Abstract:
:In Caulobacter crescentus, progression through the cell cycle is governed by the periodic activation and inactivation of the master regulator CtrA. Two phosphorelays, each initiating with the histidine kinase CckA, promote CtrA activation by driving its phosphorylation and by inactivating its proteolysis. Here, we examined whether the CckA phosphorelays also influence the downregulation of CtrA. We demonstrate that CckA is bifunctional, capable of acting as either a kinase or phosphatase to drive the activation or inactivation, respectively, of CtrA. By identifying mutations that uncouple these two activities, we show that CckA's phosphatase activity is important for downregulating CtrA prior to DNA replication initiation in vivo but that other phosphatases may exist. Our results demonstrate that cell cycle transitions in Caulobacter require and are likely driven by the toggling of CckA between its kinase and phosphatase states. More generally, our results emphasize how the bifunctional nature of histidine kinases can help switch cells between mutually exclusive states.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Chen YE,Tsokos CG,Biondi EG,Perchuk BS,Laub MTdoi
10.1128/JB.00992-09subject
Has Abstractpub_date
2009-12-01 00:00:00pages
7417-29issue
24eissn
0021-9193issn
1098-5530pii
JB.00992-09journal_volume
191pub_type
杂志文章abstract::The model archaeon Pyrococcus furiosus grows optimally near 100°C on carbohydrates and peptides. Its genome sequence (NCBI) was determined 12 years ago. A genetically tractable strain, COM1, was very recently reported, and here we describe its genome sequence. Of 1,909,827 bp in size, it is 1,571 bp longer (0.1%) than...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00439-12
更新日期:2012-08-01 00:00:00
abstract::The major cold shock protein of Escherichia coli, CS7.4, is produced at a level of 13% of total protein synthesis upon a temperature shift from 37 to 10 degrees C. The transcription of its gene (cspA) was found to be tightly regulated and induced only at low temperature. In addition, the cspA mRNA was extremely unstab...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.12.3867-3873.1992
更新日期:1992-06-01 00:00:00
abstract::When an asporogenic diploid yeast, Candida tropicalis, is cultivated on n-alkane, the expression of the genes encoding enzymes of the peroxisomal beta-oxidation pathway is highly induced. An upstream activation sequence (UAS) which can induce transcription in response to n-alkane (UAS(ALK)) was identified on the promo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.9.2492-2497.2000
更新日期:2000-05-01 00:00:00
abstract::Evidence is reported here that alkalinization of Clostridium acetobutylicum cytoplasm involves hydrogenase activity. A decrease of in vivo hydrogenase activity is accompanied by intracellular accumulation of protons leading to a negative (interior acidic) pH gradient. However, the organism is able to maintain a consta...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.19.6146-6147.1994
更新日期:1994-10-01 00:00:00
abstract::Hancock, R. (Harvard Medical School, Boston, Mass.). Early effects of streptomycin on Bacillus megaterium. J. Bacteriol. 88:633-639. 1964.-The sequence of some early effects of streptomycin on Bacillus megaterium was investigated. An increase in the rate of efflux of cellular K ions preceded loss of viability, but eff...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.3.633-639.1964
更新日期:1964-09-01 00:00:00
abstract:UNLABELLED:Current knowledge regarding the mechanism that governs flagellar motor rotation in response to environmental stimuli stems mainly from the study of monotrichous and peritrichous bacteria. Little is known about how two polar flagella, one at each cell pole of the so-called amphitrichous bacterium, are coordin...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00172-15
更新日期:2015-10-01 00:00:00
abstract::The lethal and mutagenic effects of various mutagens on Neisseria gonorrhoeae were investigated. Lethality studies demonstrated that N. gonorrhoeae was relatively sensitive to ethyl methanesulfonate, UV light, and methyl methanesulfonate. Although N. gonorrhoeae was readily mutated by ethyl methanesulfonate and N-meth...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.288-293.1984
更新日期:1984-10-01 00:00:00
abstract::Gram-negative bacteria acquire ferric siderophores through TonB-dependent outer membrane transporters (TBDT). By fluorescence spectroscopic hgh-throughput screening (FLHTS), we identified inhibitors of TonB-dependent ferric enterobactin (FeEnt) uptake through Escherichia coli FepA (EcoFepA). Among 165 inhibitors found...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00889-16
