Mutagenesis of the Tra1 core region of RK2 by using Tn5: identification of plasmid-specific transfer genes.

abstract：:The physiological changes in an F1-ATPase-defective mutant of Escherichia coli W1485 growing in a glucose-limited chemostat included a decreased growth yield (60%) and increased specific rates of both glucose consumption (168%) and respiration (171%). Flux analysis revealed that the mutant showed approximately twice a...

journal_title：Journal of bacteriology

authors： Noda S,Takezawa Y,Mizutani T,Asakura T,Nishiumi E,Onoe K,Wada M,Tomita F,Matsushita K,Yokota A

更新日期：2006-10-01 00:00:00

abstract：:Vibrio cholerae is the etiologic agent of cholera in humans. Intestinal colonization occurs in a stepwise fashion, initiating with attachment to the small intestinal epithelium. This attachment is followed by expression of the toxin-coregulated pilus, microcolony formation, and cholera toxin (CT) production. We have r...

journal_title：Journal of bacteriology

authors： Jude BA,Martinez RM,Skorupski K,Taylor RK

更新日期：2009-11-01 00:00:00

abstract：:Isolated cell envelopes of a marine bacterium, M.B.3, have been prepared which possess a nonspecific, cation-activated nucleotidase. The cell envelope comprises approximately 35% (dry weight) of the whole cell and contains protein, 60.2%; lipids, 20.7%; hexose, 3.4%; and ribonucleic acid, 4.6%. No deoxyribonucleic aci...

journal_title：Journal of bacteriology

authors： Thompson J,Green ML,Happold FC

更新日期：1969-09-01 00:00:00

abstract：:Two mutations in the ninR region of bacteriophage lambda that bypass a requirement for antitermination have been studied. One mutation, byp, has been cloned and mapped by marker rescue to a 417-base-pair segment in the ninR region of the genome. Analysis of the byp mutation by using promoter detection vectors, DNA seq...

journal_title：Journal of bacteriology

authors： Costantino N,Zuber M,Court D

更新日期：1990-08-01 00:00:00

abstract：:Agrobacterium radiobacter NCIB 11883 was grown in lactose-limited continuous culture at a dilution rate of 0.045/h. Washed cells transported [14C]lactose and [methyl-14C]beta-D-thiogalactoside, a nonmetabolisable analog of lactose, at similar rates and with similar affinities (Km for transport, less than 1 microM). Tr...

journal_title：Journal of bacteriology

authors： Greenwood JA,Cornish A,Jones CW

更新日期：1990-04-01 00:00:00

abstract：:The morphology, on MacConkey's agar, of colonies of the bacteria commonly found in enteric specimens is sufficiently characteristic and distinctive, when observed with transmitted oblique illumination and a low-power stereoscopic microscope, to enable identification at the generic (and occasionally the species) level ...

journal_title：Journal of bacteriology

authors： Finkelstein RA,Punyashthiti K

更新日期：1967-06-01 00:00:00

abstract：:Lactococcus garvieae is the etiological agent of lactococcosis, one of the most important disease threats to the sustainability of the rainbow trout farming industry. Here, we present the draft genome sequence of Lactococcus garvieae strain 8831, isolated from diseased rainbow trout, which is composed of 2,087,276 bp ...

journal_title：Journal of bacteriology

authors： Aguado-Urda M,López-Campos GH,Gibello A,Cutuli MT,López-Alonso V,Fernández-Garayzábal JF,Blanco MM

更新日期：2011-08-01 00:00:00

abstract：:Agrobacterium tumefaciens VirD2 polypeptide, in the presence of VirD1, catalyzes a site- and strand-specific nicking reaction at the T-DNA border sequences. VirD2 is found tightly attached to the 5' end of the nicked DNA. The protein-DNA complex is presumably formed via a tyrosine residue of VirD2 (F. Durrenberger, A....

journal_title：Journal of bacteriology

authors： Vogel AM,Das A

更新日期：1992-01-01 00:00:00

abstract：:Here we describe that the Helicobacter pylori sensor kinase produced by HP1364 and the response regulator produced by HP1365 and designated CrdS and CrdR, respectively, are both required for transcriptional induction of the H. pylori copper resistance determinant CrdA by copper ions. CrdRS-deficient mutants lacked cop...

journal_title：Journal of bacteriology

authors： Waidner B,Melchers K,Stähler FN,Kist M,Bereswill S

更新日期：2005-07-01 00:00:00

abstract：:The lipid intermediate in peptidoglycan biosynthesis was isolated from Escherichia coli strain W and characterized as C(55)-isoprenyl-pyrophosphoryl N-acetylmuramyl(-pentapeptide)-N-acetyl-glucosamine. ...

