Abstract:
:This study investigated the apparent genetic redundancy in the biosynthesis of polyhydroxyalkanoates (PHAs) in the Rhodospirillum rubrum genome revealed by the occurrence of three homologous PHA polymerase genes (phaC1, phaC2, and phaC3). In vitro biochemical assays established that each gene product encodes PHA polymerase. A series of single, double, and triple phaC deletion mutants were characterized with respect to PHA production and growth capabilities on acetate or hexanoate as the sole carbon source. These analyses establish that phaC2 contributes the major capacity to produce PHA, even though the PhaC2 protein is not the most efficient PHA polymerase biocatalyst. In contrast, phaC3 is an insignificant contributor to PHA productivity, and phaC1, the PHA polymerase situated in the PHA biosynthetic operon, plays a minor role in this capability, even though both of these genes encode PHA polymerases that are more efficient enzymes. These observations are consistent with the finding that PhaC1 and PhaC3 occur at undetectable levels, at least 10-fold lower than that of PhaC2. The monomers in the PHA polymer produced by these strains establish that PhaC2 is responsible for the incorporation of the C(5) and C(6) monomers. The in vitro characterizations indicate that heteromeric PHA polymerases composed of mixtures of different PhaC paralogs are more efficient catalysts, suggesting that these proteins form complexes. Finally, the physiological role of PHA accumulation in enhancing the fitness of R. rubrum was indicated by the relationship between PHA content and growth capabilities of the genetically manipulated strains that express different levels of the PHA polymer.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Jin H,Nikolau BJdoi
10.1128/JB.01111-12subject
Has Abstractpub_date
2012-10-01 00:00:00pages
5522-9issue
20eissn
0021-9193issn
1098-5530pii
JB.01111-12journal_volume
194pub_type
杂志文章abstract::The chiR gene of Serratia marcescens 2170, encoding a LysR-type transcriptional activator, was identified previously as an essential factor for expression of chitinases and a chitin-binding protein, CBP21. To identify other genes that are essential for chitinase production, transposon mutagenesis with mini-Tn5Km1 was ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.6.1776-1782.2003
更新日期:2003-03-01 00:00:00
abstract::Ben-Hamida, Fakher (Washington University School of Medicine, St. Louis, Mo.), and David Schlessinger. Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli. J. Bacteriol. 90:1611-1616. 1965.-Synthesis of beta-galactosidase stops within several minutes when preinduced, permeaseless cultures ar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.90.6.1611-1616.1965
更新日期:1965-12-01 00:00:00
abstract::Bdellovibrio bacteriovorus is an obligate predatory bacterium that invades and kills a broad range of Gram-negative prey cells, including human pathogens. Its potential therapeutic application has been the subject of increased research interest in recent years. However, an improved understanding of the fundamental mol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00150-20
更新日期:2020-08-25 00:00:00
abstract::A computer program has been designed to study behavior in populations of Spirochaeta aurantia cells, and this program has been used to analyze changes in behavior in response to chemoattractants. Three kinds of behavior were distinguished: smooth swimming, flexing, and reversals in direction of swimming after a short ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1768-1774.1988
更新日期:1988-04-01 00:00:00
abstract::A locus unlinked to either katE or katF that affected catalase levels in Escherichia coli was identified and localized between metB and ppc at 89.2 min on the genome. The locus was named katG. Mutations in katG which prevented the formation of both isoenzyme forms of the bifunctional catalase-peroxidase HPI were creat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.2.661-667.1985
更新日期:1985-05-01 00:00:00
abstract::Cerulenin is a fungal mycotoxin that potently inhibits fatty acid synthesis by covalent modification of the active site thiol of the chain-elongation subtypes of beta-ketoacyl-acyl carrier protein (ACP) synthases. The Bacillus subtilis fabF (yjaY) gene (fabF(b)) encodes an enzyme that catalyzes the condensation of mal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.10.3032-3040.2001
更新日期:2001-05-01 00:00:00
