Structural studies of lipooligosaccharides from Haemophilus ducreyi ITM 5535, ITM 3147, and a fresh clinical isolate, ACY1: evidence for intrastrain heterogeneity with the production of mutually exclusive sialylated or elongated glycoforms.

Abstract:

:The structures of the lipooligosaccharides (LOSs) from Haemophilus ducreyi ITM 5535 and ITM 3147 and a fresh clinical isolate, ACY1, have been investigated. Oligosaccharides were obtained from phenol-water-extracted LOS by mild acid hydrolysis and were studied by methylation analysis, fast atom bombardment and electrospray ionization mass spectrometry, and nuclear magnetic resonance spectroscopy. The major oligosaccharide obtained from all strains was a nonasaccharide with the structure beta-D-Galp-(1-->4)-beta-D-GlcNAcp-(1-->3)-beta-D-Galp-(1-->4)-D-a lpha-D-Hepp- (1-->6)-beta-D-Glcp-(1-->[L-alpha-D-Hepp-(1-->2)-L-alpha-D-Hepp - (1-->3)]4)-L-alpha-D-Hepp-Kdo (Kdo stands for 3-deoxy-D-manno-octulosonic acid) and is thus identical to that identified as the major oligosaccharide in H. ducreyi ITM 2665 (E. K. H. Schweda, A. C. Sundström, L. M. Eriksson, J.A. Jonasson, and A. A. Lindberg, J. Biol. Chem. 269:12040-12048, 1994). Electrospray ionization mass spectrometry on O-deacylated LOS from H. ducreyi ITM 5535 obtained after treatment with anhydrous hydrazine gave evidence for the presence of a sialylated major compound, Neu5Ac alpha(2-->3)-beta-D-Galp-(1-->4)-beta-D-GlcNAcp-(1-->3)-beta-D-Gal p- (1-->4)-D-alpha-D-Hepp-(1-->6)-beta-D-Glcp-(1-->[L-alpha-D-Hepp -(1-->2)-L- alpha-D-Hepp-(1-->3)]4)-L-alpha-D-Hepp-Kdo(P)-O-deacylated lipid A (Neu5Ac stands for N-acetylneuraminic acid). However, an even larger oligosaccharide could be isolated from all strains as a minor component, viz., the undecasaccharide beta-D-Galp-(1-->4)-beta-D-GlcNAcp-(1-->3)-beta-d-Galp-(1-->4)-beta-D-glcNAcp-(1-->3)-beta-D-Galp-(1-->4)-D-alpha-D-Hepp-(1-->6)-beta-D-Glcp-(1-->[L-alpha-D-Hepp-(1-->2)-L-alpha-D-Hepp-(1-->3)]4-L-alpha-D-Hepp-Kdo, which represents an N-acetyl lactosamine disaccharide unit elongation of the LOS outer core. No Sialylation of this latter minor component undecasaccharide was detected.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Schweda EK,Jonasson JA,Jansson PE

doi

10.1128/jb.177.18.5316-5321.1995

subject

Has Abstract

pub_date

1995-09-01 00:00:00

pages

5316-21

issue

18

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

杂志文章
  • Environmental role of pathogenic traits in Vibrio cholerae.

    abstract::Vibrio cholerae is a natural inhabitant of aquatic ecosystems. Some strains of V. cholerae can colonize the human host and cause cholera, a profuse watery diarrhea. The major pathogenicity factors and virulence regulators of V. cholerae are either encoded in mobile genetic elements acquired in the environment (e.g. pa...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00795-17

    authors: Sakib SN,Reddi G,Almagro-Moreno S

    更新日期:2018-03-26 00:00:00

  • Are thiosulfate and trithionate intermediates in dissimilatory sulfate reduction?

    abstract::The fate of 35-S during anaerobic metabolism of [35-S]sulfate, [35-S]thiosulfate, and [35-S]sulfate plus unlabeled thiosulfate by washed cell suspensions of Desulfovibrio spp, and of [35-S]thiosulfate by growing D. desulfuricans was examined. The results appear to be inconsistent with the hypothesis that thiosulfate i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.123.1.36-40.1975

    authors: Chambers LA,Trudinger PA

    更新日期:1975-07-01 00:00:00

  • The FtsH protein accumulates at the septum of Bacillus subtilis during cell division and sporulation.

