A combination of sbmA and tolC mutations in Escherichia coli K-12 Tn10-carrying strains results in hypersusceptibility to tetracycline.

Abstract:

:Previously, we demonstrated that Escherichia coli tolC mutations reduce the high-level resistance to tetracycline afforded by the transposon Tn10-encoded TetA pump from resistance at 200 microg/ml to resistance at 40 microg/ml. In this study, we found that the addition of an sbmA mutation to a tolC::Tn10 mutant exacerbates this phenotype: the double mutant did not form colonies, even in the presence of tetracycline at a concentration as low as 5 microg/ml. Inactivation of sbmA alone partially inhibited high-level tetracycline resistance, from resistance at 200 microg/ml to resistance at 120 microg/ml. There thus appears to be an additive effect of the mutations, resulting in almost complete suppression of the phenotypic expression of Tn10 tetracycline resistance.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

de Cristóbal RE,Vincent PA,Salomón RA

doi

10.1128/JB.01844-07

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

1491-4

issue

4

eissn

0021-9193

issn

1098-5530

pii

JB.01844-07

journal_volume

190

pub_type

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