Identification and characterization of a novel member of the radical AdoMet enzyme superfamily and implications for the biosynthesis of the Hmd hydrogenase active site cofactor.

abstract：:We demonstrate that expression of the UGA1, CAN1, GAP1, PUT1, PUT2, PUT4, and DAL4 genes is sensitive to nitrogen catabolite repression. The expression of all these genes, with the exception of UGA1 and PUT2, also required a functional GLN3 protein. In addition, GLN3 protein was required for expression of the DAL1, DA...

journal_title：Journal of bacteriology

authors： Daugherty JR,Rai R,el Berry HM,Cooper TG

更新日期：1993-01-01 00:00:00

abstract：:During the growth of Clostridium cellulolyticum in chemostat cultures with ammonia as the growth-limiting nutrient, as much as 30% of the original cellobiose consumed by C. cellulolyticum was converted to cellotriose, glycogen, and polysaccharides regardless of the specific growth rates. Whereas the specific consumpti...

journal_title：Journal of bacteriology

authors： Guedon E,Desvaux M,Petitdemange H

更新日期：2000-04-01 00:00:00

abstract：:Strains of Staphylococcus aureus, an important human pathogen, display up to 20% variability in their genome sequence, and most sequence information is available for human clinical isolates that have not been subjected to genetic analysis of virulence attributes. S. aureus strain Newman, which was also isolated from a...

journal_title：Journal of bacteriology

authors： Baba T,Bae T,Schneewind O,Takeuchi F,Hiramatsu K

更新日期：2008-01-01 00:00:00

abstract：:Undecyl acetate esterase has been purified from Pseudomonas cepacia grown on the methyl ketone, 2-tridecanone. The K(m) for undecyl acetate was 2.3 x 10(-2) M. Polyacrylamide gel electrophoresis indicated that two esterase bands were being recovered during purification. These bands were separated by preparative polyac...

journal_title：Journal of bacteriology

authors： Shum AC,Markovetz AJ

更新日期：1974-06-01 00:00:00

abstract：:The first step in the transfer of the Bacteroides conjugative transposon CTnDOT is excision of the integrated element from the chromosome to form a circular transfer intermediate. Excision occurs only after the bacteria are exposed to tetracycline. Previously, four excision genes were identified. One was the integrase...

journal_title：Journal of bacteriology

authors： Moon K,Shoemaker NB,Gardner JF,Salyers AA

更新日期：2005-08-01 00:00:00

abstract：:In the microbial conversion of added hypoxanthine to 5'-inosinic acid, Mn(2+) concentration in the growth medium is known to have a profound effect both on the yield of 5'-inosinic acid and the morphology of cells of Brevibacterium ammoniagenes. To elucidate the mechanism in which Mn(2+) was concerned with cell morpho...

journal_title：Journal of bacteriology

authors： Oka T,Udagawa K,Kinoshita S

更新日期：1968-11-01 00:00:00

abstract：:Wzc(CPS) is a tyrosine autokinase essential for the assembly of a high-molecular-weight (HMW) group 1 capsular polysaccharide (CPS) in Escherichia coli. Homologues of Wzc participate in the formation of CPS and exopolysaccharides in a variety of gram-positive and gram-negative bacteria. Phosphorylation of tyrosine res...

journal_title：Journal of bacteriology

authors： Paiment A,Hocking J,Whitfield C

更新日期：2002-12-01 00:00:00

abstract：:The TOL plasmid originally isolated in Pseudomonas putida (arvilla) mt-2 was transmissible to strains of the fluorescens group of Pseudomonas, i.e., P. putida, P. fluorescens, and P. aeruginosa, except for a strain of P. aeruginosa, strain PAO. The same strain, however, could accept the plasmid when its restriction an...

journal_title：Journal of bacteriology

authors： Nakazawa T

更新日期：1978-02-01 00:00:00

abstract：:The Escherichia coli purine repressor, PurR, binds to a 16-bp operator sequence and coregulates the genes for de novo synthesis of purine and pyrimidine nucleotides, formation of a one-carbon unit for biosynthesis, and deamination of cytosine. We have characterized the purified repressor. Chemical cross-linking indica...

journal_title：Journal of bacteriology

authors： Choi KY,Zalkin H

更新日期：1992-10-01 00:00:00

abstract：:Small subunit rRNA sequences have been determined for 10 of the most clinically important pathogenic species of the yeast genus Candida (including Torulopsis [Candida] glabrata and Yarrowia [Candida] lipolytica) and for Hansenula polymorpha. Phylogenetic analyses of these sequences and those of Saccharomyces cerevisia...

