Defective gamma subunit of ATP synthase (F1F0) from Escherichia coli leads to resistance to aminoglycoside antibiotics.

abstract：:Form I ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) of the Calvin-Benson-Bassham cycle may be divided into two broad phylogenetic groups, referred to as red-like and green-like, based on deduced large subunit amino acid sequences. Unlike the form I enzyme from the closely related organism Rhodobacter spha...

journal_title：Journal of bacteriology

authors： Horken KM,Tabita FR

更新日期：1999-07-01 00:00:00

abstract：:A Pseudomonas strain produced an enzyme capable of deacylating 7 beta-(4-carboxybutanamido)cephalosporanic acid to 7-aminocephalosporanic acid in response to glutaric acid. The gene for the enzyme was cloned within the PstI site of pBR325 as a 7.35-kilobase-pair DNA segment from a mutant of this strain whose enzyme is...

journal_title：Journal of bacteriology

authors： Matsuda A,Komatsu KI

更新日期：1985-09-01 00:00:00

abstract：:Rhodobacter capsulatus mutants unable to fix nitrogen were isolated by random transposon Tn5 mutagenesis. The Tn5 insertion sites of 30 Nif- mutants were mapped within three unlinked chromosomal regions designated A, B, and C. The majority of Tn5 insertions (21 mutants) map within nif region A, characterized by two Cl...

journal_title：Journal of bacteriology

authors： Klipp W,Masepohl B,Pühler A

更新日期：1988-02-01 00:00:00

abstract：:Cells of Candida albicans WO-1 switch frequently and reversibly between two colony-forming phenotypes, white and opaque. In the white form, budding cells appear similar to those of most other strains of C. albicans, but in the opaque form, budding cells are larger, are bean shaped, and possess pimples on the wall. The...

journal_title：Journal of bacteriology

authors： Anderson J,Mihalik R,Soll DR

更新日期：1990-01-01 00:00:00

abstract：:Flagellar phase variation of Salmonella is a phenomenon where two flagellin genes, fliC (phase 1) and fljB (phase 2), are expressed alternately. This is controlled by the inversion of a DNA segment containing the promoter for the fljB gene. The fljB gene constitutes an operon with the fljA gene, which encodes a negati...

journal_title：Journal of bacteriology

authors： Yamamoto S,Kutsukake K

更新日期：2006-02-01 00:00:00

abstract：:The alkaline phosphatases of 29 strains of bacteria assigned by various authors to the genera Aerobacter, Klebsiella and Enterobacter were compared by the micro-complement fixation technique. On the basis of phosphatase resemblance, we recommend that all strains hitherto assigned to Aerobacter aerogenes and Enterobact...

journal_title：Journal of bacteriology

authors： Steffen DL,Cocks GT,Wilson AC

更新日期：1972-06-01 00:00:00

abstract：:PcsB is a protein of unknown function that plays a critical role in cell division in Streptococcus pneumoniae and other ovococcus species of Streptococcus. We constructed isogenic sets of mutants expressing different amounts of PcsB in laboratory strain R6 and virulent serotype 2 strain D39 to evaluate its cellular ro...

journal_title：Journal of bacteriology

authors： Barendt SM,Land AD,Sham LT,Ng WL,Tsui HC,Arnold RJ,Winkler ME

更新日期：2009-05-01 00:00:00

abstract：:The DNA sequence of part of the gnd (6-phosphogluconate dehydrogenase) gene was determined for eight wild strains of Escherichia coli and for Salmonella typhimurium. Since a region of the trp (tryptophan) operon and the phoA (alkaline phosphatase) gene have been sequenced from the same strains, the gene trees for thes...

journal_title：Journal of bacteriology

authors： Dykhuizen DE,Green L

更新日期：1991-11-01 00:00:00

abstract：:Acinetobacter baumannii is a Gram-negative bacterium causing nosocomial infections worldwide. To gain quick insight into the molecular basis of biofilm formation in A. baumannii, we determined the complete genome sequence of A. baumannii strain 1656-2, which forms sturdy biofilm and is resistant to multiple drugs. ...

journal_title：Journal of bacteriology

authors： Park JY,Kim S,Kim SM,Cha SH,Lim SK,Kim J

更新日期：2011-11-01 00:00:00

abstract：:Cells of Escherichia coli take up vitamin B(12) (cyano-cobalamin [CN-Cbl]) and iron chelates by use of sequential active transport processes. Transport of CN-Cbl across the outer membrane and its accumulation in the periplasm is mediated by the TonB-dependent transporter BtuB. Transport across the cytoplasmic membrane...

