Chemotaxis toward amino acids in Escherichia coli.

abstract：:Lysyl-transfer ribonucleic acid synthetase (EC 6.1.1.6) was identified as four polypeptide spots after two-dimensional polyacrylamide gel electrophoresis of whole-cell lysates of Escherichia coli. Identification was made by migration with partially purified enzyme preparations, by peptide map patterns, by mutant analy...

journal_title：Journal of bacteriology

authors： Hirshfield IN,Bloch PL,Van Bogelen RA,Neidhardt FC

更新日期：1981-04-01 00:00:00

abstract：:Ribonucleotide reductases (RNRs) are crucial to all living cells, since they provide deoxyribonucleotides (dNTPs) for DNA synthesis and repair. In Mycobacterium tuberculosis, a class Ib RNR comprising nrdE- and nrdF2-encoded subunits is essential for growth in vitro. Interestingly, the genome of this obligate human pa...

journal_title：Journal of bacteriology

authors： Mowa MB,Warner DF,Kaplan G,Kana BD,Mizrahi V

更新日期：2009-02-01 00:00:00

abstract：:A spontaneous lac+ revertant of an adenylate cyclase deletion strain of Escherichia coli K-12 was isolated and characterized. This revertant, designated strain KC20, exhibited a pleiotropic suppression of the adenylate cyclase defect, with the crp locus being the site of the suppressor mutation. Cyclic adenosine 3',5'...

journal_title：Journal of bacteriology

authors： Bankaitis VA,Kline EL

更新日期：1981-08-01 00:00:00

abstract：:As with other inducible enzymes, the induced synthesis of l-arabinose isomerase (l-arabinose ketol isomerase, EC 5.3.1.4) in Salmonella typhimurium is subject to catabolite repression. Of the three catabolite repressors tested, glucose produces maximum repression. Analogues of catabolite repressors like 2-deoxy-d-gluc...

journal_title：Journal of bacteriology

authors： Bhattacharya AK,Chakravorty M

更新日期：1971-04-01 00:00:00

abstract：:An in vitro constructed plasmid, pVH15, consisting of the entire genome of the plasmid ColE1, the tryptophan operon of Escherichia coli, and regions of the bacteriophage PHI80pt190, spontaneously gave rise in E. coli to a mini-ColE1 plasmid consisting of approximately one-half of the ColE1 genome and a small segment o...

journal_title：Journal of bacteriology

authors： Hershfield V,Boyer HW,Chow L,Helinski DR

更新日期：1976-04-01 00:00:00

abstract：:Abram, Dinah (Purdue University, Lafayette, Ind.), A. E. Vatter, and Henry Koffler. Attachment and structural features of flagella of certain bacilli. J. Bacteriol. 91:2045-2068. 1966.-The attachment of flagella to cells of various mesophilic and thermophilic strains of Bacillus was studied electron microscopically. S...

journal_title：Journal of bacteriology

authors： Abram D,Vatter AE,Koffler H

更新日期：1966-05-01 00:00:00

abstract：:The 5,10-methylenetetrahydrofolate dehydrogenase of heterotrophically grown Peptostreptococcus productus Marburg was purified to apparent homogeneity. The purified enzyme catalyzed the reversible oxidation of methylenetetrahydrofolate with NADP+ as the electron acceptor at a specific activity of 627 U/mg of protein. T...

journal_title：Journal of bacteriology

authors： Wohlfarth G,Geerligs G,Diekert G

更新日期：1991-02-01 00:00:00

abstract：:Cysteine and methionine availability influences many processes in the cell. In bacteria, transcription of the specific genes involved in the synthesis of these two amino acids is usually regulated by different mechanisms or regulators. Pathways for the synthesis of cysteine and methionine and their interconversion wer...

journal_title：Journal of bacteriology

authors： Sperandio B,Polard P,Ehrlich DS,Renault P,Guédon E

更新日期：2005-06-01 00:00:00

abstract：:The function of SufI, a well-studied substrate of the TatABC translocase in Escherichia coli, is not known. It was earlier implicated in cell division, based on the finding that multiple copies of sufI suppressed the phenotypes of cells with mutations in ftsI (ftsI23), which encodes a divisomal transpeptidase. Recentl...

journal_title：Journal of bacteriology

authors： Samaluru H,SaiSree L,Reddy M

更新日期：2007-11-01 00:00:00

abstract：:A 4.2-kilobase cryptic Bacteroides plasmid, pB8-51, is found in several colonic Bacteroides species. To determine whether pB8-51 is mobilized by any of the known Bacteroides conjugal elements, we constructed an Escherichia coli-Bacteroides shuttle vector, pVAL-1, which contains pB8-51. We constructed Bacteroides unifo...

