Cloning and sequencing of the beta-lactam hydroxylase gene (cefF) from Streptomyces clavuligerus: gene duplication may have led to separate hydroxylase and expandase activities in the actinomycetes.

abstract：:Procedures were developed to facilitate the identification of genes that belong to a given regulon and characterization of their responses to the regulator. The regulon controlled by the Escherichia coli leucine-responsive regulatory protein (Lrp) was studied by isolating random transcriptional fusions to lacZ, using ...

journal_title：Journal of bacteriology

authors： Bhagwat SP,Rice MR,Matthews RG,Blumenthal RM

更新日期：1997-10-01 00:00:00

abstract：:The hemA mutation of Escherichia coli K-12 confers a requirement for delta-aminolevulinic acid (ALA). Cell extract prepared from the hemA strain SASX41B was incapable of producing ALA from either glutamate or glutamyl-tRNA, whereas extract of the hem+ strain HB101 formed colorimetrically detectable amounts of ALA and ...

journal_title：Journal of bacteriology

authors： Avissar YJ,Beale SI

更新日期：1989-06-01 00:00:00

abstract：:Membrane fragments containing diacyl phospholipids were released from viable cells of Haemophilus parainfluenzae during incubation in ethylenediaminetetraacetic acid (EDTA)- tris(hydroxymethyl)aminomethane (Tris) buffer. The phospholipids located in the part of the membrane that was released during the EDTA-Tris treat...

journal_title：Journal of bacteriology

authors： Tucker AN,White DC

更新日期：1970-08-01 00:00:00

abstract：:Evidence from pulse-chase experiments indicates that the outer membrane matrix protein or porin of Escherichia coli B/r passes through a Sarkosyl-soluble membrane pool on the way to its eventual Sarkosyl-insoluble state in the outer membrane. ...

journal_title：Journal of bacteriology

authors： Boyd A,Holland IB

更新日期：1980-09-01 00:00:00

abstract：:The syrB gene is required for syringomycin production by Pseudomonas syringae pv. syringae and full virulence during plant pathogenesis. Strain B3AR132 containing a syrB::lacZ fusion was used to detect transcriptional activation of the syrB gene in syringomycin minimal medium by plant metabolites with signal activity....

journal_title：Journal of bacteriology

authors： Mo YY,Gross DC

更新日期：1991-09-01 00:00:00

abstract：:ClpX is a well-characterized bacterial chaperone that plays a role in many processes, including protein turnover and the remodeling of macromolecular complexes. All of these activities require ATP hydrolysis-dependent, ClpX-mediated protein unfolding. Here we used site-directed mutagenesis in combination with genetics...

journal_title：Journal of bacteriology

authors： Haeusser DP,Lee AH,Weart RB,Levin PA

更新日期：2009-03-01 00:00:00

abstract：:In a previous study (J. O'Rear, L. Alberti, and R. M. Harshey, J. Bacteriol. 174:6125-6137, 1992) we reported the isolation of several transposon mutants of Serratia marcescens 274 that were defective either in swarming alone or in both swimming and swarming motility. All the nonflagellate (Fla-) mutants, while defect...

journal_title：Journal of bacteriology

authors： Matsuyama T,Bhasin A,Harshey RM

更新日期：1995-02-01 00:00:00

abstract：:The Marburg strain of Butyribacterium methylotrophicum did not grow on CO alone but did consume CO during growth on a variety of substrates in the presence of a 100% CO gas phase. We selected a strain (the CO strain) that grew vigorously on CO alone. The ability of the CO strain to grow on CO was stable through multip...

journal_title：Journal of bacteriology

authors： Lynd L,Kerby R,Zeikus JG

更新日期：1982-01-01 00:00:00

abstract：:Three groups of linked markers were mapped in Bacillus thuringiensis 4042B by using two-, three-, and four-factor crosses mediated by the temperate bacteriophages TP-13 and TP-18. The order of markers was (trp-11, trp-2)-(leu-1, leu-2)-his-1-(lys-1, lys-2)-cys-1 in the first group; met-1-(argCl, argOl)-met-2-(pyr-1, p...

journal_title：Journal of bacteriology

authors： Barsomian GD,Robillard NJ,Thorne CB

更新日期：1984-03-01 00:00:00

abstract：:Wild-type Escherichia coli cannot grow on L-1,2-propanediol; mutants that can do so have increased basal activity of an NAD-linked L-1,2-propanediol oxidoreductase. This enzyme belongs to the L-fucose system and functions normally as L-lactaldehyde reductase during fermentation of the methylpentose. In wild-type cells...

