Time-resolved determination of the CcpA regulon of Lactococcus lactis subsp. cremoris MG1363.

abstract：:This paper describes the regulation of a transfer ribonucleic acid (tRNA) biosynthetic enzyme, the tRNA(m5U)methyltransferase (EC 2.1.1.35). This enzyme catalyzes the formation of 5-methyluridine (m5U, ribothymidine) in all tRNA chains of Escherichia coli. Partial deprivation of charged tRNAVal can be imposed by shift...

journal_title：Journal of bacteriology

authors： Ny T,Björk GR

更新日期：1977-05-01 00:00:00

abstract：:A procedure for protoplast formation in Escherichia coli is described. Removal of the cell wall was confirmed by examination of cells in thin-section preparations. ...

journal_title：Journal of bacteriology

authors： Weiss RL

更新日期：1976-11-01 00:00:00

abstract：:In Escherichia coli K-12, two enzymes, encoded by ilvE and tyrB, catalyze the amination of 2-ketoisocaproate (2-KIC) to form leucine. Although leucine-requiring derivatives of an ilvE strain that are unable to grow on 2-KIC were expected to have mutations only in tyrB, mapping studies showed that one such mutation was...

journal_title：Journal of bacteriology

authors： Vartak NB,Liu L,Wang BM,Berg CM

更新日期：1991-06-01 00:00:00

abstract：:Gram-negative bacteria acquire ferric siderophores through TonB-dependent outer membrane transporters (TBDT). By fluorescence spectroscopic hgh-throughput screening (FLHTS), we identified inhibitors of TonB-dependent ferric enterobactin (FeEnt) uptake through Escherichia coli FepA (EcoFepA). Among 165 inhibitors found...

journal_title：Journal of bacteriology

authors： Nairn BL,Eliasson OS,Hyder DR,Long NJ,Majumdar A,Chakravorty S,McDonald P,Roy A,Newton SM,Klebba PE

更新日期：2017-04-25 00:00:00

abstract：:The RecA proteins of the unusually strong protease-constitutive mutants recA1202 and recA1211 can use RNA in addition to single-stranded DNA (ssDNA) as a cofactor in the cleavage of the LexA repressor in vitro. In the presence of rRNA or tRNA, the effectiveness of these proteins decreased in the order RecA1202 greater...

journal_title：Journal of bacteriology

authors： Wang WB,Tessman ES,Tessman I

更新日期：1988-10-01 00:00:00

abstract：:The role of the DNA primase of IncP plasmids was examined with a derivative of RP4 containing Tn7 in the primase gene (pri). The mutant was defective in mediating bacterial conjugation, with the deficiency varying according to the bacterial strains used as donors and recipients. Complementation tests involving recombi...

journal_title：Journal of bacteriology

authors： Merryweather A,Barth PT,Wilkins BM

更新日期：1986-07-01 00:00:00

abstract：:A Synechocystis sp. strain PCC 6803 mutant lacking CtaI, a main subunit of cytochrome c oxidase, is not capable of growing at light intensities below 5 micromol photons m(-2) s(-1), presumably due to an overreduced plastoquinone pool in the thylakoid membrane. Upon selection for growth at light intensities below 5 mic...

journal_title：Journal of bacteriology

authors： Kufryk GI,Vermaas WF

更新日期：2006-02-01 00:00:00

abstract：:A large number of carbon source utilization pathways are repressed in Bacillus subtilis by the global regulator CcpA, which also acts as an activator of carbon excretion pathways during growth in media containing glucose. In this study, CcpA mutants defective in transcriptional activation of the alsSD operon, which is...

journal_title：Journal of bacteriology

authors： Turinsky AJ,Moir-Blais TR,Grundy FJ,Henkin TM

更新日期：2000-10-01 00:00:00

abstract：:Plasma cells obtained from the peripheral blood of a patient with multiple myeloma was incubated in serum and Krebs-Ringer bicarbonate buffer with (14)C-labeled glucose, acetate, and propionate. Glucose utilization by these cells amounted to 0.5 mumole per hr per 10(8) cells and was mainly via the Embden-Meyerhof path...

journal_title：Journal of bacteriology

authors： Stjernholm RL

更新日期：1967-05-01 00:00:00

abstract：:Heating at 55 C causes the intracellular degradation of ribosomes of Staphylococcus aureus MF-31. The 30S subunit of heated cells appears to be selectively attacked. Analysis of ribonucleic acid (RNA) showed that 16S RNA was destroyed and that the secondary structure of 23S RNA was altered. ...

