Abstract:
:Germination of dormant Bacillus subtilis spores with specific nutrient germinants is dependent on a number of inner membrane (IM) proteins, including (i) the GerA, GerB, and GerK germinant receptors (GRs) that respond to nutrient germinants; (ii) the GerD protein, essential for optimal GR function; and (iii) SpoVA proteins, essential for the release of the spore-specific molecule dipicolinic acid (DPA) during spore germination. Levels of GR A and C subunit proteins, GerD, and SpoVAD in wild-type spores were determined by Western blot analysis of spore fractions or total disrupted spores by comparison with known amounts of purified proteins. Surprisingly, after disruption of decoated B. subtilis spores with lysozyme and fractionation, ∼90% of IM fatty acids and GR subunits remained with the spores' insoluble integument fraction, indicating that yields of purified IM are low. The total lysate from disrupted wild-type spores contained ∼2,500 total GRs/spore: GerAA and GerAC subunits each at ∼1,100 molecules/spore and GerBC and GerKA subunits each at ∼700 molecules/spore. Levels of the GerBA subunit determined previously were also predicted to be ∼700 molecules/spore. These results indicate that the A/C subunit stoichiometry in GRs is most likely 1:1, with GerA being the most abundant GR. GerD and SpoVAD levels were ∼3,500 and ∼6,500 molecules/spore, respectively. These values will be helpful in formulating mathematic models of spore germination kinetics as well as setting lower limits on the size of the GR-GerD complex in the spores' IM, termed the germinosome.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Stewart KA,Setlow Pdoi
10.1128/JB.00377-13subject
Has Abstractpub_date
2013-08-01 00:00:00pages
3575-82issue
16eissn
0021-9193issn
1098-5530pii
JB.00377-13journal_volume
195pub_type
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
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更新日期:1983-04-01 00:00:00
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更新日期:1992-11-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2003-03-01 00:00:00
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更新日期:2011-12-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/jb.171.2.1228-1232.1989
更新日期:1989-02-01 00:00:00
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pub_type: 杂志文章
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更新日期:1976-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.21.5803-5807.1998
更新日期:1998-11-01 00:00:00
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更新日期:1970-02-01 00:00:00