Role of genetic redundancy in polyhydroxyalkanoate (PHA) polymerases in PHA biosynthesis in Rhodospirillum rubrum.

Abstract:

:This study investigated the apparent genetic redundancy in the biosynthesis of polyhydroxyalkanoates (PHAs) in the Rhodospirillum rubrum genome revealed by the occurrence of three homologous PHA polymerase genes (phaC1, phaC2, and phaC3). In vitro biochemical assays established that each gene product encodes PHA polymerase. A series of single, double, and triple phaC deletion mutants were characterized with respect to PHA production and growth capabilities on acetate or hexanoate as the sole carbon source. These analyses establish that phaC2 contributes the major capacity to produce PHA, even though the PhaC2 protein is not the most efficient PHA polymerase biocatalyst. In contrast, phaC3 is an insignificant contributor to PHA productivity, and phaC1, the PHA polymerase situated in the PHA biosynthetic operon, plays a minor role in this capability, even though both of these genes encode PHA polymerases that are more efficient enzymes. These observations are consistent with the finding that PhaC1 and PhaC3 occur at undetectable levels, at least 10-fold lower than that of PhaC2. The monomers in the PHA polymer produced by these strains establish that PhaC2 is responsible for the incorporation of the C(5) and C(6) monomers. The in vitro characterizations indicate that heteromeric PHA polymerases composed of mixtures of different PhaC paralogs are more efficient catalysts, suggesting that these proteins form complexes. Finally, the physiological role of PHA accumulation in enhancing the fitness of R. rubrum was indicated by the relationship between PHA content and growth capabilities of the genetically manipulated strains that express different levels of the PHA polymer.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Jin H,Nikolau BJ

doi

10.1128/JB.01111-12

subject

Has Abstract

pub_date

2012-10-01 00:00:00

pages

5522-9

issue

20

eissn

0021-9193

issn

1098-5530

pii

JB.01111-12

journal_volume

194

pub_type

杂志文章
  • Uptake of N,N'-diacetylchitobiose [(GlcNAc)2] via the phosphotransferase system is essential for chitinase production by Serratia marcescens 2170.

    abstract::The chiR gene of Serratia marcescens 2170, encoding a LysR-type transcriptional activator, was identified previously as an essential factor for expression of chitinases and a chitin-binding protein, CBP21. To identify other genes that are essential for chitinase production, transposon mutagenesis with mini-Tn5Km1 was ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.6.1776-1782.2003

    authors: Uchiyama T,Kaneko R,Yamaguchi J,Inoue A,Yanagida T,Nikaidou N,Regue M,Watanabe T

    更新日期:2003-03-01 00:00:00

  • Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli.

    abstract::Ben-Hamida, Fakher (Washington University School of Medicine, St. Louis, Mo.), and David Schlessinger. Stability of beta-galactosidase messenger ribonucleic acid in Escherichia coli. J. Bacteriol. 90:1611-1616. 1965.-Synthesis of beta-galactosidase stops within several minutes when preinduced, permeaseless cultures ar...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.6.1611-1616.1965

    authors: Ben-Hamida F,Schlessinger D

    更新日期:1965-12-01 00:00:00

  • Studies on Bd0934 and Bd3507, Two Secreted Nucleases from Bdellovibrio bacteriovorus, Reveal Sequential Release of Nucleases during the Predatory Cycle.

    abstract::Bdellovibrio bacteriovorus is an obligate predatory bacterium that invades and kills a broad range of Gram-negative prey cells, including human pathogens. Its potential therapeutic application has been the subject of increased research interest in recent years. However, an improved understanding of the fundamental mol...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00150-20

    authors: Bukowska-Faniband E,Andersson T,Lood R

    更新日期:2020-08-25 00:00:00

  • Motility and chemotaxis of Spirochaeta aurantia: computer-assisted motion analysis.

    abstract::A computer program has been designed to study behavior in populations of Spirochaeta aurantia cells, and this program has been used to analyze changes in behavior in response to chemoattractants. Three kinds of behavior were distinguished: smooth swimming, flexing, and reversals in direction of swimming after a short ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.4.1768-1774.1988

    authors: Fosnaugh K,Greenberg EP

    更新日期:1988-04-01 00:00:00

  • Genetic mapping of katG, a locus that affects synthesis of the bifunctional catalase-peroxidase hydroperoxidase I in Escherichia coli.

    abstract::A locus unlinked to either katE or katF that affected catalase levels in Escherichia coli was identified and localized between metB and ppc at 89.2 min on the genome. The locus was named katG. Mutations in katG which prevented the formation of both isoenzyme forms of the bifunctional catalase-peroxidase HPI were creat...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.162.2.661-667.1985

    authors: Loewen PC,Triggs BL,George CS,Hrabarchuk BE

    更新日期:1985-05-01 00:00:00

  • Response of Bacillus subtilis to cerulenin and acquisition of resistance.