journal_title：Journal of bacteriology

authors： Valentine PJ,Shoemaker NB,Salyers AA

更新日期：1988-03-01 00:00:00

abstract：:The filamentous cyanobacterium Microcoleus vaginatusis found in arid land soils worldwide. The genome of M. vaginatus strain FGP-2 allows exploration of genes involved in photosynthesis, desiccation tolerance, alkane production, and other features contributing to this organism's ability to function as a major componen...

journal_title：Journal of bacteriology

authors： Starkenburg SR,Reitenga KG,Freitas T,Johnson S,Chain PS,Garcia-Pichel F,Kuske CR

更新日期：2011-09-01 00:00:00

abstract：:Late log-phase Escherichia coli B/r cells are 1.6 times more sensitive to killing by X rays than are stationary-phase cells when grown in Brain Heart Infusion (BHI) + glucose. The number of single-chain breaks formed per krad is the same for log- and stationary-phase cells. Stationary-phase cells show a somewhat great...

journal_title：Journal of bacteriology

authors： Town CD,Smith KC,Kaplan HS

更新日期：1971-01-01 00:00:00

abstract：:The broad-host-range plasmid RK2 is capable of replication and stable maintenance within a wide range of gram-negative bacterial hosts. It encodes the essential replication initiation protein TrfA, which binds to the host initiation protein, DnaA, at the plasmid origin of replication (oriV). There are two versions of ...

journal_title：Journal of bacteriology

authors： Kongsuwan K,Josh P,Picault MJ,Wijffels G,Dalrymple B

更新日期：2006-08-01 00:00:00

abstract：:Rod-shaped bacteria grow by a repetitive cycle of elongation followed by division, and the mechanisms responsible for these two processes have been studied for decades. However, little is known about what happens during the transition between the two activities. At least one event occurs after elongation ends and befo...

journal_title：Journal of bacteriology

authors： Potluri LP,Kannan S,Young KD

更新日期：2012-10-01 00:00:00

abstract：:Formate is an important energy substrate for sulfate-reducing bacteria in natural environments, and both molybdenum- and tungsten-containing formate dehydrogenases have been reported in these organisms. In this work, we studied the effect of both metals on the levels of the three formate dehydrogenases encoded in the ...

journal_title：Journal of bacteriology

authors： da Silva SM,Pimentel C,Valente FM,Rodrigues-Pousada C,Pereira IA

更新日期：2011-06-01 00:00:00

abstract：:The mechanism of action of diphtheria toxin in an Escherichia coli cell-free protein-synthesizing system was examined. When the washed ribosomes were incubated with toxin before addition of messenger ribonucleic acid (RNA), peptide syntheses of (14)C-phenylalanine directed by polyuridylic acid or phage R17 RNA were st...

journal_title：Journal of bacteriology

authors： Tsugawa A,Ohsumi Y,Kato I

更新日期：1970-10-01 00:00:00

abstract：:A number of gram-negative bacteria have a quorum-sensing system and produce N-acyl-l-homoserine lactone (AHL) that they use them as a quorum-sensing signal molecule. Pantoea ananatis is reported as a common colonist of wheat heads at ripening and causes center rot of onion. In this study, we demonstrated that P. anana...

journal_title：Journal of bacteriology

authors： Morohoshi T,Nakamura Y,Yamazaki G,Ishida A,Kato N,Ikeda T

更新日期：2007-11-01 00:00:00

abstract：:A mutant requiring isoleucine and valine for growth, because of the absence of acetolactate synthase activity, has been isolated. At least one of three different genes (ilvG, ilvB, ilvI) is required for the expression of acetolactate synthase activity, thus suggesting the presence of three different acetolactate synth...

journal_title：Journal of bacteriology

authors： Guardiola J,De Felice M,Iaccarino M

更新日期：1974-10-01 00:00:00

abstract：:Liquid holding recovery (LHR) in ultraviolet-irradiated Bacteroides fragilis cells occurred under aerobic conditions but was inhibited by anaerobic conditions. The increase in survival after aerobic LHR resulted in an increase in the shoulder regions of the ultraviolet survival curves. Maximum LHR was obtained after h...

journal_title：Journal of bacteriology

authors： Jones DT,Woods DR

更新日期：1981-01-01 00:00:00

abstract：:We constructed mercury resistance operon-luciferase (mer-lux) transcriptional fusion plasmids to evaluate in vivo gene expression rates of the mer structural gene promoter (PTPCAD) of transposon Tn21. In vivo gene expression kinetics corresponded well with those previously determined in vitro, yielding an apparent K0....

