Periplasmic structure of frozen-etched and negatively stained cells of Bacillus licheniformis as correlated with penicillinase formation.

abstract：:Transcription of an aqpZ-lac fusion in a single copy on the Escherichia coli chromosome increased as cells entered the stationary growth phase. This was true in a variety of media, and increased transcription in enriched medium required the RpoS sigma factor. Expression of the aqpZ-lac fusion was not affected by up- o...

journal_title：Journal of bacteriology

authors： Soupene E,King N,Lee H,Kustu S

更新日期：2002-08-01 00:00:00

abstract：UNLABELLED:Deacetylation of 7-aminocephalosporanic acid (7-ACA) at position C-3 provides valuable starting material for producing semisynthetic β-lactam antibiotics. However, few enzymes have been characterized in this process before now. Comparative analysis of the genome of the thermophilic bacterium Alicyclobacillus...

journal_title：Journal of bacteriology

authors： Ding JM,Yu TT,Han NY,Yu JL,Li JJ,Yang YJ,Tang XH,Xu B,Zhou JP,Tang HZ,Huang ZX

更新日期：2015-11-02 00:00:00

abstract：:Autoplaquing has been observed in Myxococcus strains freshly isolated from soil. Initial observations suggest that this phenomenon is not induced by elevated temperature or visible light; we suggest that it may be the result of a derangement in the developmental autolytic mechanism within the cell. ...

journal_title：Journal of bacteriology

authors： Breyen SA,Dworkin M

更新日期：1984-06-01 00:00:00

abstract：:The two 23S rRNA-5S rRNA-tRNAGly operons from the extreme thermophilic eubacterium Thermus thermophilus HB8 were used to characterized the in vivo processing and termination of 23S rRNA-5S rRNA-tRNAGly primary transcripts in this organism by nuclease S1 mapping. A processing site in the pre-23S rRNA 3'-flanking region...

journal_title：Journal of bacteriology

authors： Hartmann RK,Toschka HY,Erdmann VA

更新日期：1991-04-01 00:00:00

abstract：:Lee, Spencer H. S. (Dalhousie University, Halifax, Nova Scotia). Effect of 6-aminonicotinamide on chick-embryo tissue cultures infected with vaccinia virus. J. Bacteriol. 88:885-892. 1964.-6-Aminonicotinamide (6-AN) was shown to render chick-embryo fibroblast (CEF) tissue cultures refractory to infection with vaccinia...

journal_title：Journal of bacteriology

authors： LEE SH

更新日期：1964-10-01 00:00:00

abstract：:Two active lysine transport systems were detected in Saccharomycopsis lipolytica. No excretion of lysine out of the cells could be obtained, even by chasing with L-lysine or by poisoning with sodium azide. The kinetic properties of one of the permeases, the high-affinity lysine permease, were studied in detail. Its Km...

journal_title：Journal of bacteriology

authors： Beckerich JM,Heslot H

更新日期：1978-02-01 00:00:00

abstract：:The cysteine-rich envelope proteins of the elementary body form of chlamydiae are thought to be located in the outer membrane on the basis of their insolubility in the weak anionic detergent N-lauryl sarcosinate (Sarkosyl). We found, however, that the insolubility of the small (EnvA) and the large (EnvB) cysteine-rich...

journal_title：Journal of bacteriology

authors： Everett KD,Hatch TP

更新日期：1995-02-01 00:00:00

abstract：:The 5 to 10 peritrichously inserted complex flagella of Rhizobium meliloti MVII-1 were found to form right-handed flagellar bundles. Bacteria swam at speeds up to 60 microns/s, their random three-dimensional walk consisting of straight runs and quick directional changes (turns) without the vigorous angular motion (tum...

journal_title：Journal of bacteriology

authors： Götz R,Schmitt R

更新日期：1987-07-01 00:00:00

abstract：:Comprehensive analysis of genome-wide expression patterns during growth of the hyperthermophilic bacterium Thermotoga maritima on 14 monosaccharide and polysaccharide substrates was undertaken with the goal of proposing carbohydrate specificities for transport systems and putative transcriptional regulators. Saccharid...

journal_title：Journal of bacteriology

authors： Conners SB,Montero CI,Comfort DA,Shockley KR,Johnson MR,Chhabra SR,Kelly RM

更新日期：2005-11-01 00:00:00

abstract：:Adaptation in bacterial chemotaxis involves reversible methylation of specific glutamate residues within the cytoplasmic domains of methyl-accepting chemotaxis proteins. The specific sites of methylation in Salmonella enterica and Escherichia coli chemoreceptors, identified 2 decades ago, established a consensus seque...

