Abstract:
:The ESX-1 (ESAT-6 system 1) secretion system plays a conserved role in the virulence of diverse mycobacterial pathogens, including the human pathogen Mycobacterium tuberculosis and M. marinum, an environmental mycobacterial species. The ESX-1 system promotes the secretion of protein virulence factors to the extracytoplasmic environment. The secretion of these proteins triggers the host response by lysing the phagosome during macrophage infection. Using proteomic analyses of the M. marinum secretome in the presence and absence of a functional ESX-1 system, we and others have hypothesized that MMAR_2894, a PE family protein, is a potential ESX-1 substrate in M. marinum We used genetic and quantitative proteomic approaches to determine if MMAR_2894 is secreted by the ESX-1 system, and we defined the requirement of MMAR_2894 for ESX-1-mediated secretion and virulence. We show that MMAR_2894 is secreted by the ESX-1 system in M. marinum and is itself required for the optimal secretion of the known ESX-1 substrates in M. marinum Moreover, we found that MMAR_2894 was differentially required for hemolysis and cytolysis of macrophages, two lytic activities ascribed to the M. marinum ESX-1 system.IMPORTANCE Both Mycobacterium tuberculosis, the cause of human tuberculosis (TB), and Mycobacterium marinum, a pathogen of ectotherms, use the ESX-1 secretion system to cause disease. There are many established similarities between the ESX-1 systems in M. tuberculosis and in M. marinum Yet the two bacteria infect different hosts, hinting at species-specific functions of the ESX-1 system. Our findings demonstrate that MMAR_2894 is a PE protein secreted by the ESX-1 system of M. marinum We show that MMAR_2894 is required for the optimal secretion of mycobacterial proteins required for disease. Because the MMAR_2894 gene is not conserved in M. tuberculosis, our findings demonstrate that MMAR_2894 may contribute to a species-specific function of the ESX-1 system in M. marinum, providing new insight into how the M. marinum and M. tuberculosis systems differ.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Bosserman RE,Nicholson KR,Champion MM,Champion PAdoi
10.1128/JB.00760-18subject
Has Abstractpub_date
2019-06-21 00:00:00issue
14eissn
0021-9193issn
1098-5530pii
JB.00760-18journal_volume
201pub_type
杂志文章abstract::Procedures were developed to facilitate the identification of genes that belong to a given regulon and characterization of their responses to the regulator. The regulon controlled by the Escherichia coli leucine-responsive regulatory protein (Lrp) was studied by isolating random transcriptional fusions to lacZ, using ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.20.6254-6263.1997
更新日期:1997-10-01 00:00:00
abstract::Gas vesicles, isolated from lysed Halobacterium halobium cells, gave an amino acid analysis which accounted for 78% of the weight, and the balance was mainly salt and water. One percent of tightly bound d-galactose was found, as well as 2% of phosphate that was not released by treatment which promotes beta-elimination...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.3.1058-1067.1973
更新日期:1973-06-01 00:00:00
abstract::Antiserum prepared against protocatechuate 3, 4-dioxygenase from Pseudomonas aeruginosa forms precipitin bands without spurs with isofunctional enzymes from different strains of the fluorescent pseudomonads on immunodiffusion plates. Catalytic activity of the isofunctional enzymes was inhibited by an immunoglobulin fr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.126.1.516-519.1976
更新日期:1976-04-01 00:00:00
abstract::The relative stability of membrane proteins in Escherichia coli was investigated to determine whether these proteins are degraded at heterogeneous rates and, if so, whether the degradative rates are correlated with the sizes or charges of the proteins. Cells growing in a glucose-limited chemostat with a generation tim...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.2.476-483.1981
更新日期:1981-05-01 00:00:00
abstract::We previously showed that dprA is required for efficient processing of linear DNA during cellular transformation in Haemophilus influenzae. In this study the transcriptional regulation of dprA and two downstream genes, dprB and dprC, is examined. We demonstrate by Northern blot analysis that the dprABC genes are trans...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.15.4815-4820.1997
更新日期:1997-08-01 00:00:00
abstract::Two Rhizobium phaseoli mutants, isolated previously by Tn5 mutagenesis, elicited infection threads which ceased development prematurely, usually within root hairs. These infection threads were wide, globular, and otherwise altered in morphology, compared with normal infection threads. Anatomy and division of the root ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.3.1392-1401.1986
更新日期:1986-12-01 00:00:00