journal_title：Journal of bacteriology

authors： Cadieux N,Bradbeer C,Reeger-Schneider E,Köster W,Mohanty AK,Wiener MC,Kadner RJ

更新日期：2002-02-01 00:00:00

abstract：:Variations of the membrane filter technique for deoxyribonucleic acid (DNA) hybridizations were studied with respect to Streptomyces species. At the temperatures required for specific hybridization of DNA with the high melting temperature (T(m)) characteristic of Streptomyces, large amounts (up to 97%) of filter-bound...

journal_title：Journal of bacteriology

authors： Okanishi M,Gregory KF

更新日期：1970-12-01 00:00:00

abstract：:In Escherichia coli, T:G mismatches in specific contexts are corrected by a very short patch (VSP) repair system. Previous studies have shown that the product of gene vsr mediates correction of T:G to C:G in the 5'CTAGG/3'GGTCC context and in some related contexts. Amber mutations that arose in CAG sequences in gene c...

journal_title：Journal of bacteriology

authors： Lieb M,Rehmat S

更新日期：1995-02-01 00:00:00

abstract：:Escherichia coli sigma70-dependent promoters have typically been characterized as either -10/-35 promoters, which have good matches to both the canonical -10 and the -35 sequences or as extended -10 promoters (TGn/-10 promoters), which have the TGn motif and an excellent match to the -10 consensus sequence. We report ...

journal_title：Journal of bacteriology

authors： Hook-Barnard I,Johnson XB,Hinton DM

更新日期：2006-12-01 00:00:00

abstract：:Dehalococcoides species are key players in the anaerobic transformation of halogenated solvents at contaminated sites. Here, we analyze isotopologue distributions in amino acid pools from peptides of Dehalococcoides strain CBDB1 after incubation with (13)C-labeled acetate or bicarbonate as a carbon source. The resulti...

journal_title：Journal of bacteriology

authors： Marco-Urrea E,Seifert J,von Bergen M,Adrian L

更新日期：2012-08-01 00:00:00

abstract：:The ability to form type 1 fimbriae (Fim(+)) was readily transduced to 159 out of 161 wild-type motile Fim(-) FIRN strains of Salmonella typhimurium with phage P22 propagated on a Fim(+) donor strain. Fim(+) clones were isolated from about 35% of tests after the fimbriate bacteria in the transduction mixture had been ...

journal_title：Journal of bacteriology

authors： Old DC,Duguid JP

更新日期：1971-09-01 00:00:00

abstract：:Guanine is transported into germinated conidia of Neurospora crassa by the general purine base transport system. Guanine uptake is inhibited by adenine and hypoxanthine but not xanthine. Guanine phosphoribosyltransferase (GPRTase) activity was demonstrated in cell extracts of wild-type germinated conidia. The Km for g...

journal_title：Journal of bacteriology

authors： Magill CW,Sabina RL,Garber TL,Magill JM

更新日期：1982-03-01 00:00:00

abstract：:The interaction of Streptococcus pyogenes (group A streptococcus [GAS]) with its human host requires several surface proteins. In this study, we isolated mutations in a gene required for the surface localization of protein F by transposon mutagenesis of the M6 strain JRS4. This gene (srtA) encodes a protein homologous...

journal_title：Journal of bacteriology

authors： Barnett TC,Scott JR

更新日期：2002-04-01 00:00:00

abstract：:Previous studies have indicated that a single enzyme, "kynureninase," catalyzes the reactions of l-kynurenine to anthranilate and l-3-hydroxykynurenine to 3-hydroxyanthranilate in Neurospora crassa and in other organisms. The present report describes separate enzymes which catalyze these reactions in N. crassa. The fi...

journal_title：Journal of bacteriology

authors： Gaertner FH,Cole KW,Welch GR

更新日期：1971-11-01 00:00:00

abstract：:A gene which complements a paralyzed flagellar mutant of Rhodobacter sphaeroides was sequenced. The derived protein sequence has similarity to MotB. R. sphaeroides MotB lacks the C-terminal peptidoglycan-binding motif of other MotB proteins. This divergence of sequence may reflect the unusual, unidirectional, stop-sta...

journal_title：Journal of bacteriology

authors： Shah DS,Armitage JP,Sockett RE

更新日期：1995-05-01 00:00:00

abstract：:Bacteroides thetaiotaomicron, a gram-negative obligate anaerobe, appears to utilize starch by first binding the polymer to its surface and then translocating it into the periplasmic space. Several genes that encode enzymes or outer membrane proteins involved in starch utilization have been identified. These have been ...