    abstract::The ftsH gene encodes an ATP- and Zn(2+)-dependent metalloprotease which is anchored to the cytoplasmic membrane via two transmembrane segments in such a way that the very short amino- and the long carboxy termini are exposed to the cytoplasm. Deletion of the ftsH gene in Bacillus subtilis results in a pleiotropic phe...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.13.3870-3873.2000

    authors: Wehrl W,Niederweis M,Schumann W

    更新日期:2000-07-01 00:00:00

  • Sociality in Escherichia coli: Enterochelin Is a Private Good at Low Cell Density and Can Be Shared at High Cell Density.

    abstract:UNLABELLED:Many bacteria produce secreted iron chelators called siderophores, which can be shared among cells with specific siderophore uptake systems regardless of whether the cell produces siderophores. Sharing secreted products allows freeloading, where individuals use resources without bearing the cost of productio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02596-14

    authors: Scholz RL,Greenberg EP

    更新日期:2015-07-01 00:00:00

  • Kinetics of labeling of the S-adenosylmethionine pool of Saccharomyces cerevisiae.

    abstract::A method has been developed for studying the specific activity of the pool of S-adenosylmethionine in yeast. The pool reaches half-maximal specific activity within 30 s after the addition of [methyl-3H]methionine. After addition of an excess of nonradioactive methionine, the specific activity of S-adenosylmethionine i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.3.887-891.1976

    authors: Warner JR,Morgan SA,Shulman RW

    更新日期:1976-03-01 00:00:00

  • Role of the heat shock response in stability of mRNA in Escherichia coli K-12.

    abstract::The heat shock response in Escherichia coli involves extensive induction of the heat shock proteins, with the concomitant suppression of the synthesis of the non-heat shock proteins. While the induction of the heat shock proteins has been shown to occur primarily at the transcriptional level, the suppression of non-he...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.3.743-748.1992

    authors: Henry MD,Yancey SD,Kushner SR

    更新日期:1992-02-01 00:00:00

  • Toxicity of Pasteurella tularensis killed by ionizing radiation.

    abstract::Approximately 2 x 10(11) viable Pasteurella tularensis cells per ml, contained in suspensions, were killed by exposure to 10(6) r of gamma-radiation. When injected intraperitoneally into mice, the irradiated suspensions initially contained about 10 ld(50) per ml, and immunized mice against challenge with fully virulen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Landay ME,Wright GG,Pulliam JD,Finegold MJ

    更新日期:1968-09-01 00:00:00

  • Transport of p-nitrophenyl-alpha-maltoside by the maltose transport system of Escherichia coli and its subsequent hydrolysis by a cytoplasmic alpha-maltosidase.

    abstract::In wild-type Escherichia coli the activity of the maltose transport system is dependent on a periplasmic maltose-binding protein. It has been possible, however, to isolate mutants in which transport activity is mediated by the membrane components of the system and is no longer dependent on the periplasmic binding prot...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.165.3.918-922.1986

    authors: Reyes M,Treptow NA,Shuman HA

    更新日期:1986-03-01 00:00:00

  • Regulation of ribonucleoside diphosphate reductase synthesis in Escherichia coli: increased enzyme synthesis as a result of inhibition of deoxyribonucleic acid synthesis.

    abstract::Inhibition of deoxyribonucleic acid (DNA) synthesis in Escherichia coli by chemical inhibitors or by shifting cultures of temperature-sensitive elongation (dnaE and dnaB) or initiation (dnaA) mutants to nonpermissive conditions led to greatly increased synthesis of the enzyme ribonucleoside diphosphate reductase, whic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.107-113.1977

    authors: Filpula D,Fuchs JA

    更新日期:1977-04-01 00:00:00

  • Inhibition of a thymine-deficient mutant of Escherichia coli by 5-substituted uracils.

