Abstract:
:Quorum sensing is involved in the regulation of multicellular behavior through communication via small molecules. Given the high number and diversity of the gastrointestinal microbiota, it is postulated that members of this community communicate to coordinate a variety of adaptive processes. AI-2 is suggested to be a universal bacterial signaling molecule synthesized by the LuxS enzyme, which forms an integral part of the activated methyl cycle. We have previously reported that the well-documented probiotic strain Lactobacillus rhamnosus GG, a human isolate, produces AI-2-like molecules. In this study, we identified the luxS homologue of L. rhamnosus GG. luxS seems to be located in an operon with a yxjH gene encoding a putative cobalamin-independent methionine synthase. In silico analysis revealed a methionine-specific T box in the leader sequence of the putative yxjH-luxS operon. However, transcriptional analysis showed that luxS is expressed mainly as a monocistronic transcript. Construction of a luxS knockout mutant confirmed that the luxS gene is responsible for AI-2 production in L. rhamnosus GG. However, this mutation also resulted in pleiotropic effects on the growth of this fastidious strain. Cysteine, pantothenate, folic acid, and biotin could partially complement growth, suggesting a central metabolic role for luxS in L. rhamnosus GG. Interestingly, the luxS mutant also showed a defect in monospecies biofilm formation. Experiments with chemically synthesized (S)-4,5-dihydroxy-2,3-pentanedione, coculture with the wild type, and nutritional complementation suggested that the main cause of this defect has a metabolic nature. Moreover, our data indicate that suppressor mutations are likely to occur in luxS mutants of L. rhamnosus GG. Therefore, results of luxS-related studies should be carefully interpreted.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Lebeer S,De Keersmaecker SC,Verhoeven TL,Fadda AA,Marchal K,Vanderleyden Jdoi
10.1128/JB.01394-06subject
Has Abstractpub_date
2007-02-01 00:00:00pages
860-71issue
3eissn
0021-9193issn
1098-5530pii
JB.01394-06journal_volume
189pub_type
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journal_title:Journal of bacteriology
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doi:10.1128/JB.108.3.973-979.1971
更新日期:1971-12-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.9.2842-2851.1991
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/jb.178.19.5797-5802.1996
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更新日期:1980-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3420-3426.1989
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pub_type: 杂志文章
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更新日期:1982-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.93.1.278-285.1967
更新日期:1967-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.122-124.1967
更新日期:1967-01-01 00:00:00
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doi:10.1128/JB.110.3.803-808.1972
更新日期:1972-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.1.260-268.1981
更新日期:1981-04-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:1976-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.6.3440-3448.1989
更新日期:1989-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2007-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.19.5697-5705.2003
更新日期:2003-10-01 00:00:00
abstract::Acclimation to high salt concentrations involves concerted changes in gene expression. For the majority of salt-regulated genes, the mechanism underlying the induction process is not known. The gene ggpS (sll1566), which encodes the glucosylglycerol-phosphate synthase responsible for the synthesis of the compatible so...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2010-09-01 00:00:00