Defined medium for growth of Haemophilus influenzae.

abstract：:Neisseria meningitidis expresses multicomponent organelles called type four pili (Tfp), which are key virulence factors required for attachment to human cells during carriage and disease. Pilin (PilE) is the main component of Tfp, and N. meningitidis isolates either have a class I pilE locus and express pilins that un...

journal_title：Journal of bacteriology

authors： Lobanovska M,Tang CM,Exley RM

更新日期：2019-09-20 00:00:00

abstract：:The structural gene for the F sex factor outer membrane surface exclusion protein ( traT ) was cloned onto a high-copy-number plasmid where it is expressed from the phage lambda promoter pL. Conditional control over expression was provided by a temperature-sensitive lambda cI repressor. Induction of pL produced large ...

journal_title：Journal of bacteriology

authors： Minkley EG Jr

更新日期：1984-05-01 00:00:00

abstract：:A novel iron(III) transport system of Serratia marcescens, named SFU, was cloned and characterized in Escherichia coli. Iron acquisition by this system differed from that by E. coli and related organisms. No siderophore production and no receptor protein related to the SFU system could be detected. In addition, iron u...

journal_title：Journal of bacteriology

authors： Zimmermann L,Angerer A,Braun V

更新日期：1989-01-01 00:00:00

abstract：:Cysteine and methionine availability influences many processes in the cell. In bacteria, transcription of the specific genes involved in the synthesis of these two amino acids is usually regulated by different mechanisms or regulators. Pathways for the synthesis of cysteine and methionine and their interconversion wer...

journal_title：Journal of bacteriology

authors： Sperandio B,Polard P,Ehrlich DS,Renault P,Guédon E

更新日期：2005-06-01 00:00:00

abstract：:A lambda phage (lambdaNK55) carrying the translocatable element Tn10, conferring tetracycline resistance (Tetr), has been utilized to isolate glutamine auxotrophs of Escherichia coli K-12. Such strains lack uridylyltransferase as a result of an insertion of the TN10 element in the glnD gene. The glnD::Tn10 insertion h...

journal_title：Journal of bacteriology

authors： Bloom FR,Levin MS,Foor F,Tyler B

更新日期：1978-05-01 00:00:00

abstract：:Two replicons were isolated independently from different IncHI1 plasmids. One was isolated from R27, and a second was isolated from pIP522. We demonstrate, by DNA-DNA hybridization experiments, that these maintenance regions are different and that they are specific to, and carried by, all IncHI1 plasmids tested. In vi...

journal_title：Journal of bacteriology

authors： Gabant P,Newnham P,Taylor D,Couturier M

更新日期：1993-12-01 00:00:00

abstract：:Two isoleucine analogues and two leucine analogues were examined for their ability to replace the natural amino acid preventing the accumulation of threonine deaminase-forming potential. The procedure used to study repression by the analogues distinguishes between true repression and the formation of inactive enzyme b...

journal_title：Journal of bacteriology

authors： Wasmuth JJ,Umbarger HE

更新日期：1973-11-01 00:00:00

abstract：:The impact of arsenite [As(III)] on several levels of cellular metabolism and gene regulation was examined in Pseudomonas aeruginosa. P. aeruginosa isogenic mutants devoid of antioxidant enzymes or defective in various metabolic pathways, DNA repair systems, metal storage proteins, global regulators, or quorum sensing...

journal_title：Journal of bacteriology

authors： Parvatiyar K,Alsabbagh EM,Ochsner UA,Stegemeyer MA,Smulian AG,Hwang SH,Jackson CR,McDermott TR,Hassett DJ

更新日期：2005-07-01 00:00:00

abstract：:soxR governs a superoxide response regulon that contains the genes for endonuclease IV, Mn2(+)-superoxide dismutase, and glucose 6-phosphate dehydrogenase. The soxR gene encodes a 17-kDa protein; some mutations of this gene cause constitutive overexpression of the regulon. Induction by paraquat (methyl viologen) requi...

journal_title：Journal of bacteriology

authors： Wu J,Weiss B

更新日期：1991-05-01 00:00:00

abstract：:Phosphoenolypyruvate-dependent maltose:phosphotransferase activity was induced in cells of Fusobacterium mortiferum ATCC 25557 during growth on maltose. The disaccharide was rapidly metabolized by washed cells maintained under anaerobic conditions, but fermentation ceased immediately upon exposure of the cell suspensi...