    abstract::Shapira, Jacob (Consolidated Veterans Administration Hospital, Little Rock, Ark.), Lois Lowden, and Ralph Hale. Inhibition of a thymine-deficient mutant of Escherichia coli by 5-substituted uracils. J. Bacteriol. 83:919-923. 1962.-Small inocula of well-washed cells of a thymine-requiring mutant of Escherichia coli wer...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.83.4.919-923.1962

    authors: SHAPIRA J,LOWDEN L,HALE R

    更新日期:1962-04-01 00:00:00

  • DETECTION OF ENTEROPATHOGENIC ESCHERICHIA COLI IN FECAL CULTURES BY USE OF A MODIFIED FLUORESCENT-ANTIBODY TECHNIQUE.

    abstract::Martin, Alice J. (Nassau County Department of Health, Hempstead, N.Y.), and Margaret O'Brien. Detection of enteropathogenic Escherichia coli in fecal cultures by use of a modified fluorescent-antibody technique. J. Bacteriol. 89:570-573. 1965.-The application of the fluorescent-antibody (FA) technique to cultures of f...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.3.570-573.1965

    authors: MARTIN AJ,O'BRIEN M

    更新日期:1965-03-01 00:00:00

  • Functional and structural analysis of HicA3-HicB3, a novel toxin-antitoxin system of Yersinia pestis.

    abstract::The mechanisms involved in the virulence of Yersinia pestis, the plague pathogen, are not fully understood. In previous research, we found that a Y. pestis mutant lacking the HicB3 (YPO3369) putative orphan antitoxin was attenuated for virulence in a murine model of bubonic plague. Toxin-antitoxin systems (TASs) are w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01932-14

    authors: Bibi-Triki S,Li de la Sierra-Gallay I,Lazar N,Leroy A,Van Tilbeurgh H,Sebbane F,Pradel E

    更新日期:2014-11-01 00:00:00

  • Characterization of the primary immunity region of the Escherichia coli linear plasmid prophage N15.

    abstract::N15 is the only bacteriophage of Escherichia coli known to lysogenize as a linear plasmid. Clear-plaque mutations lie in at least two regions of the 46-kb genome. We have cloned, sequenced, and characterized the primary immunity region, immB. This region contains a gene, cB, whose product shows homology to lambdoid ph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.10.2902-2910.1996

    authors: Lobocka MB,Svarchevsky AN,Rybchin VN,Yarmolinsky MB

    更新日期:1996-05-01 00:00:00

  • Transcriptional analysis of pqqD and study of the regulation of pyrroloquinoline quinone biosynthesis in Methylobacterium extorquens AM1.

    abstract::Methanol dehydrogenase, the enzyme that oxidizes methanol to formaldehyde in gram-negative methylotrophs, contains the prosthetic group pyrroloquinoline quinone (PQQ). To begin to analyze how the synthesis of PQQ is coordinated with the production of other methanol dehydrogenase components, the transcription of one of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.1.206-211.1995

    authors: Ramamoorthi R,Lidstrom ME

    更新日期:1995-01-01 00:00:00

  • A Bacillus cereus cytolytic determinant, cereolysin AB, which comprises the phospholipase C and sphingomyelinase genes: nucleotide sequence and genetic linkage.

    abstract::A cloned cytolytic determinant from the genome of Bacillus cereus GP-4 has been characterized at the molecular level. Nucleotide sequence determination revealed the presence of two open reading frames. Both open reading frames were found by deletion and complementation analysis to be necessary for expression of the he...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.2.744-753.1989

    authors: Gilmore MS,Cruz-Rodz AL,Leimeister-Wächter M,Kreft J,Goebel W

    更新日期:1989-02-01 00:00:00

  • Expression of the Bacillus subtilis acsA gene: position and sequence context affect cre-mediated carbon catabolite repression.

