Role and regulation of Bacillus subtilis glutamate dehydrogenase genes.

Abstract:

:The complete Bacillus subtilis genome contains two genes with the potential to encode glutamate dehydrogenase (GlutDH) enzymes. Mutations in these genes were constructed and characterized. The rocG gene proved to encode a major GlutDH whose synthesis was induced in media containing arginine or ornithine or, to a lesser degree, proline and was repressed by glucose. A rocG null mutant was impaired in utilization of arginine, ornithine, and proline as nitrogen or carbon sources. The gudB gene was expressed under all growth conditions tested but codes for a GlutDH that seemed to be intrinsically inactive. Spontaneous mutations in gudB that removed a 9-bp direct repeat within the wild-type gudB sequence activated the GudB protein and allowed more-efficient utilization of amino acids of the glutamate family.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Belitsky BR,Sonenshein AL

subject

Has Abstract

pub_date

1998-12-01 00:00:00

pages

6298-305

issue

23

eissn

0021-9193

issn

1098-5530

journal_volume

180

pub_type

杂志文章
  • Molecular, genetic, and biochemical characterization of the serC gene of Methanosarcina barkeri Fusaro.

    abstract::The Methanosarcina barkeri serC gene, encoding phosphoserine aminotransferase, was cloned by complementation of an Escherichia coli serC mutant, and its nucleotide sequence was determined. The M. barkeri SerC protein shares significant homology with other known SerC proteins. E. coli serC hosts carrying the cloned gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.19.5797-5802.1996

    authors: Metcalf WW,Zhang JK,Shi X,Wolfe RS

    更新日期:1996-10-01 00:00:00

  • Three new NifA-regulated genes in the Bradyrhizobium japonicum symbiotic gene region discovered by competitive DNA-RNA hybridization.

    abstract::The so-called symbiotic region of the Bradyrhizobium japonicum chromosome (C. Kündig, H. Hennecke, and M. Göttfert, J. Bacteriol. 175:613-622, 1993) was screened for the presence of genes controlled by the nitrogen fixation regulatory protein NifA. Southern blots of restriction enzyme-digested cosmids that represent a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.182.6.1472-1480.2000

    authors: Nienaber A,Huber A,Göttfert M,Hennecke H,Fischer HM

    更新日期:2000-03-01 00:00:00

  • Determination of Wolbachia genome size by pulsed-field gel electrophoresis.

    abstract::Genome sizes of six different Wolbachia strains from insect and nematode hosts have been determined by pulsed-field gel electrophoresis of purified DNA both before and after digestion with rare-cutting restriction endonucleases. Enzymes SmaI, ApaI, AscI, and FseI cleaved the studied Wolbachia strains at a small number...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.7.2219-2225.2001

    authors: Sun LV,Foster JM,Tzertzinis G,Ono M,Bandi C,Slatko BE,O'Neill SL

    更新日期:2001-04-01 00:00:00

  • Induction of the autolytic system of Escherichia coli by specific insertion of bacteriophage MS2 lysis protein into the bacterial cell envelope.

    abstract::Bacterial lysis induced by the expression of the cloned lysis gene of the RNA bacteriophage MS2 in Escherichia coli was shown to be under the same regulatory control mechanisms as penicillin-induced lysis. It was controlled by the stringent response and showed the phenomenon of tolerance when E. coli was grown at pH 5...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.11.5027-5033.1988

    authors: Walderich B,Ursinus-Wössner A,van Duin J,Höltje JV

    更新日期:1988-11-01 00:00:00

  • Regulation of synthesis of pyruvate carboxylase in the photosynthetic bacterium Rhodobacter capsulatus.

    abstract::The synthesis of pyruvate carboxylase (PC) was studied by using quantitative immunoblot analysis with an antibody raised against PC purified from Rhodobacter capsulatus and was found to vary 20-fold depending on the growth conditions. The PC content was high in cells grown on pyruvate or on carbon substrates metaboliz...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.5.1460-1468.1997

    authors: Yakunin AF,Hallenbeck PC

    更新日期:1997-03-01 00:00:00

  • Light-induced lysis and carotenogenesis in Myxococcus xanthus.

    abstract::Burchard, Robert P. (University of Minnesota, Minneapolis), and Martin Dworkin. Light-induced lysis and carotenogenesis in Myxococcus xanthus. J. Bacteriol. 91:535-545. 1966.-Myxococcus xanthus, grown vegetatively in the light, developed an orange carotenoid after the cells entered stationary phase of growth; pigment ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.2.535-545.1966

    authors: Burchard RP,Dworkin M

    更新日期:1966-02-01 00:00:00

  • Stringent response regulation of biofilm formation in Vibrio cholerae.

