Sulfolobus acidocaldarius synthesizes UMP via a standard de novo pathway: results of biochemical-genetic study.

abstract：:Bacterial lipases play important roles in bacterial metabolism and environmental response. Our laboratory recently discovered that a novel lipoprotein lysophospholipase, VolA, localizes on the surface of the Gram-negative aquatic pathogen Vibrio cholerae. VolA functions to cleave exogenous lysophosphatidylcholine, fre...

journal_title：Journal of bacteriology

authors： Pride AC,Guan Z,Trent MS

更新日期：2014-04-01 00:00:00

abstract：:Bacterial lipoproteins represent a subset of membrane-associated proteins that are covalently modified with lipids at the N-terminal cysteine. The final step of lipoprotein modification, N-acylation of apolipoproteins, is mediated by apolipoprotein N-acyltransferase (Lnt). Examinations with reconstituted proteoliposom...

journal_title：Journal of bacteriology

authors： Narita S,Tokuda H

更新日期：2011-09-01 00:00:00

abstract：:SecA signal peptide interaction is critical for initiating protein translocation in the bacterial Sec-dependent pathway. Here, we have utilized the recent nuclear magnetic resonance (NMR) and Förster resonance energy transfer studies that mapped the location of the SecA signal peptide-binding site to design and isolat...

journal_title：Journal of bacteriology

authors： Grady LM,Michtavy J,Oliver DB

更新日期：2012-01-01 00:00:00

abstract：:A group of enzymes of sulfur metabolism (arylsulfatase, cholinesulfatase, and a number of others) are normally repressed in Neurospora crassa by an abundant supply of a "favored" sulfur source such as methionine or inorganic sulfate. A mutant called scon(c) was isolated in which the formation of each of these enzymes ...

journal_title：Journal of bacteriology

authors： Burton EG,Metzenberg RL

更新日期：1972-01-01 00:00:00

abstract：:Bishop, D. G. (University of Sydney, Sydney, Australia), and J. L. Still. Fatty acid metabolism in Serratia marcescens. J. Bacteriol. 82:370-375. 1961.-A study has been made of the oxidation of saturated fatty acids containing between two and 18 carbon atoms by whole cells of the bacterium, Serratia marcescens. This o...

journal_title：Journal of bacteriology

authors： Bishop DG,Still JL

更新日期：1961-09-01 00:00:00

abstract：:Six peptidase activities have been distinguished electrophoretically in cell extracts of Salmonella typhimurium with the aid of a histochemical stain. The activities can also be partially separated by chromatography on diethylaminoethyl-cellulose. These peptidases show overlapping substrate specificities. Mutants (pep...

journal_title：Journal of bacteriology

authors： Miller CG,Mackinnon K

更新日期：1974-10-01 00:00:00

abstract：:The fec region of the Escherichia coli chromosome determines a citrate-dependent iron(III) transport system. The nucleotide sequence of fec revealed five genes, fecABCDE, which are transcribed from fecA to fecE. The fecA gene encodes a previously described outer membrane receptor protein. The fecB gene product is form...

journal_title：Journal of bacteriology

authors： Staudenmaier H,Van Hove B,Yaraghi Z,Braun V

更新日期：1989-05-01 00:00:00

abstract：:We have cloned the palA gene of Aspergillus nidulans, one of six genes participating in ambient pH signal transduction in a regulatory circuit mediating pH regulation of gene expression. The derived 798-residue PalA protein is 29.4% identical over its entire length to a hypothetical protein from the nematode Caenorhab...

journal_title：Journal of bacteriology

authors： Negrete-Urtasun S,Denison SH,Arst HN Jr

更新日期：1997-03-01 00:00:00

abstract：:Cells of Bacillus thuringiensis containing refractile spores autolyzed readily when suspended in buffer. The autolysate contained enzymes which lysed vegetative cell walls of the organism. Three enzymes were isolated from the autolysate, and each was purified approximately 30-fold. One enzyme, most active near pH 4.0,...

journal_title：Journal of bacteriology

authors： Kingan SL,Ensign JC

更新日期：1968-09-01 00:00:00

abstract：:An open-reading-frame fragment of a Methylobacterium sp. strain AM1 gene (moxF) encoding a portion of the methanol dehydrogenase structural protein has been used as a hybridization probe to detect similar sequences in a variety of methylotrophic bacteria. This hybridization was used to isolate clones containing putati...