abstract::The origin of replication of Bacillus subtilis 168 trp thy dna-1 (temperature-sensitive initiation mutant) was labeled with [3H]thymidine. Analysis of labeled cells by autoradiography revealed that most of the radioactivity was associated with cell pole areas. To label the terminus, cells that had initiated were treat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.3.895-899.1985
更新日期:1985-09-01 00:00:00
abstract::Pyrrolnitrin at 10 mug/ml inhibited the growth of Saccharomyces cerevisiae, Penicillium atrovenetum, and P. oxalicum. The primary site of action of pyrrolnitrin on S. cerevisiae was the terminal electron transport system between succinate or reduced nicotinamide adenine dinucleotide (NADH) and coenzyme Q. At growth in...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.100.1.310-318.1969
更新日期:1969-10-01 00:00:00
abstract::Stevens, Jack G. (University of Washington, Seattle), and Neal B. Groman. Infectious bovine rhinotracheitis virus replication, cytopathology, and plaque formation in the presence and absence of nucleic acid analogues. J. Bacteriol. 87:446-453. 1964.-Cytopathology induced by infectious bovine rhinotracheitis (IBR) viru...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.2.446-453.1964
更新日期:1964-02-01 00:00:00
abstract::Mycobacterium avium-Mycobacterium intracellulare complex (MAC) is the most common isolate of nontuberculous mycobacteria and causes pulmonary and extrapulmonary diseases. MAC species can be grouped into 31 serotypes by the epitopic oligosaccharide structure of the species-specific glycopeptidolipid (GPL) antigen. The ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01850-07
更新日期:2008-05-01 00:00:00
abstract::Formation of photosynthesis complexes in Rhodobacter sphaeroides is regulated in a redox- and light-dependent manner by the AppA/PpsR and PrrB/PrrA systems. While on the one hand, blue light is sensed by the flavin adenine dinucleotide-binding BLUF domain of AppA, on the other, light is absorbed by bacteriochlorophyll...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00262-09
更新日期:2009-07-01 00:00:00
abstract::Acinetobacter baumannii is a severe threat to human health as a frequently multidrug-resistant hospital-acquired pathogen. Part of the danger from this bacterium comes from its genome plasticity and ability to evolve quickly by taking up and recombining external DNA into its own genome in a process called natural comp...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00034-21
更新日期:2021-01-25 00:00:00
abstract::Two types of mutants unable to excrete hemolysin were obtained when E. coli 5K carrying the multicopy hemolytic recombinant plasmid pANN202-312 was mutagenized with Mu d1. One type is altered in the plasmid hly-specific gene, hlyBb, but the other is caused by an insertion of Mu d1 into a chromosomal locus. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.3.1083-1085.1984
更新日期:1984-09-01 00:00:00
abstract::CRISPR-Cas systems are prokaryotic adaptive immune systems that facilitate protection of bacteria and archaea against infection by external mobile genetic elements. The model pathogen Francisella novicida encodes a CRISPR-Cas12a (FnoCas12a) system and a CRISPR-Cas9 (FnoCas9) system, the latter of which has an addition...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00670-19
更新日期:2020-05-27 00:00:00
abstract::Sulfolobus acidocaldarius utilizes glucose and xylose as sole carbon sources, but its ability to metabolize these sugars simultaneously is not known. We report the absence of diauxie during growth of S. acidocaldarius on glucose and xylose as co-carbon sources. The presence of glucose did not repress xylose utilizatio...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01219-10
更新日期:2011-03-01 00:00:00
abstract::Beppu, Michiko (University of Tokyo, Tokyo, Japan), and Kei Arima. Decreased permeability as the mechanism of arsenite resistance in Pseudomonas pseudomallei. J. Bacteriol. 88:151-157. 1964.-The mechanism of arsenite resistance of Pseudomonas pseudomallei strain 54, isolated from soil, was studied by use of radioactiv...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.1.151-157.1964
更新日期:1964-07-01 00:00:00
abstract::The mechanism of methyl-beta-D-thiogalactoside-phosphate (TMG-P) expulsion from Streptococcus pyogenes was studied. The expulsion elicited by glucose was not due to exchange vectorial transphosphorylation between the expelled TMG and the incoming glucose since more beta-galactoside was displaced than glucose taken up,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.1.354-361.1983
更新日期:1983-10-01 00:00:00
abstract::Kimler, Alexander (National Institutes of Health, Bethesda, Md.). Fluorescein amine media for rapid differentiation of staphylococci. J. Bacteriol. 82:106-109. 1961.-By modifying conventional media to include blood, mannitol, and fluorescein amine, staphylococci may be isolated and identified directly. Staphylococcus ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.82.1.106-109.1961
