General method, using Mu-Mud1 dilysogens, to determine the direction of transcription of and generate deletions in the glnA region of Escherichia coli.

abstract：:In Escherichia coli, the CpxRA two-component signal transduction system senses and responds to aggregated and misfolded proteins in the bacterial envelope. We show that CpxR-P (the phosphorylated form of the cognate response regulator) activates cpxRA expression in conjunction with RpoS, suggesting an involvement of t...

journal_title：Journal of bacteriology

authors： De Wulf P,Kwon O,Lin EC

更新日期：1999-11-01 00:00:00

abstract：:DNA uptake by natural competence is a central process underlying the genetic plasticity, biology, and virulence of the human respiratory opportunistic pathogen Streptococcus pneumoniae A study reported in this issue (J. Slager, R. Aprianto, and J.-W. Veening, J. Bacteriol. 201:e00780-18, https://doi.org/10.1128/JB.007...

journal_title：Journal of bacteriology

authors： Winkler ME,Morrison DA

更新日期：2019-06-10 00:00:00

abstract：:The kinetics of beta-lactamase induction in Pseudomonas maltophilia IID1275/873 were investigated. Upon induction with beta-lactam antibiotics, a correlation was seen between the increase in specific beta-lactamase activity and the generation time, as well as the concentration of inducer in the medium. The specific be...

journal_title：Journal of bacteriology

authors： Rosta S,Mett H

更新日期：1989-01-01 00:00:00

abstract：:An alpha-L-arabinofuranosidase (EC 3.2.1.55) from the culture fluid of Rhodotorula flava IFO 0407 grown on beet arabinan as a carbon source has been highly purified. The purified enzyme has a pH optimum of 2.0. The enzyme is unusually acid stable, retaining 82% of its activity after being maintained for 24 h at pH 1.5...

journal_title：Journal of bacteriology

authors： Uesaka E,Sato M,Raiju M,Kaji A

更新日期：1978-03-01 00:00:00

abstract：:Survivors of nitrosoguanidine-treated cultures of a colicinogenic strain of Salmonella typhimurium were tested for spontaneous production of colicin E1. Of about 1,000 colonies tested, 13 produced no (or very narrow) colicin zones. Four of these isolates proved to be more sensitive to ultraviolet (UV) light, X rays, a...

journal_title：Journal of bacteriology

authors： MacPhee DG

更新日期：1970-10-01 00:00:00

abstract：:Many pathogenic bacteria translocate virulence factors into their eukaryotic hosts by means of type IV secretion systems (T4SS) spanning the inner and outer membranes. Genes encoding components of these systems have been identified within the order Rickettsiales based upon their sequence similarities to other prototyp...

journal_title：Journal of bacteriology

authors： Crosby FL,Munderloh UG,Nelson CM,Herron MJ,Lundgren AM,Xiao YP,Allred DR,Barbet AF

更新日期：2020-11-04 00:00:00

abstract：:Electrical events after a light-dark stimulus were studied in the multicellular organism Phormidium uncinatum. Normally, such a stimulus causes the gliding trichome to reverse direction. By directing a large light spot on the end of a batch of trichomes and then switching it off, we achieved synchronization of the tri...

journal_title：Journal of bacteriology

authors： Murvanidze GV,Glagolev AN

更新日期：1982-04-01 00:00:00

abstract：:A 12.6-kb DNA fragment from Streptomyces glaucescens GLA.0 containing the 12 genes for tetracenomycin (TCM) C biosynthesis and resistance enabled Streptomyces lividans to produce TCM C. Transcriptional analysis of the tcmPG intergenic region in this cluster established the presence of two divergent promoters. The tcmI...

journal_title：Journal of bacteriology

authors： Decker H,Hutchinson CR

更新日期：1993-06-01 00:00:00

abstract：:Adaptation in bacterial chemotaxis involves reversible methylation of specific glutamate residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins. The specific sites of methylation in Salmonella enterica and Escherichia coli chemoreceptors, identified 2 decades ago, established a consensus seque...

journal_title：Journal of bacteriology

authors： Perez E,Zheng H,Stock AM

更新日期：2006-06-01 00:00:00

abstract：:Sinorhizobium meliloti, the nitrogen-fixing symbiont of alfalfa, has the ability to catabolize myo-, scyllo-, and D-chiro-inositol. Functional inositol catabolism (iol) genes are required for growth on these inositol isomers, and they play a role during plant-bacterium interactions. The inositol catabolism genes compr...