    abstract::Cerulenin is a fungal mycotoxin that potently inhibits fatty acid synthesis by covalent modification of the active site thiol of the chain-elongation subtypes of beta-ketoacyl-acyl carrier protein (ACP) synthases. The Bacillus subtilis fabF (yjaY) gene (fabF(b)) encodes an enzyme that catalyzes the condensation of mal...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.183.10.3032-3040.2001

    authors: Schujman GE,Choi KH,Altabe S,Rock CO,de Mendoza D

    更新日期:2001-05-01 00:00:00

  • Genetic mapping of toxin regulatory mutations in Vibrio cholerae.

    abstract::We have mapped a regulatory site mediating the hyperproduction of cholera toxin in mutants of Vibrio cholerae strain 569B. Mutations in this locus, called htx, result in the hypertoxinogenic phenotype, as measured by the ganglioside filter assay and immunoradial diffusion. Transposon-facilitated recombination was used...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.139.3.859-865.1979

    authors: Mekalanos JJ,Sublett RD,Romig WR

    更新日期:1979-09-01 00:00:00

  • Effects of Dimethylsulfoxide on the Lactose Operon in Escherichia coli.

    abstract::Fowler, Audree V. (University of California, Los Angeles), and Irving Zabin. Effects of dimethylsulfoxide on the lactose operon in Escherichia coli. J. Bacteriol. 92:353-357. 1966.-Dimethylsulfoxide (DMSO) at a concentration of 5% (v/v) in the culture medium inhibits the growth of Escherichia coli to only a slight ext...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.92.2.353-357.1966

    authors: Fowler AV,Zabin I

    更新日期:1966-08-01 00:00:00

  • High-molecular-weight complexes of RsbR and paralogues in the environmental signaling pathway of Bacillus subtilis.

    abstract::Bacillus subtilis has developed an intricate signal transduction cascade to respond to the imposition of a variety of stresses on the cell. Reversible protein phosphorylation and the formation of alternative protein-protein complexes modulate the activity of sigma(B), the RNA polymerase sigma factor subunit responsibl...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00892-06

    authors: Delumeau O,Chen CC,Murray JW,Yudkin MD,Lewis RJ

    更新日期:2006-11-01 00:00:00

  • Use of chromosomal gene fusions to investigate the role of repetitive DNA in regulation of genes involved in lipopolysaccharide biosynthesis in Haemophilus influenzae.

    abstract::The lic3 locus of Haemophilus influenzae consists of four open reading frames. The derived amino acid sequences of orf2 and orf4 exhibit homology to Escherichia coli GalE and AdK, respectively. The functions of orf1 and orf3 remain unknown. orf1 contains multiple tandem repeats of the tetrameric DNA sequence CAAT near...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.22.7245-7252.1992

    authors: Szabo M,Maskell D,Butler P,Love J,Moxon R

    更新日期:1992-11-01 00:00:00

  • Functionally critical elements of CooA-related CO sensors.

    abstract::CooA is a heme-containing transcriptional activator that enables Rhodospirillum rubrum to sense and grow on CO as a sole energy source. We have identified a number of CooA homologs through database searches, expressed these heterologously in Escherichia coli, and monitored their ability to respond to CO in vivo. Furth...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.186.5.1320-1329.2004

    authors: Youn H,Kerby RL,Conrad M,Roberts GP

    更新日期:2004-03-01 00:00:00

  • Lysogenic conversion of Salmonella typhimurium bacteriophages A3 and A4 consists of O-acetylation of rhamnose of the repeating unit of the O-antigenic polysaccharide chain.

    abstract::Lysogenization of Salmonella typhimurium with either of the bacteriophages A3 and A4 results in O-acetylation of the L-rhamnose residues of the O-polysaccharide chain of the lipopolysaccharide of the bacterial cell envelope. The O-acetyl group is found on both O-2 and O-3 of the L-rhamnosyl residues. This lysogenic co...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.3.1003-1009.1987

    authors: Wollin R,Stocker BA,Lindberg AA

    更新日期:1987-03-01 00:00:00

  • Isolation of Neurospora crassa bradytrophs.

    abstract::A method was developed for the isolation of Neurospora bradytrophs. The bradytrophs (representing lesions in a number of pathways) were resistant to DL-p-fluorophenylalanine when growing in a leaky fashion but were sensitive when grown in the presence of their stimulating supplement. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.1133-1136.1979

    authors: Kinsey JA

    更新日期:1979-12-01 00:00:00

  • The periplasmic, group III catalase of Vibrio fischeri is required for normal symbiotic competence and is induced both by oxidative stress and by approach to stationary phase.