journal_title：Journal of bacteriology

authors： Umbreit JN,Strominger JL

更新日期：1972-12-01 00:00:00

abstract：:SigB is an alternative sigma factor that controls a large regulon in Staphylococcus aureus. Activation of SigB requires RsbU, a protein phosphatase 2C (PP2C)-type phosphatase. In a closely related organism, Bacillus subtilis, RsbU activity is stimulated upon interaction with RsbT, a kinase, which following an activati...

journal_title：Journal of bacteriology

authors： Pané-Farré J,Jonas B,Hardwick SW,Gronau K,Lewis RJ,Hecker M,Engelmann S

更新日期：2009-04-01 00:00:00

abstract：:The general stress response in Alphaproteobacteria was recently described to depend on the alternative sigma factor σ(EcfG), whose activity is regulated by its anti-sigma factor NepR. The response regulator PhyR, in turn, regulates NepR activity in a partner-switching mechanism according to which phosphorylation of Ph...

journal_title：Journal of bacteriology

authors： Kaczmarczyk A,Campagne S,Danza F,Metzger LC,Vorholt JA,Francez-Charlot A

更新日期：2011-12-01 00:00:00

abstract：:Constitutive beta-glucosidases from Saccharomyces fragilis (Y-18) and S. dobzhanskii (Y-19) precipitated at the same concentration of ammonium sulfate. The partially purified enzymes had similar activation energies, molecular weights, affinities for certain natural and synthetic beta-glucosides, and optimal pH values ...

journal_title：Journal of bacteriology

authors： Fleming LW,Duerksen JD

更新日期：1967-01-01 00:00:00

abstract：:Survivors of nitrosoguanidine-treated cultures of a colicinogenic strain of Salmonella typhimurium were tested for spontaneous production of colicin E1. Of about 1,000 colonies tested, 13 produced no (or very narrow) colicin zones. Four of these isolates proved to be more sensitive to ultraviolet (UV) light, X rays, a...

journal_title：Journal of bacteriology

authors： MacPhee DG

更新日期：1970-10-01 00:00:00

abstract：:Seventy-one methionineless and cysteineless auxotrophs of Pseudomonas aeruginosa were placed into nine groups on the basis of their growth on methionine precursors and the cross-feeding response. Transduction experiments with bacteriophage F116 indicated the presence of four linkage groups among the methionineless mut...

journal_title：Journal of bacteriology

authors： Calhoun DH,Feary TW

更新日期：1969-01-01 00:00:00

abstract：:An ADP-ribosylating toxin named Aeromonas salmonicida exoenzyme T (AexT) in A. salmonicida subsp. salmonicida, the etiological agent of furunculosis in fish, was characterized. Gene aexT, encoding toxin AexT, was cloned and characterized by sequence analysis. AexT shows significant sequence similarity to the ExoS and ...

journal_title：Journal of bacteriology

authors： Braun M,Stuber K,Schlatter Y,Wahli T,Kuhnert P,Frey J

更新日期：2002-04-01 00:00:00

abstract：:Different electron carriers of the non-desulfoviridin-containing, sulfate-reducing bacterium Desulfovibrio desulfuricans (Norway strain) have been studied. Two nonheme iron proteins, ferredoxin and rubredoxin, have been purified. This ferredoxin contains four atoms of non-heme iron and acid-labile sulfur and six resid...

journal_title：Journal of bacteriology

authors： Bruschi M,Hatchikian CE,Golovleva LA,Gall JL

更新日期：1977-01-01 00:00:00

abstract：:Anthranilate (2-aminobenzoate) is an important intermediate in tryptophan metabolism. In order to investigate the degradation of tryptophan through anthranilate by Burkholderia cepacia, several plasposon mutations were constructed of strain DBO1 and one mutant with the plasposon insertion in the anthranilate dioxygena...

journal_title：Journal of bacteriology

authors： Chang HK,Mohseni P,Zylstra GJ

更新日期：2003-10-01 00:00:00

abstract：:The thermosensitive growth phenotype exerted by runaway-mutant plasmids was suppressed by sublethal doses of the DNA gyrase inhibitors novobiocin or nalidixic acid, although the latter drug was less efficient. A novobiocin-resistant gyrB mutant Escherichia coli strain prevented expression of the runaway phenotype at 3...

journal_title：Journal of bacteriology

authors： Uhlin BE,Nordström K

更新日期：1985-05-01 00:00:00

abstract：:A study of the distribution of pyrrolidone carboxylyl peptidase (PCP) activity among cell fractions of Streptococcus cremoris HP revealed that this enzyme is associated with a particulate fraction, which mainly consists of membrane material. This location could only be established using a gentle nonmechanical method f...

