Abstract:
:Mutations in the araC gene of Escherichia coli B/r were isolated which alter both activation of the araBAD operon expression and autoregulation. The mutations were isolated on an araC-containing plasmid by hydroxylamine mutagenesis of plasmid DNA. The mutant phenotype selected was the inability to autoregulate. The DNA sequence of 16 mutants was determined and found to consist of seven different missense mutations located within the distal third of the araC gene. Enzyme activities revealed that each araC mutation had altered both autoregulatory and activator functions of AraC protein. The mutational analysis presented in this paper suggests that both autoregulatory and activator functions are localized to the same determinants of the AraC protein and that the amino acid sequence within the carboxy-terminal region of AraC protein is important for site-specific DNA binding.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Cass LG,Wilcox Gdoi
10.1128/jb.166.3.892-900.1986subject
Has Abstractpub_date
1986-06-01 00:00:00pages
892-900issue
3eissn
0021-9193issn
1098-5530journal_volume
166pub_type
杂志文章abstract::The luminescence (lux) operon (luxICDABEG) of the symbiotic bacterium Vibrio fischeri is regulated by the transcriptional activator LuxR and two acyl-homoserine lactone (acyl-HSL) autoinducers (the luxI-dependent 3-oxo-hexanoyl-HSL [3-oxo-C6-HSL] and the ainS-dependent octanoyl-HSL [C8-HSL]) in a population density-re...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.10.2811-2822.2000
更新日期:2000-05-01 00:00:00
abstract::Expression of virulence factors by Bordetella pertussis is altered by environmental signals (antigenic modulation) and is dependent on an activator encoded by a gene called vir. We have used TnphoA (Tn5 IS50L::phoA) gene fusions to define two sets of genes whose expression is either activated (vag loci) or repressed (...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.11.5059-5066.1988
更新日期:1988-11-01 00:00:00
abstract::The poly-beta-hydroxybutyrate (PHB) biosynthetic pathway from Alcaligenes eutrophus H16 has been cloned and expressed in Escherichia coli. Initially, an A. eutrophus H16 genomic library was constructed by using cosmid pVK102, and cosmid clones that encoded the PHB biosynthetic pathway were sought by assaying for the f...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.10.4431-4436.1988
更新日期:1988-10-01 00:00:00
abstract::The major facilitator superfamily includes a large collection of evolutionarily related proteins that have been implicated in the transport of a variety of solutes and metabolites across the membranes of organisms ranging from bacteria to humans. We have recently reported the three-dimensional structure, at 6.5 A reso...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.5.1712-1718.2003
更新日期:2003-03-01 00:00:00
abstract::Salmonella enteritidis was injected intravenously, intraperitoneally, or subcutaneously into specific pathogen-free mice. The number of organisms in the blood, liver, spleen, peritoneal cavity, and draining inguinal lymph node was determined by daily enumeration. Opsonization of the organism with hyperimmune serum inc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.97.2.667-675.1969
更新日期:1969-02-01 00:00:00
abstract::Accumulation of l-alpha-glycerophosphate, in cells of Bacillus subtilis mutants lacking the nicotinamide adenine dinucleotide-independent glycerophosphate dehydrogenase activity, suppresses both growth and sporulation. After growth has stopped, the cells slowly develop one and later more asymmetric septa that are thic...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.113.2.1034-1045.1973
更新日期:1973-02-01 00:00:00
abstract::Acetate kinase (ACK) converts acetyl phosphate to acetate along with the generation of ATP in the pathway for mixed-acid fermentation in Lactococcus lactis. The reverse reaction yields acetyl phosphate for assimilation purposes. Remarkably, L. lactis has two ACK isozymes, and the corresponding genes are present in an ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01277-13
更新日期:2014-04-01 00:00:00
abstract::A completely stable F32 merodiploid has been isolated, which has the dsd genotype dsdA(+)dsdCx3 /F -dsdA(+)dsdC(+). The stabilization apparently does not result from an alteration in the F, or from its integration in the chromosome, but rather from a chromosomal aberration. The stable strain can exist in two forms, wh...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.6.1989-1993.1967
更新日期:1967-12-01 00:00:00