    abstract::The catalase gene, katA, of the sepiolid squid symbiont Vibrio fischeri has been cloned and sequenced. The predicted amino acid sequence of KatA has a high degree of similarity to the recently defined group III catalases, including those found in Haemophilus influenzae, Bacteroides fragilis, and Proteus mirabilis. Ups...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.8.2087-2092.1998

    authors: Visick KL,Ruby EG

    更新日期:1998-04-01 00:00:00

  • Detergent-resistant Streptococcus faecium derivatives that display conditional penicillin lysis.

    abstract::Three spontaneous derivatives of Streptococcus faecium ATCC 9790, originally isolated as conditionally Triton X-100 detergent-resistant at 25 degrees C, displayed normal penicillin-induced rates of lysis at 37 degrees C but substantially reduced rates of lysis and killing at 25 degrees C. The addition of exogenous uns...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.2.805-807.1984

    authors: Pucci MJ,Daneo-Moore L

    更新日期:1984-08-01 00:00:00

  • NUTRITIONAL MUTATIONS AND TRANSDUCTION BY ULTRAVIOLET-INACTIVATED PHAGE IN STAPHYLOCOCCUS AUREUS.

    abstract::Carere, A., and Isabella Spada-Sermonti (Istituto Superiore di Sanità, Rome, Italy). Nutritional mutations and transduction by ultraviolet-inactivated phage in Staphylococcus aureus. J. Bacteriol. 88:226-232. 1964.-By use of a semisynthetic medium, various nutritional mutants of Staphylococcus aureus 80 were isolated....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.88.1.226-232.1964

    authors: CARERE A,SPADA-SERMONTI I

    更新日期:1964-07-01 00:00:00

  • Isolation from Candida albicans of a functional homolog of the Saccharomyces cerevisiae KRE1 gene, which is involved in cell wall beta-glucan synthesis.

    abstract::The KRE1 gene of Saccharomyces cerevisiae, sacKRE1, appears to be involved in the synthesis of cell wall beta-glucan. S. cerevisiae strains with mutations in the KRE1 gene produce a structurally altered cell wall (1----6)-beta-glucan, which results in resistance to K1 killer toxin. We isolated the canKRE1 gene from Ca...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.173.21.6859-6864.1991

    authors: Boone C,Sdicu A,Laroche M,Bussey H

    更新日期:1991-11-01 00:00:00

  • Sequencing and characterization of a gene cluster encoding the enzymes for L-rhamnose metabolism in Escherichia coli.

    abstract::The sequencing of the EcoRI-HindIII fragment complementing mutations in the structural genes of the L-rhamnose regulon of Escherichia coli has permitted identification of the open reading frames corresponding to rhaB, rhaA, and rhaD. The deduced amino acid sequences gave a 425-amino-acid polypeptide corresponding to r...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.17.5585-5594.1993

    authors: Moralejo P,Egan SM,Hidalgo E,Aguilar J

    更新日期:1993-09-01 00:00:00

  • Genetic determinants of Silicibacter sp. TM1040 motility.

    abstract::Silicibacter sp. TM1040 is a member of the marine Roseobacter clade of Alphaproteobacteria that forms symbioses with unicellular eukaryotic phytoplankton, such as dinoflagellates. The symbiosis is complex and involves a series of steps that physiologically change highly motile bacteria into cells that readily form bio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00429-09

    authors: Belas R,Horikawa E,Aizawa S,Suvanasuthi R

    更新日期:2009-07-01 00:00:00

  • Synergistic effect of himA and gyrB mutations: evidence that him functions control expression of ilv and xyl genes.

    abstract::We have constructed Escherichia coli strains containing mutations at two different loci, both originally selected for failure to support lambda site-specific recombination: himA and gyrB-him(Ts). Although the gyrB-him(Ts) mutations by themselves reduce supercoiling at high temperature, the double mutants show a far gr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.2.484-489.1984

    authors: Friedman DI,Olson EJ,Carver D,Gellert M

    更新日期:1984-02-01 00:00:00

  • Identification of an Escherichia coli gene homologous to virR, a regulator of Shigella virulence.

    abstract::Virulence in Shigella spp., as well as in strains of enteroinvasive Escherichia coli, is regulated by growth temperature. Previously, virR had been identified as the gene controlling the temperature-regulated expression of Shigella virulence. Since Shigella spp. and E. coli are also known to share greater than 90% DNA...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.5.2879-2881.1989

    authors: Hromockyj AE,Maurelli AT

    更新日期:1989-05-01 00:00:00

  • Carotenoids acts as reinforcers of the Acholeplasma laidlawii lipid bilayer.

