Abstract:
:An apparent operator-constitutive mutation was discovered in the repressible acid phosphatase system in Saccharomyces cerevisiae. The site of mutation, designated PHOO, was found to be closely linked to the phoD locus. The mutant allele, PHOO, was semidominant over the wild-type allele and effective for the expression of the phoD gene in cis position. The phoD mutation gave rise to a defective phenotype for the formation of the repressible acid phosphatase. On the other hand, neither the repressible acid phosphatase activity in the cell-free extracts prepared from cells of the temperature-sensitive phoD mutant grown at 25 C, nor that of the revertants from the phoD mutants, could be distinguished from that of the wild-type strain with respect to thermolability and K(m) value for p-nitrophenylphosphate. These results strongly suggest that the phoD gene is not a structural gene, but a regulatory gene exerting positive control for the formation of repressible acid phosphatase. Close similarity between the apparent role of the phoO-PHOD gene cluster and that of the c-GAL4 gene cluster in the galactose system of S. cerevisiae could be inferred.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Toh-E A,Oshima Ydoi
10.1128/JB.120.2.608-617.1974subject
Has Abstractpub_date
1974-11-01 00:00:00pages
608-17issue
2eissn
0021-9193issn
1098-5530journal_volume
120pub_type
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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doi:10.1128/JB.147.2.340-349.1981
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.5904-5911.1994
更新日期:1994-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1974-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1999-12-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1976-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.178.15.4335-4343.1996
更新日期:1996-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-09-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/JB.112.1.142-147.1972
更新日期:1972-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.2.303-316.1998
更新日期:1998-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.71-77.1969
更新日期:1969-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1993-04-01 00:00:00
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更新日期:1988-11-01 00:00:00
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更新日期:1970-04-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.82.6.898-904.1961
更新日期:1961-12-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:2008-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1998-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.6.1862-1866.1991
更新日期:1991-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1997-09-01 00:00:00
abstract::Lactobacillus rhamnosus MTCC 5462 was isolated from infant gastrointestinal flora. The strain exhibited an ability to reduce cholesterol and stimulate immunity. The strain has exhibited positive results in alleviating gastrointestinal discomfort and good potential as a probiotic. We sequenced the whole genome of the s...
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pub_type: 杂志文章
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更新日期:2012-03-01 00:00:00