Spy1, a histidine-containing phosphotransfer signaling protein, regulates the fission yeast cell cycle through the Mcs4 response regulator.

Abstract:

:Common histidine-to-aspartate (His-to-Asp) phosphorelay signaling systems involve three types of signaling components: a sensor His kinase, a response regulator, and a histidine-containing phosphotransfer (HPt) protein. In the fission yeast Schizosaccharomyces pombe, two response regulators, Mcs4 and Prr1, have been identified recently, and it was shown that they are involved in the signal transduction implicated in stress responses. Furthermore, Mcs4 appears to be involved in mitotic cell-cycle control. However, neither the HPt phosphotransmitter nor His kinase has been characterized in S. pombe. In this study, we identified a gene encoding an HPt phosphotransmitter, named Spy1 (S. pombe YPD1-like protein). The spy1(+) gene showed an ability to complement a mutational lesion of the Saccharomyces cerevisiae YPD1 gene, which is involved in an osmosensing signal transduction. The result from yeast two-hybrid analysis indicated that Spy1 interacts with Mcs4. To gain insight into the function of Spy1, a series of genetic analyses were conducted. The results provided evidence that Spy1, together with Mcs4, plays a role in regulation of the G(2)/M cell cycle progression. Spy1-deficient cells appear to be precocious in the entry to M phase. In the proposed model, Spy1 modulates Mcs4 in a negative manner, presumably through a direct His-to-Asp phosphorelay, operating upstream of the Sty1 mitogen-activated protein kinase cascade.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Aoyama K,Mitsubayashi Y,Aiba H,Mizuno T

doi

10.1128/jb.182.17.4868-4874.2000

keywords:

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

4868-74

issue

17

eissn

0021-9193

issn

1098-5530

journal_volume

182

pub_type

杂志文章
  • The requirement for pneumococcal MreC and MreD is relieved by inactivation of the gene encoding PBP1a.

    abstract::MreC and MreD, along with the actin homologue MreB, are required to maintain the shape of rod-shaped bacteria. The depletion of MreCD in rod-shaped bacteria leads to the formation of spherical cells and the accumulation of suppressor mutations. Ovococcus bacteria, such as Streptococcus pneumoniae, lack MreB homologues...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05245-11

    authors: Land AD,Winkler ME

    更新日期:2011-08-01 00:00:00

  • Use of polymyxin B, levallorphan, and tetracaine to isolate novel envelope mutants of Escherichia coli.

    abstract::Mutants of Escherichia coli were isolated by their resistance to the bacteriocidal effects of the membrane-active drugs polymyxin B, levallorphan, and tetracaine. The mutants were examined for additional changes in cellular physiology evoked by the lesions; many polymyxin-resistant strains had a concomitant increased ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.127.2.961-972.1976

    authors: Dame JB,Shapiro BM

    更新日期:1976-08-01 00:00:00

  • The pbgPE operon in Photorhabdus luminescens is required for pathogenicity and symbiosis.

    abstract::Photorhabdus is a genus of gram-negative Enterobacteriaceae that is pathogenic to insect larvae while also maintaining a mutualistic relationship with nematodes from the family Heterorhabditis, where the bacteria occupy the gut of the infective juvenile (IJ) stage of the nematode. In this study we describe the identif...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.1.77-84.2005

    authors: Bennett HP,Clarke DJ

    更新日期:2005-01-01 00:00:00

  • Regulation of the cadA cadmium resistance determinant of Staphylococcus aureus plasmid pI258.

    abstract::Regulation of the cadA cadmium and zinc resistance determinant of Staphylococcus aureus plasmid pI258 was demonstrated by using gene fusions and direct measurements of transcription. In growth experiments, cells harboring the intact cadA operon were induced with different cations and challenged by an inhibitory concen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.23.7643-7649.1991

    authors: Yoon KP,Misra TK,Silver S

    更新日期:1991-12-01 00:00:00

  • High-frequency variation in Mycoplasma pulmonis colony size.

    abstract::Heterogeneity in colony size of the murine pathogen Mycoplasma pulmonis was examined. Subcloning experiments showed that colony size variation resulted from high-frequency genetic changes. About 3% of the colonies from any given subclone were variants, with as much as a fourfold change in colony diameter. When the var...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.9.5165-5168.1989

    authors: Dybvig K,Simecka JW,Watson HL,Cassell GH

    更新日期:1989-09-01 00:00:00

  • Topological model of the Rhodobacter capsulatus light-harvesting complex I assembly protein LhaA (previously known as ORF1696).