journal_title：Journal of bacteriology

authors： Chen YM,Chakrabarti T,Lin EC

更新日期：1984-08-01 00:00:00

abstract：:Pseudomonas putida DOT-T1 was isolated after enrichment on minimal medium with 1% (vol/vol) toluene as the sole C source. The strain was able to grow in the presence of 90% (vol/vol) toluene and was tolerant to organic solvents whose log P(ow) (octanol/water partition coefficient) was higher than 2.3. Solvent toleranc...

journal_title：Journal of bacteriology

authors： Ramos JL,Duque E,Huertas MJ,Haïdour A

更新日期：1995-07-01 00:00:00

abstract：:Polyglutamate is found in various bacteria, but displays different functions depending on the species and their environment. Here, we describe a minimal polyglutamate synthesis system in Bacillus anthracis. In addition to the three genes previously described as sufficient for polyglutamate synthesis, this system inclu...

journal_title：Journal of bacteriology

authors： Candela T,Mock M,Fouet A

更新日期：2005-11-01 00:00:00

abstract：:The guanine plus cytosine (GC) content of the deoxyribonucleic acid (DNA) of 11 isolates of host-dependent (H-D) bdellovibrios and 18 host-independent (H-I) derivatives was determined from thermal denaturation curves and buoyant densities in CsCl. The H-D and respective H-I cultures have GC contents which are identica...

journal_title：Journal of bacteriology

authors： Seidler RJ,Starr MP,Mandel M

更新日期：1969-11-01 00:00:00

abstract：:Previously, the effects of three point mutations (at amino acid positions 31, 84, and 194) in the gene coding for a sulfonamide-resistant dihydropteroate synthase of Neisseria meningitidis were analyzed by site-directed mutagenesis. Changes at positions 31 and 194 abolished the phenotypic expression of sulfonamide res...

journal_title：Journal of bacteriology

authors： Fermér C,Swedberg G

更新日期：1997-02-01 00:00:00

abstract：:Hoffman, Heiner (New York University, New York, N.Y.), Jon Valdina, and Michael E. Frank. Effects of high incubation temperature upon the cell wall of Escherichia coli. J. Bacteriol. 91:1635-1637. 1966.-Escherichia coli cells grown at 45 C were found to swell when suspended in distilled water, and were broken down whe...

journal_title：Journal of bacteriology

authors： Hoffman H,Valdina J,Frank ME

更新日期：1966-04-01 00:00:00

abstract：:Total DNA of various Rhizobium galegae strains representing different geographical origins, and taxonomic divergence was digested with three restriction enzymes separately, Southern blotted, and hybridized with six heterologous probes. The sequence divergences for different pairwise comparisons were calculated from pr...

journal_title：Journal of bacteriology

authors： Kaijalainen S,Lindström K

更新日期：1989-10-01 00:00:00

abstract：:Strain DCB-1 is a strict anaerobe capable of reductive dehalogenation. We elucidated metabolic processes in DCB-1 which may be related to dehalogenation and which further characterize the organism physiologically. Sulfoxy anions and CO2 were used by DCB-1 as catabolic electron acceptors. With suitable electron donors,...

journal_title：Journal of bacteriology

authors： Mohn WW,Tiedje JM

更新日期：1990-04-01 00:00:00

abstract：:The papA gene of the uropathogenic strain Escherichia coli J96, coding for the Pap pili subunit, was subjected to DNA sequencing, and found to code for an 185-amino acid-long polypeptide with a 22-amino acid-long signal peptide. Here we present the primary sequence, the hydrophilicity profile, and the predicted polype...

journal_title：Journal of bacteriology

authors： Båga M,Normark S,Hardy J,O'Hanley P,Lark D,Olsson O,Schoolnik G,Falkow S

更新日期：1984-01-01 00:00:00

abstract：:Polyphosphate of average chain length corresponding to 10 phosphate units was detected in the mycelial extract of zygomycetes. Gel electrophoresis techniques commonly used for the separation and characterization of acidic mucopolysaccharides were successfully used for the detection, purification, and characterization ...

journal_title：Journal of bacteriology

authors： Dietrich SM

更新日期：1976-09-01 00:00:00

abstract：:Despite the fact that phosphoenolpyruvate carboxylase (PEPC) activity has been measured and in some cases even purified from some Archaea, the gene responsible for this activity has not been elucidated. Using sensitive sequence comparison methods, we detected a highly conserved, uncharacterized archaeal gene family th...

journal_title：Journal of bacteriology

authors： Ettema TJ,Makarova KS,Jellema GL,Gierman HJ,Koonin EV,Huynen MA,de Vos WM,van der Oost J