journal_title：Journal of bacteriology

authors： Stephens RL,Haygood MG,Lidstrom ME

更新日期：1988-05-01 00:00:00

abstract：:The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell inv...

journal_title：Journal of bacteriology

authors： Pulkkinen WS,Miller SI

更新日期：1991-01-01 00:00:00

abstract：:Staphylococcus aureus responds with protective strategies against phagocyte-derived reactive oxidants to infect humans. Herein, we report the transcriptome analysis of the cellular response of S. aureus to hydrogen peroxide-induced oxidative stress. The data indicate that the oxidative response includes the induction ...

journal_title：Journal of bacteriology

authors： Chang W,Small DA,Toghrol F,Bentley WE

更新日期：2006-02-01 00:00:00

abstract：:The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...

journal_title：Journal of bacteriology

authors： Jin S,Roitsch T,Ankenbauer RG,Gordon MP,Nester EW

更新日期：1990-02-01 00:00:00

abstract：:Pseudomonas sp. strain PCL1171 displays colony phase variation between opaque phase I and translucent phase II colonies, thereby regulating the production of secondary metabolites and exoenzymes. Complementation and sequence analysis of 26 phase II mutants and of 13 wild-type phase II sectors growing out of phase I co...

journal_title：Journal of bacteriology

authors： van den Broek D,Chin-A-Woeng TF,Bloemberg GV,Lugtenberg BJ

更新日期：2005-01-01 00:00:00

abstract：:Cell walls (LOG walls) were isolated from cultures of Streptococcus faecalis ATCC 9790 in the exponential phase of growth. These walls were either allowed to undergo autolytic dissolution (in the presence or absence of trypsin) or wall autolysis was inactivated with sodium dodecylsulfate (SDS walls). Inactivated walls...

journal_title：Journal of bacteriology

authors： Shockman GD,Martin JT

更新日期：1968-11-01 00:00:00

abstract：:We provide experimental evidence for RNase III-dependent processing in helix 9 of the 23S rRNA as a general feature of many species in the alpha subclass of Proteobacteria (alpha-Proteobacteria). We investigated 12 Rhodobacter, Rhizobium, Sinorhizobium, Rhodopseudomonas, and Bartonella strains. The processed region is...

journal_title：Journal of bacteriology

authors： Evguenieva-Hackenberg E,Klug G

更新日期：2000-09-01 00:00:00

abstract：:Pseudomonas aeruginosa hemolytic phospholipase C (PlcH) degrades phosphatidylcholine (PC), an abundant lipid in cell membranes and lung surfactant. A ΔplcHR mutant, known to be defective in virulence in animal models, was less able to colonize epithelial cell monolayers and was defective in biofilm formation on plasti...

journal_title：Journal of bacteriology

authors： Jackson AA,Gross MJ,Daniels EF,Hampton TH,Hammond JH,Vallet-Gely I,Dove SL,Stanton BA,Hogan DA

更新日期：2013-07-01 00:00:00

abstract：:Although BBA74 initially was described as a 28-kDa virulence-associated outer-membrane-spanning protein with porin-like function, subsequent studies revealed that it is periplasmic and downregulated in mammalian host-adapted spirochetes. To further elucidate the role of this protein in the Borrelia burgdorferi tick-ma...

journal_title：Journal of bacteriology

authors： Mulay VB,Caimano MJ,Iyer R,Dunham-Ems S,Liveris D,Petzke MM,Schwartz I,Radolf JD

更新日期：2009-04-01 00:00:00

abstract：:Physiological studies of a mutant of Escherichia coli lacking the three lytic transglycosylases Slt70, MltA, and MltB revealed that interference with murein turnover can prevent AmpC beta-lactamase induction. The triple mutant, although growing normally, shows a dramatically reduced rate of murein turnover. Despite th...

journal_title：Journal of bacteriology

authors： Kraft AR,Prabhu J,Ursinus A,Höltje JV

更新日期：1999-12-01 00:00:00

abstract：:We used primer extension and mutational analysis to identify a promoter upstream of ilvB, the first gene in the ilv-leu operon of Bacillus subtilis. Between the promoter and ilvB, there is a 482-bp leader region which contains a sequence that resembles a factor-independent transcription terminator. In in vitro transcr...