journal_title：Journal of bacteriology

authors： Robrish SA,Fales HM,Gentry-Weeks C,Thompson J

更新日期：1994-06-01 00:00:00

abstract：:The murG gene of Escherichia coli encodes the N-acetylglucosaminyltransferase responsible for the final step in the formation of the lipid-linked disaccharide-pentapeptide subunit of peptidoglycan. Using trypsin to probe maxicell spheroplasts, we show that this enzyme is peripherally associated with the inner face of ...

journal_title：Journal of bacteriology

authors： Bupp K,van Heijenoort J

更新日期：1993-03-01 00:00:00

abstract：:Analysis of a region involved in the conjugative transfer of the lactococcal conjugative element pRS01 has revealed a bacteria] group II intron. Splicing of this lactococcal intron (designated Ll.ltrB) in vivo resulted in the ligation of two exon messages (ltrBE1 and ltrBE2) which encoded a putative conjugative relaxa...

journal_title：Journal of bacteriology

authors： Mills DA,McKay LL,Dunny GM

更新日期：1996-06-01 00:00:00

abstract：:Peptide deformylase (PDF) is an essential bacterial metalloprotease involved in deformylation of N-formyl group from nascent polypeptide chains during protein synthesis. Iron-containing variants of this enzyme from Salmonella enterica serovar Typhimurium (sPDF) and Mycobacterium tuberculosis (mPDF), although inhibited...

journal_title：Journal of bacteriology

authors： Kumar S,Kanudia P,Karthikeyan S,Chakraborti PK

更新日期：2014-01-01 00:00:00

abstract：:Microbial communities span many orders of magnitude, ranging in scale from hundreds of cells on a single particle of soil to billions of cells within the lumen of the gastrointestinal tract. Bacterial cells in all habitats are members of densely populated local environments that facilitate competition between neighbor...

journal_title：Journal of bacteriology

authors： Stubbendieck RM,Straight PD

更新日期：2016-07-28 00:00:00

abstract：:An m(k) (-)r(k) (-) mutation exerted no measurable effect on 5-bromouracil incorporation by a thymineless derivative of Escherichia coli K. ...

journal_title：Journal of bacteriology

authors： Matney TS,MacDougall NL,Butler MA,Suit JC

更新日期：1970-10-01 00:00:00

abstract：:The highly defective rho-15 mutant of Escherichia coli produces plasmid DNA that is 22% less negatively supercoiled than DNA from an isogenic wild-type strain (J. S. Fassler, G. F. Arnold, and I. Tessman, Mol. Gen. Genet. 204:424-429, 1986). We extended our measurements of plasmid superhelicity to additional rho mutan...

journal_title：Journal of bacteriology

authors： Arnold GF,Tessman I

更新日期：1988-09-01 00:00:00

abstract：:Brucella native haptens (NHs) extracted with hot water from smooth (S)-type B. abortus and B. melitensis were purified to high levels of serological activity and compared with the polysaccharide obtained by acid hydrolysis (PS) of the S lipopolysaccharide (S-LPS). By 13C nuclear magnetic resonance analysis, NHs showed...

journal_title：Journal of bacteriology

authors： Aragón V,Díaz R,Moreno E,Moriyón I

更新日期：1996-02-01 00:00:00

abstract：:The origin of replication of Bacillus subtilis 168 trp thy dna-1 (temperature-sensitive initiation mutant) was labeled with [3H]thymidine. Analysis of labeled cells by autoradiography revealed that most of the radioactivity was associated with cell pole areas. To label the terminus, cells that had initiated were treat...

journal_title：Journal of bacteriology

authors： Sonnenfeld EM,Koch AL,Doyle RJ

更新日期：1985-09-01 00:00:00

abstract：:We constructed mercury resistance operon-luciferase (mer-lux) transcriptional fusion plasmids to evaluate in vivo gene expression rates of the mer structural gene promoter (PTPCAD) of transposon Tn21. In vivo gene expression kinetics corresponded well with those previously determined in vitro, yielding an apparent K0....

journal_title：Journal of bacteriology

authors： Condee CW,Summers AO

更新日期：1992-12-01 00:00:00

abstract：:From several presumably pure cultures of Thiobacillus ferrooxidans, we isolated a pair of stable phenotypes. One was a strict autotroph utilizing sulfur or ferrous iron as the energy source and unable to utilize glucose; the other phenotype was an acidophilic obligate heterotroph capable of utilizing glucose but not s...