    abstract::Biofilm formation is a key factor in Vibrio cholerae environmental survival and host colonization. Production of biofilm enables V. cholerae to survive and persist in aquatic environments and aids in the passage through the gastric acid barrier to allow access to the small intestine. The genes involved in biofilm form...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00014-12

    authors: He H,Cooper JN,Mishra A,Raskin DM

    更新日期:2012-06-01 00:00:00

  • Oxaluric acid: a non-metabolizable inducer of the allantoin degradative enzymes in Saccharomyces cerevisiae.

    abstract::Saccharomyces cerevisiae degrades allantoin in five steps to ammonia, CO(2), and glyoxylate. Previously we demonstrated that allophanic acid, the last intermediate of the pathway, was required for induction of all five degradative enzymes. The data presented here indicate that oxaluric acid, an allophanate analogue, i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.117.3.1240-1247.1974

    authors: Sumrada R,Cooper TG

    更新日期:1974-03-01 00:00:00

  • Permeability of bacterial spores. II. Molecular variables affecting solute permeation.

    abstract::Gerhardt, Philipp (University of Michigan, Ann Arbor) and S. H. Black. Permeability of bacterial spores. II. Molecular variables affecting solute permeation. J. Bacteriol. 82:750-760. 1961.-More than 100 compounds were tested for their uptake by dormant spores of a bacillus. The extent of penetration was found to be d...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.82.5.750-760.1961

    authors: GERHARDT P,BLACK SH

    更新日期:1961-11-01 00:00:00

  • Mutations to spectinomycin resistance which alleviate the restriction of an amber suppressor by streptomycin resistance.

    abstract::Unlike mutations to streptomycin resistance, which restrict the function of an SU II amber suppressor, mutations to spectinomycin resistance have no restrictive effect. Furthermore, mutations to spectinomycin resistance counteract the restrictive action of streptomycin-resistant mutations when combined in a single str...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.2.940-943.1969

    authors: Kuwano M,Endo H,Ohnishi Y

    更新日期:1969-02-01 00:00:00

  • Growth kinetics of extremely halophilic archaea (family halobacteriaceae) as revealed by arrhenius plots.

    abstract::Members of the family Halobacteriaceae in the domain Archaea are obligate extreme halophiles. They occupy a variety of hypersaline environments, and their cellular biochemistry functions in a nearly saturated salty milieu. Despite extensive study, a detailed analysis of their growth kinetics is missing. To remedy this...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.3.923-929.2005

    authors: Robinson JL,Pyzyna B,Atrasz RG,Henderson CA,Morrill KL,Burd AM,Desoucy E,Fogleman RE 3rd,Naylor JB,Steele SM,Elliott DR,Leyva KJ,Shand RF

    更新日期:2005-02-01 00:00:00

  • Listeria monocytogenes relA and hpt mutants are impaired in surface-attached growth and virulence.

    abstract::We describe here the identification and characterization of two Listeria monocytogenes (Tn917-LTV3) relA and hpt transposon insertion mutants that were impaired in growth after attachment to a model surface. Both mutants were unable to accumulate (p)ppGpp in response to amino acid starvation, whereas the wild-type str...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.3.621-628.2002

    authors: Taylor CM,Beresford M,Epton HA,Sigee DC,Shama G,Andrew PW,Roberts IS

    更新日期:2002-02-01 00:00:00

  • Proton pump coupled to cytochrome c oxidase in the cyanobacterium Anacystis nidulans.

    abstract::Intact spheroplasts of the cyanobacterium Anacystis nidulans were found to oxidize various exogenous c-type cytochromes with concomitant proton extrusion. In the coupled state, H+/e stoichiometries close to 1 were measured, regardless of absolute reaction rates. It is concluded that the proton translocation observed i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.1.539-542.1983

    authors: Peschek GA

    更新日期:1983-01-01 00:00:00

  • Mutational analysis of cat-86 gene expression controlled by lactococcal promoters in Lactococcus lactis subsp. lactis and Escherichia coli.

    abstract::Promoters were cloned from the chromosomal DNA of Lactococcus lactis subsp. lactis NP4510 by using promoter-probe vector pGKV210. N-Methyl-N'-nitro-N-nitrosoguanidine-induced mutagenesis of L. lactis-(pBV413), with low-level expression of the cat-86 gene, resulted in enhanced expression. Subcloning and sequencing of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.21.6754-6758.1994

    authors: Bojovic B,Djordjevic G,Banina A,Topisirovic L

    更新日期:1994-11-01 00:00:00

  • Occurrence of homologs of the Escherichia coli lytB gene in gram-negative bacterial species.