journal_title：Journal of bacteriology

authors： Rosenthal LJ,Iandolo JJ

更新日期：1970-09-01 00:00:00

abstract：:The conjugation system of the IncP alpha plasmid RK2/RP4 is encoded by transfer regions designated Tra1, Tra2, and Tra3. The Tra1 core region, cloned on plasmid pDG4 delta 22, consists of the origin of transfer (oriT) and 2.6 kilobases of flanking DNA providing IncP alpha plasmid-specific functions that allow pDG4 del...

journal_title：Journal of bacteriology

authors： Guiney DG,Deiss C,Simnad V,Yee L,Pansegrau W,Lanka E

更新日期：1989-07-01 00:00:00

abstract：:Many bacterial species produce capsular polysaccharides that contribute to pathogenesis through evasion of the host innate immune system. The gram-positive pathogen Enterococcus faecalis was previously reported to produce one of four capsule serotypes (A, B, C, or D). Previous studies describing the four capsule serot...

journal_title：Journal of bacteriology

authors： Thurlow LR,Thomas VC,Hancock LE

更新日期：2009-10-01 00:00:00

abstract：:Chorpenning, Frank W. (The Ohio State University, Columbus), and Matthew C. Dodd. Heterogenetic antigens of gram-positive bacteria. J. Bacteriol. 91:1440-1445. 1966.-Soluble antigens obtained by various methods from gram-positive bacteria were used to modify erythrocytes whose hemagglutinating reactions with immune ra...

journal_title：Journal of bacteriology

authors： Chorpenning FW,Dodd MC

更新日期：1966-04-01 00:00:00

abstract：:Streptomyces azureus carried one copy or less of plasmid pSA1, which elicited pocks at 0.1 to 1.0%. Strain PK100 was isolated from the wild-type strain after UV irradiation. PK100 carried approximately 20 to 30 copies of pSA1.1, a derivative of pSA1. Plasmid pSA1.1 elicited pocks at 100% and inhibited spore and thiost...

journal_title：Journal of bacteriology

authors： Miyoshi YK,Ogata S,Hayashida S

更新日期：1986-10-01 00:00:00

abstract：:Rubrerythrin was purified by multistep chromatography under anaerobic, reducing conditions from the hyperthermophilic archaeon Pyrococcus furiosus. It is a homodimer with a molecular mass of 39.2 kDa and contains 2.9 +/- 0.2 iron atoms per subunit. The purified protein had peroxidase activity at 85 degrees C using hyd...

journal_title：Journal of bacteriology

authors： Weinberg MV,Jenney FE Jr,Cui X,Adams MW

更新日期：2004-12-01 00:00:00

abstract：:Cybulska, Janina (State Institute of Hygiene, Warsaw, Poland), and J. Jeljaszewicz. Bacteriostatic activity of serum against staphylococci. J. Bacteriol. 91:953-962. 1966.-Antistaphylococcal activity of normal serum against strains exhibiting various patterns of coagulase, clumping-factor, and staphylokinase productio...

journal_title：Journal of bacteriology

authors： Cybulska J,Jeljaszewicz J

更新日期：1966-03-01 00:00:00

abstract：:Klebsiella strain RE1544 contains two lac operons, one on the chromosome and one on a lac plasmid. A mutant of RE1544, in which the lacZ genes of both operons produce no active enzyme, was found to synthesize a beta-galactosidase that hydrolyzes ortho-nitrophenyl-beta-D-galactopyranoside but not lactose. Synthesis of ...

journal_title：Journal of bacteriology

authors： Hall BG,Reeve EC

更新日期：1977-10-01 00:00:00

abstract：:Sbarra, Anthony J. (St. Margaret's Hospital, Boston, Mass.) and William Shirley. Phagocytosis inhibition and reversal. I. Effect of glycolytic intermediates and nucleotides on particle uptake. J. Bacteriol. 86:259-265. 1963.-By microscopically monitoring phagocytosis and following the biochemical changes associated wi...

journal_title：Journal of bacteriology

authors： SBARRA AJ,SHIRLEY W

更新日期：1963-08-01 00:00:00

abstract：:In vivo and in vitro methylation, methanol production assays, and the use of specific antibodies raised against the sensory transducing protein Tar in Escherichia coli all failed to demonstrate the presence of methyl-accepting chemotaxis proteins (MCPs) in the photosynthetic bacterium Rhodobacter sphaeroides, although...

journal_title：Journal of bacteriology

authors： Sockett RE,Armitage JP,Evans MC

更新日期：1987-12-01 00:00:00

abstract：:The O antigen is an important cell wall antigen of gram-negative bacteria, and the genes responsible for its biosynthesis are located in a gene cluster. We have cloned and sequenced the DNA segment unique to the O-antigen gene cluster of Salmonella enterica group D3. This segment includes a novel O-antigen polymerase ...

