Effect of growth temperature on Crl-dependent regulation of sigmaS activity in Salmonella enterica serovar Typhimurium.

Abstract:

:The small regulatory protein Crl favors association of the stationary-phase sigma factor sigma(S) (RpoS) with the core enzyme polymerase and thereby increases sigma(S) activity. Crl has a major physiological impact at low levels of sigma(S). Here, we report that the Crl effects on sigma(S)-dependent gene expression, the H(2)O(2) resistance of Salmonella enterica serovar Typhimurium, and the resistance of this organism to acidic pH are greater at 28 degrees C than at 37 degrees C. Immunoblot experiments revealed a negative correlation between sigma(S) and Crl levels; the production of Crl was slightly greater at 28 degrees C than at 37 degrees C, whereas the sigma(S) levels were about twofold lower at 28 degrees C than at 37 degrees C. At both temperatures, Crl was present in excess of sigma(S), and increasing the Crl level further did not increase the H(2)O(2) resistance level of Salmonella and the expression of the sigma(S)-dependent gene katE encoding the stationary-phase catalase. In contrast, increasing the sigma(S) level rendered Salmonella more resistant to H(2)O(2) at 28 degrees C, increased the expression of katE, and reduced the magnitude of Crl activation. In addition, the effect of Crl on katE transcription in vitro was not dependent on temperature. These results suggest that the effect of temperature on Crl-dependent regulation of the katE gene and H(2)O(2) resistance are mediated mainly via an effect on sigma(S) levels. In addition, our results revealed that sigma(S) exerts a negative effect on the production of Crl in stationary phase when the cells contain high levels of sigma(S).

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Robbe-Saule V,Carreira I,Kolb A,Norel F

doi

10.1128/JB.00154-08

subject

Has Abstract

pub_date

2008-07-01 00:00:00

pages

4453-9

issue

13

eissn

0021-9193

issn

1098-5530

pii

JB.00154-08

journal_volume

190

pub_type

杂志文章
  • Functional Characterization of a Novel Member of the Amidohydrolase 2 Protein Family, 2-Hydroxy-1-Naphthoic Acid Nonoxidative Decarboxylase from Burkholderia sp. Strain BC1.

    abstract:UNLABELLED:The gene encoding a nonoxidative decarboxylase capable of catalyzing the transformation of 2-hydroxy-1-naphthoic acid (2H1NA) to 2-naphthol was identified, recombinantly expressed, and purified to homogeneity. The putative gene sequence of the decarboxylase (hndA) encodes a 316-amino-acid protein (HndA) with...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00250-16

    authors: Pal Chowdhury P,Basu S,Dutta A,Dutta TK

    更新日期:2016-05-27 00:00:00

  • Regulation of cytotoxicity by quorum-sensing signaling in Vibrio vulnificus is mediated by SmcR, a repressor of hlyU.

    abstract::Cytotoxicity is an important virulence determinant in the pathogenesis of Vibrio vulnificus, and two cytotoxins, RTX (encoded by rtxA1) and cytolysin/hemolysin (encoded by vvhA), have been identified in this organism. We showed that the quorum-sensing regulator LuxO controlled the cytotoxicity of this organism: a Δlux...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01259-10

    authors: Shao CP,Lo HR,Lin JH,Hor LI

    更新日期:2011-05-01 00:00:00

  • Mutation of waaC, encoding heptosyltransferase I in Campylobacter jejuni 81-176, affects the structure of both lipooligosaccharide and capsular carbohydrate.

    abstract::Campylobacter jejuni 81-176 lipooligosaccharide (LOS) is composed of two covalently linked domains: lipid A, a hydrophobic anchor, and a nonrepeating core oligosaccharide, consisting of an inner and outer core region. We report the isolation and characterization of the deepest rough C. jejuni 81-176 mutant by insertio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.9.3273-3279.2006

    authors: Kanipes MI,Papp-Szabo E,Guerry P,Monteiro MA

    更新日期:2006-05-01 00:00:00

  • Stringent and relaxed recognition of oriT by related systems for plasmid mobilization: implications for horizontal gene transfer.