更新日期:2017-04-25 00:00:00
abstract::Addition of purines to the growth medium of Escherichia coli represses synthesis of cytosine deaminase (codA) and enzymes of purine de novo synthesis. After Tn10 mutagenesis, mutants displaying derepressed levels of cytosine deaminase in the presence of hypoxanthine were isolated. One of these had simultaneously acqui...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.4.2124-2127.1989
更新日期:1989-04-01 00:00:00
abstract::Biofilms contribute to virulence of Staphylococcus aureus. Formation of biofilms is multifactorial, involving polysaccharide, protein, and DNA components, which are controlled by various regulators. Here we report that deletion of the rsp gene resulted in an increase in biofilm formation in strain MW2, suggesting that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05454-11
更新日期:2011-10-01 00:00:00
abstract:UNLABELLED:To stabilize cellular integrity in the face of environmental perturbations, most bacteria, including cyanobacteria, synthesize and maintain a strong, flexible, three-dimensional peptidoglycan lattice. Anabaena sp. strain PCC 7120 is a filamentous cyanobacterium capable of differentiating morphologically dist...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01027-15
更新日期:2016-03-31 00:00:00
abstract::Myxococcus xanthus mutants resistant to a clear-plaque derivative of phage Mx8 were isolated. A significant fraction of the mutants, easily recognizable by their colony morphology, were induced by the presence of the phage and may correspond to low-frequency lysogens. They were all defective in cell motility and showe...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.2.818-821.1984
更新日期:1984-11-01 00:00:00
abstract::Penicillin-binding protein 2 (PBP-2) of Escherichia coli K-12 was purified by covalent affinity chromatography using 6-aminopenicillanic acid covalently coupled to carboxymethyl-Sepharose (6-APA-CM-Sepharose). Purification of PBP-2 was accomplished by prebinding the methoxy cephalosporin, cefoxitin, to the Triton X-10...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.145.1.398-403.1981
更新日期:1981-01-01 00:00:00
abstract::During the growth of Clostridium cellulolyticum in chemostat cultures with ammonia as the growth-limiting nutrient, as much as 30% of the original cellobiose consumed by C. cellulolyticum was converted to cellotriose, glycogen, and polysaccharides regardless of the specific growth rates. Whereas the specific consumpti...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.7.2010-2017.2000
更新日期:2000-04-01 00:00:00
abstract::Cores are large, rod-shaped structures that have been found almost exclusively in group D streptococci, measure 0.1 to 0.16 mum in diameter, and extend the width or length of cells. This study has shown that cores are produced in the cells at a reproducible point in early stationary growth after extensive mesosomal fo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.1.445-456.1977
更新日期:1977-01-01 00:00:00
abstract::The occurrence of the novel regulatory nucleotide bis(3',5')-cyclic diguanylic acid (c-di-GMP) and its relation to cellulose biogenesis in the plant pathogen Agrobacterium tumefaciens was studied. c-di-GMP was detected in acid extracts of 32P-labeled cells grown in various media, and an enzyme responsible for its form...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.12.6649-6655.1989
更新日期:1989-12-01 00:00:00
abstract::Sagers, Richard D. (Brigham Young University, Provo, Utah), Moshe Benziman, and Sigrid M. Klein. Failure of arsenate to uncouple the phosphotransacetylase system in Clostridium acidiurici. J. Bacteriol. 86:978-984. 1963.-The conversion of pyruvate to acetyl phosphate by extracts of Clostridium acidiurici required coen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.5.978-984.1963
更新日期:1963-11-01 00:00:00
abstract::Deletion of bacterial DNA fragments is stimulated in induced Mucts62 lysogens. The host genes located proximally to the prophage are more frequently lost than those which are unlinked to the Mu genome. Genes located on either side of a Mu genome are deleted in the same manner. Like the other Mu-induced rearrangements,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.2.477-483.1978
更新日期:1978-11-01 00:00:00
abstract::The dimorphic fungus Mucor racemosus was found to contain the cyclic nucleotide guanosine 3',5'-monophosphate (cGMP). Approximately equivalent amounts of the compound were found in ungerminated spores, yeastlike cells, and mycelia. Germinating spores contained severalfold higher amounts of cGMP than the other cell for...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.1226-1228.1976