journal_title：Journal of bacteriology

authors： Grandoni JA,Zahler SA,Calvo JM

更新日期：1992-05-01 00:00:00

abstract：:Escherichia coli ssrA encodes a small stable RNA molecule, tmRNA, that has many diverse functions, including tagging abnormal proteins for degradation, supporting phage growth, and modulating the activity of DNA binding proteins. Here we show that ssrA plays a role in Salmonella enterica serovar Typhimurium pathogenes...

journal_title：Journal of bacteriology

authors： Julio SM,Heithoff DM,Mahan MJ

更新日期：2000-03-01 00:00:00

abstract：:In Escherichia coli K-12, expression of the lysU gene is regulated by the lrp gene product, as indicated by an increase in the level of lysyl-tRNA synthetase activity and LysU protein in an lrp mutant. Comparison of the patterns of protein expression visualized by two-dimensional gel electrophoresis indicated that Lys...

journal_title：Journal of bacteriology

authors： Lin R,Ernsting B,Hirshfield IN,Matthews RG,Neidhardt FC,Clark RL,Newman EB

更新日期：1992-05-01 00:00:00

abstract：:Expression of the Vibrio vulnificus metalloprotease gene, vvp, was turned up rapidly when bacterial growth reached the late log phase. A similar pattern of expression has been found in the metalloprotease gene of Vibrio cholerae, and this has been shown to be regulated by a Vibrio harveyi LuxR-like transcriptional act...

journal_title：Journal of bacteriology

authors： Shao CP,Hor LI

更新日期：2001-02-01 00:00:00

abstract：:Six of the genes encoding enzymes of the beta-ketoadipate pathway for benzoate and 4-hydroxybenzoate degradation in Pseudomonas putida are organized into at least three separate transcriptional units. As an initial step to defining this pca regulon at the molecular level, lacZ fusions were made with the pcaI and pcaJ ...

journal_title：Journal of bacteriology

authors： Parales RE,Harwood CS

更新日期：1993-09-01 00:00:00

abstract：:In the fungus Podospora anserina, the interaction between the nonallelic incompatible R and V genes has two consequences: a lytic reaction due to the synthesis of specific proteolytic enzymes, and a quenching in protein and ribonucleic acid synthesis. The incompatibility reaction when vegetative or sexual R and V cell...

journal_title：Journal of bacteriology

authors： Labarére J,Bègueret J,Bernet J

更新日期：1974-11-01 00:00:00

abstract：:We developed a procedure for isolating membrane vesicles from the homolactic fermentative bacterium Streptococcus cremoris. The membrane vesicles were shown to have a right-side-out orientation by freeze-etch electron microscopy and to be free of cytoplasmic constituents. The membrane vesicles retained their functiona...

journal_title：Journal of bacteriology

authors： Otto R,Lageveen RG,Veldkamp H,Konings WN

更新日期：1982-02-01 00:00:00

abstract：:Addition of urea to an uninduced culture of Saccharomyces at 22 C results in appearance of allophanate hydrolase activity after a lag of 12 min. We have previously demonstrated that both ribonucleic acid (RNA) and protein synthesis are needed for this induction to occur. To elucidate the time intervals occupied by kno...

journal_title：Journal of bacteriology

authors： Bossinger J,Cooper TG

更新日期：1976-04-01 00:00:00

abstract：:The polycistronic mRNA encoding the nine genes of the unc operon of Escherichia coli was studied. We demonstrated the ribosome-binding capabilities of six of the nine unc genes, uncB, uncE, uncF, uncH, uncA, and uncD, by using the technique of primer extension inhibition or "toeprinting." No toeprint was detected for ...

journal_title：Journal of bacteriology

authors： Schaefer EM,Hartz D,Gold L,Simoni RD

更新日期：1989-07-01 00:00:00

abstract：:The sequential morphological events in spore formation by Clostridium perfringens type D were observed in Ellner's medium where 80 to 100% of the cells formed spores. Gross structural changes were studied with the light microscope under phase-contrast, and in fixed cells by the use of both nigrosin and Giemsa preparat...

journal_title：Journal of bacteriology

authors： Hoeniger JF,Stuart PF,Holt SC

更新日期：1968-11-01 00:00:00

abstract：:Listeria monocytogenes has caused numerous human outbreaks. Here we report draft genomes of L. monocytogenes J1816 and J1-220, which belong to epidemic clones II and IV, respectively. Whole-genome sequence analysis of these strains provides a tool for studying the short-term evolution of these epidemic clones. ...

journal_title：Journal of bacteriology

authors： Chen Y,Strain EA,Allard M,Brown EW

更新日期：2011-07-01 00:00:00