Regulation of the Streptomyces coelicolor calcium-dependent antibiotic by absA, encoding a cluster-linked two-component system.

Abstract:

:The Streptomyces coelicolor absA two-component system was initially identified through analysis of mutations in the sensor kinase absA1 that caused inhibition of all four antibiotics synthesized by this strain. Previous genetic analysis had suggested that the phosphorylated form of AbsA2 acted as a negative regulator of antibiotic biosynthesis in S. coelicolor (T. B. Anderson, P. Brian, and W. C. Champness, Mol. Microbiol. 39:553-566, 2001). Genomic sequence data subsequently provided by the Sanger Centre (Cambridge, United Kingdom) revealed that absA was located within the gene cluster for production of one of the four antibiotics, calcium-dependent antibiotic (CDA). In this paper we have identified numerous transcriptional start sites within the CDA cluster and have shown that the original antibiotic-negative mutants used to identify absA exhibit a stronger negative regulation of promoters upstream of the proposed CDA biosynthetic genes than of promoters in the clusters responsible for production of actinorhodin and undecylprodigiosin. The same antibiotic-negative mutants also showed an increase in transcription from a promoter divergent to that of absA, upstream of a putative ABC transporter, in addition to an increase in transcription of absA itself. Interestingly, the negative regulation of the biosynthetic transcripts did not appear to be mediated by transcriptional regulation of cdaR (a gene encoding a homolog of the pathway-specific regulators of the act and red clusters) or by any other recognizable transcriptional regulator associated with the cluster. The role of absA in regulating the expression of the diverse antibiotic biosynthesis clusters in the genome is discussed in light of its location in the cda cluster.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Ryding NJ,Anderson TB,Champness WC

doi

10.1128/jb.184.3.794-805.2002

keywords:

subject

Has Abstract

pub_date

2002-02-01 00:00:00

pages

794-805

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

184

pub_type

杂志文章
  • Isolation of pigmentation mutants of the green filamentous photosynthetic bacterium Chloroflexus aurantiacus.

    abstract::Mutants deficient in the production of bacteriochlorophyll c (Bchl c) and one mutant lacking colored carotenoids were isolated from the filamentous gliding bacterium Chloroflexus aurantiacus. Mutagenesis was achieved by using UV radiation or N-methyl-N'-nitro-N-nitrosoguanidine. Several clones were isolated that were ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.1.222-227.1984

    authors: Pierson BK,Keith LM,Leovy JG

    更新日期:1984-07-01 00:00:00

  • Ultrastructure of paracrystals of a lipoprotein from the outer membrane of Escherichia coli.

    abstract::The highly purified lipoprotein of the outer membrane of Escherichia coli forms paracrystals. The ultrastructures of these paracrystals were examined by electron microscopy. The needle-shaped paracrystals show several different band patterns, depending on conditions of paracrystallization. Models are presented to expl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.1.564-571.1976

    authors: DeMartini M,Inouye S,Inouye M

    更新日期:1976-07-01 00:00:00

  • Scanning electron microscopy of intact colonies of microorganisms.

    abstract::Colonies of S. mutans OMZ61, Streptococcus sp. D182, Staphylococcus aureus Oxford NCTC 6571, and Candida albicans type A, MRL 3153 were grown on various media. Cubes of agar bearing two to three colonies were excised and processed for scanning electron microscopy. The characteristic shape of the colonies was seen when...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.2.902-909.1970

    authors: Whittaker DK,Drucker DB

    更新日期:1970-11-01 00:00:00

  • Transfection of group H streptococci.

    abstract::Transfection of streptococci is reported here for the first time. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.113.3.1505-1506.1973

    authors: Parsons CL,Cole RM

    更新日期:1973-03-01 00:00:00

  • Insertional inactivation of dsbA produces sensitivity to cadmium and zinc in Escherichia coli.

    abstract::In a search for genes that produce hypersensitivity to cadmium salts in Escherichia coli, random transposon mutagenesis with TnphoA was used. One of the mutant strains obtained was sensitive to Cd2+ and Zn2+. Sequence analysis showed that the TnphoA insertion was located in the dsbA gene coding for a periplasmic prote...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.8.2769-2771.1997

    authors: Rensing C,Mitra B,Rosen BP

    更新日期:1997-04-01 00:00:00

  • Role of plant root exudate and Sym plasmid-localized nodulation genes in the synthesis by Rhizobium leguminosarum of Tsr factor, which causes thick and short roots on common vetch.