    abstract::A theoretical topology of the Rhodobacter capsulatus membrane protein LhaA was formulated and evaluated by gene fusion experiments. The apparent topological locations of fusion enzymes were compared with the theoretically derived structure, and a model of LhaA is suggested that consists of 12 transmembrane segments, w...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.17.4742-4745.1998

    authors: Young CS,Beatty JT

    更新日期:1998-09-01 00:00:00

  • Fructose 1,6-bisphosphate aldolase activity is essential for synthesis of alginate from glucose by Pseudomonas aeruginosa.

    abstract::We have isolated a mutant of Pseudomonas aeruginosa deficient in fructose 1,6-bisphosphate aldolase activity. This mutant, similar to the mutants deficient in any of the Entner-Doudoroff pathway enzymes, does not allow appreciable alginate formation from glucose and gluconate, but allows alginate synthesis from mannit...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.161.1.458-460.1985

    authors: Banerjee PC,Vanags RI,Chakrabarty AM,Maitra PK

    更新日期:1985-01-01 00:00:00

  • Site-specific serine incorporation by Lif and Epr into positions 3 and 5 of the Staphylococcal peptidoglycan interpeptide bridge.

    abstract::The FemAB-like factors Lif and Epr confer resistance to glycylglycine endopeptidases lysostaphin and Ale-1, respectively, by incorporating serine residues into the staphylococcal peptidoglycan interpeptide bridges specifically at positions 3 and 5. This required the presence of FemA and/or FemB, in contrast to earlier...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.9.2635-2638.2000

    authors: Ehlert K,Tschierske M,Mori C,Schröder W,Berger-Bächi B

    更新日期:2000-05-01 00:00:00

  • Phosphorylation of the group A Streptococcal CovR response regulator causes dimerization and promoter-specific recruitment by RNA polymerase.

    abstract::The group A streptococcus (GAS), Streptococcus pyogenes, is an important human pathogen that causes infections ranging in severity from self-limiting pharyngitis to severe invasive diseases that are associated with significant morbidity and mortality. The pathogenic effects of GAS are mediated by the expression of vir...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00198-06

    authors: Gusa AA,Gao J,Stringer V,Churchward G,Scott JR

    更新日期:2006-07-01 00:00:00

  • Identification of two quorum-sensing systems in Sinorhizobium meliloti.

    abstract::Sinorhizobium meliloti is a free-living soil bacterium which is capable of establishing a symbiotic relationship with the alfalfa plant (Medicago sativa). This symbiosis involves a network of bacterium-host signaling, as well as the potential for bacterium-bacterium communication, such as quorum sensing. In this study...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.13.3466-3475.2002

    authors: Marketon MM,González JE

    更新日期:2002-07-01 00:00:00

  • Comparison of the two isofunctional enol-lactone hydrolases from Acinetobacter calcoaceticus.

    abstract::The rates of thermal denaturation and the molecular weights of the two isofunctional enol-lactone hydrolases (ELH I and ELH II) of Acinetobacter calcoaceticus were determined. The molecular weights of ELH I and ELH II were found by gel filtration to be approximately 24,000 and 21,000, respectively. In crude extract at...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.2.369-374.1971

    authors: Katagiri M,Wheelis ML

    更新日期:1971-05-01 00:00:00

  • Physiology of Sporeforming Bacteria Associated with Insects II. Lipids of Vegetative Cells.

    abstract::Lipid composition was studied in two strains each of mid-log phase cells of Bacillus thuringiensis, B. larvae, B. popilliae, B. alvei, and B. lentimorbus. Total lipids varied from 2.5 to 3.5% of the cell dry weight of B. thuringiensis to 4.3 to 5.0% of B. popilliae. Phospholipids in the organisms examined ranged from ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.104.3.1246-1253.1970

    authors: Bulla LA,Bennett GA,Shotwell OL

    更新日期:1970-12-01 00:00:00

  • Effects of mutations to streptomycin resistance on the rate of translation of mutant genetic information.

    abstract::Gartner, T. K. (University of California, Santa Barbara), and E. Orias. Effects of mutations to streptomycin resistance on the rate of translation of mutant genetic information. J. Bacteriol. 91:1021-1028. 1966.-The effects of mutations to streptomycin resistance of independent origin upon the translation of suppressi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.3.1021-1028.1966

    authors: Gartner TK,Orias E

    更新日期:1966-03-01 00:00:00

  • Genetic analysis of nonpathogenic Agrobacterium tumefaciens mutants arising in crown gall tumors.

    abstract::Little is known about the effect of the host on the genetic stability of bacterial plant pathogens. Crown gall, a plant disease caused by Agrobacterium tumefaciens, may represent a useful model to study this effect. Indeed, our previous observations on the natural occurrence and origin of nonpathogenic agrobacteria su...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.13.3752-3757.1995

    authors: Bélanger C,Canfield ML,Moore LW,Dion P

    更新日期:1995-07-01 00:00:00

  • Regulation of galactose operon expression: glucose effects and role of cyclic adenosine 3',5'-monophosphate.

