Effect of phenylbutazone on phagocytosis and intracellular killing by guinea pig polymorphonuclear leukocytes.

Abstract:

:The anti-inflammatory drug phenylbutazone has been found to inhibit both engulfment and intracellular killing of E. coli by guinea pig peritoneal polymorphonuclear (PMN) leukocytes. The bactericidal activity of leukocytic homogenates was also inhibited by the drug. Addition of the drug at various time intervals to a phagocytic reacting system caused an almost immediate cessation of bactericidal activity. Metabolic studies showed that the drug sharply curtailed glucose-l-(14)C and (14)C-formate oxidation of both resting and phagocytizing PMN leukocytes. These data indicated an effect upon the hexose monophosphate shunt and H(2)O(2) formation. Further investigation showed that the sites of inhibition were on glucose-6-phosphate and 6-phosphogluconate dehydrogenase. These inhibitions resulted in decreased H(2)O(2) production. It is suggested that H(2)O(2) activates lysosomes and subsequently complexes with the lysosomal enzyme, myeloperoxidase. This complex is a potent bactericidal agent in the phagocyte.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Strauss RR,Paul BB,Sbarra AJ

doi

10.1128/JB.96.6.1982-1990.1968

subject

Has Abstract

pub_date

1968-12-01 00:00:00

pages

1982-90

issue

6

eissn

0021-9193

issn

1098-5530

journal_volume

96

pub_type

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