    abstract::The plasmids R1162 and pSC101 have origins of conjugative transfer (oriTs) and corresponding relaxases that are closely related. The oriTs are made up of a highly conserved core, where DNA is cleaved by the relaxase prior to transfer, and an inverted repeat that differs in size and sequence. We show that in each case ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.188.2.499-506.2006

    authors: Jandle S,Meyer R

    更新日期:2006-01-01 00:00:00

  • Effective symbiosis between Rhizobium etli and Phaseolus vulgaris requires the alarmone ppGpp.

    abstract::The symbiotic interaction between Rhizobium etli and Phaseolus vulgaris, the common bean plant, ultimately results in the formation of nitrogen-fixing nodules. Many aspects of the intermediate and late stages of this interaction are still poorly understood. The R. etli relA gene was identified through a genome-wide sc...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5460-5469.2005

    authors: Moris M,Braeken K,Schoeters E,Verreth C,Beullens S,Vanderleyden J,Michiels J

    更新日期:2005-08-01 00:00:00

  • Amino acid substitution in the lactose carrier protein with the use of amber suppressors.

    abstract::Five lacY mutants with amber stop codons at known positions were each placed into 12 different suppressor strains. The 60 amino acid substitutions obtained in this manner were tested for growth on lactose-minimal medium plates and for transport of lactose, melibiose, and thiomethylgalactoside. Most of the amino acid s...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.174.16.5436-5441.1992

    authors: Huang AM,Lee JI,King SC,Wilson TH

    更新日期:1992-08-01 00:00:00

  • Conjugative transfer of the integrative conjugative elements ICESt1 and ICESt3 from Streptococcus thermophilus.

    abstract::Integrative and conjugative elements (ICEs), also called conjugative transposons, are genomic islands that excise, self-transfer by conjugation, and integrate in the genome of the recipient bacterium. The current investigation shows the intraspecies conjugative transfer of the first described ICEs in Streptococcus the...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01412-08

    authors: Bellanger X,Roberts AP,Morel C,Choulet F,Pavlovic G,Mullany P,Decaris B,Guédon G

    更新日期:2009-04-01 00:00:00

  • Maltose uptake by the novel ABC transport system MusEFGK2I causes increased expression of ptsG in Corynebacterium glutamicum.

    abstract::The Gram-positive Corynebacterium glutamicum efficiently metabolizes maltose by a pathway involving maltodextrin and glucose formation by 4-α-glucanotransferase, glucose phosphorylation by glucose kinases, and maltodextrin degradation via maltodextrin phosphorylase and α-phosphoglucomutase. However, maltose uptake in ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01629-12

    authors: Henrich A,Kuhlmann N,Eck AW,Krämer R,Seibold GM

    更新日期:2013-06-01 00:00:00

  • Genome sequence of the naturally plasmid-free Lactobacillus plantarum strain NC8 (CCUG 61730).

    abstract::Lactobacillus plantarum is a highly versatile lactic acid bacterium found in various ecological niches, such as fermented vegetable, meat, and dairy products and the gastrointestinal tract. We sequenced the genome of L. plantarum NC8, a naturally plasmid-free strain, which has been used as a model strain in many labor...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00141-12

    authors: Axelsson L,Rud I,Naterstad K,Blom H,Renckens B,Boekhorst J,Kleerebezem M,van Hijum S,Siezen RJ

    更新日期:2012-05-01 00:00:00

  • Facile and gentle method for quantitative lysis of Escherichia coli and Salmonella typhimurium.