abstract::The gap-2 gene, encoding the NAD(P)-dependent D-glyceraldehyde-3-phosphate dehydrogenase (GAPDH2) of the cyanobacterium Synechocystis sp. strain PCC 6803, was cloned by functional complementation of an Escherichia coli gap mutant with a genomic DNA library; this is the first time that this cloning strategy has been us...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.14.4513-4522.1997
更新日期:1997-07-01 00:00:00
abstract::Degradation of 2-hydroxy-5-carboxymethylmuconic semialdehyde, the ring fission product of the 4-hydroxyphenylacetate meta-cleavage pathway, by mutant strains P23X19 and P23X16 of Pseudomonas putida NCI B 9865 was studied. Both mutants were unable to grow on either 4-hydroxyphenylacetate of 3,4-dihydroxyphenylacetate. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.142.2.480-485.1980
更新日期:1980-05-01 00:00:00
abstract::Sequences of the major outer membrane protein (MOMP) gene (ompA) and the outer membrane complex B protein gene (omcB) from Chlamydia trachomatis, Chlamydia pneumoniae, and Chlamydia psittaci were analyzed for evidence of intragenic recombination and for linkage equilibrium. The Sawyer runs test, compatibility matrices...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.20.5997-6008.2001
更新日期:2001-10-01 00:00:00
abstract::The amounts of glycogen and trehalose have been measured in cells of a prototrophic diploid yeast strain subjected to a variety of nutrient limitations. Both glycogen and trehalose were accumulated in cells deprived specifically of nirogen, sulfur, or phosphorus, suggesting that reserve carbohydrate accumulation is a ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.143.3.1384-1394.1980
更新日期:1980-09-01 00:00:00
abstract::The genome of Halobacterium sp. strain NRC-1 contains a large gene cluster, gvpMLKJIHGFEDACNO, that is both necessary and sufficient for the production of buoyant gas-filled vesicles. Due to the resistance of gas vesicles to solubilization, only the major gas vesicle protein GvpA and a single minor protein, GvpC, were...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.186.10.3182-3186.2004
更新日期:2004-05-01 00:00:00
abstract::Certain group VIIIB transition metal compounds were found to inhibit cell division in Escherichia coli, causing marked filamentous growth. Gram-negative bacilli were the most sensitive to this effect, whereas gram-positive bacilli responded only at near-toxic levels of the metal. None of the cocci tested showed any ap...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.2.716-721.1967
更新日期:1967-02-01 00:00:00
abstract::The Bacillus subtilis PurR mediates adenine repression and guanosine induction of purA. PRPP inhibits binding of PurR to DNA in vitro. Mutations in the PRPP binding motif of PurR caused strong repression regardless of purine exclusions or additions, establishing the role of PRPP as regulator of PurR. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.22.6728-6731.2003
更新日期:2003-11-01 00:00:00
abstract::Dienes, Louis (Massachusetts General Hospital, Boston, Mass.), Bojana M. Bandur, and Sarabelle Madoff. Development of L-type growth in Neisseria gonorrhoeae cultures. J. Bacteriol. 87:1471-1476. 1964.-Strains of Neisseria gonorrhoeae (22) were studied immediately after isolation from patients. After 24 to 48 hr of inc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.87.6.1471-1476.1964
更新日期:1964-06-01 00:00:00
abstract::Burkholderia pseudomallei, the causative agent of melioidosis, contains a large pathogen genome (7.2 Mb) with ∼2,000 genes of putative or unknown function. Interactions with potential hosts and environmental factors may induce rapid adaptations in these B. pseudomallei genes, which can be discerned through evolutionar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00718-13
更新日期:2013-12-01 00:00:00
abstract::Here we show that the type III secretion gatekeeper protein SepL resembles an aberrant effector protein in binding to a class 1 type III secretion chaperone (Orf12, here renamed CesL). We also show that short N-terminal fragments (≤70 amino acids) from SepL are capable of targeting fusion proteins for secretion and tr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00760-10
更新日期:2010-11-01 00:00:00
abstract::The Tol-Pal system of Escherichia coli is involved in maintaining outer membrane stability. Mutations in tolQ, tolR, tolA, tolB, or pal genes result in sensitivity to bile salts and the leakage of periplasmic proteins. Moreover, some of the tol genes are necessary for the entry of group A colicins and the DNA of filam...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.3.821-824.2000
更新日期:2000-02-01 00:00:00