journal_title：Journal of bacteriology

authors： Exterkate FA

更新日期：1977-03-01 00:00:00

abstract：:Among 30 conjugative plasmids of enteric bacteria from 23 incompatibility (Inc) groups, we found 19 (from 12 Inc groups) which can complement defects caused by a defective single-stranded DNA-binding protein of Escherichia coli K-12. The genes which are responsible for the complementation from three of these plasmids ...

journal_title：Journal of bacteriology

authors： Golub EI,Low KB

更新日期：1985-04-01 00:00:00

abstract：:E2 (dihydrolipoamide acetyltransferase) and E3 (dihydrolipoamide dehydrogenase) of the Clostridium magnum acetoin dehydrogenase enzyme system were copurified in a three-step procedure from acetoin-grown cells. The denatured E2-E3 preparation comprised two polypeptides with M(r)s of 49,000 and 67,000, respectively. Mic...

journal_title：Journal of bacteriology

authors： Krüger N,Oppermann FB,Lorenzl H,Steinbüchel A

更新日期：1994-06-01 00:00:00

abstract：:We have previously used the bacteriophage transposon Mu d1 (which encodes the lacZY structural genes but without their promoter) to construct strains that have lacZY fused to phosphate-regulated promoters in Escherichia coli K-12. Among 18 identified phosphate-starvation-inducible (psi) genes, three (the phoA and two ...

journal_title：Journal of bacteriology

authors： Wanner BL,Wieder S,McSharry R

更新日期：1981-04-01 00:00:00

abstract：:The light organ of the squid Euprymna scolopes is specifically colonized to a high density by the marine bacterium Vibrio fischeri. To date, only a few factors contributing to the specificity of this symbiosis have been identified. Using a genetic screen for random transposon mutants defective in initiating the symbio...

journal_title：Journal of bacteriology

authors： Visick KL,Skoufos LM

更新日期：2001-02-01 00:00:00

abstract：:LaBrec, Eugene H., Herman Schneider, Thomas J. Magnani, and Samuel B. Formal. Epithelial cell penetration as an essential step in the pathogenesis of bacillary dysentery. J. Bacteriol. 88:1503-1518. 1964.-A parent strain of Shigella flexneri 2a and a colonial mutant derived from it were studied in three animal models....

journal_title：Journal of bacteriology

authors： Labrec EH,Schneider H,Magnani TJ,Formal SB

更新日期：1964-11-01 00:00:00

abstract：:Two regulatory proteins, Gal repressor and isorepressor, control the expression of the gal and mgl operons in Escherichia coli. The transcription start sites for galR and galS, the genes for the repressor and isorepressor, were determined by primer extension of in vivo transcripts. Study of the promoter-lacZ gene fusi...

journal_title：Journal of bacteriology

authors： Weickert MJ,Adhya S

更新日期：1993-01-01 00:00:00

abstract：:Streptococcus agalactiae (group B streptococcus [GBS]) is a major cause of infections in newborns, pregnant women, and immunocompromised patients. GBS strain CNCTC10/84 is a clinical isolate that has high virulence in animal models of infection and has been used extensively to study GBS pathogenesis. Two unusual featu...

journal_title：Journal of bacteriology

authors： Zhu L,Beres SB,Yerramilli P,Pruitt L,Cantu CC,Olsen RJ,Musser JM

更新日期：2020-11-04 00:00:00

abstract：:West Indian spiny lobsters, Panulirus argus, synthesized a hemolymph bactericidin after being injected with killed suspensions of gram-negative bacillus EMB-1 isolated from the normal gut of this lobster. To study differences between the primary response and secondary response, animals were given a primary antigen inj...

journal_title：Journal of bacteriology

authors： Evans EE,Weinheimer PF,Painter B,Acton RT,Evans ML

更新日期：1969-06-01 00:00:00

abstract：:In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its proteolysis. Here, we exa...

journal_title：Journal of bacteriology

authors： Chen YE,Tsokos CG,Biondi EG,Perchuk BS,Laub MT

更新日期：2009-12-01 00:00:00

abstract：:Mutant strains of Escherichia coli K-12 were isolated possessing mutations which affected the tyrosine-inhibitable 3-deoxy-d-arabinoheptulosonic acid-7-phosphate (DAHP) synthetase, the phenylalanine-inhibitable DAHP synthetase, or the tryptophan-repressible DAHP synthetase. The mutations causing the loss of each of th...

journal_title：Journal of bacteriology

authors： Wallace BJ,Pittard J

更新日期：1967-01-01 00:00:00