Organization and regulation of an operon that encodes a sporulation-essential sigma factor in Bacillus subtilis.

abstract：:Coat-stripped spores suspended in hypertonic solutions and supplied with two essential cations can be converted into viable protoplasts by lysozyme digestion of both cortex and germ cell wall. Calcium ions are necessary to prevent membrane rupture, and magnesium ions are necessary for changes indicative of hydration o...

journal_title：Journal of bacteriology

authors： Fitz-James PC

更新日期：1971-03-01 00:00:00

abstract：:Flavobacterium johnsoniae cells glide rapidly over surfaces by an unknown mechanism. Transposon-induced sprA mutants formed nonspreading colonies on agar, and the cells examined in wet mounts were deficient in attachment to surfaces and were almost completely nonmotile. Exposure of intact cells to proteinase K cleaved...

journal_title：Journal of bacteriology

authors： Nelson SS,Glocka PP,Agarwal S,Grimm DP,McBride MJ

更新日期：2007-10-01 00:00:00

abstract：:We have investigated the mechanism of osmotic stress adaptation (osmoregulation) in Agrobacterium tumefaciens biotype I (salt-tolerant) and biotype II (salt-sensitive) strains. Using natural-abundance 13C nuclear magnetic resonance spectroscopy, we identified all organic solutes that accumulated to significant levels ...

journal_title：Journal of bacteriology

authors： Smith LT,Smith GM,Madkour MA

更新日期：1990-12-01 00:00:00

abstract：:Plasmid pUB110 was previously used as a vector to clone fragments of deoxyribonucleic acid that complement the trpC2 mutation in Bacillus subtilis from endonuclease EcoRI digested B. licheniformis, B. pumilus, and B. subtilis cellular deoxyribonucleic acid. Each of several such trp plasmids was subsequently shown to c...

journal_title：Journal of bacteriology

authors： Keggins KM,Lovett PS,Marrero R,Hoch SO

更新日期：1979-09-01 00:00:00

abstract：:Oxalate catabolism is conducted by phylogenetically diverse organisms, including Methylobacterium extorquens AM1. Here, we investigate the central metabolism of this alphaproteobacterium during growth on oxalate by using proteomics, mutant characterization, and (13)C-labeling experiments. Our results confirm that ener...

journal_title：Journal of bacteriology

authors： Schneider K,Skovran E,Vorholt JA

更新日期：2012-06-01 00:00:00

abstract：:Sinorhizobium meliloti can live as a soil saprophyte and can engage in a nitrogen-fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma (σ) factors interacting wit...

journal_title：Journal of bacteriology

authors： Barnett MJ,Bittner AN,Toman CJ,Oke V,Long SR

更新日期：2012-09-01 00:00:00

abstract：:Evidence of the presence of messenger ribonucleic acid (mRNA) in dormant spores of Bacillus subtilis has been obtained. The bulk RNA from spores was isolated and labeled in vitro with tritiated dimethyl sulfate. The spore RNA hybridized to 2.4 to 3.2% of the B. subtilis genome. The RNA hybridized to both the complemen...

journal_title：Journal of bacteriology

authors： Jeng YH,Doi RH

更新日期：1974-08-01 00:00:00

abstract：:Pseudomonas cepacia mutants deficient in either 6-phosphogluconate (6PGA) dehydratase (Edd-) or 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase (Eda-) failed to utilize glucose or gluconate despite the prominence of of 6-phosphogluconate dehydrogenase (6PGAD) ii this bacterium and the potential for utilizing the pen...

journal_title：Journal of bacteriology

authors： Allenza P,Lessie TG

更新日期：1982-06-01 00:00:00

abstract：:The diversity of regulatory systems encoded by bacteria provides an indication of the variety of stresses and interactions that these organisms encounter in nature. We have been investigating how the plant pathogen Pseudomonas syringae pv. tomato DC3000 responds to iron limitation and have focused on the iron starvati...

journal_title：Journal of bacteriology

authors： Markel E,Maciak C,Butcher BG,Myers CR,Stodghill P,Bao Z,Cartinhour S,Swingle B

更新日期：2011-10-01 00:00:00

abstract：:Satellite bacteriophage P4 requires the products of the late genes of a helper such as P2 in order to grow lytically. The Escherichia coli rpoA109 mutation, which alters the alpha subunit of RNA polymerase, prevents transcription of the late genes of bacteriophage P2. Suppressor mutations that define the P2 ogr gene o...

journal_title：Journal of bacteriology

authors： Halling C,Sunshine MG,Lane KB,Six EW,Calendar R

更新日期：1990-07-01 00:00:00

abstract：:Identification of new genes involved in biofilm formation is needed to understand the molecular basis of strain variation and the pathogenic mechanisms implicated in chronic staphylococcal infections. A biofilm-producing Staphylococcus aureus isolate was used to generate biofilm-negative transposon (Tn917) insertion m...

