Abstract:
:Tn2603 is a multiple-resistance transposon encoding resistance to ampicillin, streptomycin, sulfonamide, and mercury and having a molecular size of 20 kilobase pairs, with 200-base-pair inverted repeats at both ends. The essential sites and functions of Tn2603 which are required for its transposition were determined through construction and characterization of various deletion mutants affecting the efficiency of transposition. Deletions were introduced in plasmid pMK1::Tn2603 by partial digestion with restriction endonuclease EcoRI in vitro. Analysis of deletion mutants showed that the inverted repeat segments at both ends of the trans-acting diffusible product(s) encoded in the right-hand side of the central portion were required for the transposition of Tn2603. An essential gene product was revealed as a protein having a molecular weight of 110,000 by analysis of polypeptides synthesized in Escherichia coli minicells. This protein was assumed to be the so-called transposase.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Tanaka M,Harafuji H,Yamamoto Tdoi
10.1128/JB.151.2.723-728.1982subject
Has Abstractpub_date
1982-08-01 00:00:00pages
723-8issue
2eissn
0021-9193issn
1098-5530journal_volume
151pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2003-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1975-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:1996-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1978-01-01 00:00:00
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更新日期:2000-11-01 00:00:00
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doi:10.1128/jb.176.7.1939-1943.1994
更新日期:1994-04-01 00:00:00
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更新日期:1998-10-01 00:00:00
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更新日期:2000-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.2.620-624.1979
更新日期:1979-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1979-01-01 00:00:00