Abstract:
:Aspergillus fumigatus, an important opportunistic pathogen which commonly affects neutropenic patients, produces conidia with a bluish-green color. We identified a gene, alb1, which is required for conidial pigmentation. The alb1 gene encodes a putative polyketide synthase, and disruption of alb1 resulted in an albino conidial phenotype. Expression of alb1 is developmentally regulated, and the 7-kb transcript is detected only during the conidiation stage. The alb1 mutation was found to block 1,3,6,8-tetrahydroxynaphthalene production, indicating that alb1 is involved in dihydroxynaphthalene-melanin biosynthesis. Scanning electron microscopy studies showed that the alb1 disruptant exhibited a smooth conidial surface, whereas complementation of the alb1 deletion restored the echinulate wild-type surface. Disruption of alb1 resulted in a significant increase in C3 binding on conidial surfaces, and the conidia of the alb1 disruptant were ingested by human neutrophils at a higher rate than were those of the wild type. The alb1-complemented strain producing bluish-green conidia exhibited inefficient C3 binding and neutrophil-mediated phagocytosis quantitatively similar to those of the wild type. Importantly, the alb1 disruptant had a statistically significant loss of virulence compared to the wild-type and alb1-complemented strains in a murine model. These results suggest that disruption of alb1 causes pleiotropic effects on conidial morphology and fungal virulence.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Tsai HF,Chang YC,Washburn RG,Wheeler MH,Kwon-Chung KJdoi
10.1128/JB.180.12.3031-3038.1998subject
Has Abstractpub_date
1998-06-01 00:00:00pages
3031-8issue
12eissn
0021-9193issn
1098-5530journal_volume
180pub_type
杂志文章abstract::A handful of nucleoid-associated proteins (NAPs) regulate the vast majority of genes in a bacterial cell. H-NS, the histone-like nucleoid-structuring protein, is one of these NAPs and protects Escherichia coli from foreign gene expression. Though lacking any sequence similarity with E. coli H-NS, Rv3852 was annotated ...
journal_title:Journal of bacteriology
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abstract::Seven mutants of Haemophilus influenzae were isolated by the criterion of sensitivity to ultraviolet (UV) inactivation of colony formation. These mutants and the wild type were characterized with regard to X-ray inactivation of colony formation, UV induction of division inhibition, the ability of the eight strains to ...
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abstract::The syrA and syrB genes involved in syringomycin production in Pseudomonas syringae pv. syringae B301D were identified from an EcoRI-pLAFR3 cosmid library and then physically and functionally analyzed in relation to plant pathogenicity. Homologous recombination of the genes required for syringomycin production from co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1970-08-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1979-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1987-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1990-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1967-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.4.1460-1468.1987
更新日期:1987-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1984-07-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.115.3.949-956.1973
更新日期:1973-09-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:1984-08-01 00:00:00
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更新日期:1998-11-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1978-09-01 00:00:00
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更新日期:2006-01-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1988-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1988-04-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2009-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1979-02-01 00:00:00
abstract::Cystic fibrosis (CF) patients are highly susceptible to chronic pulmonary disease caused by mucoid Pseudomonas aeruginosa strains that overproduce the exopolysaccharide alginate. We showed here that a mutation in zwf, encoding glucose-6-phosphate dehydrogenase (G6PDH), leads to a approximately 90% reduction in alginat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-06-01 00:00:00