    abstract::The Escherichia coli LytB protein regulates the activity of guanosine 3',5'-bispyrophosphate synthetase I (RelA). A Southern blot analysis of chromosomal DNA with the E. coli lytB gene as a probe revealed the presence of lytB homologs in all of the gram-negative bacterial species examined but not in gram-positive spec...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.7.1959-1961.1998

    authors: Potter S,Yang X,Boulanger MJ,Ishiguro EE

    更新日期:1998-04-01 00:00:00

  • AbrB modulates expression and catabolite repression of a Bacillus subtilis ribose transport operon.

    abstract::A Bacillus subtilis ribose transport operon (rbs) was shown to be subject to AbrB-mediated control through direct AbrB-DNA binding interactions in the vicinity of the promoter. Overproduction of AbrB was shown to relieve catabolite repression of rbs during growth in the presence of poorer carbon sources such as arabin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.23.6727-6731.1995

    authors: Strauch MA

    更新日期:1995-12-01 00:00:00

  • A novel pheromone quorum-sensing system controls the development of natural competence in Streptococcus thermophilus and Streptococcus salivarius.

    abstract::In streptococcal species, the key step of competence development is the transcriptional induction of comX, which encodes the alternative sigma factor sigma(X), which positively regulates genes necessary for DNA transformation. In Streptococcus species belonging to the mitis and mutans groups, induction of comX relies ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01251-09

    authors: Fontaine L,Boutry C,de Frahan MH,Delplace B,Fremaux C,Horvath P,Boyaval P,Hols P

    更新日期:2010-03-01 00:00:00

  • Streptococcus faecium ATCC 9790 penicillin-binding proteins and penicillin sensitivity are heavily influenced by growth conditions: proposal for an indirect mechanism of growth inhibition by beta-lactams.

    abstract::The effects of variations in growth conditions on the penicillin response of Streptococcus faecium ATCC 9790 were studied. Changes in the growth temperature and medium composition were found to cause striking changes in the bacterial generation time, cellular penicillin sensitivity (minimum inhibitory concentration), ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.154.2.916-923.1983

    authors: Fontana R,Canepari P,Satta G,Coyette J

    更新日期:1983-05-01 00:00:00

  • Thermostable chemotaxis proteins from the hyperthermophilic bacterium Thermotoga maritima.

    abstract::An expressed sequence tag homologous to cheA was previously isolated by random sequencing of Thermotoga maritima cDNA clones (C. W. Kim, P. Markiewicz, J. J. Lee, C. F. Schierle, and J. H. Miller, J. Mol. Biol. 231: 960-981, 1993). Oligonucleotides complementary to this sequence tag were synthesized and used to identi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.2.484-489.1996

    authors: Swanson RV,Sanna MG,Simon MI

    更新日期:1996-01-01 00:00:00

  • Requirement of NifX and other nif proteins for in vitro biosynthesis of the iron-molybdenum cofactor of nitrogenase.

    abstract::The iron-molybdenum cofactor (FeMo-co) of nitrogenase contains molybdenum, iron, sulfur, and homocitrate in a ratio of 1:7:9:1. In vitro synthesis of FeMo-co has been established, and the reaction requires an ATP-regenerating system, dithionite, molybdate, homocitrate, and at least NifB-co (the metabolic product of Ni...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.9.2797-2801.1999

    authors: Shah VK,Rangaraj P,Chatterjee R,Allen RM,Roll JT,Roberts GP,Ludden PW

    更新日期:1999-05-01 00:00:00

  • Presence of multiple sites containing polar material in spherical Escherichia coli cells that lack MreB.

    abstract::In rod-shaped bacteria, certain proteins are specifically localized to the cell poles. The nature of the positional information that leads to the proper localization of these proteins is unclear. In a screen for factors required for the localization of the Shigella sp. actin assembly protein IcsA to the bacterial pole...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.17.6187-6196.2005

    authors: Nilsen T,Yan AW,Gale G,Goldberg MB

    更新日期:2005-09-01 00:00:00

  • Characterization of the functional sites in the oriT region involved in DNA transfer promoted by sex factor plasmid R100.

