Abstract:
:The gene for an extremely thermostable oligo-1,6-glucosidase (dextrin-6-alpha-D-glucanohydrolase; EC 3.2.1.10) of obligately thermophilic Bacillus thermoglucosidasius KP1006 was cloned within a 4.2-kilobase HindIII-PvuII fragment of DNA by using the plasmid pUC19 as a vector and Escherichia coli C600 as a host. The gene was transcribed, presumably from its own promoter, in E. coli. E. coli with the hybrid plasmid accumulated oligo-1,6-glucosidase mainly in the cytoplasm. The level of enzyme production was comparable to that observed for B. thermoglucosidasius. The enzyme coincided absolutely with the B. thermoglucosidasius enzyme in its molecular weight (60,000), in its electrophoretic behavior on denaturing and nondenaturing polyacrylamide gels, in the temperature dependency of its stability and activity, and in its antigenic determinants.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Watanabe K,Iha H,Ohashi A,Suzuki Ydoi
10.1128/jb.171.2.1219-1222.1989subject
Has Abstractpub_date
1989-02-01 00:00:00pages
1219-22issue
2eissn
0021-9193issn
1098-5530journal_volume
171pub_type
杂志文章abstract::The Streptomyces coelicolor absA two-component system was initially identified through analysis of mutations in the sensor kinase absA1 that caused inhibition of all four antibiotics synthesized by this strain. Previous genetic analysis had suggested that the phosphorylated form of AbsA2 acted as a negative regulator ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.3.794-805.2002
更新日期:2002-02-01 00:00:00
abstract::Production of Bacillus subtilis exoproteases is positively regulated by the DegS-DegU two-component regulatory system and other regulatory factors including DegR and ProB. It was shown that the expression of degR was virtually abolished in a sigD mutant and that the transcriptional initiation site in vivo is preceded ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.1.216-222.1996
更新日期:1996-01-01 00:00:00
abstract::The origin of replication of Bacillus subtilis 168 trp thy dna-1 (temperature-sensitive initiation mutant) was labeled with [3H]thymidine. Analysis of labeled cells by autoradiography revealed that most of the radioactivity was associated with cell pole areas. To label the terminus, cells that had initiated were treat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.3.895-899.1985
更新日期:1985-09-01 00:00:00
abstract::The prfA gene of Listeria monocytogenes was recently reported to be required for expression of hly, which encodes a pore-forming hemolysin essential for pathogenicity (M. Leimeister-Wachter, C. Haffner, E. Domann, W. Goebel, and T. Chakraborty, Proc. Natl. Acad. Sci. USA 87:8336-8340, 1990). We demonstrate here that a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.4.1293-1298.1992
更新日期:1992-02-01 00:00:00
abstract::Congo red binding by virulent A-layer-containing (A+) and avirulent A-layer-deficient (A-) strains of Aeromonas salmonicida was examined. Congo red binding to A+ cells was enhanced by salt and thus hydrophobically driven, but at low Congo red concentrations binding was salt independent. Congo red was bound by A+ cells...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.3.1332-1336.1985
更新日期:1985-12-01 00:00:00
abstract::The maintenance energy coefficient of Desulfovibrio vulgaris was studied by using a chemostat, with Methanosarcina barkeri or sulfate as the electron acceptor; lithium lactate or sodium pyruvate served as the electron donor. The experiments showed that the growth energetics of D. vulgaris or M. barkeri were greatly af...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1260-1264.1983
更新日期:1983-09-01 00:00:00
abstract::An examination of the methanofurans isolated from a wide range of methanogenic bacteria and from Archaeoglobus fulgidus has revealed at least five chromatographically distinct methanofurans. Bacteria from each major genus of methanogenic bacteria have been found to contain a chemically different methanofuran. The natu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4594-4597.1988
更新日期:1988-10-01 00:00:00
abstract::Freeman, Bob A. (University of Chicago, Chicago, Ill.) and Richard Circo. Effect of tetracyclines on the intracellular amino acids of molds. J. Bacteriol. 86:38-44. 1963.-The tetracycline antibiotics were shown to alter the amino acid metabolism of molds whose growth is not markedly affected. Eight molds were grown in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.1.38-44.1963
更新日期:1963-07-01 00:00:00