journal_title：Journal of bacteriology

authors： Perez E,Zheng H,Stock AM

更新日期：2006-06-01 00:00:00

abstract：:The genome of Lactobacillus salivarius UCC118 includes a 242-kb megaplasmid, pMP118. We now show that 33 strains of L. salivarius isolated from humans and animals all harbor a megaplasmid, which hybridized with the repA and repE replication origin probes of pMP118. Linear megaplasmids that did not hybridize with the p...

journal_title：Journal of bacteriology

authors： Li Y,Canchaya C,Fang F,Raftis E,Ryan KA,van Pijkeren JP,van Sinderen D,O'Toole PW

更新日期：2007-09-01 00:00:00

abstract：:MobB is a small protein encoded by the broad-host-range plasmid R1162 and required for efficient mobilization of its DNA during conjugation. The protein was shown previously to stabilize the relaxosome, the complex of plasmid DNA and mobilization proteins at the origin of transfer (oriT). We have generated in-frame mo...

journal_title：Journal of bacteriology

authors： Perwez T,Meyer RJ

更新日期：1999-04-01 00:00:00

abstract：:We designed a selection strategy for the isolation of Escherichia coli mutants exhibiting enhanced protein disulfide isomerase activity. The folding of a variant of tissue plasminogen activator (v-tPA), a protein containing nine disulfide bonds, in the bacterial periplasm is completely dependent on the level of disulf...

journal_title：Journal of bacteriology

authors： Zhan X,Gao J,Jain C,Cieslewicz MJ,Swartz JR,Georgiou G

更新日期：2004-02-01 00:00:00

abstract：:Zorbamycin (U-30,604E) induces rapid degradation of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) in Bacillus subtilis cells. DNA degradation is initiated first and is closely followed by the degradation of RNA. No interaction between isolated DNA and zorbamycin is observed. Nucleic acid and protein syntheses...

journal_title：Journal of bacteriology

authors： Reusser F

更新日期：1971-10-01 00:00:00

abstract：:The effect of sublethal, midlethal, and lethal doses of total-body X irradiation on the fecal microbial population of the rat was studied. No changes were observed in animals receiving sublethal doses of X ray. Midlethal and lethal doses produced an increase in the numbers of fecal coliforms, staphylococci, streptococ...

journal_title：Journal of bacteriology

authors： Klainer AS,Gorbach S,Weinstein L

更新日期：1967-08-01 00:00:00

abstract：:We characterized the traU gene of the Escherichia coli K-12 conjugative plasmid F. Plasmids carrying segments of the F transfer operon were tested for their capacity to complement F lac traU526. The protein products of TraU+ clones were identified, and the nucleotide sequence of traU was determined. traU mapped betwee...

journal_title：Journal of bacteriology

authors： Moore D,Maneewannakul K,Maneewannakul S,Wu JH,Ippen-Ihler K,Bradley DE

更新日期：1990-08-01 00:00:00

abstract：:Forty years ago, Coulter and Talalay (A. W. Coulter and P. Talalay, J. Biol. Chem. 243:3238-3247, 1968) established the oxygenase-dependent pathway for the degradation of testosterone by aerobes. The oxic testosterone catabolic pathway involves several oxygen-dependent reactions and is not available for anaerobes. Sin...

journal_title：Journal of bacteriology

authors： Leu YL,Wang PH,Shiao MS,Ismail W,Chiang YR

更新日期：2011-09-01 00:00:00

abstract：:Bacteria of the genus Prosthecobacter express homologs of eukaryotic α- and β-tubulin, called BtubA and BtubB (BtubA/B), that have been observed to assemble into filaments in the presence of GTP. BtubA/B polymers are proposed to be composed in vitro by two to six protofilaments in contrast to that in vivo, where they ...

journal_title：Journal of bacteriology

authors： Díaz-Celis C,Risca VI,Hurtado F,Polka JK,Hansen SD,Maturana D,Lagos R,Mullins RD,Monasterio O

更新日期：2017-09-05 00:00:00

abstract：:Diploid cells of Saccharomyces cerevisiae underwent meiosis and sporulation when placed in 1% potassium acetate sporulation medium. In unbuffered sporulation medium the pH rose very rapidly, reaching pH 8.4 after 2 h of sporulation. Under these conditions, the uptake of radioactive adenine and lysine was extremely lim...

journal_title：Journal of bacteriology

authors： McCusker JH,Haber JE

更新日期：1977-10-01 00:00:00

abstract：:In a search for genes that produce hypersensitivity to cadmium salts in Escherichia coli, random transposon mutagenesis with TnphoA was used. One of the mutant strains obtained was sensitive to Cd2+ and Zn2+. Sequence analysis showed that the TnphoA insertion was located in the dsbA gene coding for a periplasmic prote...

