'-nitro-N-nitrosoguanidine. In addition to the difference of mutant strains, the extent of methionine dependency differed in a liquid basal medium and in the presence of aspartate or fumara...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.6.2078-2082.1968

    authors: Kaneshiro T

    更新日期:1968-06-01 00:00:00

  • Purification and characterization of an exo-beta-1,4-glucanase from Ruminococcus flavefaciens FD-1.

    abstract::An exo-beta-1,4-glucanase (Exo A) from Ruminococcus flavefaciens FD-1 was purified to homogeneity and characterized. Enzyme activity was monitored during purification by using the substrate p-nitrophenyl-beta-D-cellobioside (NPC). Over 85% of the NPC activity was found to be extracellular once the filter paper was deg...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.10.4581-4588.1987

    authors: Gardner RM,Doerner KC,White BA

    更新日期:1987-10-01 00:00:00

  • The recombinase IntA is required for excision of esp-containing ICEEfm1 in Enterococcus faecium.

    abstract::Comparative genome analysis of Enterococcus faecium recently revealed that a genomic island containing the esp gene, referred to as the esp-containing pathogenicity island (esp PAI), can be transferred by conjugation and contains a partial Tn916-like element and an integrase gene, intA. Here, we characterize the role ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00952-10

    authors: Top J,Sinnige JC,Majoor EA,Bonten MJ,Willems RJ,van Schaik W

    更新日期:2011-02-01 00:00:00

  • Deoxyribonucleic acid modifications and restriction endonuclease production in Neisseria gonorrhoeae.

    abstract::Modification of gonococcal deoxyribonucleic acid (DNA) was investigated, and the relationship with endonuclease production was explored. Both chromosomal and plasmid DNA from different gonococcal strains, irrespective of their plasmid content, was poorly cleaved by the restriction endonucleases HaeII, HaeIII, SacII, a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.2.788-795.1981

    authors: Norlander L,Davies JK,Hagblom P,Normark S

    更新日期:1981-02-01 00:00:00

  • EFFECT OF TUNGSTATE ON THE UPTAKE AND FUNCTION OF MOLYBDATE IN AZOTOBACTER AGILIS.

    abstract::Bulen, William A. (Charles F. Kettering Foundation, Yellow Springs, Ohio). Effect of tungstate on the uptake and function of molybdate in Azotobacter agilis. J. Bacteriol. 82:130-134. 1961.-The reported competitive inhibition of molybdate by tungstate was investigated in an effort to elucidate molybdenum functions ass...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.1.130-134.1961

    authors: Bulen WA

    更新日期:1961-07-01 00:00:00

  • An essential iteron-binding protein required for plasmid R1162 replication induces localized melting within the origin at a specific site in AT-rich DNA.

    abstract::The R1162-encoded protein RepIB is essential for replication of the plasmid and binds specifically to iterons within the replicative origin. The protein causes the localized melting of DNA (determined by sensitivity to P1 nuclease) at a site within the AT-rich region of the origin, about 60 bp from the iteron binding ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.17.5539-5545.1991

    authors: Kim YJ,Meyer RJ

    更新日期:1991-09-01 00:00:00

  • Molecular cloning of genes involved with expression of A-band lipopolysaccharide, an antigenically conserved form, in Pseudomonas aeruginosa.

    abstract::Most strains of Pseudomonas aeruginosa can express two chemically and immunologically distinct types of lipopolysaccharide (LPS), an antigenically conserved form called A band and the serotype-specific form called B band. To study the molecular controls regulating expression of the A-band LPS antigen, we have cloned t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.18.5624-5630.1991

    authors: Lightfoot J,Lam JS

    更新日期:1991-09-01 00:00:00

  • A Cardiolipin-Deficient Mutant of Rhodobacter sphaeroides Has an Altered Cell Shape and Is Impaired in Biofilm Formation.