    abstract::We studied the following two aspects of the glucose effect on galactose operon expression in Escherichia coli K-12: catabolite repression and inducer exclusion. Using both inducible and constitutive strains and measuring the rate of promoter-proximal enzyme synthesis, we found that the galactose operon did not seem to...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.146.1.149-154.1981

    authors: Joseph E,Danchin A,Ullmann A

    更新日期:1981-04-01 00:00:00

  • Molecular cloning and regulation of expression of the genes for initiation factor 3 and two aminoacyl-tRNA synthetases.

    abstract::A 22-kilobase fragment of the Escherichia coli chromosome which contains the genes for translation initiation factor 3, phenylalanyl-tRNA synthetase, and threonyl-tRNA synthetase was cloned into plasmid pACYC184. The hybrid plasmid (designated pID1) complements a temperature-sensitive pheS lesion in E. coli NP37. pID1...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:

    authors: Elseviers D,Gallagher P,Hoffman A,Weinberg B,Schwartz I

    更新日期:1982-10-01 00:00:00

  • Identification in Listeria monocytogenes of MecA, a homologue of the Bacillus subtilis competence regulatory protein.

    abstract::We identified in Listeria monocytogenes a gene encoding a protein homologous to MecA, a regulatory protein acting with ClpC and ComK in the competence pathway of Bacillus subtilis. In L. monocytogenes, MecA is involved, along with ClpC and ClpP, in the downregulation of a 64-kDa secreted protein. In B. subtilis, the M...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.20.5931-5934.2000

    authors: Borezee E,Msadek T,Durant L,Berche P

    更新日期:2000-10-01 00:00:00

  • Clostridium perfringens spore germination: characterization of germinants and their receptors.

    abstract::Clostridium perfringens food poisoning is caused by type A isolates carrying a chromosomal enterotoxin (cpe) gene (C-cpe), while C. perfringens-associated non-food-borne gastrointestinal (GI) diseases are caused by isolates carrying a plasmid-borne cpe gene (P-cpe). C. perfringens spores are thought to be the importan...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01748-07

    authors: Paredes-Sabja D,Torres JA,Setlow P,Sarker MR

    更新日期:2008-02-01 00:00:00

  • Identification and characterization of genes encoding a putative ABC-type transporter essential for utilization of gamma-hexachlorocyclohexane in Sphingobium japonicum UT26.

    abstract::Sphingobium japonicum UT26 utilizes gamma-hexachlorocyclohexane (gamma-HCH) as its sole source of carbon and energy. In our previous studies, we cloned and characterized genes encoding enzymes for the conversion of gamma-HCH to beta-ketoadipate in UT26. In this study, we analyzed a mutant obtained by transposon mutage...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01883-06

    authors: Endo R,Ohtsubo Y,Tsuda M,Nagata Y

    更新日期:2007-05-01 00:00:00

  • Tryptophanase-tryptophan synthetase systems in Escherichia coli. III. Requirements for enzyne synthesis.

    abstract::Freundlich, Martin (University of Minnesota, Minneapolis) and Herman C. Lichstein. Tryptophanase-tryptophan synthetase systems in Escherichia coli. III. Requirements for enzyme synthesis. J. Bacteriol. 84:996-1006. 1962.-The requirements for the formation of tryptophanase and tryptophan synthetase in Escherichia coli ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.84.5.996-1006.1962

    authors: FREUNDLICH M,LICHSTEIN HC

    更新日期:1962-11-01 00:00:00

  • Chromosome rearrangement and diversification of Francisella tularensis revealed by the type B (OSU18) genome sequence.

    abstract::The gamma-proteobacterium Francisella tularensis is one of the most infectious human pathogens, and the highly virulent organism F. tularensis subsp. tularensis (type A) and less virulent organism F. tularensis subsp. holarctica (type B) are most commonly associated with significant disease in humans and animals. Here...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00506-06

    authors: Petrosino JF,Xiang Q,Karpathy SE,Jiang H,Yerrapragada S,Liu Y,Gioia J,Hemphill L,Gonzalez A,Raghavan TM,Uzman A,Fox GE,Highlander S,Reichard M,Morton RJ,Clinkenbeard KD,Weinstock GM

    更新日期:2006-10-01 00:00:00

  • Class 1 integrons potentially predating the association with tn402-like transposition genes are present in a sediment microbial community.