    abstract::In a previous paper it was shown that cocultivation of Rhizobium leguminosarum with the plant Vicia sativa subsp. nigra on solid medium causes a changed mode of growth of the plant roots, resulting in thick and short roots (Tsr). The Sym plasmid present in the bacterium appeared to be essential for causing Tsr (A. A. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.2.517-522.1986

    authors: Van Brussel AA,Zaat SA,Cremers HC,Wijffelman CA,Pees E,Tak T,Lugtenberg BJ

    更新日期:1986-02-01 00:00:00

  • Escherichia coli RNA polymerase recognition of a sigma70-dependent promoter requiring a -35 DNA element and an extended -10 TGn motif.

    abstract::Escherichia coli sigma70-dependent promoters have typically been characterized as either -10/-35 promoters, which have good matches to both the canonical -10 and the -35 sequences or as extended -10 promoters (TGn/-10 promoters), which have the TGn motif and an excellent match to the -10 consensus sequence. We report ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00853-06

    authors: Hook-Barnard I,Johnson XB,Hinton DM

    更新日期:2006-12-01 00:00:00

  • Purification and biochemical characterization of tellurite-reducing activities from Thermus thermophilus HB8.

    abstract::Cell-free extracts of Thermus thermophilus HB8 catalyze the in vitro, NADH-dependent reduction of potassium tellurite (K2TeO3). Three different protein fractions with tellurite-reducing activities were identified. Two exhibited high molecular weight and were composed of at least two different polypeptides. The protein...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3269-3273.1988

    authors: Chiong M,González E,Barra R,Vásquez C

    更新日期:1988-07-01 00:00:00

  • Specificity of the heme requirement for growth of Bacteroides ruminicola.

    abstract::Caldwell, D. R. (U.S. Department of Agriculture, Beltsville, Md.), D. C. White, M. P. Bryant, and R. N. Doetsch. Specificity of the heme requirement for growth of Bacteroides ruminicola. J. Bacteriol. 90:1645-1654. 1965.-Previous studies suggested that most strains of Bacteroides ruminicola subsp. ruminicola require h...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.6.1645-1654.1965

    authors: Caldwell DR,White DC,Bryant MP,Doetsch RN

    更新日期:1965-12-01 00:00:00

  • HK022 Nun Requires Arginine-Rich Motif Residues Distinct from λ N.

    abstract:UNLABELLED:Bacteriophage λ N protein binds boxB RNA hairpins in the nut (N utilization) sites of immediate early λ transcripts and interacts with host factors to suppress transcriptional termination at downstream terminators. In opposition to λ N, the Nun protein of HK022 binds the boxBs of coinfecting λ transcripts, i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00466-15

    authors: Tawk CS,Ghattas IR,Smith CA

    更新日期:2015-11-01 00:00:00

  • Characterization and DNA sequence of the mobilization region of pLV22a from Bacteroides fragilis.

    abstract::A 4.2-kb plasmid (pLV22a) native to Bacteroides fragilis LV22 became fused to a transfer-deficient Bacteroides spp.-Escherichia coli shuttle vector by an inverse transposition event, resulting in a transferrable phenotype. The transfer phenotype was attributable to pLV22a, which was also capable of mobilization within...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.15.4466-4473.1995

    authors: Novicki TJ,Hecht DW

    更新日期:1995-08-01 00:00:00

  • Functional complementation between bacterial MDR-like export systems: colicin V, alpha-hemolysin, and Erwinia protease.

    abstract::The antibacterial protein Colicin V (ColV) is secreted from gram-negative bacteria by a signal sequence-independent pathway. The proteins that mediate the export of ColV share sequence similarities with components from other signal sequence-independent export systems such as those for alpha-hemolysin (Hly) and Erwinia...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.23.7549-7556.1991

    authors: Fath MJ,Skvirsky RC,Kolter R

    更新日期:1991-12-01 00:00:00

  • O-linked glycosylation ensures the normal conformation of the autotransporter adhesin involved in diffuse adherence.

    abstract::The Escherichia coli adhesin involved in diffuse adherence (AIDA-I) is one of the few glycosylated proteins found in Escherichia coli. Glycosylation is mediated by a specific heptosyltransferase encoded by the aah gene, but little is known about the role of this modification and the mechanism involved. In this study, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00969-07

    authors: Charbonneau ME,Girard V,Nikolakakis A,Campos M,Berthiaume F,Dumas F,Lépine F,Mourez M

    更新日期:2007-12-01 00:00:00

  • New aerobic benzoate oxidation pathway via benzoyl-coenzyme A and 3-hydroxybenzoyl-coenzyme A in a denitrifying Pseudomonas sp.

