Abstract:
:Features of the amino acid sequence of the TnaC nascent peptide are recognized by the translating ribosome. Recognition leads to tryptophan binding within the translating ribosome, inhibiting the termination of tnaC translation and preventing Rho-dependent transcription termination in the tna operon leader region. It was previously shown that inserting an adenine residue at position 751 or introducing the U2609C change in 23S rRNA or introducing the K90W replacement in ribosomal protein L22 prevented tryptophan induction of tna operon expression. It was also observed that an adenine at position 752 of 23S rRNA was required for induction. In the current study, the explanation for the lack of induction by these altered ribosomes was investigated. Using isolated TnaC-ribosome complexes, it was shown that although tryptophan inhibits puromycin cleavage of TnaC-tRNA(Pro) with wild-type ribosome complexes, it does not inhibit cleavage with the four mutant ribosome complexes examined. Similarly, tryptophan prevents sparsomycin inhibition of TnaC-tRNA(Pro) cleavage with wild-type ribosome complexes but not with these mutant ribosome complexes. Additionally, a nucleotide located close to the peptidyl transferase center, A2572, which was protected from methylation by tryptophan with wild-type ribosome complexes, was not protected with mutant ribosome complexes. These findings identify specific ribosomal residues located in the ribosome exit tunnel that recognize features of the TnaC peptide. This recognition creates a free tryptophan-binding site in the peptidyl transferase center, where bound tryptophan inhibits peptidyl transferase activity.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Cruz-Vera LR,New A,Squires C,Yanofsky Cdoi
10.1128/JB.01869-06subject
Has Abstractpub_date
2007-04-01 00:00:00pages
3140-6issue
8eissn
0021-9193issn
1098-5530pii
JB.01869-06journal_volume
189pub_type
杂志文章abstract::Certain carbohydrates (rhamnose, 3-O-methyl rhamnose, and galactosamine) have been demonstrated to be present in Bacillus anthracis spores but absent in vegetative cells. Others have demonstrated that these spore-specific sugars are constituents of the glycoprotein BclA. In the current work, spore extracts were separa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.187.13.4592-4597.2005
更新日期:2005-07-01 00:00:00
abstract::Freundlich, Martin (University of Minnesota, Minneapolis) and Herman C. Lichstein. Tryptophanase-tryptophan synthetase systems in Escherichia coli. III. Requirements for enzyme synthesis. J. Bacteriol. 84:996-1006. 1962.-The requirements for the formation of tryptophanase and tryptophan synthetase in Escherichia coli ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.5.996-1006.1962
更新日期:1962-11-01 00:00:00
abstract::In the fungus Podospora anserina, the interaction between the nonallelic incompatible R and V genes has two consequences: a lytic reaction due to the synthesis of specific proteolytic enzymes, and a quenching in protein and ribonucleic acid synthesis. The incompatibility reaction when vegetative or sexual R and V cell...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.120.2.854-860.1974
更新日期:1974-11-01 00:00:00
abstract::Alcaligenes sp. strain CC1 is able to grow on several alpha-chlorinated aliphatic acids (2-chlorobutyrate, 2-chloropropionate, and chloroacetate), as well as on the beta-chlorinated four-carbon aliphatic acids trans-3-chlorocrotonate, cis-3-chlorocrotonate, and 3-chlorobutyrate as sole carbon and energy sources. Dehal...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1428-1434.1989
更新日期:1989-03-01 00:00:00
abstract::Sixteen independent Azorhizobium sesbaniae ORS571 vector insertion (Vi) mutants defective in ammonium assimilation (Asm-) were selected; genomic DNA sequences flanking the insertion endpoints were cloned directly. Resulting recombinant plasmids were used to identify, by hybridization, corresponding wild-type DNA seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.3.1197-1204.1988
更新日期:1988-03-01 00:00:00
abstract::A primary alcohol dehydrogenase has been purified from Methylococcus capsulatus (Texas strain). The purified enzyme catalyzes the oxidation of methanol and formaldehyde to formate; other primary alcohols are oxidized to their corresponding aldehydes. Ammonium ions are required for enzyme activity. The enzyme has a mol...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.110.2.570-577.1972
更新日期:1972-05-01 00:00:00
abstract::Tracer studies of pyrimidine biosynthesis in Lactobacillus leichmannii (ATCC 7830) indicated that, while aspartate is utilized in the usual manner, the guanido carbon of arginine, rather than carbon dioxide, is utilized as a pyrimidine precursor. The guanido carbon of arginine also contributes, to some extent, to the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.96.4.1249-1254.1968