更新日期:1961-07-01 00:00:00
abstract::EspF of enteropathogenic Escherichia coli targets mitochondria and subverts a number of cellular functions. EspF consists of six putative Src homology 3 (SH3) domain binding motifs. In this study we identified sorting nexin 9 (SNX9) as a host cell EspF binding partner protein, which binds EspF via its amino-terminal S...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.8.3110-3115.2006
更新日期:2006-04-01 00:00:00
abstract::Constitutive beta-glucosidases from Saccharomyces fragilis (Y-18) and S. dobzhanskii (Y-19) precipitated at the same concentration of ammonium sulfate. The partially purified enzymes had similar activation energies, molecular weights, affinities for certain natural and synthetic beta-glucosides, and optimal pH values ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.1.135-141.1967
更新日期:1967-01-01 00:00:00
abstract::L. Sibold, M. Henriquet, O. Possot, and J.-P. Aubert (Res. Microbiol. 142:5-12, 1991) cloned and sequenced two nifH-homologous open reading frames (ORFs) from Methanosarcina barkeri 227. Phylogenetic analysis of the deduced amino acid sequences of the nifH ORFs from M. barkeri showed that nifH1 clusters with nifH gene...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.21.6590-6598.1994
更新日期:1994-11-01 00:00:00
abstract::The application of a simple negative staining technique and freeze-etching has revealed detailed information on the species-specific surface structure of spores and aerial hyphae of streptomycetes. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.1.318-322.1970
更新日期:1970-01-01 00:00:00
abstract::From several presumably pure cultures of Thiobacillus ferrooxidans, we isolated a pair of stable phenotypes. One was a strict autotroph utilizing sulfur or ferrous iron as the energy source and unable to utilize glucose; the other phenotype was an acidophilic obligate heterotroph capable of utilizing glucose but not s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.1.448-454.1980
更新日期:1980-07-01 00:00:00
abstract::Bacillus subtilis DegS-DegU belongs to a bacterial two-component system that controls many processes, including the production of exocellular proteases and competence development. It was found that when the glutamine synthetase gene glnA, which is involved in nitrogen regulation, was disrupted, the expression of the r...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00368-08
更新日期:2008-08-01 00:00:00
abstract::The transport of the tricarboxylic acid cycle C(4)-dicarboxylic acids was studied in both the wild-type strain and tricarboxylic acid cycle mutants of Bacillus subtilis. Active transport of malate, fumarate, and succinate was found to be inducible by these dicarboxylic acids or by precursors to them, whereas glucose o...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.1.65-79.1973
更新日期:1973-04-01 00:00:00
abstract::The ultrastructure of seven Thiobacillus species was studied. The structure of their cell envelopes is similar, if not identical, to that found in other gram-negative bacteria. Obvious differences were noted in the middle layer of the cell envelope of the seven cultures. Polyhedral inclusion bodies were apparent in fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.101.2.618-627.1970
更新日期:1970-02-01 00:00:00
abstract:UNLABELLED:Clostridium perfringens type D strains are usually associated with diseases of livestock, and their virulence requires the production of epsilon toxin (ETX). We previously showed (J. Li, S. Sayeed, S. Robertson, J. Chen, and B. A. McClane, PLoS Pathog 7:e1002429, 2011, http://dx.doi.org/10.1371/journal.ppat....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00349-15
更新日期:2015-10-01 00:00:00
abstract::Dworkin, Martin (Indiana University Medical Center, Indianapolis, Ind.). Nutritional requirements for vegetative growth of Myxococcus xanthus. J. Bacteriol. 84:250-257. 1962.-This investigation was part of a program to clarify the environmental regulation of fruiting-body formation in a fruiting myxobacterium. By use ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.2.250-257.1962
更新日期:1962-08-01 00:00:00
abstract::Methionine sulfoxide reductase (Msr) catalyzes the thioredoxin-dependent reduction and repair of methionine sulfoxide (MetO). Although Msr genes are not present in most hyperthermophile genomes, an Msr homolog encoding an MsrA-MsrB fusion protein (MsrAB(Tk)) was present on the genome of the hyperthermophilic archaeon ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00751-06
更新日期:2007-10-01 00:00:00
abstract::The TetA(C) protein, encoded by the tetA(C) gene of plasmid pBR322, is a member of a family of membrane-bound proteins that mediate energy-dependent efflux of tetracycline from the bacterial cell. The tetA(C) gene was mutagenized with hydroxylamine, and missense mutations causing the loss of tetracycline resistance we...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.24.7926-7933.1992
更新日期:1992-12-01 00:00:00