更新日期：2004-11-01 00:00:00

abstract：:The formation of recombinants in Hfr crosses was studied in Escherichia coli strains carrying combinations of genes known to affect recombination and DNA repair. Mutations in ruv and recG eliminate activities that have been shown to process Holliday junction intermediates by nuclease cleavage and/or branch migration. ...

journal_title：Journal of bacteriology

authors： Ryder L,Whitby MC,Lloyd RG

更新日期：1994-03-01 00:00:00

abstract：:Chlamydia trachomatis lacks the canonical genes required for the biosynthesis of p-aminobenzoate (pABA), a component of essential folate cofactors. Previous studies revealed a single gene from C. trachomatis, the CT610 gene, that rescues Escherichia coli ΔpabA, ΔpabB, and ΔpabC mutants, which are otherwise auxotrophic...

journal_title：Journal of bacteriology

authors： Macias-Orihuela Y,Cast T,Crawford I,Brandecker KJ,Thiaville JJ,Murzin AG,de Crécy-Lagard V,White RH,Allen KD

更新日期：2020-09-23 00:00:00

abstract：:The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

journal_title：Journal of bacteriology

authors： Katagiri M,Wheelis ML

更新日期：1971-05-01 00:00:00

abstract：:Streptococcus salivarius, a gram-positive bacterium found in the human oral cavity, expresses flexible peritrichous fimbriae. In this paper, we report purification and partial characterization of S. salivarius fimbriae. Fimbriae were extracted by shearing the cell surface of hyperfimbriated mutant A37 (a spontaneous m...

journal_title：Journal of bacteriology

authors： Lévesque C,Vadeboncoeur C,Chandad F,Frenette M

更新日期：2001-05-01 00:00:00

abstract：:The antibacterial protein toxin colicin V is secreted from Escherichia coli cells by a dedicated export system that is a member of the multicomponent ATP-binding cassette (ABC) transporter family. At least three proteins, CvaA, CvaB, and TolC, are required for secretion via this signal sequence-independent pathway. In...

journal_title：Journal of bacteriology

authors： Skvirsky RC,Reginald S,Shen X

更新日期：1995-11-01 00:00:00

abstract：:Acetate kinase (ACK) converts acetyl phosphate to acetate along with the generation of ATP in the pathway for mixed-acid fermentation in Lactococcus lactis. The reverse reaction yields acetyl phosphate for assimilation purposes. Remarkably, L. lactis has two ACK isozymes, and the corresponding genes are present in an ...

journal_title：Journal of bacteriology

authors： Puri P,Goel A,Bochynska A,Poolman B

更新日期：2014-04-01 00:00:00

abstract：:Smith, Paul F. (University of Pennsylvania, Philadelphia) and George H. Rothblat. Comparison of pleuropneumonia-like and L-type organisms. J. Bacteriol. 83:500-506. 1962.-The content of total lipid and nonsaponifiable lipid depended upon the type of organism; the greatest amounts were found in pleuropneumonia-like org...

journal_title：Journal of bacteriology

authors： SMITH PF,ROTHBLAT GH

更新日期：1962-03-01 00:00:00

abstract：:Levin, Alfred P. (The Johns Hopkins University, Baltimore, Md.), and Philip E. Hartman. Action of a histidine analogue, 1,2,4-triazole-3-alanine, in Salmonella typhimurium. J. Bacteriol. 86:820-828. 1963.-The effect of the histidine analogue, 1,2,4-triazole-3-alanine (TRA), on growth and enzyme synthesis in histidine ...

journal_title：Journal of bacteriology

authors： LEVIN AP,HARTMAN PE

更新日期：1963-10-01 00:00:00

abstract：:The disaccharide trehalose is accumulated as a storage product by spores of Streptomyces griseus. Nongerminating spores used their trehalose reserves slowly when incubated in buffer for several months. In contrast, spores rapidly depleted their trehalose pools during the first hours of germination. Extracts of dormant...

journal_title：Journal of bacteriology

authors： McBride MJ,Ensign JC

更新日期：1987-11-01 00:00:00

abstract：:The ability of deoxyribonucleic acid (DNA) isolated from a number of actinomycetes to reassociate with reference DNA from Actinoplanes philippinensis or Streptomyces venezuelae has been measured. All of the DNA preparations except for those from Nocardia erythropolis and Thermomonospora viridis contained 70 to 73 mole...

journal_title：Journal of bacteriology

authors： Farina G,Bradley SG

更新日期：1970-04-01 00:00:00