Inhibitory effect of diphtheria toxin on amino acid incorporation in Escherichia coli cell-free system.

Abstract:

:The mechanism of action of diphtheria toxin in an Escherichia coli cell-free protein-synthesizing system was examined. When the washed ribosomes were incubated with toxin before addition of messenger ribonucleic acid (RNA), peptide syntheses of (14)C-phenylalanine directed by polyuridylic acid or phage R17 RNA were strongly inhibited by a small amount of toxin. Whereas, if the soluble fraction (105,000 x g supernatant fraction) was preincubated with toxin, no effect of toxin occurred either on the induced protein synthesis or on the activity of guanosine triphosphatase even in the presence of nicotinamide adenine dinucleotide. The binding of (3)H-formylmethionyl-transfer RNA to E. coli ribosomes directed by either R17 RNA or trinucleotide AUG was also decreased by toxin. These findings suggest that diphtheria toxin may prevent the binding of messenger RNA by successfully competing with the AUG for ribosomal binding sites. Sucrose-density gradient studies support this concept by showing the decrease in binding of (3)H-labeled R17 RNA to E. coli ribosomes exposed to toxin.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Tsugawa A,Ohsumi Y,Kato I

doi

10.1128/JB.104.1.152-157.1970

subject

Has Abstract

pub_date

1970-10-01 00:00:00

pages

152-7

issue

1

eissn

0021-9193

issn

1098-5530

journal_volume

104

pub_type

杂志文章
  • Cholera toxins: immunogenicity of the rabbit ileal loop toxin and related antigens.

    abstract::A method of assay of immunogenic potency of the cholera gut toxin is described; it is based on the relation of dose of antigen to neutralizing antibody titer produced in the rabbit under defined conditions and allows quantification of immunogenicity as immunogenic units per milligram of protein. Evidence, based on imm...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.2.985-993.1969

    authors: Kaur J,Burrows W,Cercavski L

    更新日期:1969-11-01 00:00:00

  • Repression of the Staphylococcus aureus accessory gene regulator in serum and in vivo.

    abstract::Subgenomic DNA microarrays were employed to evaluate the expression of the accessory gene regulator (agr locus) as well as multiple virulence-associated genes in Staphylococcus aureus. Gene expression was examined during growth of S. aureus in vitro in standard laboratory medium and rabbit serum and in vivo in subcuta...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.4.1095-1101.2002

    authors: Yarwood JM,McCormick JK,Paustian ML,Kapur V,Schlievert PM

    更新日期:2002-02-01 00:00:00

  • Acid- and base-induced proteins during aerobic and anaerobic growth of Escherichia coli revealed by two-dimensional gel electrophoresis.

    abstract::Proteins induced by acid or base, during long-term aerobic or anaerobic growth in complex medium, were identified in Escherichia coli. Two-dimensional gel electrophoresis revealed pH-dependent induction of 18 proteins, nine of which were identified by N-terminal sequencing. At pH 9, tryptophan deaminase (TnaA) was ind...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.7.2209-2216.1999

    authors: Blankenhorn D,Phillips J,Slonczewski JL

    更新日期:1999-04-01 00:00:00

  • EFFECT OF TEMPERATURE ON THE RATE OF GERMINATION IN BACILLUS CEREUS.

    abstract::Knaysi, Georges (Cornell University, Ithaca, N.Y.). Effect of temperature on the rate of germination in Bacillus cereus. J. Bacteriol. 87:619-622. 1964.-By use of the change of the spores from bright to dark, when observed in dark contrast with a phase microscope, as a criterion for the incipience (first stage) of ger...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.3.619-622.1964

    authors: KNAYSI G

    更新日期:1964-03-01 00:00:00

  • Enzyme pattern and aerobic growth of Saccharomyces cerevisiae under various degrees of glucose limitation.

    abstract::The enzyme pattern of Saccharomyces cerevisiae was followed during batch growth and in continuous culture in a synthetic medium limited for glucose under aerobic conditions. Seven enzymes were measured: succinate-cytochrome c oxidoreductase, malate dehydrogenase, nicotinamide adenine dinucleotide-linked glutamate dehy...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.2.479-486.1968

    authors: Beck C,von Meyenburg HK

    更新日期:1968-08-01 00:00:00

  • O-linked glycosylation ensures the normal conformation of the autotransporter adhesin involved in diffuse adherence.

