Regulation of synthesis of pyruvate carboxylase in the photosynthetic bacterium Rhodobacter capsulatus.

abstract：:Clinical isolates of the porcine pathogen Actinobacillus pleuropneumoniae often form adherent colonies on agar plates due to expression of an operon, pgaABCD, encoding a poly-beta-1,6-N-acetyl-D-glucosamine (PGA) extracellular matrix. The adherent colony phenotype, which correlates with the ability to form biofilms on...

journal_title：Journal of bacteriology

authors： Bossé JT,Sinha S,Li MS,O'Dwyer CA,Nash JH,Rycroft AN,Kroll JS,Langford PR

更新日期：2010-05-01 00:00:00

abstract：:The Escherichia coli K-12 mtr gene, which encodes a tryptophan-specific permease, was cloned, and its nucleotide sequence was determined. The precise location of the mtr gene at 69 min on the E. coli chromosome was determined. The mtr gene product was identified as a 414-amino-acid residue protein with a calculated mo...

journal_title：Journal of bacteriology

authors： Sarsero JP,Wookey PJ,Pittard AJ

更新日期：1991-07-01 00:00:00

abstract：:Tn916 [carries tet(M)] is a 16.4-kb conjugative transposon that can establish itself in multiple copies in Enterococcus faecalis. To study the interaction of coresident homologous transposons during conjugation, an E. faecalis mutant defective in homologous recombination was utilized for construction of strains harbor...

journal_title：Journal of bacteriology

authors： Flannagan SE,Clewell DB

更新日期：1991-11-01 00:00:00

abstract：:Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...

journal_title：Journal of bacteriology

authors： Lucier TS,Brubaker RR

更新日期：1992-04-01 00:00:00

abstract：:Pertussis toxin (PT), the major virulence factor of Bordetella pertussis, is composed of five different subunits whose genes are organized as an operon. We report the mapping of the promoter region of the PT operon and show that this promoter is only weakly active in Escherichia coli. Bordetella parapertussis and Bord...

journal_title：Journal of bacteriology

authors： Nicosia A,Rappuoli R

更新日期：1987-06-01 00:00:00

abstract：:The application of mitomycin C induction to 114 genetically diverse Streptococcus agalactiae strains generated 36 phage suspensions. On electron microscopy of the phage suspensions, it was possible to assign the phages to the Siphoviridae family, with three different morphotypes (A, B, and C). Phage genetic diversity ...

journal_title：Journal of bacteriology

authors： Domelier AS,van der Mee-Marquet N,Sizaret PY,Héry-Arnaud G,Lartigue MF,Mereghetti L,Quentin R

更新日期：2009-08-01 00:00:00

abstract：:Movement on surfaces, or swarming motility, is effectively mediated by the lateral flagellar (laf) system in Vibrio parahaemolyticus. Expression of laf is induced by conditions inhibiting rotation of the polar flagellum, which is used for swimming in liquid. However, not all V. parahaemolyticus isolates swarm proficie...

journal_title：Journal of bacteriology

authors： Jaques S,McCarter LL

更新日期：2006-04-01 00:00:00

abstract：:Burkholderia sp. strain GG4, isolated from the ginger rhizosphere, possesses a unique N-acylhomoserine lactone (AHL)-modifying activity that reduces 3-oxo-AHLs to 3-hydroxy-AHLs. To the best of our knowledge, this is the first sequenced genome from a bacterium of the genus Burkholderia that shows both quorum-sensing a...

journal_title：Journal of bacteriology

authors： Hong KW,Koh CL,Sam CK,Yin WF,Chan KG

更新日期：2012-11-01 00:00:00

abstract：:The kinA (spoIIJ) locus contains a single gene which codes for a protein of 69,170 daltons showing strong homology to the transmitter kinases of two component regulatory systems. The purified kinase autophosphorylates in the presence of ATP and mediates the transfer of phosphate to the Spo0A and Spo0F sporulation regu...

journal_title：Journal of bacteriology

authors： Perego M,Cole SP,Burbulys D,Trach K,Hoch JA

更新日期：1989-11-01 00:00:00

abstract：:Sinorhizobium meliloti is a soil bacterium capable of invading and establishing a symbiotic relationship with alfalfa plants. This invasion process requires the synthesis, by S. meliloti, of at least one of the two symbiotically important exopolysaccharides, succinoglycan and EPS II. We have previously shown that the ...