    abstract::Integrons are genetic elements that contribute to lateral gene transfer in bacteria as a consequence of possessing a site-specific recombination system. This system facilitates the spread of genes when they are part of mobile cassettes. Most integrons are contained within chromosomes and are confined to specific bacte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01950-05

    authors: Stokes HW,Nesbø CL,Holley M,Bahl MI,Gillings MR,Boucher Y

    更新日期:2006-08-01 00:00:00

  • Draft genome sequence of Lactobacillus gigeriorum CRBIP 24.85T, isolated from a chicken crop.

    abstract::We report the draft genome of the strain Lactobacillus gigeriorum CRBIP 24.85(T), isolated from a chicken crop. The total length of the 60 scaffolds is about 1.9 Mb, with a GC content of 38% and 2,062 protein-coding sequences (CDS). ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01427-12

    authors: Cousin S,Ma L,Creno S,Clermont D,Loux V,Bizet C,Bouchier C

    更新日期:2012-11-01 00:00:00

  • Genetic co-regulation of galactose and melibiose utilization in Saccharomyces.

    abstract::The gal3 mutation of Saccharomyces, which is associated with an impairment in the utilization of galactose, has been shown to be pleiotropic, causing similar impairments in the utilization of melibiose and maltose. Milibiose utilization and alpha-galactosidase production are directly controlled by the galactose regula...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.1.33-41.1976

    authors: Kew OM,Douglas HC

    更新日期:1976-01-01 00:00:00

  • Mutations in a new Streptomyces coelicolor locus which globally block antibiotic biosynthesis but not sporulation.

    abstract::Streptomyces coelicolor produces four known antibiotics. To define genetic elements that regulate antibiotic synthesis, we screened for mutations that visibly blocked synthesis of the two pigmented antibiotics and found that the mutant strains which we recovered were of two classes--double mutants and mutants in which...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.2962-2969.1990

    authors: Adamidis T,Riggle P,Champness W

    更新日期:1990-06-01 00:00:00

  • Competence-inducing factor of Bacillus stearothermophilus.

    abstract::An incompetent mutant (4S Com(-)) does not release competence-inducing factor (CF) into the culture medium and is not infected with TP-1C phage deoxyribonucleic acid (DNA) unless CF is added to the transfection assay. A TP-1C phage-resistant mutant (4S Ton-r) releases relatively large amounts of CF into the culture me...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.106.3.955-959.1971

    authors: Streips UN,Welker NE

    更新日期:1971-06-01 00:00:00

  • Regulation of cytotoxicity by quorum-sensing signaling in Vibrio vulnificus is mediated by SmcR, a repressor of hlyU.

    abstract::Cytotoxicity is an important virulence determinant in the pathogenesis of Vibrio vulnificus, and two cytotoxins, RTX (encoded by rtxA1) and cytolysin/hemolysin (encoded by vvhA), have been identified in this organism. We showed that the quorum-sensing regulator LuxO controlled the cytotoxicity of this organism: a Δlux...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01259-10

    authors: Shao CP,Lo HR,Lin JH,Hor LI

    更新日期:2011-05-01 00:00:00

  • Membrane interaction of the glycosyltransferase MurG: a special role for cardiolipin.

    abstract::MurG is a peripheral membrane protein that is one of the key enzymes in peptidoglycan biosynthesis. The crystal structure of Escherichia coli MurG (S. Ha, D. Walker, Y. Shi, and S. Walker, Protein Sci. 9:1045-1052, 2000) contains a hydrophobic patch surrounded by basic residues that may represent a membrane associatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.13.3773-3779.2003

    authors: van den Brink-van der Laan E,Boots JW,Spelbrink RE,Kool GM,Breukink E,Killian JA,de Kruijff B

    更新日期:2003-07-01 00:00:00

  • Amino acid residues in LuxR critical for its mechanism of transcriptional activation during quorum sensing in Vibrio fischeri.

    abstract::PCR-based site-directed mutagenesis has been used to generate 38 alanine-substitution mutations in the C-terminal 41 amino acid residues of LuxR. This region plays a critical role in the mechanism of LuxR-dependent transcriptional activation of the Vibrio fischeri lux operon during quorum sensing. The ability of the v...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.1.387-392.2001

    authors: Trott AE,Stevens AM

    更新日期:2001-01-01 00:00:00

  • ToxR proteins with substitutions in residues conserved with OmpR fail to activate transcription from the cholera toxin promoter.

    abstract::The ToxR protein of Vibrio cholerae is an integral membrane protein that coordinately regulates the expression of virulence genes required for successful infection. ToxR has been shown to bind directly to and activate transcription of the cholera toxin (ctx) promoter. Within the amino-terminal cytoplasmic region of To...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.21.6807-6814.1992

    authors: Ottemann KM,DiRita VJ,Mekalanos JJ

    更新日期:1992-11-01 00:00:00