Abstract:
:Xylella fastidiosa is a xylem-limited nonflagellated bacterium that causes economically important diseases of plants by developing biofilms that block xylem sap flow. How the bacterium is translocated downward in the host plant's vascular system against the direction of the transpiration stream has long been a puzzling phenomenon. Using microfabricated chambers designed to mimic some of the features of xylem vessels, we discovered that X. fastidiosa migrates via type IV-pilus-mediated twitching motility at speeds up to 5 mum min(-1) against a rapidly flowing medium (20,000 mum min(-1)). Electron microscopy revealed that there are two length classes of pili, long type IV pili (1.0 to 5.8 mum) and short type I pili (0.4 to 1.0 mum). We further demonstrated that two knockout mutants (pilB and pilQ mutants) that are deficient in type IV pili do not twitch and are inhibited from colonizing upstream vascular regions in planta. In addition, mutants with insertions in pilB or pilQ (possessing type I pili only) express enhanced biofilm formation, whereas a mutant with an insertion in fimA (possessing only type IV pili) is biofilm deficient.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Meng Y,Li Y,Galvani CD,Hao G,Turner JN,Burr TJ,Hoch HCdoi
10.1128/JB.187.16.5560-5567.2005keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
5560-7issue
16eissn
0021-9193issn
1098-5530pii
187/16/5560journal_volume
187pub_type
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doi:10.1128/JB.101.1.160-165.1970
更新日期:1970-01-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1989-06-01 00:00:00
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更新日期:1963-12-01 00:00:00
abstract::The phoB gene of Bacillus subtilis encodes an alkaline phosphatase (PhoB, formerly alkaline phosphatase III) that is expressed from separate promoters during phosphate deprivation in a PhoP-PhoR-dependent manner and at stage two of sporulation under phosphate-sufficient conditions independent of PhoP-PhoR. Isogenic st...
journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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更新日期:2003-08-01 00:00:00
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更新日期:1986-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.5.1480-1483.1996
更新日期:1996-03-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1992-09-01 00:00:00
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journal_title:Journal of bacteriology
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更新日期:1968-10-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
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更新日期:2003-02-01 00:00:00
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doi:10.1128/JB.154.2.743-747.1983
更新日期:1983-05-01 00:00:00
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更新日期:1984-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.181.19.5898-5908.1999
更新日期:1999-10-01 00:00:00
abstract::Bacteroides ruminicola B(1)4, a noncellulolytic rumen bacterium, produces an endoglucanase (carboxymethylcellulase [CMCase]) that is excreted into the culture supernatant. Cultures grown on glucose, fructose, maltose, mannose, and cellobiose had high specific activities of CMCase (greater than 3 mmol of reducing sugar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:1990-07-01 00:00:00
abstract::Biofilms contribute to virulence of Staphylococcus aureus. Formation of biofilms is multifactorial, involving polysaccharide, protein, and DNA components, which are controlled by various regulators. Here we report that deletion of the rsp gene resulted in an increase in biofilm formation in strain MW2, suggesting that...
journal_title:Journal of bacteriology
pub_type: 杂志文章
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更新日期:2011-10-01 00:00:00
abstract::Salmonella typhimurium contains a transport system for sn-glycerol-3-phosphate that is inducible by growth on glycerol and sn-glycerol-3-phosphate. In fully induced cells, the system exhibited an apparent Km of 50 microM and a Vmax of 2.2 nmol/min . 10(8) cells. The corresponding system in Escherichia coli exhibits, u...
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pub_type: 杂志文章
doi:10.1128/JB.155.1.186-195.1983
更新日期:1983-07-01 00:00:00