    abstract::In Bacillus subtilis, carbon catabolite repression (CCR) of many genes is mediated at cis-acting carbon repression elements (cre) by the catabolite repressor protein CcpA. Mutations in transcription-repair coupling factor (mfd) partially relieve CCR at cre sites located downstream of transcriptional start sites by abo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.24.6649-6654.1998

    authors: Zalieckas JM,Wray LV Jr,Fisher SH

    更新日期:1998-12-01 00:00:00

  • Mapping of genes involved in macromolecular synthesis on the chromosome of Streptomyces coelicolor A3(2).

    abstract::The genes for the beta, beta', and seven sigma factor subunits of RNA polymerase, for elongation factors EF-Tu1 and EF-Tu3, and for six rRNA operons were mapped on the combined genetic and physical map of the Streptomyces coelicolor chromosome. Like the previously mapped tRNA genes, the RNA polymerase and rRNA genes m...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.2.473-476.1995

    authors: van Wezel GP,Buttner MJ,Vijgenboom E,Bosch L,Hopwood DA,Kieser HM

    更新日期:1995-01-01 00:00:00

  • Initial cloning and sequencing of hydHG, an operon homologous to ntrBC and regulating the labile hydrogenase activity in Escherichia coli K-12.

    abstract::To isolate genes from Escherichia coli which regulate the labile hydrogenase activity, a plasmid library was used to transform hydL mutants lacking the labile hydrogenase. A single type of gene, designated hydG, was isolated. This gene also partially restored the hydrogenase activity in hydF mutants (which are defecti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.8.4448-4456.1989

    authors: Stoker K,Reijnders WN,Oltmann LF,Stouthamer AH

    更新日期:1989-08-01 00:00:00

  • Tumor induction by Agrobacterium tumefaciens: specific transfer of bacterial deoxyribonucleic acid to plant tissue.

    abstract::When Agrobacterium tumefaciens cells grown in the presence of tritiated thymidine to label specifically the bacterial deoxyribonucleic acid (DNA) are incubated with carrot root tissue for short periods of time, an appreciable fraction of the label becomes firmly associated with the root tissue. Such association is not...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.108.3.973-979.1971

    authors: Yajko DM,Hegeman GD

    更新日期:1971-12-01 00:00:00

  • The pbgPE operon in Photorhabdus luminescens is required for pathogenicity and symbiosis.

    abstract::Photorhabdus is a genus of gram-negative Enterobacteriaceae that is pathogenic to insect larvae while also maintaining a mutualistic relationship with nematodes from the family Heterorhabditis, where the bacteria occupy the gut of the infective juvenile (IJ) stage of the nematode. In this study we describe the identif...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.1.77-84.2005

    authors: Bennett HP,Clarke DJ

    更新日期:2005-01-01 00:00:00

  • The ABC-type multidrug resistance transporter LmrCD is responsible for an extrusion-based mechanism of bile acid resistance in Lactococcus lactis.

    abstract::Upon prolonged exposure to cholate and other toxic compounds, Lactococcus lactis develops a multidrug resistance phenotype that has been attributed to an elevated expression of the heterodimeric ABC-type multidrug transporter LmrCD. To investigate the molecular basis of bile acid resistance in L. lactis and to evaluat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00485-08

    authors: Zaidi AH,Bakkes PJ,Lubelski J,Agustiandari H,Kuipers OP,Driessen AJ

    更新日期:2008-11-01 00:00:00

  • Secondary and tertiary responses of the induced bactericidin from the West Indian spiny lobster, Panulirus argus.

    abstract::West Indian spiny lobsters, Panulirus argus, synthesized a hemolymph bactericidin after being injected with killed suspensions of gram-negative bacillus EMB-1 isolated from the normal gut of this lobster. To study differences between the primary response and secondary response, animals were given a primary antigen inj...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.98.3.943-946.1969

    authors: Evans EE,Weinheimer PF,Painter B,Acton RT,Evans ML

    更新日期:1969-06-01 00:00:00

  • The proton motive force generated in Leuconostoc oenos by L-malate fermentation.

