Abstract:
:Molybdenum (Mo) is an important trace element that is toxic at high concentrations. To resolve the mechanisms underlying Mo toxicity, Rhodobacter capsulatus mutants tolerant to high Mo concentrations were isolated by random transposon Tn5 mutagenesis. The insertion sites of six independent isolates mapped within the same gene predicted to code for a permease of unknown function located in the cytoplasmic membrane. During growth under Mo-replete conditions, the wild-type strain accumulated considerably more Mo than the permease mutant. For mutants defective for the permease, the high-affinity molybdate importer ModABC, or both transporters, in vivo Mo-dependent nitrogenase (Mo-nitrogenase) activities at different Mo concentrations suggested that ModABC and the permease import molybdate in nanomolar and micromolar ranges, respectively. Like the permease mutants, a mutant defective for ATP sulfurylase tolerated high Mo concentrations, suggesting that ATP sulfurylase is the main target of Mo inhibition in R. capsulatus. Sulfate-dependent growth of a double mutant defective for the permease and the high-affinity sulfate importer CysTWA was reduced compared to those of the single mutants, implying that the permease plays an important role in sulfate uptake. In addition, permease mutants tolerated higher tungstate and vanadate concentrations than the wild type, suggesting that the permease acts as a general oxyanion importer. We propose to call this permease PerO (for oxyanion permease). It is the first reported bacterial molybdate transporter outside the ABC transporter family.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Gisin J,Müller A,Pfänder Y,Leimkühler S,Narberhaus F,Masepohl Bdoi
10.1128/JB.00742-10subject
Has Abstractpub_date
2010-11-01 00:00:00pages
5943-52issue
22eissn
0021-9193issn
1098-5530pii
JB.00742-10journal_volume
192pub_type
杂志文章abstract::The Bacillus subtilis anti-TRAP protein regulates the ability of the tryptophan-activated TRAP protein to bind to trp operon leader RNA and promote transcription termination. AT synthesis is regulated both transcriptionally and translationally by uncharged tRNA(Trp). In this study, we examined the roles of AT synthesi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2005-10-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1975-08-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1982-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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pub_type: 杂志文章,评审
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更新日期:2016-01-01 00:00:00