更新日期:1968-10-01 00:00:00
abstract::An investigation of sulfate reduction in B tsnC*7004, a mutant of Escherichia coli lacking thioredoxin, is reported. Although thioredoxin is indispensable for the adenosine 3'-phosphate 5'-phosphosulfate (PAPS) sulfotransferase reaction under the usual conditions of assay in extracts of wild-type cells, the mutant gre...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.1.131-138.1978
更新日期:1978-04-01 00:00:00
abstract::The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.5.1560-1566.1968
更新日期:1968-05-01 00:00:00
abstract::The expression of cell cycle events in Caulobacter crescentus CB13 has been shown to be associated with regulation of carbohydrate utilization. Growth on lactose and galactose depends on induction of specific enzymes. Prior growth on glucose results in a delay in enzyme expression and cell cycle arrest at the nonmotil...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.131.3.951-959.1977
更新日期:1977-09-01 00:00:00
abstract::The flavodoxins constitute a highly conserved family of small, acidic electron transfer proteins with flavin mononucleotide prosthetic groups. They are found in prokaryotes and in red and green algae, where they provide electrons at low potentials for the reduction of nitrogen by nitrogenase, for the light-dependent r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.5.1729-1737.1991
更新日期:1991-03-01 00:00:00
abstract::Mycoplasma penetrans is a newly identified species of the genus MYCOPLASMA: It was first isolated from a urine sample from a human immunodeficiency virus (HIV)-infected patient. M. penetrans changes its surface antigen profile with high frequency. The changes originate from ON<==>OFF phase variations of the P35 family...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.1.231-242.2003
更新日期:2003-01-01 00:00:00
abstract::Transcription of an aqpZ-lac fusion in a single copy on the Escherichia coli chromosome increased as cells entered the stationary growth phase. This was true in a variety of media, and increased transcription in enriched medium required the RpoS sigma factor. Expression of the aqpZ-lac fusion was not affected by up- o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.15.4304-4307.2002
更新日期:2002-08-01 00:00:00
abstract::Toxin and antitoxin (TA) gene pairs are addiction systems that are present in many microbial genomes. Sinorhizobium meliloti is an N2-fixing bacterial symbiont of alfalfa and other leguminous plants, and its genome consists of three large replicons, a circular chromosome (3.7 Mb) and the megaplasmids pSymA (1.4 Mb) an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01104-13
更新日期:2014-02-01 00:00:00
abstract::Coat-stripped spores suspended in hypertonic solutions and supplied with two essential cations can be converted into viable protoplasts by lysozyme digestion of both cortex and germ cell wall. Calcium ions are necessary to prevent membrane rupture, and magnesium ions are necessary for changes indicative of hydration o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.3.1119-1136.1971
更新日期:1971-03-01 00:00:00
abstract::A procedure is described for the purification of the cores of flagella sheared from Vibrio cholerae. V. cholerae is a monotrichous organism whose flagellar core (FC) is enclosed within a sheath. The purification procedure consists of several cycles of differential centrifugation and cesium chloride density-gradient ul...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.1121-1128.1977
更新日期:1977-02-01 00:00:00
abstract::A nitrate-utilizing strain of marine bacteria was isolated in which luciferase was inducible by l-arginine. The induction was highly specific; structural analogues of arginine were ineffective, as were other natural amino acids. Several metabolites of arginine acted as weak inducers but did not affect the rate of indu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1638-1647.1967
更新日期:1967-11-01 00:00:00
abstract::Microbacterium barkeri strain 2011-R4 is a Gram-positive epiphyte which has been confirmed as a biocontrol agent against several plant pathogens in our previous studies. Here, we present the draft genome sequence of this strain, which was isolated from the rice rhizosphere in Tonglu city, Zhejiang province, China. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01468-12
更新日期:2012-12-01 00:00:00
abstract::Pseudomonas aeruginosa is usually resistant to a wide variety of antibacterial agents, and it has been inferred, on the basis of indirect evidence, that this was due to the low permeability of its outer membrane. We determined the permeability of P. aeruginosa outer membrane directly, by measuring the rates of hydroly...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:
更新日期:1982-11-01 00:00:00
abstract::In Escherichia coli, repression of the aceBAK operon is mediated by the IclR protein. We used an in vitro oligonucleotide selection technique to determine the consensus recognition sequence for MR. Mutational analysis confirmed the contribution of this sequence to repression in vivo and identified the -35 element of t...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.13.3982-3984.1996
更新日期:1996-07-01 00:00:00
abstract::Three genes were shown to provide functions specific for ferric enterobactin transport in Escherichia coli: fepA encoded the outer membrane receptor, fepB produced a periplasmic protein, and the fepC product was presumably a component of a cytoplasmic membrane permease system for this siderophore. A 10.6-kilobase-pair...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.8.3638-3646.1987
更新日期:1987-08-01 00:00:00
abstract::Motility in mycobacteria was described for the first time in 1999. It was reported that Mycobacterium smegmatis and Mycobacterium avium could spread on the surface of solid growth medium by a sliding mechanism and that the presence of cell wall glycopeptidolipids was essential for motility. We recently reported that M...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00572-08
更新日期:2008-10-01 00:00:00
abstract::Stalk synthesis in Caulobacter crescentus is a developmentally controlled and spatially restricted event that requires the synthesis of peptidoglycan at the stalk-cell body junction. We show that the beta-lactam antibiotic mecillinam prevents stalk synthesis by inhibiting stalk elongation. In addition, mecillinam caus...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.19.5235-5239.1998
更新日期:1998-10-01 00:00:00
abstract::Molybdenum (Mo) is an important trace element that is toxic at high concentrations. To resolve the mechanisms underlying Mo toxicity, Rhodobacter capsulatus mutants tolerant to high Mo concentrations were isolated by random transposon Tn5 mutagenesis. The insertion sites of six independent isolates mapped within the s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00742-10
更新日期:2010-11-01 00:00:00
abstract::Alcaligenes eutrophus H16 produces two [NiFe] hydrogenases which catalyze the oxidation of hydrogen and enable the organism to utilize H2 as the sole energy source. The genes (hoxK and hoxG) for the heterodimeric, membrane-bound hydrogenase (MBH) are located adjacent to a series of eight accessory genes (hoxZ, hoxM, h...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.15.4522-4529.1996
更新日期:1996-08-01 00:00:00
abstract::Mutant strains of Escherichia coli K-12 were isolated possessing mutations which affected the tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase, the phenylalanine-inhibitable DAHP synthetase, or the tryptophan-repressible DAHP synthetase. The mutations causing the loss of each of th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.237-244.1967
更新日期:1967-01-01 00:00:00
abstract::Very little is known about the role of DNA repair networks in Brucella abortus and its role in pathogenesis. We investigated the roles of RecA protein, DNA repair, and SOS regulation in B. abortus. While recA mutants in most bacterial species are hypersensitive to UV damage, surprisingly a B. abortus recA null mutant ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01994-05
更新日期:2006-07-01 00:00:00
abstract::For the ornithine fermentation pathway, described more than 70 years ago, genetic and biochemical information are still incomplete. We present here the experimental identification of the last four missing genes of this metabolic pathway. They encode L-ornithine racemase, (2R,4S)-2,4-diaminopentanoate dehydrogenase, an...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01777-08
更新日期:2009-05-01 00:00:00
abstract::Firshein, W. (Wesleyan University, Middletown, Conn.). Effects of deoxyribonucleic acid products on deoxyribonucleic acid synthesis of virulent and avirulent pneumococci. J. Bacteriol. 82:169-180. 1961.-Cell suspensions of virulent pneumococci synthesize a greater amount of deoxyribonucleic acid (DNA) per cell in the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.2.169-180.1961
更新日期:1961-08-01 00:00:00
abstract::The phage 21 holin, S(21), forms small membrane holes that depolarize the membrane and is designated as a pinholin, as opposed to large-hole-forming holins, like S(lambda). Pinholins require secreted SAR endolysins, a pairing that may represent an intermediate in the evolution of canonical holin-endolysin systems. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00847-07
更新日期:2007-12-01 00:00:00