journal_title：Journal of bacteriology

authors： Kohler PR,Choong EL,Rossbach S

更新日期：2011-10-01 00:00:00

abstract：:Transcription of the virG gene of Agrobacterium tumefaciens was previously shown to be expressed from two tandem promoters and to be responsive to three stimuli: plant-released phenolic compounds, phosphate starvation, and acidic media. In this report, I describe a set of deletions and other alterations of the 5' end ...

journal_title：Journal of bacteriology

authors： Winans SC

更新日期：1990-05-01 00:00:00

abstract：:Sinorhizobium meliloti, a gram-negative soil bacterium, forms a nitrogen-fixing symbiotic relationship with members of the legume family. To facilitate our studies of transcription in S. meliloti, we cloned and characterized the gene for the alpha subunit of RNA polymerase (RNAP). S. meliloti rpoA encodes a 336-amino-...

journal_title：Journal of bacteriology

authors： Peck MC,Gaal T,Fisher RF,Gourse RL,Long SR

更新日期：2002-07-01 00:00:00

abstract：:Chloramphenicol-resistant strains of Staphylococcus aureus contain an inducible enzyme which inactivates chloramphenicol by acetylation in the presence of acetyl coenzyme A. The products of acetylation are chromatographically indistinguishable from those obtained with chloramphenicol-resistant Escherichia coli harbori...

journal_title：Journal of bacteriology

authors： Shaw WV,Brodsky RF

更新日期：1968-01-01 00:00:00

abstract：:The gene for an extremely thermostable oligo-1,6-glucosidase (dextrin-6-alpha-D-glucanohydrolase; EC 3.2.1.10) of obligately thermophilic Bacillus thermoglucosidasius KP1006 was cloned within a 4.2-kilobase HindIII-PvuII fragment of DNA by using the plasmid pUC19 as a vector and Escherichia coli C600 as a host. The ge...

journal_title：Journal of bacteriology

authors： Watanabe K,Iha H,Ohashi A,Suzuki Y

更新日期：1989-02-01 00:00:00

abstract：:Diploid cells of Saccharomyces cerevisiae underwent meiosis and sporulation when placed in 1% potassium acetate sporulation medium. In unbuffered sporulation medium the pH rose very rapidly, reaching pH 8.4 after 2 h of sporulation. Under these conditions, the uptake of radioactive adenine and lysine was extremely lim...

journal_title：Journal of bacteriology

authors： McCusker JH,Haber JE

更新日期：1977-10-01 00:00:00

abstract：:Inhibition of deoxyribonucleic acid (DNA) synthesis in Escherichia coli by chemical inhibitors or by shifting cultures of temperature-sensitive elongation (dnaE and dnaB) or initiation (dnaA) mutants to nonpermissive conditions led to greatly increased synthesis of the enzyme ribonucleoside diphosphate reductase, whic...

journal_title：Journal of bacteriology

authors： Filpula D,Fuchs JA

更新日期：1977-04-01 00:00:00

abstract：:We describe techniques for isolating individual pairs of mating Escherichia coli and observing them under the light microscope. Some pairs achieved close cell-to-cell contact, whereas others remained loosely connected by invisible connections which may be F pili. After 30 min of mating, the pairs were separated and al...

journal_title：Journal of bacteriology

authors： Ou JT,Anderson TF

更新日期：1970-06-01 00:00:00

abstract：:A new pathway for aerobic benzoate oxidation has been postulated for Azoarcus evansii and for a Bacillus stearothermophilus-like strain. Benzoate is first transformed into benzoyl coenzyme A (benzoyl-CoA), which subsequently is oxidized to 3-hydroxyadipyl-CoA and then to 3-ketoadipyl-CoA; all intermediates are CoA thi...

journal_title：Journal of bacteriology

authors： Gescher J,Zaar A,Mohamed M,Schägger H,Fuchs G

更新日期：2002-11-01 00:00:00

abstract：:Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a stepwise fashion, initiating with attachment to the small intestinal epithelium. This attachment is followed by expression of the toxin-coregulated pilus, microcolony formation, and cholera toxin (CT) production. We have r...

journal_title：Journal of bacteriology

authors： Jude BA,Martinez RM,Skorupski K,Taylor RK

更新日期：2009-11-01 00:00:00

abstract：:Electron cryotomography was used to analyze the structure of the Lyme disease spirochete, Borrelia burgdorferi. This methodology offers a new means for studying the native architecture of bacteria by eliminating the chemical fixing, dehydration, and staining steps of conventional electron microscopy. Using electron cr...

journal_title：Journal of bacteriology

authors： Charon NW,Goldstein SF,Marko M,Hsieh C,Gebhardt LL,Motaleb MA,Wolgemuth CW,Limberger RJ,Rowe N