journal_title：Journal of bacteriology

authors： Marketon MM,Glenn SA,Eberhard A,González JE

更新日期：2003-01-01 00:00:00

abstract：:In order to analyze in molecular terms how Sulfolobus genomes diverge, damage-induced mutations and natural polymorphisms (PMs) were identified in laboratory constructs and wild-type isolates, respectively, of Sulfolobus acidocaldarius Among wild-type isolates drawn from one local population, pairwise nucleotide diver...

journal_title：Journal of bacteriology

authors： Mao D,Grogan DW

更新日期：2017-08-08 00:00:00

abstract：:A new strategy was developed for rapid cloning of genes with a transposon mutation library. We constructed a transposon designated TnV that was derived from Tn5 and consists of the gene coding for neomycin phosphotransferase II as well as the replication origin of an Escherichia coli plasmid, pSC101, flanked by Tn5 in...

journal_title：Journal of bacteriology

authors： Furuichi T,Inouye M,Inouye S

更新日期：1985-10-01 00:00:00

abstract：:For maximal turbidity and dissimilatory activity, three strains of pediococci required the addition of Mn(2+) to Rogosa basal medium. Twenty-two strains of enterococci proved indifferent to this supplement. ...

journal_title：Journal of bacteriology

authors： Efthymiou CJ,Joseph SW

更新日期：1972-10-01 00:00:00

abstract：:The T-POP transposon was employed in a general screen for tetracycline (Tet)-induced chromosomal loci that exhibited Tet-activated or Tet-repressed expression of a fliC-lac transcriptional fusion. Insertions that activated flagellar transcription were located in flagellar genes. T-POP insertions that exhibited Tet-dep...

journal_title：Journal of bacteriology

authors： Wozniak CE,Lee C,Hughes KT

更新日期：2009-03-01 00:00:00

abstract：:Streptomyces clavuligerus produces at least five different clavam metabolites, including clavulanic acid and the methionine antimetabolite, alanylclavam. In vitro transposon mutagenesis was used to analyze a 13-kb region upstream of the known paralogue gene cluster. The paralogue cluster includes one group of clavulan...

journal_title：Journal of bacteriology

authors： Zelyas NJ,Cai H,Kwong T,Jensen SE

更新日期：2008-12-01 00:00:00

abstract：:Short-term labeling with radioactive thymidine led to inaccurate estimates of deoxyribonucleic acid synthesis at some growth rates in steady-state cultures of Escherichia coli B/r. Estimates were corrected in chemostat cultures by adding adenosine, a known inhibitor of thymidine phosphorylase. ...

journal_title：Journal of bacteriology

authors： Kubitschek HE,Valdez D,Freedman ML

更新日期：1972-06-01 00:00:00

abstract：:We previously described a bacteriophage of the Lyme disease agent Borrelia burgdorferi designated phiBB-1. This phage packages the host complement of the 32-kb circular plasmids (cp32s), a group of homologous molecules found throughout the genus Borrelia. To demonstrate the ability of phiBB-1 to package and transduce ...

journal_title：Journal of bacteriology

authors： Eggers CH,Kimmel BJ,Bono JL,Elias AF,Rosa P,Samuels DS

更新日期：2001-08-01 00:00:00

abstract：:Sinorhizobium meliloti can live as a soil saprophyte and can engage in a nitrogen-fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma (σ) factors interacting wit...

journal_title：Journal of bacteriology

authors： Barnett MJ,Bittner AN,Toman CJ,Oke V,Long SR

更新日期：2012-09-01 00:00:00

abstract：:The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C...

journal_title：Journal of bacteriology

authors： Duncan CL,Sugiyama H,Strong DH

更新日期：1968-05-01 00:00:00

abstract：:Protein export in Escherichia coli is mediated by translocase, a multisubunit membrane protein complex with SecA as the peripheral subunit and the SecY, SecE, and SecG proteins as the integral membrane domain. In the gram-positive bacterium Bacillus subtilis, SecA, SecY, and SecE have been identified through genetic a...

journal_title：Journal of bacteriology

authors： van Wely KH,Swaving J,Broekhuizen CP,Rose M,Quax WJ,Driessen AJ

更新日期：1999-03-01 00:00:00

abstract：:Alcaligenes eutrophus H16 produces two [NiFe] hydrogenases which catalyze the oxidation of hydrogen and enable the organism to utilize H2 as the sole energy source. The genes (hoxK and hoxG) for the heterodimeric, membrane-bound hydrogenase (MBH) are located adjacent to a series of eight accessory genes (hoxZ, hoxM, h...