    abstract::Garrett et al. (Mol. Gen. Genet. 182:326-331, 1981) constructed strains of Escherichia coli harboring derivatives of plasmid pBR322 that carry the lysis genes (S, R, and Rz) of phage lambda. The plasmid construction placed the genes under control of the lactose operon operator-promotor (and thus of lac repressor). Ind...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.158.1.354-356.1984

    authors: Crabtree S,Cronan JE Jr

    更新日期:1984-04-01 00:00:00

  • Bacillus subtilis gene involved in cell division, sporulation, and exoenzyme secretion.

    abstract::Bacillus subtilis strains carrying div-341 or sacU mutations, or both, have been characterized to reveal the roles of both genes in the initiation of sporulation, as well as in cell division and exoenzyme secretion. Both mutations were closely linked by transformation and caused the pleiotropic effects on sporulation ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.163.2.648-653.1985

    authors: Sadaie Y,Kada T

    更新日期:1985-08-01 00:00:00

  • Galactose transport in Saccharomyces cerevisiae. I. Nonmetabolized sugars as substrates and inducers of the galactose transport system.

    abstract::The inducible galactose transport system in bakers' yeast carries out the facilitated diffusion of the nonmetabolized galactose analogues d-fucose and l-arabinose. This capacity depends on the activity of the Ga 2 gene. In some strains, d-fucose and l-arabinose are also gratuitous inducers. Mutants in which the induci...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.95.5.1727-1731.1968

    authors: Cirillo VP

    更新日期:1968-05-01 00:00:00

  • Correlation between acrasins and spore germination inhibitors in cellular slime molds.

    abstract::Discadenine,3-(3-amino-3-carboxypropyl)-N6-delta 2-isopentenyladenine, which inhibits spore germination, was previously found in Dictyostelium discoideum. Studies on the distribution of discadenine in different species of cellular slime molds by high-pressure liquid chromatography showed that discadenine is present in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.143.2.715-719.1980

    authors: Taya Y,Yamada T,Nishimura S

    更新日期:1980-08-01 00:00:00

  • Interaction of related Tn916-like transposons: analysis of excision events promoted by Tn916 and Tn5386 integrases.

    abstract::In recent work, we described the excision of a large genomic region from Enterococcus faecium D344R in which the sequence from "joint" regions suggested that excision resulted from the interaction of conjugative transposon Tn916 and the related mobile element Tn5386. In the present study, we examined the ability of in...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00859-06

    authors: Rice LB,Carias LL,Hutton-Thomas R,Rudin S

    更新日期:2007-05-01 00:00:00

  • Ultraviolet light sensitivity of Escherichia coli K-12 strains carrying ruv mutations in combination with uvrA or lon mutant alleles.

    abstract::Strains of Escherichia coli K12 have been prepared that carry the ruv mutation in combination with lon or uvrA mutant alleles. The ruv minus uvrA minus double mutant is more sensitive to ultraviolet light than the urvA minus single mutant, whereas the strain with ruv and ion mutations shows the same sensitivity to ult...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.121.2.735-736.1975

    authors: Iyehara H,Otsuji N

    更新日期:1975-02-01 00:00:00

  • Regulation of expression and nucleotide sequence of the Anabaena variabilis recA gene.

    abstract::The expression of the cyanobacterial recA gene, isolated from Anabaena variabilis, has been examined at the levels of transcript and protein abundance. Exposure of the cyanobacterium to a variety of DNA-damaging agents, including mitomycin C, methyl methanesulfonate, and UV irradiation, results in a rapid increase in ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.171.10.5713-5719.1989

    authors: Owttrim GW,Coleman JR

    更新日期:1989-10-01 00:00:00

  • Promoter recognition and activation by the global response regulator CbrB in Pseudomonas aeruginosa.

    abstract::In Pseudomonas aeruginosa, the CbrA/CbrB two-component system is instrumental in the maintenance of the carbon-nitrogen balance and for growth on carbon sources that are energetically less favorable than the preferred dicarboxylate substrates. The CbrA/CbrB system drives the expression of the small RNA CrcZ, which ant...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00164-11

    authors: Abdou L,Chou HT,Haas D,Lu CD

    更新日期:2011-06-01 00:00:00

  • Characterization of CMP-N-acetylneuraminic acid synthetase of group B streptococci.