abstract::We have mapped a regulatory site mediating the hyperproduction of cholera toxin in mutants of Vibrio cholerae strain 569B. Mutations in this locus, called htx, result in the hypertoxinogenic phenotype, as measured by the ganglioside filter assay and immunoradial diffusion. Transposon-facilitated recombination was used...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.139.3.859-865.1979
更新日期:1979-09-01 00:00:00
abstract::Fowler, Audree V. (University of California, Los Angeles), and Irving Zabin. Effects of dimethylsulfoxide on the lactose operon in Escherichia coli. J. Bacteriol. 92:353-357. 1966.-Dimethylsulfoxide (DMSO) at a concentration of 5% (v/v) in the culture medium inhibits the growth of Escherichia coli to only a slight ext...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.92.2.353-357.1966
更新日期:1966-08-01 00:00:00
abstract::Bacillus subtilis has developed an intricate signal transduction cascade to respond to the imposition of a variety of stresses on the cell. Reversible protein phosphorylation and the formation of alternative protein-protein complexes modulate the activity of sigma(B), the RNA polymerase sigma factor subunit responsibl...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00892-06
更新日期:2006-11-01 00:00:00
abstract::The lic3 locus of Haemophilus influenzae consists of four open reading frames. The derived amino acid sequences of orf2 and orf4 exhibit homology to Escherichia coli GalE and AdK, respectively. The functions of orf1 and orf3 remain unknown. orf1 contains multiple tandem repeats of the tetrameric DNA sequence CAAT near...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.22.7245-7252.1992
更新日期:1992-11-01 00:00:00
abstract::CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow on CO as a sole energy source. We have identified a number of CooA homologs through database searches, expressed these heterologously in Escherichia coli, and monitored their ability to respond to CO in vivo. Furth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.5.1320-1329.2004
更新日期:2004-03-01 00:00:00
abstract::Lysogenization of Salmonella typhimurium with either of the bacteriophages A3 and A4 results in O-acetylation of the L-rhamnose residues of the O-polysaccharide chain of the lipopolysaccharide of the bacterial cell envelope. The O-acetyl group is found on both O-2 and O-3 of the L-rhamnosyl residues. This lysogenic co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.3.1003-1009.1987
更新日期:1987-03-01 00:00:00
abstract::A method was developed for the isolation of Neurospora bradytrophs. The bradytrophs (representing lesions in a number of pathways) were resistant to DL-p-fluorophenylalanine when growing in a leaky fashion but were sensitive when grown in the presence of their stimulating supplement. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.1133-1136.1979
更新日期:1979-12-01 00:00:00
abstract::The catalase gene, katA, of the sepiolid squid symbiont Vibrio fischeri has been cloned and sequenced. The predicted amino acid sequence of KatA has a high degree of similarity to the recently defined group III catalases, including those found in Haemophilus influenzae, Bacteroides fragilis, and Proteus mirabilis. Ups...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.8.2087-2092.1998
更新日期:1998-04-01 00:00:00
abstract::Three spontaneous derivatives of Streptococcus faecium ATCC 9790, originally isolated as conditionally Triton X-100 detergent-resistant at 25 degrees C, displayed normal penicillin-induced rates of lysis at 37 degrees C but substantially reduced rates of lysis and killing at 25 degrees C. The addition of exogenous uns...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.805-807.1984
更新日期:1984-08-01 00:00:00
abstract::Carere, A., and Isabella Spada-Sermonti (Istituto Superiore di Sanità, Rome, Italy). Nutritional mutations and transduction by ultraviolet-inactivated phage in Staphylococcus aureus. J. Bacteriol. 88:226-232. 1964.-By use of a semisynthetic medium, various nutritional mutants of Staphylococcus aureus 80 were isolated....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.226-232.1964
更新日期:1964-07-01 00:00:00
abstract::The KRE1 gene of Saccharomyces cerevisiae, sacKRE1, appears to be involved in the synthesis of cell wall beta-glucan. S. cerevisiae strains with mutations in the KRE1 gene produce a structurally altered cell wall (1----6)-beta-glucan, which results in resistance to K1 killer toxin. We isolated the canKRE1 gene from Ca...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.21.6859-6864.1991
