Mutations in Rhizobium phaseoli that lead to arrested development of infection threads.

Abstract:

:Two Rhizobium phaseoli mutants, isolated previously by Tn5 mutagenesis, elicited infection threads which ceased development prematurely, usually within root hairs. These infection threads were wide, globular, and otherwise altered in morphology, compared with normal infection threads. Anatomy and division of the root cortical cells during initial stages of nodule morphogenesis appeared normal. However, later nodule differentiation deviated considerably from normal development, and release of bacteria from infection threads was not observed. In tryptone-yeast extract medium the mutants sedimented during growth in shaken cultures and formed rough colonies on agar. Electrophoresis of washed cultures solubilized in dodecyl sulfate revealed that the major carbohydrate band was absent from the mutants. The behavior of this carbohydrate in phenol-water extraction and gel chromatography, its apparent ketodeoxyoctonate content, and its susceptibility to mild acid hydrolysis suggested that it was a lipopolysaccharide. From the results of genetic crosses or reversion analysis, the defect in synthesizing this carbohydrate material and the defect in infection could be attributed to a single mutation in each mutant.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Noel KD,Vandenbosch KA,Kulpaca B

doi

10.1128/jb.168.3.1392-1401.1986

subject

Has Abstract

pub_date

1986-12-01 00:00:00

pages

1392-401

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

168

pub_type

杂志文章
  • The periplasmic, group III catalase of Vibrio fischeri is required for normal symbiotic competence and is induced both by oxidative stress and by approach to stationary phase.

    abstract::The catalase gene, katA, of the sepiolid squid symbiont Vibrio fischeri has been cloned and sequenced. The predicted amino acid sequence of KatA has a high degree of similarity to the recently defined group III catalases, including those found in Haemophilus influenzae, Bacteroides fragilis, and Proteus mirabilis. Ups...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.8.2087-2092.1998

    authors: Visick KL,Ruby EG

    更新日期:1998-04-01 00:00:00

  • Genophore homologies among compatible nocardiae.

    abstract::Deoxyribonucleic acid (DNA) reassociation analyses were employed to determine the molecular relationships between recombinable nocardiae. Analysis of the compatibility system of Nocardia erythropolis Mat-Ce and Mat-cE mating strains demonstrated the existence of extensive homology under both exacting and nonexacting c...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.2.720-729.1972

    authors: Clark JE,Brownell GH

    更新日期:1972-02-01 00:00:00

  • Involvement of envelope-bound calcium in the transient depolarization of the Escherichia coli cytoplasmic membrane induced by bacteriophage T4 and T5 adsorption.

    abstract::We previously showed that adsorption of bacteriophages T4 and T5 to their respective outer membrane receptors induced a partial depolarization of the cytoplasmic membrane. As these membrane potential changes were independent of phage properties, we proposed that phage adsorption triggered the emission of a signal whic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.157.3.789-794.1984

    authors: Letellier L,Labedan B

    更新日期:1984-03-01 00:00:00

  • Regulation of orotidylic acid pyrophosphorylase in Saccharomyces cerevisiae.

    abstract::Yeast mutants deficient in orotidine-5'-phosphate (OMP) pyrophosphorylase activity have been obtained. The gene encoding this enzyme is unlinked to the four pyrimidine genes previously described. The specific activity of OMP pyrophosphorylase remains constant under all of the physiological conditions used to repress, ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.109.1.196-202.1972

    authors: Jund R,Lacroute F

    更新日期:1972-01-01 00:00:00

  • Natural transformation in Campylobacter species.

    abstract::Growing cells of Campylobacter coli and C. jejuni were naturally transformed by naked DNA without the requirement for any special treatment. Transformation frequencies for homologous chromosomal DNA were approximately 10(-3) transformants per recipient cell in C. coli and 10(-4) in C. jejuni. Maximum competence was fo...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.2.949-955.1990

    authors: Wang Y,Taylor DE

    更新日期:1990-02-01 00:00:00

  • Cloning and sequencing of a cellobiose phosphotransferase system operon from Bacillus stearothermophilus XL-65-6 and functional expression in Escherichia coli.

    abstract::Cellulolytic strains of Bacillus stearothermophilus were isolated from nature and screened for the presence of activities associated with the degradation of plant cell walls. One isolate (strain XL-65-6) which exhibited strong activities with 4-methylumbelliferyl-beta-D-glucopyranoside (MUG) and 4-methylumbelliferyl-b...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.20.6441-6450.1993

    authors: Lai X,Ingram LO

    更新日期:1993-10-01 00:00:00

  • Construction and characterization of an Escherichia coli strain with a uncI mutation.