journal_title：Journal of bacteriology

authors： Condee CW,Summers AO

更新日期：1992-12-01 00:00:00

abstract：:Plasmids for high-level expression of penicillin-binding protein 6 (PBP6) were constructed, giving rise to overproduction of PBP6 under the control of the lambda pR promoter in either the periplasmic or the cytoplasmic space. In contrast to penicillin-binding protein 5 (PBP5), the presence of high amounts of PBP6 in t...

journal_title：Journal of bacteriology

authors： van der Linden MP,de Haan L,Hoyer MA,Keck W

更新日期：1992-12-01 00:00:00

abstract：:The addition of 5',5',5'-trifluoroleucine (fluoroleucine) to leucine auxotrophs of Salmonella typhimurium permitted protein but not ribonucleic acid (RNA) synthesis to continue after leucine depletion. The uncoupling of the formation of these macromolecules by fluoroleucine was apparent if RNA and protein synthesis wa...

journal_title：Journal of bacteriology

authors： Trela JM,Freundlich M

更新日期：1969-07-01 00:00:00

abstract：:The minimum sequence of the enzymatic (A) subunit of Shiga toxin (STX) required for activity was investigated by introducing N-terminal and C-terminal deletions in the molecule. Enzymatic activity was assessed by using an in vitro translation system. A 253-amino-acid STX A polypeptide, which is recognized as the enzym...

journal_title：Journal of bacteriology

authors： Haddad JE,al-Jaufy AY,Jackson MP

更新日期：1993-08-01 00:00:00

abstract：:Escherichia coli VC30 is a temperature-sensitive mutant which is defective in autolysis. Strain VC30 lyses at 30 degrees C when treated with beta-lactam antibiotics or D-cycloserine or when deprived of diaminiopimelic acid. The same treatments inhibit growth of the mutant at 42 degrees C but do not cause lysis. Strain...

journal_title：Journal of bacteriology

authors： Lubitz W,Harkness RE,Ishiguro EE

更新日期：1984-07-01 00:00:00

abstract：:The gene coding for alveolysin, the thiol-activated toxin produced by Bacillus alvei, has been cloned by means of an oligonucleotide based on the known N-terminal sequence of the secreted protein. The complete nucleotide sequence of the gene has been determined. The deduced amino acid sequence of alveolysin shows that...

journal_title：Journal of bacteriology

authors： Geoffroy C,Mengaud J,Alouf JE,Cossart P

更新日期：1990-12-01 00:00:00

abstract：:The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was i...

journal_title：Journal of bacteriology

authors： Laspia MF,Wallace SS

更新日期：1988-08-01 00:00:00

abstract：:In Salmonella typhimurium the two enzymes of proline catabolism, proline oxidase and Delta(1)-pyrroline-5-carboxylic acid dehydrogenase, are subject to catabolite repression when the cells are grown in the presence of glucose. Mutants partially relieved of catabolite repression (PutR) for the proline catabolic enzymes...

journal_title：Journal of bacteriology

authors： Newell SL,Brill WJ

更新日期：1972-08-01 00:00:00

abstract：:The lmaA gene of Listeria monocytogenes encodes a protein capable of inducing delayed-type hypersensitivity reactions in L. monocytogenes-immune mice (S. Göhmann, M. Leimeister-Wachter, E. Schiltz, W. Goebel, and T. Chakraborty, M. Microbiol. 4:1091-1099, 1990). Here we show that it is the last gene of the lma operon,...

journal_title：Journal of bacteriology

authors： Schäferkordt S,Chakraborty T

更新日期：1997-04-01 00:00:00

abstract：UNLABELLED:The latent period of phage T4, normally ∼25 min, can be extended indefinitely if the infected cell is superinfected after 5 min. This phenomenon, designated lysis inhibition (LIN), was first described in the 1940s and is genetically defined by mutations in diverse T4 r genes. RI, the main effector of LIN, ha...

journal_title：Journal of bacteriology

authors： Chen Y,Young R

更新日期：2016-08-25 00:00:00

abstract：:The Bacillus spo0H gene codes for sigma H, which, as part of the RNA polymerase holoenzyme E sigma H, is responsible for the transcription of several genes which are expressed at the beginning of the sporulation process. In this communication, we examined the regulation of the spo0H gene of Bacillus subtilis by using ...

journal_title：Journal of bacteriology

authors： Weir J,Predich M,Dubnau E,Nair G,Smith I

更新日期：1991-01-01 00:00:00