更新日期:1991-11-01 00:00:00
abstract::The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.17.5585-5594.1993
更新日期:1993-09-01 00:00:00
abstract::Silicibacter sp. TM1040 is a member of the marine Roseobacter clade of Alphaproteobacteria that forms symbioses with unicellular eukaryotic phytoplankton, such as dinoflagellates. The symbiosis is complex and involves a series of steps that physiologically change highly motile bacteria into cells that readily form bio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00429-09
更新日期:2009-07-01 00:00:00
abstract::We have constructed Escherichia coli strains containing mutations at two different loci, both originally selected for failure to support lambda site-specific recombination: himA and gyrB-him(Ts). Although the gyrB-him(Ts) mutations by themselves reduce supercoiling at high temperature, the double mutants show a far gr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.157.2.484-489.1984
更新日期:1984-02-01 00:00:00
abstract::Virulence in Shigella spp., as well as in strains of enteroinvasive Escherichia coli, is regulated by growth temperature. Previously, virR had been identified as the gene controlling the temperature-regulated expression of Shigella virulence. Since Shigella spp. and E. coli are also known to share greater than 90% DNA...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.5.2879-2881.1989
更新日期:1989-05-01 00:00:00
abstract::Acholeplasma laidlawii cells grown with oleic acid produced much more colored carotenoids than did cells grown with elaidic acid. The amount of carotenoids was decreased 80 to 90% by growing the cells with 0.05 M propionate, resulting in a marked increase in the mobility of both 5-doxylstearate and 12-doxylstearate in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.3.944-948.1979
更新日期:1979-12-01 00:00:00
abstract::Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.7.2078-2086.1992
更新日期:1992-04-01 00:00:00
abstract::Pathways of mannitol biosynthesis and utilization in Aspergillus candidus NRRL 305 were studied in cell-free extracts of washed mycelia prepared by sonic and French pressure cell treatments. A nicotinamide adenine dinucleotide-linked mannitol-1-phosphate (M1P) dehydrogenase was found in French pressure cell extracts o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.3.1305-1309.1969
更新日期:1969-03-01 00:00:00
abstract::Purified L-asparaginase from Serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. A subunit molecular weight of 31,500 +/- 1,500 was estimated for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.2.719-727.1976
更新日期:1976-02-01 00:00:00
abstract::Chemotactic transducer proteins of Escherichia coli contain four or five methyl-accepting glutamates that are crucial for sensory adaptation and gradient sensing. Two residues arise from posttranslational deamidation of glutamines to yield methyl-accepting glutamates. We addressed the significance of this arrangement ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.12.7179-7187.1990
更新日期:1990-12-01 00:00:00
abstract::The uhp locus of Escherichia coli contains genes for the sugar phosphate transport system (uhpT) and the regulatory system which allows its induction by external glucose 6-phosphate (uhpRA). The uhp region was cloned onto high-copy-number plasmids, both from Uhp(+) plasmids of the Clarke-Carbon collection and from gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.155.3.1062-1070.1983
更新日期:1983-09-01 00:00:00
abstract::Analysis of the Escherichia coli K10 capsule gene cluster identified two regions, regions 1 and 3, conserved between different group III capsule gene clusters. Region 1 encodes homologues of KpsD, KpsM, KpsT, and KpsE proteins, and region 3 encodes homologues of the KpsC and KpsS proteins. An rfaH mutation abolished K...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.7.2279-2285.1999
更新日期:1999-04-01 00:00:00
abstract::The ompR and envZ genes, which together constitute the ompB operon, are involved in osmoregulatory expression of the OmpF and OmpC proteins, major outer membrane proteins of Escherichia coli. The envZ11 mutation results in the OmpF- OmpC-constitutive phenotype. A mutant which suppressed defects caused by the envZ11 mu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.3.1309-1314.1986
更新日期:1986-12-01 00:00:00
abstract::The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was i...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.8.3359-3366.1988
更新日期:1988-08-01 00:00:00