    abstract::A strain of Escherichia coli with a mutation in the promoter proximal gene ( uncI ) of the unc operon has been constructed by using a new gene replacement method. The mutation is a deletion of a defined sequence of 196 base pairs. It was constructed by homologous integration and segregation of a ColE1-derived recombin...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.3.820-825.1984

    authors: Gay NJ

    更新日期:1984-06-01 00:00:00

  • Chromosomal location of a gene defining nicotinamide deamidase in Escherichia coli.

    abstract::A gene specifying the enzyme nicotinamide deamidase has been mapped, by the interrupted mating technique, at about 33 min on the chromosome of Escherichia coli K-12. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.101.3.1090-1091.1970

    authors: Dickinson ES,Sundaram TK

    更新日期:1970-03-01 00:00:00

  • Nucleoside diphosphate sugar hydrolase gene of Salmonella typhimurium: chromosomal location determined by intergeneric crosses.

    abstract::The gene specifying a membrane-bound nucleoside diphosphate sugar hydrolase of Salmonella typhimurium was mapped near the metA locus by using intergeneric crosses between this bacterium and Escherichia coli. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.137.3.1428-1429.1979

    authors: Minton N,Gunn J,Beacham IR

    更新日期:1979-03-01 00:00:00

  • Transcriptional regulation of the heat shock regulatory gene rpoH in Escherichia coli: involvement of a novel catabolite-sensitive promoter.

    abstract::A catabolite-sensitive promoter was found to be involved in transcription of the heat shock regulatory gene rpoH encoding the sigma 32 protein. Expression of lacZ from the operon fusion, rpoHp-lacZ, was partially inhibited by glucose added to the broth medium. Dissection of the rpoH promoter region allowed us to local...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2710-2715.1990

    authors: Nagai H,Yano R,Erickson JW,Yura T

    更新日期:1990-05-01 00:00:00

  • Transcriptional induction of an Agrobacterium regulatory gene at tandem promoters by plant-released phenolic compounds, phosphate starvation, and acidic growth media.

    abstract::Transcription of the virG gene of Agrobacterium tumefaciens was previously shown to be expressed from two tandem promoters and to be responsive to three stimuli: plant-released phenolic compounds, phosphate starvation, and acidic media. In this report, I describe a set of deletions and other alterations of the 5' end ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.5.2433-2438.1990

    authors: Winans SC

    更新日期:1990-05-01 00:00:00

  • Caulobacter crescentus pili: structure and stage-specific expression.

    abstract::Pili are functionally expressed during the predivisional and swarmer stages of the Caulobacter crescentus differentiation cycle. They appear on the developing swarmer pole and at the same cellular location as flagella and the phiCbK receptor sites. Pili disappear when the swarmer cell differentiates into a stalked cel...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.131.1.340-346.1977

    authors: Lagenaur C,Agabian N

    更新日期:1977-07-01 00:00:00

  • Molecular and expression analysis of the Rhizobium meliloti phosphoenolpyruvate carboxykinase (pckA) gene.

    abstract::The pckA gene of Rhizobium meliloti, encoding phosphoenolpyruvate carboxykinase, was isolated from a genomic cosmid library by complementation of the succinate growth phenotype of a Pck- mutant. The gene region was mapped by subcloning and Tn5 insertion mutagenesis. The DNA sequence for a 2-kb region containing the st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.6.1452-1460.1995

    authors: Osterås M,Driscoll BT,Finan TM

    更新日期:1995-03-01 00:00:00

  • Regulatory roles for IscA and SufA in iron homeostasis and redox stress responses in the cyanobacterium Synechococcus sp. strain PCC 7002.

    abstract::SufA, IscA, and Nfu have been proposed to function as scaffolds in the assembly of Fe/S clusters in bacteria. To investigate the roles of these proteins further, single and double null-mutant strains of Synechococcus sp. strain PCC 7002 were constructed by insertional inactivation of genes homologous to sufA, iscA, an...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.9.3182-3191.2006

    authors: Balasubramanian R,Shen G,Bryant DA,Golbeck JH

    更新日期:2006-05-01 00:00:00

  • Target analysis of mitochondrial genetic units in yeast.

    abstract::Target analyses of the induction of rho(-) mutants indicated that the cytoplasm of an aerobic nonrepressed yeast contains approximately 20 mutable units, whereas repressed cells exhibit approximately 3. Aerobic adaptation of fully repressed cells brings about an increase in these cytoplasmic units which continues afte...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.97.3.1142-1148.1969

    authors: Allen NE,MacQuillan AM

    更新日期:1969-03-01 00:00:00

  • Neutral amino acid transport by membrane vesicles of Streptococcus cremoris is subject to regulation by internal pH.