    abstract::The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is one of the few glycosylated proteins found in Escherichia coli. Glycosylation is mediated by a specific heptosyltransferase encoded by the aah gene, but little is known about the role of this modification and the mechanism involved. In this study, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00969-07

    authors: Charbonneau ME,Girard V,Nikolakakis A,Campos M,Berthiaume F,Dumas F,Lépine F,Mourez M

    更新日期:2007-12-01 00:00:00

  • Colicin tolerance and map location of conjugation-deficient mutants.

    abstract::Con minus mutants, conjugation-defective mutants of Escherichia coli K-12, have been shown to be tolerant to colicins K and L. They map at approximately 14 min on the genetic map. The significance of the colicin tolerance of conjugation-defective mutants is discussed. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.1.372-373.1975

    authors: Davies JK,Reeves P

    更新日期:1975-07-01 00:00:00

  • Nuclear and cell division in Bacillus subtilis: dormant nucleoids in stationary-phase cells and their activation.

    abstract::The morphology of nucleoids and mesosomes of Bacillus subtilis in stationary-and lag-phase cultures was studied by making three-dimensional cell reconstructions in plastic of electron micrographs of serial sections. In cells from stationary cultures, the dormant nucleoids are frequently, but not always, spherical and ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.3.1189-1199.1975

    authors: Van Iterson W,Michels PA,Vyth-Dreese F,Aten JA

    更新日期:1975-03-01 00:00:00

  • Structure of the HopA1(21-102)-ShcA chaperone-effector complex of Pseudomonas syringae reveals conservation of a virulence factor binding motif from animal to plant pathogens.

    abstract::Pseudomonas syringae injects numerous bacterial proteins into host plant cells through a type 3 secretion system (T3SS). One of the first such bacterial effectors discovered, HopA1, is a protein that has unknown functions in the host cell but possesses close homologs that trigger the plant hypersensitive response in r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01621-12

    authors: Janjusevic R,Quezada CM,Small J,Stebbins CE

    更新日期:2013-02-01 00:00:00

  • Three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina.

    abstract::The three-dimensional structure of the nickel-containing hydrogenase from Thiocapsa roseopersicina has been determined at a resolution of 2 nm in the plane and 4 nm in the vertical direction by electron microscopy and computerized image processing on microcrystals of the enzyme. The enzyme forms a large ring-shaped co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.8.2576-2580.1991

    authors: Sherman MB,Orlova EV,Smirnova EA,Hovmöller S,Zorin NA

    更新日期:1991-04-01 00:00:00

  • Effective symbiosis between Rhizobium etli and Phaseolus vulgaris requires the alarmone ppGpp.

    abstract::The symbiotic interaction between Rhizobium etli and Phaseolus vulgaris, the common bean plant, ultimately results in the formation of nitrogen-fixing nodules. Many aspects of the intermediate and late stages of this interaction are still poorly understood. The R. etli relA gene was identified through a genome-wide sc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5460-5469.2005

    authors: Moris M,Braeken K,Schoeters E,Verreth C,Beullens S,Vanderleyden J,Michiels J

    更新日期:2005-08-01 00:00:00

  • The RecF pathway of homologous recombination can mediate the initiation of DNA damage-inducible replication of the Escherichia coli chromosome.

    abstract::DNA damage-inducible DNA replication in SOS-induced Escherichia coli cells, termed inducible stable DNA replication (iSDR), has previously been shown to require either the RecBCD or the RecE pathway of homologous recombination for initiation. Here, we demonstrate that recB recC sbcC quadruple mutant cells are capable ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.22.7113-7114.1994

    authors: Asai T,Kogoma T

    更新日期:1994-11-01 00:00:00

  • Genome sequence of the repetitive-sequence-rich Mycoplasma fermentans strain M64.

    abstract::Mycoplasma fermentans is a microorganism commonly found in the genitourinary and respiratory tracts of healthy individuals and AIDS patients. The complete genome of the repetitive-sequence-rich M. fermentans strain M64 is reported here. Comparative genomics analysis revealed dramatic differences in genome size between...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05228-11

    authors: Shu HW,Liu TT,Chan HI,Liu YM,Wu KM,Shu HY,Tsai SF,Hsiao KJ,Hu WS,Ng WV

    更新日期:2011-08-01 00:00:00

  • Reversibility of SOS-associated division inhibition in Escherichia coli.

    abstract::In Escherichia coli the SOS response, induced by DNA-damaging treatments, includes two systems of cell division inhibition, SfiA and SfiC, which are thought to prevent cell division by interacting with the division protein FtsZ. It is shown here that SfiA-mediated division inhibition is readily reversible, even in the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.166.3.733-738.1986

    authors: Maguin E,Lutkenhaus J,D'Ari R

    更新日期:1986-06-01 00:00:00

  • Bacteroides intermedius binds fibrinogen.