    abstract::Acholeplasma laidlawii cells grown with oleic acid produced much more colored carotenoids than did cells grown with elaidic acid. The amount of carotenoids was decreased 80 to 90% by growing the cells with 0.05 M propionate, resulting in a marked increase in the mobility of both 5-doxylstearate and 12-doxylstearate in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.140.3.944-948.1979

    authors: Rottem S,Markowitz O

    更新日期:1979-12-01 00:00:00

  • Determination of genome size, macrorestriction pattern polymorphism, and nonpigmentation-specific deletion in Yersinia pestis by pulsed-field gel electrophoresis.

    abstract::Of 16 restriction endonucleases known to hydrolyze rare 6- or 8-base recognition sequences that were tested, only SpeI, NotI, AscI, and SfiI generated fragments of chromosomal DNA from Yersinia pestis, the causative agent of bubonic plague, of sufficient length to permit physical analysis by pulsed-field gel electroph...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.7.2078-2086.1992

    authors: Lucier TS,Brubaker RR

    更新日期:1992-04-01 00:00:00

  • D-mannitol metabolism by Aspergillus candidus.

    abstract::Pathways of mannitol biosynthesis and utilization in Aspergillus candidus NRRL 305 were studied in cell-free extracts of washed mycelia prepared by sonic and French pressure cell treatments. A nicotinamide adenine dinucleotide-linked mannitol-1-phosphate (M1P) dehydrogenase was found in French pressure cell extracts o...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.3.1305-1309.1969

    authors: Strandberg GW

    更新日期:1969-03-01 00:00:00

  • Physical properties of L-asparaginase from Serratia marcescens.

    abstract::Purified L-asparaginase from Serratia marcescens had an apparent-weight average molecular weight of 171,000 to 180,000 as determined by electrophoresis on polyacrylamide gels and by sedimentation equilibrium at low speed in an analytical ultracentrifuge. A subunit molecular weight of 31,500 +/- 1,500 was estimated for...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.125.2.719-727.1976

    authors: Stern ML,Phillips AW,Gottlieb AJ

    更新日期:1976-02-01 00:00:00

  • Effects of glutamines and glutamates at sites of covalent modification of a methyl-accepting transducer.

    abstract::Chemotactic transducer proteins of Escherichia coli contain four or five methyl-accepting glutamates that are crucial for sensory adaptation and gradient sensing. Two residues arise from posttranslational deamidation of glutamines to yield methyl-accepting glutamates. We addressed the significance of this arrangement ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.12.7179-7187.1990

    authors: Park C,Dutton DP,Hazelbauer GL

    更新日期:1990-12-01 00:00:00

  • Molecular cloning of the uhp region and evidence for a positive activator for expression of the hexose phosphate transport system of Escherichia coli.

    abstract::The uhp locus of Escherichia coli contains genes for the sugar phosphate transport system (uhpT) and the regulatory system which allows its induction by external glucose 6-phosphate (uhpRA). The uhp region was cloned onto high-copy-number plasmids, both from Uhp(+) plasmids of the Clarke-Carbon collection and from gen...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.155.3.1062-1070.1983

    authors: Shattuck-Eidens DM,Kadner RJ

    更新日期:1983-09-01 00:00:00

  • Genetic organization of the Escherichia coli K10 capsule gene cluster: identification and characterization of two conserved regions in group III capsule gene clusters encoding polysaccharide transport functions.

    abstract::Analysis of the Escherichia coli K10 capsule gene cluster identified two regions, regions 1 and 3, conserved between different group III capsule gene clusters. Region 1 encodes homologues of KpsD, KpsM, KpsT, and KpsE proteins, and region 3 encodes homologues of the KpsC and KpsS proteins. An rfaH mutation abolished K...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.181.7.2279-2285.1999

    authors: Clarke BR,Pearce R,Roberts IS

    更新日期:1999-04-01 00:00:00

  • Interaction between two regulatory proteins in osmoregulatory expression of ompF and ompC genes in Escherichia coli: a novel ompR mutation suppresses pleiotropic defects caused by an envZ mutation.

    abstract::The ompR and envZ genes, which together constitute the ompB operon, are involved in osmoregulatory expression of the OmpF and OmpC proteins, major outer membrane proteins of Escherichia coli. The envZ11 mutation results in the OmpF- OmpC-constitutive phenotype. A mutant which suppressed defects caused by the envZ11 mu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.3.1309-1314.1986

    authors: Matsuyama S,Mizuno T,Mizushima S

    更新日期:1986-12-01 00:00:00

  • Excision repair of thymine glycols, urea residues, and apurinic sites in Escherichia coli.

    abstract::The genetic requirements for the excision repair of thymine glycols, urea residues, and apurinic (AP) sites were examined by measuring the survival in Escherichia coli mutants of phi X174 replicative form (RF) I transfecting DNA containing selectively introduced lesions. phi X RF I DNA containing thymine glycols was i...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.8.3359-3366.1988

    authors: Laspia MF,Wallace SS

    更新日期:1988-08-01 00:00:00