    abstract::The capsular polysaccharide is a critical virulence factor for group B streptococci associated with human infections, yet little is known about capsule biosynthesis. We detected CMP-Neu5Ac synthetase, the enzyme which activates N-acetylneuraminic acid (Neu5Ac, or sialic acid) for transfer to the nascent capsular polys...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.23.7372-7374.1994

    authors: Haft RF,Wessels MR

    更新日期:1994-12-01 00:00:00

  • Isolation of a high-affinity functional protein complex between OmcA and MtrC: Two outer membrane decaheme c-type cytochromes of Shewanella oneidensis MR-1.

    abstract::Shewanella oneidensis MR-1 is a facultatively anaerobic bacterium capable of using soluble and insoluble forms of manganese [Mn(III/IV)] and iron [Fe(III)] as terminal electron acceptors during anaerobic respiration. To assess the structural association of two outer membrane-associated c-type decaheme cytochromes (i.e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01966-05

    authors: Shi L,Chen B,Wang Z,Elias DA,Mayer MU,Gorby YA,Ni S,Lower BH,Kennedy DW,Wunschel DS,Mottaz HM,Marshall MJ,Hill EA,Beliaev AS,Zachara JM,Fredrickson JK,Squier TC

    更新日期:2006-07-01 00:00:00

  • Genetic and physical analyses of Methylobacterium organophilum XX genes encoding methanol oxidation.

    abstract::When allyl alcohol was used as a suicide substrate, spontaneous mutants and UV light- and nitrous acid-generated mutants of Methylobacterium organophilum XX were selected which grew on methylamine but not on methanol. There was no detectable methanol dehydrogenase (MDH) activity in crude extracts of these mutants, yet...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.170.1.141-148.1988

    authors: Machlin SM,Tam PE,Bastien CA,Hanson RS

    更新日期:1988-01-01 00:00:00

  • Method for fingerprinting yeast cell wall mannans.

    abstract::Controlled acetolysis of yeast mannans yields mixtures of oligosaccharides with (1-->2) and (1-->3) linkages between the mannose units, whereas the less stable (1-->6) linkages of the polysaccharide backbone are cleaved. The "fingerprints," obtained by gel filtration of the oligosaccharide mixtures, can be used to dis...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.100.3.1175-1181.1969

    authors: Kocourek J,Ballou CE

    更新日期:1969-12-01 00:00:00

  • Suppressor analysis of mutations in the loop 2-3 motif of lactose permease: evidence that glycine-64 is an important residue for conformational changes.

    abstract::A superfamily of transport proteins, which includes the lactose permease of Escherichia coli, contains a highly conserved motif, G-X-X-X-D/E-R/K-X-G-R/K-R/K, in the loops that connect transmembrane segments 2 and 3 and transmembrane segments 8 and 9. Previous analysis of this motif in the lactose permease (A. E. Jesse...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.8.2616-2622.1997

    authors: Jessen-Marshall AE,Parker NJ,Brooker RJ

    更新日期:1997-04-01 00:00:00

  • Expression of glnA in the cyanobacterium Synechococcus sp. strain PCC 7942 is initiated from a single nif-like promoter under various nitrogen conditions.

    abstract::The glnA mRNA, encoding glutamine synthetase, is differentially accumulated in the cyanobacterium Synechococcus sp. strain PCC 7942 in media containing different nitrogen sources. With the different nitrogen compounds, transcription of glnA initiated at a single site located -146 nucleotides upstream of the translatio...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.23.7727-7731.1993

    authors: Cohen-Kupiec R,Gurevitz M,Zilberstein A

    更新日期:1993-12-01 00:00:00

  • Mutations that affect control of the methylesterase activity of CheB, a component of the chemotaxis adaptation system in Escherichia coli.