journal_title：Journal of bacteriology

authors： Cucarella C,Solano C,Valle J,Amorena B,Lasa I,Penadés JR

更新日期：2001-05-01 00:00:00

abstract：:Lipid composition was studied in two strains each of mid-log phase cells of Bacillus thuringiensis, B. larvae, B. popilliae, B. alvei, and B. lentimorbus. Total lipids varied from 2.5 to 3.5% of the cell dry weight of B. thuringiensis to 4.3 to 5.0% of B. popilliae. Phospholipids in the organisms examined ranged from ...

journal_title：Journal of bacteriology

authors： Bulla LA,Bennett GA,Shotwell OL

更新日期：1970-12-01 00:00:00

abstract：:Cell division of Escherichia coli K-12 strain PA3092 was inhibited by the addition of adenosine 3',5'-phosphate (cAMP), and the cellular morphology was changed from rods into filaments. Nucleoids in the filaments were regularly distributed and septum formation was perfectly inhibited. This inhibition of cell division ...

journal_title：Journal of bacteriology

authors： Utsumi R,Tanabe H,Nakamoto Y,Kawamukai M,Sakai H,Himeno M,Komano T,Hirota Y

更新日期：1981-09-01 00:00:00

abstract：:We studied the activity of a debranching enzyme (TreX) from Sulfolobus solfataricus on glycogen-mimic substrates, branched maltotetraosyl-β-cyclodextrin (Glc₄-β-CD), and natural glycogen to better understand substrate transglycosylation and the effect thereof on glycogen debranching in microorganisms. The validation t...

journal_title：Journal of bacteriology

authors： Nguyen DH,Park JT,Shim JH,Tran PL,Oktavina EF,Nguyen TL,Lee SJ,Park CS,Li D,Park SH,Stapleton D,Lee JS,Park KH

更新日期：2014-06-01 00:00:00

abstract：:The essential function of penicillin-binding protein 2 (PBP2) in methicillin-susceptible Staphylococcus aureus RN4220 was clearly established by placing the pbp2 gene under control of the inducible P(spac) promoter; the resulting bacteria were unable to grow in the absence of inducer. In contrast, the deficit in PBP2 ...

journal_title：Journal of bacteriology

authors： Pinho MG,Filipe SR,de Lencastre H,Tomasz A

更新日期：2001-11-01 00:00:00

abstract：:The flaW, flaU, and flaV genes of Salmonella typhimurium LT2 were cloned into pBR322. These genes were mapped on the cloned DNA fragments by restriction endonuclease analysis and construction of the deletion derivatives. Their gene products were identified, by the minicell method, as proteins whose molecular weights w...

journal_title：Journal of bacteriology

authors： Homma M,Kutsukake K,Iino T

更新日期：1985-08-01 00:00:00

abstract：:Two-micrometer deoxyribonucleic acid (DNA), a circular plasmid of Saccharomyces cerevisiae, contains two nontandem repeated sequences which are inverted with respect to one another. These repeated sequences together account for 21% of the molecule length. Restriction endonuclease analysis and electron microscopy demon...

journal_title：Journal of bacteriology

authors： Livingston DM,Klein HL

更新日期：1977-01-01 00:00:00

abstract：:ClpX is a well-characterized bacterial chaperone that plays a role in many processes, including protein turnover and the remodeling of macromolecular complexes. All of these activities require ATP hydrolysis-dependent, ClpX-mediated protein unfolding. Here we used site-directed mutagenesis in combination with genetics...

journal_title：Journal of bacteriology

authors： Haeusser DP,Lee AH,Weart RB,Levin PA

更新日期：2009-03-01 00:00:00

abstract：:Pseudomonas aeruginosa XMG, isolated from soil, utilizes lactate. Here we present a 6.45-Mb assembly of its genome sequence. Besides the lactate utilization mechanism of the strain, the genome sequence may also provide other useful information related to P. aeruginosa, such as identifying genes involved in virulence, ...

journal_title：Journal of bacteriology

authors： Gao C,Hu C,Ma C,Su F,Yu H,Jiang T,Dou P,Wang Y,Qin T,Lv M,Xu P

更新日期：2012-09-01 00:00:00

abstract：:The cis-acting regulatory regions of the tps and ops genes of Myxococcus xanthus were localized by analyzing the expression of fusions of these genes with the lacZ gene. A 201-base-pair (bp) fragment of tps DNa extending 95 bp upstream (-95) from the transcriptional start was sufficient to direct developmentally regul...

journal_title：Journal of bacteriology

authors： Downard JS,Kim SH,Kil KS

更新日期：1988-10-01 00:00:00

abstract：:Growth of a derivative of Escherichia coli K-10 was strongly inhibited by 2 times 10(-4) M L-5(1-amino-2-methylbutyl)-tetrazole (isoleucine tetrazole). Growth inhibition was reversed by isoleucine, threonine, glycyl-L-isoleucine, or glycyl-L-threonine, and, in a valine-resistant mutant, by L-valine. Partial reversal o...