journal_title：Journal of bacteriology

authors： Barns SM,Lane DJ,Sogin ML,Bibeau C,Weisburg WG

更新日期：1991-04-01 00:00:00

abstract：:A method for the transformation of Bacteroides fragilis with plasmid DNA was developed by using the clindamycin resistance plasmid pBFTM10 as the source of transforming DNA. The method was technically simple to perform and resulted in an average of 4.2 X 10(3) transformants per microgram of pBFTM10 added. A method for...

journal_title：Journal of bacteriology

authors： Smith CJ

更新日期：1985-10-01 00:00:00

abstract：:An exonuclease III-deficient strain of Escherichia coli K-12, BW2001 (xthA11), was unable to perform rapid repair of X-ray-induced deoxyribonucleic acid single-strand breaks and appeared to have a defect in the priming of the 3'-termini necessary for initiation of repair synthesis at the breaks. This defect cannot be ...

journal_title：Journal of bacteriology

authors： Seeberg E,Steinum AL

更新日期：1980-03-01 00:00:00

abstract：:Neurospora crassa mutants deficient in asparagine synthetase were selected by using the procedure of inositol-less death. Complementation tests among the 100 mutants isolated suggested that their alterations were genetically allelic. Recombination analysis with strain S1007t, an asparagine auxotroph, indicated that th...

journal_title：Journal of bacteriology

authors： MacPhee KG,Nelson RE,Schuster SM

更新日期：1983-10-01 00:00:00

abstract：:A comparison of the spectra of spontaneous growth-dependent and adaptive mutations in ebgR shows that both spectra are dominated by insertion sequence (IS)-mediated mutations. The difference between growth-dependent mutations (61% IS mediated) and adaptive mutations (80% IS mediated) is highly significant (P < 0.0001)...

journal_title：Journal of bacteriology

authors： Hall BG

更新日期：1999-02-01 00:00:00

abstract：:The bacterium Rhodobacter capsulatus is capable of an unusual form of genetic exchange, mediated by a transducing bacteriophage-like particle called the gene transfer agent (GTA). GTA production by R. capsulatus is controlled at the level of transcription by a cellular two-component signal transduction system that inc...

journal_title：Journal of bacteriology

authors： Lang AS,Beatty JT

更新日期：2002-02-01 00:00:00

abstract：:Thiocyanate hydrolase is a newly found enzyme from Thiobacillus thioparus THI 115 that converts thiocyanate to carbonyl sulfide and ammonia (Y. Katayama, Y. Narahara, Y. Inoue, F. Amano, T. Kanagawa, and H. Kuraishi, J. Biol. Chem. 267:9170-9175, 1992). We have cloned and sequenced the scn genes that encode the three ...

journal_title：Journal of bacteriology

authors： Katayama Y,Matsushita Y,Kaneko M,Kondo M,Mizuno T,Nyunoya H

更新日期：1998-05-01 00:00:00

abstract：:NAD- and glutathione-dependent formaldehyde dehydrogenase (GD-FALDH) of Paracoccus denitrificans has been purified as a tetramer with a relative molecular mass of 150 kDa. The gene encoding GD-FALDH (flhA) has been isolated, sequenced, and mutated by insertion of a kanamycin resistance gene. The mutant strain is not a...

journal_title：Journal of bacteriology

authors： Ras J,Van Ophem PW,Reijnders WN,Van Spanning RJ,Duine JA,Stouthamer AH,Harms N

更新日期：1995-01-01 00:00:00

abstract：:Proteus swarming is the rapid cyclical population migration across surfaces by elongated cells that hyperexpress flagellar and virulence genes. The mini-Tn5 transposon mutant mns2 was isolated as a tight nonswarming mutant that did not elongate or upregulate flagellar and hemolysin genes. Individual cell motility was ...

journal_title：Journal of bacteriology

authors： Hay NA,Tipper DJ,Gygi D,Hughes C

更新日期：1997-08-01 00:00:00

abstract：:A DNA region carrying lysS, the gene encoding the lysyl-tRNA synthetase, was cloned from the extreme thermophile prokaryote Thermus thermophilus VK-1 and sequenced. The analysis indicated an open reading frame encoding a protein of 492 amino acids. This putative protein has significant homologies to previously sequenc...

journal_title：Journal of bacteriology

authors： Chen J,Brevet A,Lapadat-Tapolsky M,Blanquet S,Plateau P

更新日期：1994-05-01 00:00:00

abstract：:The nucleotide sequences of the Rhodobacter sphaeroides hemA and hemT genes, encoding 5-aminolevulinic acid (ALA) synthase isozymes, were determined. ALA synthase catalyzes the condensation of glycine and succinyl coenzyme A, the first and rate-limiting step in tetrapyrrole biosynthesis. The hemA and hemT structural g...

