Genetic analysis of mecillinam-resistant mutants of Caulobacter crescentus deficient in stalk biosynthesis.

Abstract:

:Stalk synthesis in Caulobacter crescentus is a developmentally controlled and spatially restricted event that requires the synthesis of peptidoglycan at the stalk-cell body junction. We show that the beta-lactam antibiotic mecillinam prevents stalk synthesis by inhibiting stalk elongation. In addition, mecillinam causes an increase in the diameter of the stalk at the stalk-cell body junction. We describe two mutations that confer resistance to mecillinam and that prevent stalk elongation. These mutations are probably allelic, and they map to a locus previously not associated with stalk synthesis.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Seitz LC,Brun YV

doi

10.1128/JB.180.19.5235-5239.1998

subject

Has Abstract

pub_date

1998-10-01 00:00:00

pages

5235-9

issue

19

eissn

0021-9193

issn

1098-5530

journal_volume

180

pub_type

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