Mutations in the araC regulatory gene of Escherichia coli B/r that affect repressor and activator functions of AraC protein.

Abstract:

:Mutations in the araC gene of Escherichia coli B/r were isolated which alter both activation of the araBAD operon expression and autoregulation. The mutations were isolated on an araC-containing plasmid by hydroxylamine mutagenesis of plasmid DNA. The mutant phenotype selected was the inability to autoregulate. The DNA sequence of 16 mutants was determined and found to consist of seven different missense mutations located within the distal third of the araC gene. Enzyme activities revealed that each araC mutation had altered both autoregulatory and activator functions of AraC protein. The mutational analysis presented in this paper suggests that both autoregulatory and activator functions are localized to the same determinants of the AraC protein and that the amino acid sequence within the carboxy-terminal region of AraC protein is important for site-specific DNA binding.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Cass LG,Wilcox G

doi

10.1128/jb.166.3.892-900.1986

subject

Has Abstract

pub_date

1986-06-01 00:00:00

pages

892-900

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

166

pub_type

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