    abstract::Sensory adaptation by the chemotaxis system of Escherichia coli requires adjustments of the extent of methyl esterification of the chemotaxis receptor proteins. One mechanism utilized by E. coli to make such adjustments is to control the activity of CheB, the enzyme responsible for removing receptor methyl ester group...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.6.3388-3399.1990

    authors: Stewart RC,Roth AF,Dahlquist FW

    更新日期:1990-06-01 00:00:00

  • Aerobactin utilization by Neisseria gonorrhoeae and cloning of a genomic DNA fragment that complements Escherichia coli fhuB mutations.

    abstract::Aerobactin, a dihydroxamate siderophore produced by many strains of enteric bacteria, stimulated the growth of Neisseria gonorrhoeae FA19 and F62 in iron-limiting medium. However, gonococci did not produce detectable amounts of aerobactin in the Escherichia coli LG1522 aerobactin bioassay. We probed gonococcal genomic...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.169.8.3414-3421.1987

    authors: West SE,Sparling PF

    更新日期:1987-08-01 00:00:00

  • INTERACTION OF AN INTERFERON WITH L CELLS.

    abstract::Lockart, Royce Z., Jr. (The University of Texas, Austin) and Barbara Horn. Interaction of an interferon with L cells. J. Bacteriol. 85:996-1002. 1963.-Data were presented on the effect of time of exposure and concentration of an interferon in provoking viral inhibition in L cells. Populations of L cells which made red...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.85.5.996-1002.1963

    authors: LOCKART RZ Jr,HORN B

    更新日期:1963-05-01 00:00:00

  • Occurrence of alpha-tocopherolquinone and alpha-tocopherolquinol in microorganisms.

    abstract::Both alpha-tocopherolquinol and alpha-tocopherolquinone were found in 56 of 93 strains of microorganisms examined. Organisms that contained these compounds included the single example of a eucaryotic alga, a Euglena, and a cyanobacterium (blue-green alga), 22 of 32 genera of bacteria, and 9 genera of yeasts. In the ba...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.151.3.1397-1402.1982

    authors: Hughes PE,Tove SB

    更新日期:1982-09-01 00:00:00

  • Identification of essential glutamates in the acetate kinase from Methanosarcina thermophila.

    abstract::Acetate kinase catalyzes the reversible phosphorylation of acetate (CH3COO- + ATP<-->CH3CO2PO3(2-) + ADP). A mechanism which involves a covalent phosphoryl-enzyme intermediate has been proposed, and chemical modification studies of the enzyme from Escherichia coli indicate an unspecified glutamate residue is phosphory...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.5.1129-1134.1998

    authors: Singh-Wissmann K,Ingram-Smith C,Miles RD,Ferry JG

    更新日期:1998-03-01 00:00:00

  • Relationship between deficiency of phosphoglucose isomerase in Coprinus macrorhizus and fruiting body formation.

    abstract::A mutant (pgi) of Coprinus macrorhizus deficient in phosphoglucose isomerase did not grow on fructose and grew poorly on glucose. The pgi mutation inhibited the formation of monokaryotic and dikaryotic fruiting bodies. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.144.1.432-434.1980

    authors: Nyunoya H,Ishikawa T

    更新日期:1980-10-01 00:00:00

  • Vibrio cholerae enterotoxin genes: nucleotide sequence analysis of DNA encoding ADP-ribosyltransferase.

    abstract::The nucleotide sequence of the DNA encoding the ADP-ribosyltransferase (A1) fragment of cholera enterotoxin was determined. A putative precursor of the A1 peptide contains an 18-amino acid leader peptide, and the mature A1 peptide contains 194 amino acids. The primary structure of the A1 fragment from cholera enteroto...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.159.3.1086-1089.1984

    authors: Lockman HA,Galen JE,Kaper JB

    更新日期:1984-09-01 00:00:00