    abstract::The binding of Bacteroides intermedius VPI 8944 to human fibrinogen has been characterized. The binding is time dependent, at least partially reversible, saturable, and specific. On an average, a maximum of 3,500 fibrinogen molecules bind per bacterial cell, with a dissociation constant of 1.7 X 10(-11) M. These bacte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.2.623-628.1985

    authors: Lantz MS,Switalski LM,Kornman KS,Höök M

    更新日期:1985-08-01 00:00:00

  • Mutations in ampG or ampD affect peptidoglycan fragment release from Neisseria gonorrhoeae.

    abstract::Neisseria gonorrhoeae releases peptidoglycan fragments during growth. The majority of the fragments released are peptidoglycan monomers, molecules known to increase pathogenesis through the induction of proinflammatory cytokines and responsible for the killing of ciliated epithelial cells. In other gram-negative bacte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01194-07

    authors: Garcia DL,Dillard JP

    更新日期:2008-06-01 00:00:00

  • Method for fingerprinting yeast cell wall mannans.

    abstract::Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1-->2) and (1-->3) linkages between the mannose units, whereas the less stable (1-->6) linkages of the polysaccharide backbone are cleaved. The "fingerprints," obtained by gel filtration of the oligosaccharide mixtures, can be used to dis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.3.1175-1181.1969

    authors: Kocourek J,Ballou CE

    更新日期:1969-12-01 00:00:00

  • A Suppressor Mutation That Creates a Faster and More Robust σE Envelope Stress Response.

    abstract:UNLABELLED:The σE envelope stress response is an essential signal transduction pathway which detects and removes mistargeted outer membrane (OM) β-barrel proteins (OMPs) in the periplasm of Escherichia coli It relies on σE, an alternative sigma factor encoded by the rpoE gene. Here we report a novel mutation, a nucleot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00340-16

    authors: Konovalova A,Schwalm JA,Silhavy TJ

    更新日期:2016-08-11 00:00:00

  • Mutational analysis of the precursor-specific region of Bacillus subtilis sigma E.

    abstract::sigma E is a sporulation-specific sigma factor of Bacillus subtilis that is formed from an inactive precursor protein (pro-sigma E) by the removal of 27 to 29 amino acids from the pro-sigma E amino terminus. By using oligonucleotide-directed mutagenesis, sequential deletions were constructed in the precursor-specific ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.14.4629-4637.1992

    authors: Peters HK 3rd,Carlson HC,Haldenwang WG

    更新日期:1992-07-01 00:00:00

  • Separation of chromosome termini during sporulation of Bacillus subtilis depends on SpoIIIE.

    abstract::Bacillus subtilis undergoes a highly distinctive division during spore formation. It yields two unequal cells, the mother cell and the prespore, and septum formation is completed before the origin-distal 70% of the chromosome has entered the smaller prespore. The mother cell subsequently engulfs the prespore. Two diff...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01949-06

    authors: Bogush M,Xenopoulos P,Piggot PJ

    更新日期:2007-05-01 00:00:00

  • Mutational analysis of flagellum-independent surface spreading of Serratia marcescens 274 on a low-agar medium.

    abstract::In a previous study (J. O'Rear, L. Alberti, and R. M. Harshey, J. Bacteriol. 174:6125-6137, 1992) we reported the isolation of several transposon mutants of Serratia marcescens 274 that were defective either in swarming alone or in both swimming and swarming motility. All the nonflagellate (Fla-) mutants, while defect...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.4.987-991.1995

    authors: Matsuyama T,Bhasin A,Harshey RM

    更新日期:1995-02-01 00:00:00

  • Isolation and characterization of ribosomes and translation initiation factors from the gram-positive soil bacterium Streptomyces lividans.

    abstract::A primer extension inhibition (toeprint) assay was developed using ribosomes and ribosomal subunits from Streptomyces lividans. This assay allowed the study of ribosome binding to streptomycete leaderless and leadered mRNA. Purified 30S subunits were unable to form a ternary complex on aph leaderless mRNA, whereas 70S...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.186.20.6864-6875.2004

    authors: Day JM,Janssen GR

    更新日期:2004-10-01 00:00:00

  • In vivo DNA binding of bacteriophage GA-1 protein p6.