更新日期:1976-03-01 00:00:00
abstract::A series of electron micrographs showing the presence of different molecular forms representing various replication stages of plasmid deoxyribonucleic acid from Streptomyces griseus was obtained. Based upon an analysis of these electron micrographs, a tentative model for plasmid deoxyribonucleic acid replication in S....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.1.412-414.1981
更新日期:1981-04-01 00:00:00
abstract::The Escherichia coli flaA gene product (also called cheC) plays a crucial role in switching flagellar rotational direction during chemotactic responses. Wild-type and mutant alleles have been cloned onto plasmid vectors, and the gene product has been identified as a 37,000-dalton protein. The flaA product appeared as ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.398-405.1985
更新日期:1985-04-01 00:00:00
abstract::Pseudomonas syringae pv. tabaci 6605 possesses a genetic region involved in flagellin glycosylation. This region is composed of three open reading frames: orf1, orf2, and orf3. Our previous study revealed that orf1 and orf2 encode glycosyltransferases; on the other hand, orf3 has no role in posttranslational modificat...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00763-06
更新日期:2006-12-01 00:00:00
abstract::The ops and tps genes of Myxococcus xanthus have ca. 90% DNA and amino acid sequence homology and are in the same orientation separated by a spacer region of only 1.4 kilobases. The products of the two genes were found to cross-react immunologically, and both were capable of Ca2+-dependent self-assembly on the surface...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.163.1.121-125.1985
更新日期:1985-07-01 00:00:00
abstract::The Alcaligenes eutrophus JMP134 plasmid pJP4 contains genes necessary for the complete degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) and 3-chlorobenzoic acid. tfdA encodes 2,4-D monooxygenase, the initial enzyme in the 2,4-D catabolic pathway. The tfdA locus has recently been localized to a region on pJP4 13 ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.12.5669-5672.1988
更新日期:1988-12-01 00:00:00
abstract::Covalently closed circular deoxyribonucleic acid (DNA) was isolated from two strains of Streptomyces coelicolor A3(2), representing two of the known fertility types. In each of the two strains circular DNA of about 20 times 10-6 daltons could be detected, amounting to about 1.5% of the total cellular DNA. The possible...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.121.2.416-421.1975
更新日期:1975-02-01 00:00:00
abstract::Proteomics and targeted gene disruption were used to investigate the catabolism of benzene, styrene, biphenyl, and ethylbenzene in Rhodococcus jostii RHA1, a well-studied soil bacterium whose potent polychlorinated biphenyl (PCB)-transforming properties are partly due to the presence of the related Bph and Etb pathway...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01122-07
更新日期:2008-01-01 00:00:00
abstract::The phosphomannose isomerase (pmi) gene of Escherichia coli was cloned on a broad-host-range cosmid vector and expressed in Pseudomonas aeruginosa at a low level. Plasmid pAD3, which harbors the E. coli pmi gene, contains a 6.2-kilobase-pair HindIII fragment derived from the chromosome of E. coli. Subcloning produced ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.161.1.249-257.1985
更新日期:1985-01-01 00:00:00
abstract::Mutants of Agrobacterium tumefaciens requiring methionine for growth on a solid basal medium were induced by the use of N-methyl-N'-nitro-N-nitrosoguanidine. In addition to the difference of mutant strains, the extent of methionine dependency differed in a liquid basal medium and in the presence of aspartate or fumara...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.6.2078-2082.1968
更新日期:1968-06-01 00:00:00
abstract::Nisin is a pore-forming antimicrobial peptide. The capacity of nisin to induce transmembrane movement of a fluorescent phospholipid in lipid vesicles was investigated. Unilamellar phospholipid vesicles that contained a fluorescent phospholipid (1-acyl-2-(6-[(7-nitro-2-1, 3-benzoxadiazol-4-yl)amino]caproyl)-sn-glycero-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.180.24.6565-6570.1998
更新日期:1998-12-01 00:00:00
abstract::The ability of deoxyribonucleic acid (DNA) isolated from a number of actinomycetes to reassociate with reference DNA from Actinoplanes philippinensis or Streptomyces venezuelae has been measured. All of the DNA preparations except for those from Nocardia erythropolis and Thermomonospora viridis contained 70 to 73 mole...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.102.1.30-35.1970
更新日期:1970-04-01 00:00:00