journal_title：Journal of bacteriology

authors： Neidle EL,Kaplan S

更新日期：1993-04-01 00:00:00

abstract：:spo0A and spo0H are needed for the initiation of sporulation and for the development of genetic competence in Bacillus subtilis. Transcription of spo0A initiates from two promoters, Pv and Ps. Pv is active during vegetative growth and is recognized by RNA polymerase containing sigma A. Expression from Ps increases dur...

journal_title：Journal of bacteriology

authors： Siranosian KJ,Grossman AD

更新日期：1994-06-01 00:00:00

abstract：:In earlier studies from this laboratory, Xanthomonas campestris pv. glycines was found to exhibit a nutrition stress-related postexponential rapid cell death (RCD). The RCD was exhibited in protein-rich media but not in starch or other minimal media. This RCD in X. campestris pv. glycines was found to display features...

journal_title：Journal of bacteriology

authors： Raju KK,Gautam S,Sharma A

更新日期：2006-08-01 00:00:00

abstract：:Escherichia coli UM146 was isolated from the ileum of a Crohn's disease patient. It adheres to and invades enterocytes and can replicate inside macrophages. Its complete genome sequence reveals that it is most closely related to the human urinary tract pathogen E. coli CFT073, but it has a host of genes that are novel...

journal_title：Journal of bacteriology

authors： Krause DO,Little AC,Dowd SE,Bernstein CN

更新日期：2011-01-01 00:00:00

abstract：:Accumulation of l-alpha-glycerophosphate, in cells of Bacillus subtilis mutants lacking the nicotinamide adenine dinucleotide-independent glycerophosphate dehydrogenase activity, suppresses both growth and sporulation. After growth has stopped, the cells slowly develop one and later more asymmetric septa that are thic...

journal_title：Journal of bacteriology

authors： O YK,Freese EB,Freese E

更新日期：1973-02-01 00:00:00

abstract：:The relationship between specific growth rate of Escherichia coli and the concentration of limiting nutrient (glucose or phosphate or tryptophan) has been determined for populations in a steady state. At high concentrations the specific growth rate is independent of the concentration of nutrient, but at low concentrat...

journal_title：Journal of bacteriology

authors： Shehata TE,Marr AG

更新日期：1971-07-01 00:00:00

abstract：:Proteus mirabilis swarming behavior is characterized by the development of concentric rings of growth that are formed as cyclic events of swarmer cell differentiation, swarming migration, and cellular differentiation are repeated during colony translocation across a surface. This cycle produces the bull's-eye colony o...

journal_title：Journal of bacteriology

authors： Belas R,Schneider R,Melch M

更新日期：1998-12-01 00:00:00

abstract：:Numerous field isolates of Moraxella bovis have previously been classified by serological techniques into seven serogroups, each defined by homologous cross-reaction with antisera prepared against purified pili of a single prototype strain. The gene encoding pilin from each of the prototype strains has been characteri...

journal_title：Journal of bacteriology

authors： Atwell JL,Tennent JM,Lepper AW,Elleman TC

更新日期：1994-08-01 00:00:00

abstract：:The genomic organization of the acidophilic chemolithoautotrophic bacterium Thiobacillus ferrooxidans ATCC 21834 has been studied by pulsed-field gel electrophoresis (PFGE). Analysis of its intact DNA, as well as the restriction patterns obtained with several endonucleases, allowed the characterization of one circular...

journal_title：Journal of bacteriology

authors： Irazabal N,Marín I,Amils R

更新日期：1997-03-01 00:00:00

abstract：:The cytochrome c(3) of Desulfovibrio desulfuricans and that of D. vulgaris were purified to homogeneity as judged by disc gel electrophoresis and by ultracentrifugation. Both cytochromes had an oxidation-reduction potential of -205 +/- 5 mv at pH 7.0 and showed characteristic absorption bands at 525 and 553 nm in the ...

journal_title：Journal of bacteriology

authors： Drucker H,Campbell LL

更新日期：1969-10-01 00:00:00

abstract：:Correct targeting of membrane proteins is essential for membrane integrity, cell physiology, and viability. Cotranslational targeting depends on the universally conserved signal recognition particle (SRP), which is a ribonucleoprotein complex comprised of the protein component Ffh and the 4.5S RNA in Escherichia coli ...

journal_title：Journal of bacteriology

authors： Sauerbrei B,Arends J,Schünemann D,Narberhaus F

更新日期：2020-06-25 00:00:00