abstract::The structures of the lipooligosaccharides (LOSs) from Haemophilus ducreyi ITM 5535 and ITM 3147 and a fresh clinical isolate, ACY1, have been investigated. Oligosaccharides were obtained from phenol-water-extracted LOS by mild acid hydrolysis and were studied by methylation analysis, fast atom bombardment and electro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.18.5316-5321.1995
更新日期:1995-09-01 00:00:00
abstract::Several spontaneous cya and crp mutants of Escherichia coli have been selected as clones simultaneously resistant to phage lambda and nalidixic acid and characterized. Both cya and crp mutants have been found to grow as cocci with increased doubling times. They have increased resistance to some mutagens (methylmethane...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.2.545-555.1976
更新日期:1976-02-01 00:00:00
abstract::Bacillus subtilis DegS-DegU belongs to a bacterial two-component system that controls many processes, including the production of exocellular proteases and competence development. It was found that when the glutamine synthetase gene glnA, which is involved in nitrogen regulation, was disrupted, the expression of the r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00368-08
更新日期:2008-08-01 00:00:00
abstract::The heat-stable enterotoxin (ST) produced by enterotoxigenic Escherichia coli is an extracellular peptide toxin that evokes watery diarrhea in the host. Two types of STs, STI and STII, have been found. Both STs are synthesized as precursor proteins and are then converted to the active forms with intramolecular disulfi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00853-08
更新日期:2008-12-01 00:00:00
abstract::Peptide inhibitors of phage lambda site-specific recombination were previously isolated by screening synthetic combinatorial peptide libraries. These inhibitors cause the accumulation of complexes between the recombinase and the Holliday junction intermediate of several highly divergent tyrosine recombinases. Peptide ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01559-08
更新日期:2009-04-01 00:00:00
abstract::Mutants of Rhizobium leguminosarum which are deficient in normal polysaccharide production have been isolated and characterized. A correlation between diminished production of extracellular polysaccharide and reduced infection and nodulation efficiency has been observed. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.141.3.1454-1456.1980
更新日期:1980-03-01 00:00:00
abstract::The gal3 mutation of Saccharomyces, which is associated with an impairment in the utilization of galactose, has been shown to be pleiotropic, causing similar impairments in the utilization of melibiose and maltose. Milibiose utilization and alpha-galactosidase production are directly controlled by the galactose regula...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.33-41.1976
更新日期:1976-01-01 00:00:00
abstract::We have cloned three copies of a repeated DNA segment from Borrelia burgdorferi sensu stricto strain B31, present on both circular and linear plasmids of this and other B. burgdorferi sensu lato strains. The DNA sequences are characterized by a highly homologous segment containing two open reading frames (ORFs), ORF-A...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.8.2287-2298.1996
更新日期:1996-04-01 00:00:00
abstract::An apparatus has been developed in which bacterial growth can be measured very precisely over short intervals of time. Its precision is presented and used to assess the constancy of growth in batch culture. Under certain conditions, i.e., Luria broth or 0.2% glucose-M9 medium at very low cell densities, the specific g...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.3.969-975.1978
更新日期:1978-12-01 00:00:00
abstract::In previous work with membranes of Bacillus subtilis, the succinate dehydrogenase complex was isolated by immunoprecipitation of Triton X-100-solubilized membranes. The complex included a polypeptide with an apparent molecular weight of 19,000, probably attributable to apocytochrome. This paper reports the further cha...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.144.3.933-940.1980
更新日期:1980-12-01 00:00:00
abstract::The alpha-aminoadipate pathway for the biosynthesis of lysine is present only in fungi and euglena. Until now, this unique metabolic pathway has never been investigated in the opportunistic fungal pathogens Candida albicans, Cryptococcus neoformans, and Aspergillus fumigatus. Five of the eight enzymes (homocitrate syn...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.22.7379-7384.1992
更新日期:1992-11-01 00:00:00
