Abstract:
:The riboflavin analog roseoflavin is an antibiotic produced by Streptomyces davawensis. Riboflavin transporters are responsible for roseoflavin uptake by target cells. Roseoflavin is converted to the flavin mononucleotide (FMN) analog roseoflavin mononucleotide (RoFMN) by flavokinase and to the flavin adenine dinucleotide (FAD) analog roseoflavin adenine dinucleotide (RoFAD) by FAD synthetase. In order to study the effect of RoFMN and RoFAD in the cytoplasm of target cells, Escherichia coli was used as a model. E. coli is predicted to contain 38 different FMN- or FAD-dependent proteins (flavoproteins). These proteins were overproduced in recombinant E. coli strains grown in the presence of sublethal amounts of roseoflavin. The flavoproteins were purified and analyzed with regard to their cofactor contents. It was found that 37 out of 38 flavoproteins contained either RoFMN or RoFAD. These cofactors have different physicochemical properties than FMN and FAD and were reported to reduce or completely abolish flavoprotein function.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Langer S,Hashimoto M,Hobl B,Mathes T,Mack Mdoi
10.1128/JB.00646-13subject
Has Abstractpub_date
2013-09-01 00:00:00pages
4037-45issue
18eissn
0021-9193issn
1098-5530pii
JB.00646-13journal_volume
195pub_type
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更新日期:1990-03-01 00:00:00
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更新日期:2015-08-31 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.96.6.1982-1990.1968
更新日期:1968-12-01 00:00:00
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更新日期:1977-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.3.912-918.1981
更新日期:1981-12-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1987-08-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1982-04-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1984-11-01 00:00:00
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更新日期:1982-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1986-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1990-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1997-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2013-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1999-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.168.1.115-122.1986
更新日期:1986-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.114.1.267-272.1973
更新日期:1973-04-01 00:00:00