    abstract::We have previously identified three sites, named sbi, ihfA, and sbyA, specifically recognized or bound by the TraI, IHF, and TraY proteins, respectively; these sites are involved in nicking at the origin of transfer, oriT, of plasmid R100. In the region next to these sites, there exists the sbm region, which consists ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.15.4350-4355.1995

    authors: Abo T,Ohtsubo E

    更新日期:1995-08-01 00:00:00

  • Protein splicing of the Deinococcus radiodurans strain R1 Snf2 intein.

    abstract::Adjacent intein fragments fused to a Snf2/Rad54 helicase-related protein and Snf2/Rad54 helicase were reported for Deinococcus radiodurans R1, leading to the speculation that a frameshift was required for splicing or that trans splicing occurred. However, a type strain (ATCC 13939, RF18410) yielded a single protein th...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.22.6387-6388.2002

    authors: Southworth MW,Perler FB

    更新日期:2002-11-01 00:00:00

  • Specificity and mechanism of tetracycline resistance in a multiple drug resistant strain of Escherichia coli.

    abstract::Izaki, Kazuo (University of Tokyo, Tokyo, Japan), Kan Kiuchi, and Kei Arima. Specificity and mechanism of tetracycline resistance in a multiple drug resistant strain of Escherichia coli. J. Bacteriol. 91:628-633. 1966.-A decrease in the uptake of tetracycline occurred concurrently with a rise in the level of resistanc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.91.2.628-633.1966

    authors: Izaki K,Kiuchi K,Arima K

    更新日期:1966-02-01 00:00:00

  • Dual control of quorum sensing by two TraM-type antiactivators in Agrobacterium tumefaciens octopine strain A6.

    abstract::Agrobacterium tumefaciens wild-type strains have a unique quorum-sensing (QS)-dependent Ti plasmid conjugative transfer phenotype in which QS signaling is activated by corresponding conjugative opine inducers. Strain K588, with a nopaline-type chromosomal background harboring an octopine-type Ti plasmid, however, is a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.7.2435-2445.2006

    authors: Wang C,Zhang HB,Chen G,Chen L,Zhang LH

    更新日期:2006-04-01 00:00:00

  • Multiple determinants of functional mRNA stability: sequence alterations at either end of the lacZ gene affect the rate of mRNA inactivation.

    abstract::The Escherichia coli lacZ gene was used as a model system to identify specific sequence elements affecting mRNA stability. Various insertions and substitutions at the ribosome-binding site increased or decreased the rate of mRNA inactivation by up to fourfold. Deletion of a dyad symmetry, which may give rise to a very...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.7.2167-2172.1991

    authors: Petersen C

    更新日期:1991-04-01 00:00:00

  • VirB3 to VirB6 and VirB8 to VirB11, but not VirB7, are essential for mediating persistence of Brucella in the reticuloendothelial system.

    abstract::The Brucella abortus virB locus contains 12 open reading frames, termed virB1 through virB12, which encode a type IV secretion system. Polar mutations in the virB locus markedly reduce the ability of B. abortus to survive in cultured macrophages or to persist in organs of mice. While a nonpolar deletion of the virB2 g...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00406-08

    authors: den Hartigh AB,Rolán HG,de Jong MF,Tsolis RM

    更新日期:2008-07-01 00:00:00

  • Amino acid antagonist death in Escherichia coli.

    abstract::Six analogues of amino acids killed corresponding auxotrophs of Escherichia coli. With all but one analogue, protein synthesis was required for lethality. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.99.1.336-338.1969

    authors: Rabinovitz M,Finkleman A,Reagan RL,Breitman TR

    更新日期:1969-07-01 00:00:00

  • Cloning and characterization of the hemolysin determinants from Vibrio cholerae RV79(Hly+), RV79(Hly-), and 569B.

    abstract::The Hly region from the chromosome of Vibrio cholerae El Tor strain RV79(Hly-) and the nonhemolytic classical strain 569B were cloned into plasmid vector pBR322. Escherichia coli K-12 transformants possessing these recombinant plasmids were nonhemolytic and were detected with a 32P-labeled hly-specific DNA probe. Rest...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.1.35-41.1985

    authors: Goldberg SL,Murphy JR

    更新日期:1985-04-01 00:00:00

  • An internal region of rpoB is required for autogenous translational regulation of the beta subunit of Escherichia coli RNA polymerase.

    abstract::In order to delineate the region involved in feedback regulation of the RNA polymerase beta subunit (encoded by rpoB), a collection of rpoB-lacZ translational fusions with different endpoints both upstream and downstream of the rpoB start site was assembled on lambda phage vectors. The extent of translational repressi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.22.7174-7179.1992

    authors: Passador L,Linn T

    更新日期:1992-11-01 00:00:00