journal_title：Journal of bacteriology

authors： Bernhard M,Schwartz E,Rietdorf J,Friedrich B

更新日期：1996-08-01 00:00:00

abstract：:The FemAB-like factors Lif and Epr confer resistance to glycylglycine endopeptidases lysostaphin and Ale-1, respectively, by incorporating serine residues into the staphylococcal peptidoglycan interpeptide bridges specifically at positions 3 and 5. This required the presence of FemA and/or FemB, in contrast to earlier...

journal_title：Journal of bacteriology

authors： Ehlert K,Tschierske M,Mori C,Schröder W,Berger-Bächi B

更新日期：2000-05-01 00:00:00

abstract：:The disaccharide trehalose is accumulated as a storage product by spores of Streptomyces griseus. Nongerminating spores used their trehalose reserves slowly when incubated in buffer for several months. In contrast, spores rapidly depleted their trehalose pools during the first hours of germination. Extracts of dormant...

journal_title：Journal of bacteriology

authors： McBride MJ,Ensign JC

更新日期：1987-11-01 00:00:00

abstract：:Poly-beta-hydroxybutyrate (PHB) synthesis in a cyanobacterium, Synechococcus sp. strain MA19, is controlled at the enzyme level and is dependent on the C/N balance in the culture medium. The control involves at least two enzymes. The first enzyme is PHB synthase. Little PHB synthase activity was detected in crude extr...

journal_title：Journal of bacteriology

authors： Miyake M,Kataoka K,Shirai M,Asada Y

更新日期：1997-08-01 00:00:00

abstract：:The hemF gene of Rhodobacter sphaeroides 2.4.1 is predicted to code for an oxygen-dependent coproporphyrinogen III oxidase. We found that a HemF- mutant strain is unable to grow under aerobic conditions. We also determined that hemF expression is controlled by oxygen, which is mediated, at least in part, by the respon...

journal_title：Journal of bacteriology

authors： Zeilstra-Ryalls JH,Schornberg KL

更新日期：2006-01-01 00:00:00

abstract：:The global transcriptional regulator Spx of Bacillus subtilis is controlled at several levels of the gene expression process. It is maintained at low concentrations during unperturbed growth by the ATP-dependent protease ClpXP. Under disulfide stress, Spx concentration increases due in part to a reduction in ClpXP-cat...

journal_title：Journal of bacteriology

authors： Garg SK,Kommineni S,Henslee L,Zhang Y,Zuber P

更新日期：2009-02-01 00:00:00

abstract：:Pathogenic Yersinia species employ a type III secretion system (TTSS) to target antihost factors, Yop proteins, into eukaryotic cells. The secretion machinery is constituted of ca. 20 Ysc proteins, nine of which show significant homology to components of the flagellar TTSS. A key event in flagellar assembly is the swi...

journal_title：Journal of bacteriology

authors： Lavander M,Sundberg L,Edqvist PJ,Lloyd SA,Wolf-Watz H,Forsberg A

更新日期：2002-08-01 00:00:00

abstract：:Two different 30-nucleotide regions of the cryIAc insecticidal protoxin gene from Bacillus thuringiensis were randomly mutagenized. One region was within one of seven amphipathic helices believed to be important for the formation of ion channels. There was no loss of toxicity for three test insects by any of 27 mutant...

journal_title：Journal of bacteriology

authors： Aronson AI,Wu D,Zhang C

更新日期：1995-07-01 00:00:00

abstract：:Bacillus subtilis contains three Fur homologs: Fur, PerR, and Zur. Despite significant sequence similarities, they respond to different stimuli and regulate different sets of genes. DNA target site comparisons indicate that all three paralogs recognize operators with a core 7-1-7 inverted repeat. The corresponding con...

journal_title：Journal of bacteriology

authors： Fuangthong M,Helmann JD

更新日期：2003-11-01 00:00:00

abstract：:The seven proteins encoded by the comG operon of Bacillus subtilis exhibit similarity to gene products required for the assembly of type 4 pili and for the secretion of certain proteins in gram-negative bacteria. Although polar transposon insertions in comG result in the loss of transformability and in the failure of ...

journal_title：Journal of bacteriology

authors： Chung YS,Dubnau D

更新日期：1998-01-01 00:00:00