更新日期：2009-01-01 00:00:00

abstract：:The pbpB gene product penicillin-binding protein 3 (PBP3) of Escherichia coli is one of the major targets of beta-lactam antibiotics. At the permissive temperature, the temperature-sensitive pbpBr1 mutant, which was obtained after selection for increased resistance to cephalexin, shows a dramatic change in shape which...

journal_title：Journal of bacteriology

authors： Taschner PE,Ypenburg N,Spratt BG,Woldringh CL

更新日期：1988-10-01 00:00:00

abstract：:During nitrogen-limited growth, transcription of glnA, which codes for glutamine synthetase, requires sigma 54-RNA polymerase and the phosphorylated from the nitrogen regulator I (NRI; also called NtrC). In cells in which the lac promoter controlled expression of the gene coding for NRI, increasing the intracellular c...

journal_title：Journal of bacteriology

authors： Shiau SP,Schneider BL,Gu W,Reitzer LJ

更新日期：1992-01-01 00:00:00

abstract：:Streptococcus salivarius, a gram-positive bacterium found in the human oral cavity, expresses flexible peritrichous fimbriae. In this paper, we report purification and partial characterization of S. salivarius fimbriae. Fimbriae were extracted by shearing the cell surface of hyperfimbriated mutant A37 (a spontaneous m...

journal_title：Journal of bacteriology

authors： Lévesque C,Vadeboncoeur C,Chandad F,Frenette M

更新日期：2001-05-01 00:00:00

abstract：:Clinical "superbug" isolates of Pseudomonas aeruginosa were previously observed to be resistant to several antibiotics, including polymyxin B, and/or to have a distinct, reproducible adaptive polymyxin resistance phenotype, identified by observing "skipped" wells (appearance of extra turbid wells) during broth microdi...

journal_title：Journal of bacteriology

authors： Boyle B,Fernandez L,Laroche J,Kukavica-Ibrulj I,Mendes CM,Hancock RW,Levesque RC

更新日期：2012-01-01 00:00:00

abstract：:Renaturable regions in the DNA strands of the N group plasmid pCU1 have been visualized as stem-loop structures by electron microscopy. Four such distinct structures are described, the smallest of which is within the loop of a larger one. The region of pCU1 in which these structures occur has several restriction sites...

journal_title：Journal of bacteriology

authors： Konarska-Kozlowska M,Thatte V,Iyer VN

更新日期：1983-03-01 00:00:00

abstract：:Adenosine 5'-triphosphate (ATP) synthesis driven by an artificially imposed membrane potential in right-side-out membrane vesicles of Escherichia coli was investigated. Membrane vesicles prepared in the presence of adenosine diphosphate were loaded with K+ by incubation with 0.5 M potassium phosphate. Addition of vali...

journal_title：Journal of bacteriology

authors： Tsuchiya T,Rosen BP

更新日期：1976-07-01 00:00:00

abstract：:Cell-free extracts of Bacillus licheniformis and B. cereus were found to contain high specific activities of nicotinamide adenine dinucleotide phosphate (NADP)-dependent-l-glutamate dehydrogenase [EC 1.4.1.4; l-glutamate: NADP oxidoreductase (deaminating)]. Maximum specific activities were found in extracts of cells d...

journal_title：Journal of bacteriology

authors： Phibbs PV Jr,Bernlohr RW

更新日期：1971-05-01 00:00:00

abstract：:Among 120 strains of gliding bacteria which were screened for restriction endonucleases, 27 were found positive. Additionally, three strains carried enzymes able to release the supercoiled state of closed circular DNA. By using a new rapid method, restriction endonuclease activity was released by stirring about 0.5 g ...

journal_title：Journal of bacteriology

authors： Mayer H,Reichenbach H

更新日期：1978-11-01 00:00:00

abstract：:As a result of serial exposures to a mutagenic agent, N-methyl-N'-nitro-N-nitrosoguanidine, the yield of enterotoxin A produced by the last mutant in the series was increased nearly 20-fold over the amount produced by the parent Staphylococcus aureus 100. ...

journal_title：Journal of bacteriology

authors： Friedman ME,Howard MB

更新日期：1971-04-01 00:00:00

abstract：:Monoubiquitination of the 12-transmembrane segment (12-TMS) Saccharomyces cerevisiae maltose transporter promoted the maximal internalization rate of this protein. This modification is similar to that of the 7-TMS alpha-factor receptor but different from that of the 12-TMS uracil and general amino acid permeases. This...

journal_title：Journal of bacteriology

authors： Lucero P,Peñalver E,Vela L,Lagunas R

更新日期：2000-01-01 00:00:00