journal_title：Journal of bacteriology

authors： Rensing C,Mitra B,Rosen BP

更新日期：1997-04-01 00:00:00

abstract：:The tar locus of Escherichia coli specifies one of the major species of methyl-accepting proteins involved in the chemotactic behavior of this organism. The physical and genetic organization of the tar region was investigated with a series of specialized lambda transducing phages and plasmid clones. The tar gene was m...

journal_title：Journal of bacteriology

authors： Slocum MK,Parkinson JS

更新日期：1983-08-01 00:00:00

abstract：:Acyl carrier protein was localized by immunoelectron microscopy in the cytoplasm of Escherichia coli. These data are inconsistent with the previous report of an association between acyl carrier protein and the inner membrane (H. Van den Bosch, J.R. Williamson, and P.R. Vagelos, Nature [London] 228:338-341, 1970). More...

journal_title：Journal of bacteriology

authors： Jackowski S,Edwards HH,Davis D,Rock CO

更新日期：1985-04-01 00:00:00

abstract：:The phoP-phoQ-regulated pagC locus is essential for full virulence and survival within macrophages of Salmonella typhimurium. The protein product, DNA sequence, and transcript of pagC were determined. The pagC locus encodes a single 188-amino-acid membrane protein that is similar to the ail-encoded eucaryotic cell inv...

journal_title：Journal of bacteriology

authors： Pulkkinen WS,Miller SI

更新日期：1991-01-01 00:00:00

abstract：:The GcvA protein is a LysR family regulatory protein necessary for both activation and repression of the Escherichia coli glycine cleavage enzyme operon (gcv) and negative regulation of gcvA. Gel shift assays indicated that overexpressed GcvA in crude extracts is capable of binding specifically to DNA containing the g...

journal_title：Journal of bacteriology

authors： Wilson RL,Urbanowski ML,Stauffer GV

更新日期：1995-09-01 00:00:00

abstract：:Sixty-four mutants were isolated from the LT-2 wild-type strain of Salmonella typhimurium by selecting for chromate resistance. The majority of lesions were shown to lie in the cysA gene. (i) The mutants cannot take up sulfate, a finding which verifies the role of cysA in sulfate transport. In addition, 52 sulfate-tra...

journal_title：Journal of bacteriology

authors： Ota N,Galsworthy PR,Pardee AB

更新日期：1971-03-01 00:00:00

abstract：:A temperate bacteriophage that determines the expression of enterohemolysin was isolated from Escherichia coli O26 strain C3888. The genetic determinant associated with enterohemolysin production (E-Hly determinant) was cloned from EcoRI-digested bacteriophage DNA in vector plasmid pUC8. pUC8 recombinant plasmid pEO19...

journal_title：Journal of bacteriology

authors： Beutin L,Bode L,Ozel M,Stephan R

更新日期：1990-11-01 00:00:00

abstract：:Specific localization of the lysis (L) protein of bacteriophage MS2 in the cell wall of Escherichia coli was determined by immunoelectron microscopy. After induction of the cloned lysis gene, the cells were plasmolyzed, fixed, and embedded in either Epon or Lowicryl K4M. Polyclonal L-protein-specific antiserum was pur...

journal_title：Journal of bacteriology

authors： Walderich B,Höltje JV

更新日期：1989-06-01 00:00:00

abstract：:Smith, Paul F. (University of Pennsylvania, Philadelphia) and George H. Rothblat. Comparison of pleuropneumonia-like and L-type organisms. J. Bacteriol. 83:500-506. 1962.-The content of total lipid and nonsaponifiable lipid depended upon the type of organism; the greatest amounts were found in pleuropneumonia-like org...

journal_title：Journal of bacteriology

authors： SMITH PF,ROTHBLAT GH

更新日期：1962-03-01 00:00:00

abstract：:Transposition is a DNA reorganization reaction potentially deleterious for the host. The frequency of transposition is limited by the amount of transposase. Therefore, strict regulation of a transposase is required to keep control over the destructive multiplication of the mobile element. We have shown previously that...

journal_title：Journal of bacteriology

authors： Hõrak R,Kivisaar M

更新日期：1999-10-01 00:00:00

abstract：:To investigate the function of spot 42 RNA, a small RNA of Escherichia coli, we constructed a strain in which spf, the structural gene for this RNA, is deleted. We achieved this by using a delta att phage lambda carrying a DNA fragment spanning the spf region but with a precise deletion of spf. By integration of this ...

journal_title：Journal of bacteriology

authors： Hatfull GF,Joyce CM

更新日期：1986-06-01 00:00:00