abstract::The peptidoglycan cell wall determines the shape and structural integrity of a bacterial cell. Class B penicillin-binding proteins (PBPs) carry a transpeptidase activity that cross-links peptidoglycan strands via their peptide side chains, and some of these proteins are directly involved in cell shape determination. N...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.16.4717-4726.2003
更新日期:2003-08-01 00:00:00
abstract::The regulatory region controlling the expression of tetracycline resistance and repressor genes contains two nearly identical regions of dyad symmetry. Deletions of this control region were isolated by digestion with S1 nuclease. The ability of these deletions to bind the tet repressor was determined by an in vivo rep...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.156.3.1188-1191.1983
更新日期:1983-12-01 00:00:00
abstract::Mutants of the fhuA gene of Escherichia coli K-12, which encodes a receptor protein in the outer membrane, took up ferrichrome after exposure to pronase, whereas fhuB mutants remained transport negative. The latter finding supports our previous proposal that fhuB mutants are defective in a function that residues in th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.146.3.1158-1161.1981
更新日期:1981-06-01 00:00:00
abstract::A 6.9-kilobase region of the Escherichia coli F plasmid containing the 3' half of the traD gene and the entire traI gene (encodes the TraI protein, DNA helicase I and TraI, a polypeptide arising from an internal in-frame translational start in traI) has been sequenced. A previously unidentified open reading frame (ten...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.4127-4131.1990
更新日期:1990-07-01 00:00:00
abstract::The asg mutants of Myxococcus xanthus are defective in production of extracellular A-signal, which serves as a cell density signal for fruiting-body development. The DNA sequence of asgB, one of the three asg genes, was determined. The deduced amino acid sequence of AsgB contains a DNA-binding helix-turn-helix motif n...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.7.2013-2020.1994
更新日期:1994-04-01 00:00:00
abstract::A 3-hydroxypropionate/4-hydroxybutyrate cycle operates in autotrophic CO(2) fixation in various Crenarchaea, as studied in some detail in Metallosphaera sedula. This cycle and the autotrophic 3-hydroxypropionate cycle in Chloroflexus aurantiacus have in common the conversion of acetyl-coenzyme A (CoA) and two bicarbon...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00068-09
更新日期:2009-07-01 00:00:00
abstract::The motility of Spirillum volutans is caused by the rotation of each polar flagellar fascicle in a direction opposite to that of the more slowly rotating cell. Both flagella form cones of revolution oriented in the same direction. When the cell reverses its motion, both fascicles simultaneously reverse their rotation ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1431-1436.1967
更新日期:1967-11-01 00:00:00
abstract::Shapira, Jacob (Consolidated Veterans Administration Hospital, Little Rock, Ark.), Lois Lowden, and Ralph Hale. Inhibition of a thymine-deficient mutant of Escherichia coli by 5-substituted uracils. J. Bacteriol. 83:919-923. 1962.-Small inocula of well-washed cells of a thymine-requiring mutant of Escherichia coli wer...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.83.4.919-923.1962
更新日期:1962-04-01 00:00:00
abstract::The effects of surfaces on the physiology of bacteria adhering to surfaces or immobilized within biofilms are receiving more interest. A study of the effects of hydrophobic and hydrophilic substrata on the colonization behavior of a marine bacterium, SW5, revealed major differences in the morphology of SW5 on these su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.22.6900-6906.1994
更新日期:1994-11-01 00:00:00
abstract::Replicating DNA molecules of the nonconjugative R plasmid RSF1010 (Smr Sur) were cleaved with the EcoRI restriction endonuclease and examined with the electron microscope. Results of this analysis indicated that replication is initiated from an origin located at about 19% of total genome size from one of the EcoRI end...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.134.3.1117-1122.1978
更新日期:1978-06-01 00:00:00
abstract::Peptide inhibitors of phage lambda site-specific recombination were previously isolated by screening synthetic combinatorial peptide libraries. These inhibitors cause the accumulation of complexes between the recombinase and the Holliday junction intermediate of several highly divergent tyrosine recombinases. Peptide ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01559-08
更新日期:2009-04-01 00:00:00