journal_title：Journal of bacteriology

authors： D'Elia JN,Salyers AA

更新日期：1996-12-01 00:00:00

abstract：:We identified Mg2+-responsive promoters of the phoPQ, mgtA, and mgrB genes of Escherichia coli K-12 by S1 nuclease analysis. Expression of these genes was induced by magnesium limitation and depended on PhoP and PhoQ. The transcription start sites were also determined, which allowed us to find a (T/G)GTTTA direct repe...

journal_title：Journal of bacteriology

authors： Kato A,Tanabe H,Utsumi R

更新日期：1999-09-01 00:00:00

abstract：:Azotobacter vinelandii presents a differentiation process leading to the formation of desiccation-resistant cysts. Alginate, the exopolysaccharide produced by this bacterium, has been postulated to have a role in cyst formation. Here, we report the cloning and characterization of the A. vinelandii gene coding for the ...

journal_title：Journal of bacteriology

authors： Campos M,Martínez-Salazar JM,Lloret L,Moreno S,Núñez C,Espín G,Soberón-Chávez G

更新日期：1996-04-01 00:00:00

abstract：:The lic3 locus of Haemophilus influenzae consists of four open reading frames. The derived amino acid sequences of orf2 and orf4 exhibit homology to Escherichia coli GalE and AdK, respectively. The functions of orf1 and orf3 remain unknown. orf1 contains multiple tandem repeats of the tetrameric DNA sequence CAAT near...

journal_title：Journal of bacteriology

authors： Szabo M,Maskell D,Butler P,Love J,Moxon R

更新日期：1992-11-01 00:00:00

abstract：:Two new mutants of Rhodobacter sphaeroides deficient in sulfolipid accumulation were isolated by directly screening mutagenized cell lines for polar lipid composition by thin-layer chromatography of lipid extracts. A genomic clone which complemented the mutations in these two lines, but not the previously described su...

journal_title：Journal of bacteriology

authors： Benning C,Somerville CR

更新日期：1992-10-01 00:00:00

abstract：:The gltA gene, encoding Sinorhizobium meliloti 104A14 citrate synthase, was isolated by complementing an Escherichia coli gltA mutant. The S. meliloti gltA gene was mutated by inserting a kanamycin resistance gene and then using homologous recombination to replace the wild-type gltA with the gltA::kan allele. The resu...

journal_title：Journal of bacteriology

authors： Mortimer MW,McDermott TR,York GM,Walker GC,Kahn ML

更新日期：1999-12-01 00:00:00

abstract：:A temperature-sensitive Schizosaccharomyces pombe mutant, cdc16-116, has been isolated which undergoes uncontrolled septation during its cell division cycle. The mutant accumulates two types of cells after 3 h of growth at the restrictive temperature: (i) type I cells (85% of the population), which complete nuclear di...

journal_title：Journal of bacteriology

authors： Minet M,Nurse P,Thuriaux P,Mitchison JM

更新日期：1979-01-01 00:00:00

abstract：:A 3.3-kilobase PvuII fragment carrying the PHR1 gene of Saccharomyces cerevisiae has been cloned into an Escherichia coli expression vector and introduced into E. coli strains deficient in DNA photolyase. Complementation of the E. coli phr-1 mutation was observed, strongly suggesting that the yeast PHR1 gene encodes a...

journal_title：Journal of bacteriology

authors： Sancar GB

更新日期：1985-02-01 00:00:00

abstract：:Escherichia coli BS-12 uvrA lon is hypersensitive to ultraviolet light. On minimal agar plates at densities in excess of about 10(7) bacteria per plate, as few as one or two photoreversible pyrimidine dimers in the entire genome are sufficient to cause inhibition of cell division. Most of the resulting filaments are u...

journal_title：Journal of bacteriology

authors： Bridges BA,Mottershead RP,Green MH

更新日期：1977-05-01 00:00:00

abstract：:Common histidine-to-aspartate (His-to-Asp) phosphorelay signaling systems involve three types of signaling components: a sensor His kinase, a response regulator, and a histidine-containing phosphotransfer (HPt) protein. In the fission yeast Schizosaccharomyces pombe, two response regulators, Mcs4 and Prr1, have been i...

journal_title：Journal of bacteriology

authors： Aoyama K,Mitsubayashi Y,Aiba H,Mizuno T

更新日期：2000-09-01 00:00:00

abstract：:The response of Salmonella typhimurium to various forms of environmental stress was examined by using O'Farrell two-dimensional gel electrophoresis. Polypeptides (a total of 110) which quantitatively increased during various starvations, anaerobiosis, or heat shock were identified and cataloged in reference to a stand...

journal_title：Journal of bacteriology

authors： Spector MP,Aliabadi Z,Gonzalez T,Foster JW

更新日期：1986-10-01 00:00:00