    abstract::The pH dependence of transport of the neutral amino acids L-serine and L-alanine by membrane vesicles of Streptococcus cremoris have been studied in detail. The rates of four modes of facilitated diffusion (e.g., influx, efflux, exchange, and counterflow) of L-serine and L-alanine increase with increasing H+ concentra...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.6.2748-2754.1987

    authors: Driessen AJ,Kodde J,de Jong S,Konings WN

    更新日期:1987-06-01 00:00:00

  • Isolation and characterization of Escherichia coli phase variants and mutants deficient in type 1 pilus production.

    abstract::Type 1 pili of Escherichia coli are the prototype of the somatic class of pili found on many strains of bacteria. As a first step in the genetic analysis of type 1 piliation, an extensive series of nonpiliated derivatives of E. coli K-12 strain AW405, was characterized to produce attached or free pili when examined in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.130.1.495-505.1977

    authors: Swaney LM,Liu YP,To CM,To CC,Ippen-Ihler K,Brinton CC Jr

    更新日期:1977-04-01 00:00:00

  • A study of iterative type II polyketide synthases, using bacterial genes cloned from soil DNA: a means to access and use genes from uncultured microorganisms.

    abstract::To examine as randomly as possible the role of the beta-ketoacyl and acyl carrier protein (ACP) components of bacterial type II polyketide synthases (PKSs), homologs of the chain-length-factor (CLF) genes were cloned from the environmental community of microorganisms. With PCR primers derived from conserved regions of...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.23.7360-7368.1997

    authors: Seow KT,Meurer G,Gerlitz M,Wendt-Pienkowski E,Hutchinson CR,Davies J

    更新日期:1997-12-01 00:00:00

  • Allostery of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase in Clostridium: another conserved generic characteristic.

    abstract::Enzymological studies were done to characterize the allosteric control of 3-deoxy-d-arabino-heptulosonate 7-phosphate (DAHP) synthetase in three species of Clostridium. Allosteric control was identified as feedback inhibition by phenylalanine and was qualitatively similar for the DAHP synthetases of C. butyricum, C. a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.111.3.641-648.1972

    authors: Jensen RA,Twarog R

    更新日期:1972-09-01 00:00:00

  • Evidence for adenylate nucleotide transport (ATP-ADP translocation) in vesicles of Frankia sp. strain EAN1pec.

    abstract::Atractyloside and carboxyatractyloside partially inhibited nitrogenase activity (acetylene reduction) by isolated vesicles of Frankia strain EAN1pec. Extracts of disrupted vesicles showed nitrogenase activity that was not affected by the inhibitors. The vesicles accumulated ATP by an atractyloside-sensitive mechanism....

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.7.3053-3057.1988

    authors: Tisa LS,Ensign JC

    更新日期:1988-07-01 00:00:00

  • Genetic analysis of the chitinase system of Serratia marcescens 2170.

    abstract::To carry out a genetic analysis of the degradation and utilization of chitin by Serratia marcescens 2170, various Tn5 insertion mutants with characteristic defects in chitinase production were isolated and partially characterized. Prior to the isolation of the mutants, proteins secreted into culture medium in the pres...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.22.7111-7117.1997

    authors: Watanabe T,Kimura K,Sumiya T,Nikaidou N,Suzuki K,Suzuki M,Taiyoji M,Ferrer S,Regue M

    更新日期:1997-11-01 00:00:00

  • Isolation and characterization of yeast monomorphic mutants of Candida albicans.

    abstract::A method was devised for the isolation of yeast monomorphic (LEV) mutants of Candida albicans. By this procedure, about 20 stable yeast-like mutants were isolated after mutagenesis with ethyl methane sulfonate. The growth rate of the mutants in different carbon sources, both fermentable and not, was indistinguishable ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.8.2318-2325.1994

    authors: Elorza MV,Sentandreu R,Ruiz-Herrera J

    更新日期:1994-04-01 00:00:00

  • Mutagenesis of the Tra1 core region of RK2 by using Tn5: identification of plasmid-specific transfer genes.