journal_title：Journal of bacteriology

authors： Willshaw GA,Tristram H

更新日期：1975-09-01 00:00:00

abstract：:The pbpB gene product penicillin-binding protein 3 (PBP3) of Escherichia coli is one of the major targets of beta-lactam antibiotics. At the permissive temperature, the temperature-sensitive pbpBr1 mutant, which was obtained after selection for increased resistance to cephalexin, shows a dramatic change in shape which...

journal_title：Journal of bacteriology

authors： Taschner PE,Ypenburg N,Spratt BG,Woldringh CL

更新日期：1988-10-01 00:00:00

abstract：:Cystic fibrosis (CF) patients chronically infected with both Pseudomonas aeruginosa and Staphylococcus aureus have worse health outcomes than patients who are monoinfected with either P. aeruginosa or S. aureus We showed previously that mucoid strains of P. aeruginosa can coexist with S. aureusin vitro due to the tran...

journal_title：Journal of bacteriology

authors： Price CE,Brown DG,Limoli DH,Phelan VV,O'Toole GA

更新日期：2020-03-26 00:00:00

abstract：:The ability to restart broken DNA replication forks is essential across all domains of life. In Escherichia coli, the priA, priB, priC, and dnaT genes encode the replication restart proteins (RRPs) to accomplish this task. PriA plays a critical role in replication restart such that its absence reveals a dramatic pheno...

journal_title：Journal of bacteriology

authors： Leroux M,Jani N,Sandler SJ

更新日期：2017-08-08 00:00:00

abstract：UNLABELLED:FimV is a Pseudomonas aeruginosa inner membrane protein that regulates intracellular cyclic AMP (cAMP) levels-and thus type IV pilus (T4P)-mediated twitching motility and type II secretion (T2S)-by activating the adenylate cyclase CyaB. Its cytoplasmic domain contains three predicted tetratricopeptide repeat...

journal_title：Journal of bacteriology

authors： Buensuceso RN,Nguyen Y,Zhang K,Daniel-Ivad M,Sugiman-Marangos SN,Fleetwood AD,Zhulin IB,Junop MS,Howell PL,Burrows LL

更新日期：2016-07-28 00:00:00

abstract：:Weiss, Emilio (Naval Medical Research Institute, Bethesda, Md.) and Harry R. Dressler. Increased resistance to chloramphenicol in Rickettsia prowazekii with a note on failure to demonstrate genetic interaction among strains. J. Bacteriol. 83:409-414. 1962.-A further effort to produce genetically labeled Rickettsia pro...

journal_title：Journal of bacteriology

authors： WEISS E,DRESSLER HR

更新日期：1962-02-01 00:00:00

abstract：:Molecular phylogenetic surveys have recently revealed an ecologically widespread crenarchaeal group that inhabits cold and temperate terrestrial and marine environments. To date these organisms have resisted isolation in pure culture, and so their phenotypic and genotypic characteristics remain largely unknown. To cha...

journal_title：Journal of bacteriology

authors： Schleper C,DeLong EF,Preston CM,Feldman RA,Wu KY,Swanson RV

更新日期：1998-10-01 00:00:00

abstract：:Ralstonia eutropha JMP134(pJP4) degrades 3-chlorobenzoate (3-CB) by using two not completely isofunctional, pJP4-encoded chlorocatechol degradation gene clusters, tfdC(I)D(I)E(I)F(I) and tfdD(II)C(II)E(II)F(II). Introduction of several copies of each gene cluster into R. eutropha JMP222, which lacks pJP4 and thus accu...

journal_title：Journal of bacteriology

authors： Pérez-Pantoja D,Ledger T,Pieper DH,González B

更新日期：2003-03-01 00:00:00

abstract：:Two chemotactic transducers for inorganic phosphate (P(i)), designated CtpH and CtpL, have been identified in Pseudomonas aeruginosa. The corresponding genes (ctpH and ctpL) were inactivated by inserting kanamycin and tetracycline resistance gene cassettes into the wild-type genes in the P. aeruginosa PAO1 genome. Com...

journal_title：Journal of bacteriology

authors： Wu H,Kato J,Kuroda A,Ikeda T,Takiguchi N,Ohtake H

更新日期：2000-06-01 00:00:00

abstract：:In Neurospora crassa, histidine starvation of histidine mutants resulted in derepression of histidine, tryptophan, and arginine biosynthetic enzymes. The same tripartite derepression occurred in wild-type strain 74A when it was grown in medium supplemented with 3-amino-1,2,4-triazole, an inhibitor of histidine biosynt...

journal_title：Journal of bacteriology

authors： Carsiotis M,Jones RF,Wesseling AC

更新日期：1974-09-01 00:00:00