Microbial degradation of beta-chlorinated four-carbon aliphatic acids.

Abstract:

:Alcaligenes sp. strain CC1 is able to grow on several alpha-chlorinated aliphatic acids (2-chlorobutyrate, 2-chloropropionate, and chloroacetate), as well as on the beta-chlorinated four-carbon aliphatic acids trans-3-chlorocrotonate, cis-3-chlorocrotonate, and 3-chlorobutyrate as sole carbon and energy sources. Dehalogenation of alpha-chlorinated acids could be measured by using resting cells grown on all the different carbon sources, whereas dehalogenation of beta-chlorinated four-carbon acids could be detected only by using resting cells grown on four-carbon compounds. A constitutive 2-haloacid dehalogenase, which did not show any activity with beta-chlorinated four-carbon acids, was detected in cell extracts. Cell extracts of crotonate-grown cells additionally contained a beta-haloacid dechlorination activity, which acted on trans-3-chlorocrotonate, cis-3-chlorocrotonate, and 3-chlorobutyrate and was strictly dependent on coenzyme A, ATP, and Mg2+. Dechlorination of beta-chlorinated four-carbon acids takes place after activation of the acids to their coenzyme A derivatives and seems to be independent of the constitutive 2-haloacid dehalogenase.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Kohler-Staub D,Kohler HP

doi

10.1128/jb.171.3.1428-1434.1989

subject

Has Abstract

pub_date

1989-03-01 00:00:00

pages

1428-34

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

171

pub_type

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