    abstract::The conjugation system of the IncP alpha plasmid RK2/RP4 is encoded by transfer regions designated Tra1, Tra2, and Tra3. The Tra1 core region, cloned on plasmid pDG4 delta 22, consists of the origin of transfer (oriT) and 2.6 kilobases of flanking DNA providing IncP alpha plasmid-specific functions that allow pDG4 del...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.7.4100-4103.1989

    authors: Guiney DG,Deiss C,Simnad V,Yee L,Pansegrau W,Lanka E

    更新日期:1989-07-01 00:00:00

  • EFFECT OF HIGH FAT DIETS ON INTESTINAL MICROFLORA AND SERUM CHOLESTEROL IN RATS.

    abstract::Graber, C. D. (Baylor University College of Medicine, Houston, Tex.), R. M. O'Neal, and E. R. Rabin. Effect of high fat diets on intestinal microflora and serum cholesterol in rats. J. Bacteriol. 89:47-51. 1965.-Differential bacterial counts of feces and total plasma cholesterol determinations were performed on 60 Wis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.89.1.47-51.1965

    authors: GRABER CD,O'NEAL RM,RABIN ER

    更新日期:1965-01-01 00:00:00

  • Stimulation of adenosine 5'-triphosphate-dependent in vitro deoxyribonucleic acid replication by factors from the periplasmic space of Escherichia coli.

    abstract::In vitro deoxyribonucleic acid (DNA) synthesis systems based on an earlier system using pencillin have been developed which use osmotic lysis of lysozyme-formed spheroplasts of Escherichia coli cells embedded in an agarose matrix. An adenosine 5'-triphosphate (ATP)-dependent semiconservative mode, or replicative mode,...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.122.1.159-170.1975

    authors: Smith DW,Boerner P

    更新日期:1975-04-01 00:00:00

  • Multiple signals direct the assembly and function of a type 1 secretion system.

    abstract::Type 1 secretion systems (T1SS) are present in a wide range of Gram-negative bacteria and are involved in the secretion of diverse substrates such as proteases, lipases, and hemophores. T1SS consist of three proteins: an inner membrane ABC (ATP binding cassette) protein, a periplasmic adaptor, and an outer membrane ch...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00178-10

    authors: Masi M,Wandersman C

    更新日期:2010-08-01 00:00:00

  • trp RNA-binding attenuation protein (TRAP)-trp leader RNA interactions mediate translational as well as transcriptional regulation of the Bacillus subtilis trp operon.

    abstract::Expression of the Bacillus subtilis trpEDCFBA operon has been shown to be regulated by transcription attenuation in response to the availability of L-tryptophan. Regulation is mediated by the tryptophan-activated trp RNA-binding attenuation protein, TRAP, the product of mtrB. Formation of mutually exclusive RNA anti-t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.22.6362-6370.1995

    authors: Merino E,Babitzke P,Yanofsky C

    更新日期:1995-11-01 00:00:00

  • Genome-based analysis and gene dosage studies provide new insight into 3-hydroxy-4-methylvalerate biosynthesis in Ralstonia eutropha.

    abstract::Recombinant Ralstonia eutropha strain PHB(-)4 expressing the broad-substrate-specificity polyhydroxyalkanoate (PHA) synthase 1 from Pseudomonas sp. strain 61-3 (PhaC1Ps) synthesizes a PHA copolymer containing the branched side-chain unit 3-hydroxy-4-methylvalerate (3H4MV), which has a carbon backbone identical to that...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.02474-14

    authors: Saika A,Ushimaru K,Mizuno S,Tsuge T

    更新日期:2015-04-01 00:00:00

  • Gene cluster of Pseudomonas syringae pv. "phaseolicola" controls pathogenicity of bean plants and hypersensitivity of nonhost plants.

    abstract::Loss of the ability of Pseudomonas syringae pv. "phaseolicola" NPS3121 to elicit a hypersensitive response on tobacco and other nonhost plants was associated with loss of pathogenicity on the susceptible host bean. Eight independent, prototrophic transposon Tn5 insertion mutants which had lost the ability to elicit a ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.168.2.512-522.1986

    authors: Lindgren PB,Peet RC,Panopoulos NJ

    更新日期:1986-11-01 00:00:00

  • Mycobacterium tuberculosis Latin American-Mediterranean family and its sublineages in the light of robust evolutionary markers.

    abstract::Mycobacterium tuberculosis has a clonal population structure, and the Latin American-Mediterranean (LAM) family is one of the largest and most widespread within this species, showing evidence for remarkable pathobiology and a confusing phylogeny. Here, we applied robust phylogenetic markers to study the evolution of t...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01485-13

    authors: Mokrousov I,Vyazovaya A,Narvskaya O

    更新日期:2014-05-01 00:00:00