    abstract:UNLABELLED:Cell shape has been suggested to play an important role in the regulation of bacterial attachment to surfaces and the formation of communities associated with surfaces. We found that a cardiolipin synthase (Δcls) mutant of the rod-shaped bacterium Rhodobacter sphaeroides--in which synthesis of the anionic, h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00420-15

    authors: Lin TY,Santos TM,Kontur WS,Donohue TJ,Weibel DB

    更新日期:2015-11-01 00:00:00

  • Genetic and biochemical analysis of the isoenzymes concerned in the first reaction of aromatic biosynthesis in Escherichia coli.

    abstract::Mutant strains of Escherichia coli K-12 were isolated possessing mutations which affected the tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase, the phenylalanine-inhibitable DAHP synthetase, or the tryptophan-repressible DAHP synthetase. The mutations causing the loss of each of th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.93.1.237-244.1967

    authors: Wallace BJ,Pittard J

    更新日期:1967-01-01 00:00:00

  • Essential role of flavohemoglobin in long-term anaerobic survival of Bacillus subtilis.

    abstract::A Bacillus subtilis culture incubated anaerobically in nitrate-containing medium lost viability during the first 3 days but recovered thereafter. A flavohemoglobin mutant showed very poor survival under these conditions unless the cells were prevented from carrying out nitrate respiration. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00557-06

    authors: Nakano MM

    更新日期:2006-09-01 00:00:00

  • Impaired expression of certain prereplicative bacteriophage T4 genes explains impaired T4 DNA synthesis in Escherichia coli rho (nusD) mutants.

    abstract::The Escherichia coli rho 026 mutation that alters the transcription termination protein Rho prevents growth of wild-type bacteriophage T4. Among the consequences of this mutation are delayed and reduced T4 DNA replication. We show that these defects can be explained by defective synthesis of certain T4 replication-rec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.3872-3880.1989

    authors: Stitt BL,Mosig G

    更新日期:1989-07-01 00:00:00

  • The Clostridium difficile cpr locus is regulated by a noncontiguous two-component system in response to type A and B lantibiotics.

    abstract::The intestinal pathogen Clostridium difficile is known to grow only within the intestines of mammals, yet little is known about how the bacterium subsists in this environment. In the intestine, C. difficile must contend with innate defenses within the host, such as cationic antimicrobial peptides (CAMPs) produced by t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00166-13

    authors: Suárez JM,Edwards AN,McBride SM

    更新日期:2013-06-01 00:00:00

  • Klebsiella serotype 25 capsular polysaccharide: primary structure and depolymerization by a bacteriophage-borne glycanase.

    abstract::By partial acid hydrolysis, methylation and gas-liquid chromatography-mass spectrometry of the methylated monomers (as the alditol acetates), mass spectrometry of trimethylsilylated disaccharide alditols, as well as proton magnetic resonance, the primary structure of the Klebsiella serotype 25 capsular polysaccharide ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.366-374.1977

    authors: Niemann H,Kwiatkowski B,Westphal U,Stirm S

    更新日期:1977-04-01 00:00:00

  • Archaeal DNA polymerase D but not DNA polymerase B is required for genome replication in Thermococcus kodakarensis.

    abstract::Three evolutionarily distinct families of replicative DNA polymerases, designated polymerase B (Pol B), Pol C, and Pol D, have been identified. Members of the Pol B family are present in all three domains of life, whereas Pol C exists only in Bacteria and Pol D exists only in Archaea. Pol B enzymes replicate eukaryoti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02037-12

    authors: Cubonová L,Richardson T,Burkhart BW,Kelman Z,Connolly BA,Reeve JN,Santangelo TJ

    更新日期:2013-05-01 00:00:00

  • Identification of a bacterial sensing protein and effects of its elevated expression.

    abstract::The Escherichia coli flaA gene product (also called cheC) plays a crucial role in switching flagellar rotational direction during chemotactic responses. Wild-type and mutant alleles have been cloned onto plasmid vectors, and the gene product has been identified as a 37,000-dalton protein. The flaA product appeared as ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.1.398-405.1985

    authors: Clegg DO,Koshland DE Jr

    更新日期:1985-04-01 00:00:00