Abstract:
:Berg, Gerald (Robert A. Taft Sanitary Engineering Center, Cincinnati, Ohio), Eugene K. Harris, Shih L. Chang, and Kenneth A. Busch. Quantitation of viruses by the plaque technique. J. Bacteriol. 85:691-700. 1963.-This paper presents the results of a study on overcrowding as it occurred with several strains of enteroviruses on monkey kidney cell layers. Each cell sheet area was 4,500 mm(2). Dispersion analysis of all plaque counts in the study revealed that the percentage error, in the absence of overcrowding, fluctuated within a range of 7 to 36% about a mean value of about 17%, which is close to what is expected from purely statistical variation in sampling. Overcrowding with Mahoney virus, a mixture of particles producing either rapidly or slowly expanding plaques, resulted primarily from the obscuring effect produced as rapidly expanding plaques obliterated infected foci produced by virus particles responsible for slow-forming plaques. Overcrowding occurred at plaque levels in excess of 35 to 40 per cell sheet. Overcrowding with Mahoney LP virus, a strain derived from a rapidly expanding Mahoney plaque which produced uniformly expanding plaques, did not occur until counts in excess of 60 to 70 per cell sheet were reached. Plaque obscuring was again responsible. Apparent overcrowding with Coxsackie A9 virus, when plaques were not permanently marked from the first day of counting, resulted from coalescence at levels above about 40 to 50 plaques per cell sheet. When plaques were permanently marked, overcrowding resulted from plaque obscuring which did not occur until levels in excess of about 120 per cell sheet were reached.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
BERG G,HARRIS EK,CHANG SL,BUSCH KAdoi
10.1128/JB.85.3.691-700.1963keywords:
subject
Has Abstractpub_date
1963-03-01 00:00:00pages
691-700eissn
0021-9193issn
1098-5530journal_volume
85pub_type
杂志文章abstract::The Saccharomyces cerevisiae mating hormone a-factor has been difficult to isolate reproducibly in sufficient yields by methods using ion-exchange chromatography, probably because of its pronounced hydrophobicity. In this work, a hydrophobic adsorbent (Amberlite XAD-2), in an insoluble bead from, was used to isolate l...
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doi:10.1128/JB.151.3.1153-1161.1982
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abstract::Examination of the ilvF locus at 54 min on the Escherichia coli K-12 chromosome revealed that it is a cryptic gene for expression of a valine-resistant acetohydroxy acid synthase (acetolactate synthase; EC 4.1.3.18) distinct from previously reported isozymes. A spontaneous mutation, ilvF663, yielded IlvF+ enzyme activ...
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journal_title:Journal of bacteriology
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doi:10.1128/JB.90.6.1645-1654.1965
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pub_type: 杂志文章
doi:10.1128/jb.170.7.2904-2913.1988
更新日期:1988-07-01 00:00:00
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更新日期:2005-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:2016-05-13 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.129.2.763-769.1977
更新日期:1977-02-01 00:00:00
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doi:10.1128/JB.127.1.76-83.1976
更新日期:1976-07-01 00:00:00
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更新日期:1978-03-01 00:00:00
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更新日期:1976-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1984-06-01 00:00:00