    abstract::Bacteriophage GA-1 infects Bacillus sp. strain G1R and has a linear double-stranded DNA genome with a terminal protein covalently linked to its 5' ends. GA-1 protein p6 is very abundant in infected cells and binds DNA with no sequence specificity. We show here that it binds in vivo to the whole viral genome, as detect...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01047-07

    authors: Alcorlo M,Salas M,Hermoso JM

    更新日期:2007-11-01 00:00:00

  • The cytochrome bc1 complex of Rhodobacter sphaeroides can restore cytochrome c2-independent photosynthetic growth to a Rhodobacter capsulatus mutant lacking cytochrome bc1.

    abstract::Plasmids encoding the structural genes for the Rhodobacter capsulatus and Rhodobacter sphaeroides cytochrome (cyt) bc1 complexes were introduced into strains of R. capsulatus lacking the cyt bc1 complex, with and without cyt c2. The R. capsulatus merodiploids contained higher than wild-type levels of cyt bc1 complex, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.11.6059-6068.1989

    authors: Davidson E,Prince RC,Haith CE,Daldal F

    更新日期:1989-11-01 00:00:00

  • Genomic stability in the archaeae Haloferax volcanii and Haloferax mediterranei.

    abstract::Through hybridization of available probes, we have added nine genes to the macrorestriction map of the Haloferax mediterranei chromosome and five genes to the contig map of Haloferax volcanii. Additionally, we hybridized 17 of the mapped cosmid clones from H. volcanii to the H. mediterranei genome. The resulting 35-po...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.5.1405-1408.1995

    authors: López-García P,St Jean A,Amils R,Charlebois RL

    更新日期:1995-03-01 00:00:00

  • Independent genes for two threonyl-tRNA synthetases in Bacillus subtilis.

    abstract::With the exception of Escherichia coli lysyl-tRNA synthetase, the genes coding for the different aminoacyl-tRNA synthetases in procaryotes are always unique. Here we report on the occurrence and cloning of two genes (thrSv and thrS2), both encoding functional threonyl-tRNA synthetase in Bacillus subtilis. The two prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.8.4593-4602.1990

    authors: Putzer H,Brakhage AA,Grunberg-Manago M

    更新日期:1990-08-01 00:00:00

  • Sequence of the pckA gene of Escherichia coli K-12: relevance to genetic and allosteric regulation and homology of E. coli phosphoenolpyruvate carboxykinase with the enzymes from Trypanosoma brucei and Saccharomyces cerevisiae.

    abstract::The sequence of the pckA gene coding for phosphoenolpyruvate carboxykinase in Escherichia coli K-12 and previous molecular weight determinations indicate that this allosteric enzyme is a monomer of Mr 51,316. The protein is homologous to ATP-dependent phosphoenolpyruvate carboxykinases from Trypanosoma brucei and Sacc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.12.7151-7156.1990

    authors: Medina V,Pontarollo R,Glaeske D,Tabel H,Goldie H

    更新日期:1990-12-01 00:00:00

  • Organization and regulation of an operon that encodes a sporulation-essential sigma factor in Bacillus subtilis.

    abstract::Deletion of sigE, the structural gene for the sporulation-induced RNA polymerase sigma factor, sigma E, prevented endospore formation by Bacillus subtilis. The effects of integration of plasmids into the sigE region of the chromosome and the use of complementation analyses demonstrated that sigE is part of an operon t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.7.3329-3339.1987

    authors: Kenney TJ,Moran CP Jr

    更新日期:1987-07-01 00:00:00

  • Molecular cloning of TOL genes xylB and xylE in Escherichia coli.

    abstract::The xylB and xylE genes in the TOL plasmid of Pseudomonas putida mt-2, which code for benzyl alcohol dehydrogenase and catechol 2,3-oxygenase, respectively, were cloned onto plasmid pBR322 in Escherichia coli for detailed mapping. The xylB gene was mapped in a 2.9-kilobase region within the BamHI BC fragment of pTN2, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.145.3.1137-1143.1981

    authors: Inouye S,Nakazawa A,Nakazawa T

    更新日期:1981-03-01 00:00:00

  • Clusters of charged residues at the C terminus of MotA and N terminus of MotB are important for function of the Escherichia coli flagellar motor.

    abstract::MotA contains a conserved C-terminal cluster of negatively charged residues, and MotB contains a conserved N-terminal cluster of positively charged residues. Charge-altering mutations affecting these residues impair motility but do not diminish Mot protein levels. The motility defects are reversed by second-site mutat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00407-08

    authors: Hosking ER,Manson MD

    更新日期:2008-08-01 00:00:00