Abstract:
:Pasteurella multocida is the causative agent of a range of diseases with economic importance in production animals. Many systems have been employed to identify virulence factors of P. multocida, including in vivo expression technology (IVET), signature-tagged mutagenesis, and whole-genome expression profiling. In a previous study in which IVET was used with P. multocida, nrfE was identified as a gene that is preferentially expressed in vivo. In Escherichia coli, nrfE is part of the formate-dependent nitrite reductase system involved in utilizing available nitrite as an electron accepter during growth under anaerobic conditions. In this study, we constructed an isogenic P. multocida strain that was unable to reduce nitrite under either aerobic or anaerobic conditions, thereby demonstrating that P. multocida nrfE is essential for nitrite reduction. However, the nrfE mutant was still virulent in mice. Real-time reverse transcription-PCR analysis indicated that nrfE was regulated independently of nrfABCD by an independent promoter that is likely to be upregulated in vivo.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Boucher DJ,Adler B,Boyce JDdoi
10.1128/JB.187.7.2278-2285.2005keywords:
subject
Has Abstractpub_date
2005-04-01 00:00:00pages
2278-85issue
7eissn
0021-9193issn
1098-5530pii
187/7/2278journal_volume
187pub_type
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更新日期:1976-01-01 00:00:00
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更新日期:1966-02-01 00:00:00