    abstract::A denitrifying Pseudomonas sp. is able to oxidize aromatic compounds compounds completely to CO2, both aerobically and anaerobically. It is shown that benzoate is aerobically oxidized by a new degradation pathway via benzoyl-coenzyme A (CoA) and 3-hydroxybenzoyl-CoA. The organism grew aerobically with benzoate, 3-hydr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.15.4851-4858.1993

    authors: Altenschmidt U,Oswald B,Steiner E,Herrmann H,Fuchs G

    更新日期:1993-08-01 00:00:00

  • Molecular characterization of "plasmid-free" antibiotic-resistant Haemophilus influenzae.

    abstract::We examined 14 multiresistant and 8 ampicillin- or tetracycline-resistant Haemophilus influenzae isolates and 4 ampicillin-resistant H. parainfluenzae isolates for plasmid deoxyribonucleic acid. Sixteen strains carried plasmids. Both "plasmid-free" and plasmid-carrying isolates transferred the antibiotic resistance by...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.1.476-479.1980

    authors: Roberts MC,Smith AL

    更新日期:1980-10-01 00:00:00

  • Energetic efficiency of Escherichia coli: effects of mutations in components of the aerobic respiratory chain.

    abstract::The aerobic respiratory chain of Escherichia coli can function with either of two different membrane-bound NADH dehydrogenases (NDH-1 and NDH-2) and with either of two ubiquinol oxidases (bd-type and bo-type). The amounts of each of these enzymes present in the E. coli membrane depend on growth conditions in general a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.10.3020-3025.1993

    authors: Calhoun MW,Oden KL,Gennis RB,de Mattos MJ,Neijssel OM

    更新日期:1993-05-01 00:00:00

  • Role of lipid intermediate(s) in the synthesis of serogroup B Neisseria meningitidis capsular polysaccharide.

    abstract::Neisseria meningitidis serogroup B strain M986 was examined for the involvement of lipid intermediate(s) participating in the biosynthesis of the sialic acid capsular polysaccharide. The addition of exogenous undecaprenyl phosphate, phosphatidylethanolamine, or phosphatidylglycerol to particulate membranes, in the pre...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.3.861-867.1985

    authors: Masson L,Holbein BE

    更新日期:1985-03-01 00:00:00

  • Ultrastructure of Azotobacter vinelandii.

    abstract::Vegetative cells and cysts of Azotobacter vinelandii 12837 were prepared for electron microscopy by several methods assumed to preserve structural details destroyed by techniques previously reported in the literature. Examination of large numbers of cells and cysts by these methods revealed four structural details not...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.2.933-939.1970

    authors: Vela GR,Cagle GD,Holmgren PR

    更新日期:1970-11-01 00:00:00

  • Mutagenesis of specificity and toxicity regions of a Bacillus thuringiensis protoxin gene.

    abstract::Two different 30-nucleotide regions of the cryIAc insecticidal protoxin gene from Bacillus thuringiensis were randomly mutagenized. One region was within one of seven amphipathic helices believed to be important for the formation of ion channels. There was no loss of toxicity for three test insects by any of 27 mutant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.14.4059-4065.1995

    authors: Aronson AI,Wu D,Zhang C

    更新日期:1995-07-01 00:00:00

  • SufU is an essential iron-sulfur cluster scaffold protein in Bacillus subtilis.

    abstract::Bacteria use three distinct systems for iron-sulfur (Fe/S) cluster biogenesis: the ISC, SUF, and NIF machineries. The ISC and SUF systems are widely distributed, and many bacteria possess both of them. In Escherichia coli, ISC is the major and constitutive system, whereas SUF is induced under iron starvation and/or ox...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01536-09

    authors: Albrecht AG,Netz DJ,Miethke M,Pierik AJ,Burghaus O,Peuckert F,Lill R,Marahiel MA

    更新日期:2010-03-01 00:00:00

  • Characterization of an operon encoding an NADP-reducing hydrogenase in Desulfovibrio fructosovorans.

    abstract::A genomic DNA fragment from Desulfovibrio fructosovorans, which strongly hybridized with the hydAB genes from Desulfovibrio vulgaris Hildenborough, was cloned and sequenced. This fragment was found to contain four genes, named hndA, hndB, hndC, and hndD. Analysis of the sequence homologies indicated that HndA shows 29...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.10.2628-2636.1995

    authors: Malki S,Saimmaime I,De Luca G,Rousset M,Dermoun Z,Belaich JP

    更新日期:1995-05-01 00:00:00

  • The Pseudomonas aeruginosa devB/SOL homolog, pgl, is a member of the hex regulon and encodes 6-phosphogluconolactonase.

    abstract::A cyclic version of the Entner-Doudoroff pathway is used by Pseudomonas aeruginosa to metabolize carbohydrates. Genes encoding the enzymes that catabolize intracellular glucose to pyruvate and glyceraldehyde 3-phosphate are coordinately regulated, clustered at 39 min on the chromosome, and collectively form the hex re...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.14.3934-3941.2000

    authors: Hager PW,Calfee MW,Phibbs PV

    更新日期:2000-07-01 00:00:00

  • Cloning and nucleotide sequence analysis of the colH gene from Clostridium histolyticum encoding a collagenase and a gelatinase.