    abstract::In cells of Leuconostoc oenos, the fermentation of L-malic acid generates both a transmembrane pH gradient, inside alkaline, and an electrical potential gradient, inside negative. In resting cells, the proton motive force ranged from -170 mV to -88 mV between pH 3.1 and 5.6 in the presence Of L-malate. Membrane potent...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.11.3127-3132.1996

    authors: Salema M,Lolkema JS,San Romão MV,Lourero Dias MC

    更新日期:1996-06-01 00:00:00

  • F factor-mediated immunity to lethal zygosis in Escherichia coli K-12.

    abstract::Recipient (F(-)) cells of Escherichia coli are sensitive to an excess of Hfr donor cells. This phenomenon of lethal zygosis is associated with conjugation and is observed as a continuous fall in F(-) viable cells during liquid mating, or as inhibition of F(-) growth on solid media. One class of survivors, which arose ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.1.100-106.1974

    authors: Skurray RA,Reeves P

    更新日期:1974-01-01 00:00:00

  • Refining the Application of Microbial Lipids as Tracers of Staphylococcus aureus Growth Rates in Cystic Fibrosis Sputum.

    abstract::Chronic lung infections in cystic fibrosis (CF) could be treated more effectively if the effects of antimicrobials on pathogens in situ were known. Here, we compared changes in the microbial community composition and pathogen growth rates in longitudinal studies of seven pediatric CF patients undergoing intravenous an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00365-18

    authors: Neubauer C,Kasi AS,Grahl N,Sessions AL,Kopf SH,Kato R,Hogan DA,Newman DK

    更新日期:2018-11-26 00:00:00

  • The MscS and MscL families of mechanosensitive channels act as microbial emergency release valves.

    abstract::Single-celled organisms must survive exposure to environmental extremes. Perhaps one of the most variable and potentially life-threatening changes that can occur is that of a rapid and acute decrease in external osmolarity. This easily translates into several atmospheres of additional pressure that can build up within...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章,评审

    doi:10.1128/JB.00576-12

    authors: Booth IR,Blount P

    更新日期:2012-09-01 00:00:00

  • Characterization of the PduS cobalamin reductase of Salmonella enterica and its role in the Pdu microcompartment.

    abstract::Salmonella enterica degrades 1,2-propanediol (1,2-PD) in a coenzyme B12 (adenosylcobalamin, AdoCbl)-dependent fashion. Salmonella obtains AdoCbl by assimilation of complex precursors, such as vitamin B12 and hydroxocobalamin. Assimilation of these compounds requires reduction of their central cobalt atom from Co3+ to ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00575-10

    authors: Cheng S,Bobik TA

    更新日期:2010-10-01 00:00:00

  • Initiation of chromosome replication in bacteria: analysis of an inhibitor control model.

    abstract::This article contains an analysis of a version of the well-known inhibitor-dilution model for the control of initiation of chromosome replication in bacteria. According to this model, an unstable inhibitor interacts with an initiation primer in a hit-and-destroy fashion to prevent successful initiation; both constitue...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.11.5231-5240.1987

    authors: Margalit H,Grover NB

    更新日期:1987-11-01 00:00:00

  • Inducible gamma-butyrobetaine-degrading enzymes in Pseudomonas species AK 1.

    abstract::Activities of gamma-butyrobetaine hydroxylase and carnitine dehydrogenase were low in cells of Pseudomonas sp. AK 1 grown in the absence of their respective substrates. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.3.1094-1095.1970

    authors: Lindstedt G,Lindstedt S,Midtvedt T,Tofft M

    更新日期:1970-03-01 00:00:00

  • The yjeB (nsrR) gene of Escherichia coli encodes a nitric oxide-sensitive transcriptional regulator.

    abstract::Microarray studies of the Escherichia coli response to nitric oxide and nitrosative stress have suggested that additional transcriptional regulators of this response remain to be characterized. We identify here the product of the yjeB gene as a negative regulator of the transcription of the ytfE, hmpA and ygbA genes, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.3.874-881.2006

    authors: Bodenmiller DM,Spiro S

    更新日期:2006-02-01 00:00:00