journal_title：Journal of bacteriology

authors： Curd H,Liu D,Reeves PR

更新日期：1998-02-01 00:00:00

abstract：:Emulsan, the polyanionic emulsifying agent synthesized by Acinetobacter calcoaceticus RAG-1, was depolymerized by an enzyme obtained from a soil bacterium YUV-1. The extracellular emulsan depolymerase was produced when strains RAG-1 and YUV-1 were grown together on agar medium. The enzyme was extracted from the agar a...

journal_title：Journal of bacteriology

authors： Shoham Y,Rosenberg E

更新日期：1983-10-01 00:00:00

abstract：:Orf20 of the conjugative transposon Tn916 was purified as a chimeric protein fused to maltose binding protein (MBP-Orf20). The chimeric protein possessed endonucleolytic activity, cleaving both strands of the Tn916 origin of conjugal transfer (oriT) at several distinct sites and favoring GT dinucleotides. Incubation o...

journal_title：Journal of bacteriology

authors： Rocco JM,Churchward G

更新日期：2006-03-01 00:00:00

abstract：:The terminal enzyme of heme biosynthesis, ferrochelatase (EC 4.99.1.1), catalyzes the insertion of ferrous iron into protoporphyrin IX to form protoheme. Prior to the present work, [2Fe-2S] clusters have been identified and characterized in animal ferrochelatases but not in plant or prokaryotic ferrochelatases. Herein...

journal_title：Journal of bacteriology

authors： Dailey TA,Dailey HA

更新日期：2002-05-01 00:00:00

abstract：:The direct binding of Streptococcus mitis to human platelets is mediated in part by two proteins (PblA and PblB) encoded by a lysogenic bacteriophage (SM1). Since SM1 is the first prophage of S. mitis that has been identified and because of the possible role of these phage-encoded proteins in virulence, we sought to c...

journal_title：Journal of bacteriology

authors： Siboo IR,Bensing BA,Sullam PM

更新日期：2003-12-01 00:00:00

abstract：:The β-barrel assembly machinery (BAM) complex is the core machinery for the assembly of β-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria. Discovery of integral membrane proteins that are key to pathogenesis and yet do not require assistance from the BAM complex raises the questi...

journal_title：Journal of bacteriology

authors： Hay ID,Belousoff MJ,Dunstan RA,Bamert RS,Lithgow T

更新日期：2018-02-07 00:00:00

abstract：:During colonization of the alfalfa rhizosphere, Pseudomonas fluorescens F113 undergoes phenotypic variation, resulting in the appearance of colonies with different morphology. Among phenotypic variants, three isolates, C, F, and S were selected, with the C variant showing colony morphology identical to that of the ino...

journal_title：Journal of bacteriology

authors： Sánchez-Contreras M,Martín M,Villacieros M,O'Gara F,Bonilla I,Rivilla R

更新日期：2002-03-01 00:00:00

abstract：:The global regulator CsrA (carbon storage regulator) is an RNA binding protein that coordinates central carbon metabolism, activates flagellum biosynthesis and motility, and represses biofilm formation in Escherichia coli. CsrA activity is antagonized by the untranslated RNA CsrB, to which it binds and forms a globula...

journal_title：Journal of bacteriology

authors： Suzuki K,Wang X,Weilbacher T,Pernestig AK,Melefors O,Georgellis D,Babitzke P,Romeo T

更新日期：2002-09-01 00:00:00

abstract：:Ruthenium red stained a capsule external to the exine of the Azotobacter cyst. The central body is therefore surrounded by three layers, the intine, the exine, and the capsule, all containing acid mucopolysaccharide. Vesicles that appear to originate from the contracting cell membrane of the central body may account f...

journal_title：Journal of bacteriology

authors： Pope LM,Wyss O

更新日期：1970-04-01 00:00:00

abstract：:In Streptococcus mutans, competence for genetic transformation and biofilm formation are dependent on the two-component signal transduction system ComDE together with the inducer peptide pheromone competence-stimulating peptide (CSP) (encoded by comC). Here, it is shown that the same system is also required for expres...

journal_title：Journal of bacteriology

authors： van der Ploeg JR

更新日期：2005-06-01 00:00:00

abstract：:We used Southern blotting to screen a variety of bacterial genes for homology to the kdp genes of Escherichia coli, genes that encode an ATP-driven K+ transport system. We found that most enterobacteria have sequences homologous to those of the three kdp structural genes and the kdpD regulatory gene. A number of dista...

journal_title：Journal of bacteriology

authors： Walderhaug MO,Litwack ED,Epstein W

更新日期：1989-02-01 00:00:00