The Pasteurella multocida nrfE gene is upregulated during infection and is essential for nitrite reduction but not for virulence.

Abstract:

:Pasteurella multocida is the causative agent of a range of diseases with economic importance in production animals. Many systems have been employed to identify virulence factors of P. multocida, including in vivo expression technology (IVET), signature-tagged mutagenesis, and whole-genome expression profiling. In a previous study in which IVET was used with P. multocida, nrfE was identified as a gene that is preferentially expressed in vivo. In Escherichia coli, nrfE is part of the formate-dependent nitrite reductase system involved in utilizing available nitrite as an electron accepter during growth under anaerobic conditions. In this study, we constructed an isogenic P. multocida strain that was unable to reduce nitrite under either aerobic or anaerobic conditions, thereby demonstrating that P. multocida nrfE is essential for nitrite reduction. However, the nrfE mutant was still virulent in mice. Real-time reverse transcription-PCR analysis indicated that nrfE was regulated independently of nrfABCD by an independent promoter that is likely to be upregulated in vivo.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Boucher DJ,Adler B,Boyce JD

doi

10.1128/JB.187.7.2278-2285.2005

keywords:

subject

Has Abstract

pub_date

2005-04-01 00:00:00

pages

2278-85

issue

7

eissn

0021-9193

issn

1098-5530

pii

187/7/2278

journal_volume

187

pub_type

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