abstract::Covalently closed circular deoxyribonucleic acid (DNA) was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types. In each of the two strains circular DNA of about 20 times 10-6 daltons could be detected, amounting to about 1.5% of the total cellular DNA. The possible...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.416-421.1975
更新日期:1975-02-01 00:00:00
abstract::A fatty acid Delta(9)-desaturase mutant of yeast was analyzed to establish the specificity of the fatty acid structural requirement. Several double-bond-containing and substituted fatty acids were tested. It was concluded that the presence of a Delta(9)cis double bond is necessary for growth. The need for a specific c...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.1.160-165.1970
更新日期:1970-01-01 00:00:00
abstract::Goldberg, Herbert S. (University of Missouri, Columbia), Ella M. Barnes, and Anthony B. Charles. Unusual Bacteroides-like organism. J. Bacteriol. 87:737-742. 1964.-An organism is described which appears to be a new species of gram-negative, anaerobic, nonsporulating rod. It was isolated from poultry caeca at levels of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.3.737-742.1964
更新日期:1964-03-01 00:00:00
abstract::Farrar, W. Edmund, Jr. (Walter Reed Army Institute of Research, Washington, D.C.), Thomas H. Kent, and Van B. Elliott. Lethal gram-negative bacterial superinfection in guinea pigs given bacitracin. J. Bacteriol. 92:496-501. 1966.-Oral administration of a single dose of bacitracin (either 2,000 or 10,000 units) was let...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.2.496-501.1966
更新日期:1966-08-01 00:00:00
abstract::Oligopeptidase B (OpdB) is a serine peptidase broadly distributed among unicellular eukaryotes, gram-negative bacteria, and spirochetes which has emerged as an important virulence factor and potential therapeutic target in infectious diseases. We report here the cloning and expression of the opdB homologue from Salmon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.12.3329-3337.2002
更新日期:2002-06-01 00:00:00
abstract::The obligate intracellular pathogen Chlamydia trachomatis expresses a type III secretion system (T3SS) which has the potential to contribute significantly to pathogenesis. Based on a demonstrated role of type III secretion (T3S)-specific chaperones in the secretion of antihost proteins by gram-negative pathogens, we i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.18.6466-6478.2005
更新日期:2005-09-01 00:00:00
abstract::We isolated an unusual mutant in which the arabinose operon carried an araC lesion that led to a dominant fucose resistance while retaining recessive constitutivity. All previously characterized C(c) mutations have been recessive for both fucose resistance and constitutivity. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.2.711-713.1973
更新日期:1973-08-01 00:00:00
abstract::The essential function of penicillin-binding protein 2 (PBP2) in methicillin-susceptible Staphylococcus aureus RN4220 was clearly established by placing the pbp2 gene under control of the inducible P(spac) promoter; the resulting bacteria were unable to grow in the absence of inducer. In contrast, the deficit in PBP2 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.22.6525-6531.2001
更新日期:2001-11-01 00:00:00
abstract::Escherichia coli sigma70-dependent promoters have typically been characterized as either -10/-35 promoters, which have good matches to both the canonical -10 and the -35 sequences or as extended -10 promoters (TGn/-10 promoters), which have the TGn motif and an excellent match to the -10 consensus sequence. We report ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00853-06
更新日期:2006-12-01 00:00:00
abstract::Cultures of a stable L-phase variant of Streptococcus faecium F24 produced and retained peptidoglycan precursors intracellularly over the entire growth cycle in a chemically defined medium. The identity of the most abundant precursor, UDP N-acetylmuramyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine (UDP-MurNAc-penta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.3.900-910.1978
更新日期:1978-09-01 00:00:00
abstract::Acinetobacter baumannii is a Gram-negative bacterium causing nosocomial infections worldwide. To gain quick insight into the molecular basis of biofilm formation in A. baumannii, we determined the complete genome sequence of A. baumannii strain 1656-2, which forms sturdy biofilm and is resistant to multiple drugs. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.06109-11
更新日期:2011-11-01 00:00:00