    abstract::The colH gene encoding a collagenase was cloned from Clostridium histolyticum JCM 1403. Nucleotide sequencing showed a major open reading frame encoding a 116-kDa protein of 1,021 amino acid residues. The deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, HEX...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.21.6489-6496.1994

    authors: Yoshihara K,Matsushita O,Minami J,Okabe A

    更新日期:1994-11-01 00:00:00

  • Respiratory metabolism of a "petite negative"yeast Schizosaccharomyces pombe 972h-.

    abstract::The respiratory metabolism of Schizosaccharomyces pombe 972h(-), a fission, haplontic, "petite negative" yeast, was studied. Glucose and glycerol are good growth substrates and are oxidized under appropriate conditions. l-Lactate, ethanol, malate, and succinate are oxidized but are poor substrates for growth. d-Lactat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.1.473-481.1970

    authors: Heslot H,Goffeau A,Louis C

    更新日期:1970-10-01 00:00:00

  • Spontaneous erythromycin resistance mutation in a 23S rRNA gene, rrlA, of the extreme thermophile Thermus thermophilus IB-21.

    abstract::Spontaneous, erythromycin-resistant mutants of Thermus thermophilus IB-21 were isolated and found to carry the mutation A2058G in one of two 23S rRNA operons. The heterozygosity of these mutants indicates that A2058G confers a dominant or codominant phenotype in this organism. This mutation provides a valuable tool fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.14.4382-4385.2001

    authors: Gregory ST,Cate JH,Dahlberg AE

    更新日期:2001-07-01 00:00:00

  • Control of EpsE, the phosphoglycosyltransferase initiating exopolysaccharide synthesis in Streptococcus thermophilus, by EpsD tyrosine kinase.

    abstract::We studied the roles of Streptococcus thermophilus phosphogalactosyltransferase (EpsE) (the priming enzyme), tyrosine kinase (EpsD), phosphatase (EpsB), and a membrane-associated protein with no known biochemical function (EpsC) in exopolysaccharide (EPS) synthesis. These proteins are well-conserved among bacteria and...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01122-06

    authors: Minic Z,Marie C,Delorme C,Faurie JM,Mercier G,Ehrlich D,Renault P

    更新日期:2007-02-01 00:00:00

  • Replication control mutations of plasmid R6-5 and their effects on interactions of the RNA-I control element with its target.

    abstract::Nine high copy number mutations of plasmid R6-5, representing five phenotypically distinct groups, have been identified by DNA sequencing. In each mutant plasmid examined, a single nucleotide change was found. The effects of the mutations on possible gene products, and DNA-RNA secondary structure, were analyzed and co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.1.429-436.1983

    authors: Brady G,Frey J,Danbara H,Timmis KN

    更新日期:1983-04-01 00:00:00

  • Evidence for horizontal gene transfer in evolution of elongation factor Tu in enterococci.

    abstract::The elongation factor Tu, encoded by tuf genes, is a GTP binding protein that plays a central role in protein synthesis. One to three tuf genes per genome are present, depending on the bacterial species. Most low-G+C-content gram-positive bacteria carry only one tuf gene. We have designed degenerate PCR primers derive...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.24.6913-6920.2000

    authors: Ke D,Boissinot M,Huletsky A,Picard FJ,Frenette J,Ouellette M,Roy PH,Bergeron MG

    更新日期:2000-12-01 00:00:00

  • Genetic regulation of glycogen biosynthesis in Escherichia coli: in vitro effects of cyclic AMP and guanosine 5'-diphosphate 3'-diphosphate and analysis of in vivo transcripts.

    abstract::Glycogen accumulation in Escherichia coli is inversely related to the growth rate and occurs most actively when cells enter the stationary phase. The levels of the three biosynthetic enzymes undergo corresponding changes under these conditions, suggesting that genetic control of enzyme biosynthesis may account for at ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2773-2782.1989

    authors: Romeo T,Preiss J

    更新日期:1989-05-01 00:00:00

  • Molecular characterization of the PhoP-PhoQ two-component system in Escherichia coli K-12: identification of extracellular Mg2+-responsive promoters.

    abstract::We identified Mg2+-responsive promoters of the phoPQ, mgtA, and mgrB genes of Escherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites were also determined, which allowed us to find a (T/G)GTTTA direct repe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.17.5516-5520.1999

    authors: Kato A,Tanabe H,Utsumi R

    更新日期:1999-09-01 00:00:00