journal_title：Journal of bacteriology

authors： Harrison AP Jr,Jarvis BW,Johnson JL

更新日期：1980-07-01 00:00:00

abstract：:Raps, Shirley (University of Illinois, Urbana) and R. D. DeMoss. Glycolytic enzymes in Zymomonas mobilis. J. Bacteriol. 84:115-118. 1962-An enzyme extract of Zymomonas mobilis (Pseudomonas lindneri) was capable of fermenting glucose-6-phosphate to CO(2) and ethanol. The extract was found to contain phosphohexoisomeras...

journal_title：Journal of bacteriology

authors： RAPS S,DEMOSS RD

更新日期：1962-07-01 00:00:00

abstract：:Alcaligenes eutrophus H16 produces two [NiFe] hydrogenases which catalyze the oxidation of hydrogen and enable the organism to utilize H2 as the sole energy source. The genes (hoxK and hoxG) for the heterodimeric, membrane-bound hydrogenase (MBH) are located adjacent to a series of eight accessory genes (hoxZ, hoxM, h...

journal_title：Journal of bacteriology

authors： Bernhard M,Schwartz E,Rietdorf J,Friedrich B

更新日期：1996-08-01 00:00:00

abstract：:The Arc (anoxic redox control) two-component signal transduction system, consisting of the ArcB sensor kinase and the ArcA response regulator, allows adaptive responses of Escherichia coli to changes of O(2) availability. The arcA gene was previously known as the dye gene because null mutants were growth sensitive to ...

journal_title：Journal of bacteriology

authors： Alvarez AF,Malpica R,Contreras M,Escamilla E,Georgellis D

更新日期：2010-01-01 00:00:00

abstract：:The location and extent of the homology between plasmids ColE1 and p15A were determined by analysis of heteroduplexes formed between them as well as with a related plasmid, pBR322, and by hybridization of radioactive deoxyribonucleic acids to restriction fragments of p15A and ColE1. The homology between the plasmids c...

journal_title：Journal of bacteriology

authors： Bird RE

更新日期：1981-03-01 00:00:00

abstract：:Germination and outgrowth of ascospores of Saccharomyces cerevisiae 4579 require both methionine and adenine, whereas leucine is only required for outgrowth. The methionine requirement may be satisfied by S-adenosylmethionine, but this sulfonium compound will not substitute for adenine. Between 30 and 70 min of protei...

journal_title：Journal of bacteriology

authors： Choih SJ,Ferro AJ,Shapiro SK

更新日期：1977-07-01 00:00:00

abstract：:The Escherichia coli nusA gene, nusAEc, encodes an essential protein that influences transcription elongation. Derivatives of E. coli in which the Salmonella typhimurium nusA gene, nusASt, has replaced nusAEc are viable. Thus, NusASt can substitute for NusAEc in supporting essential bacterial activities. However, hybr...

journal_title：Journal of bacteriology

authors： Craven MG,Granston AE,Schauer AT,Zheng C,Gray TA,Friedman DI

更新日期：1994-03-01 00:00:00

abstract：:We have sequenced the entire region of DNA required for the biosynthesis of CS5 pili from enterotoxigenic Escherichia coli O115:H40 downstream of the major subunit gene, designated csfA (for coli surface factor five A). Five more open reading frames (ORFs) (csfB, csfC, csfE, csfF, and csfD) which are transcribed in th...

journal_title：Journal of bacteriology

authors： Duthy TG,Staendner LH,Manning PA,Heuzenroeder MW

更新日期：1999-09-01 00:00:00

abstract：:The colH gene encoding a collagenase was cloned from Clostridium histolyticum JCM 1403. Nucleotide sequencing showed a major open reading frame encoding a 116-kDa protein of 1,021 amino acid residues. The deduced amino acid sequence contains a putative signal sequence and a zinc metalloprotease consensus sequence, HEX...

journal_title：Journal of bacteriology

authors： Yoshihara K,Matsushita O,Minami J,Okabe A

更新日期：1994-11-01 00:00:00

abstract：:The Escherichia coli beta-galactosidase enzyme was used as a reporter molecule for genetic fusions in Rhodobacter capsulatus. DNA fragments that were from the upstream region of the hydrogenase structural operon hupSLM and contained 5' hupS sequences were fused in frame to a promoterless lacZ gene, yielding fusion pro...

journal_title：Journal of bacteriology

authors： Colbeau A,Vignais PM

更新日期：1992-07-01 00:00:00

abstract：:Carbon catabolite repression of maltase gene expression is brought about by the addition of glucose, resulting in a drastic inhibition of the induction of maltase. When added to induced cells, glucose leads to the inhibition of maltase synthesis within 30 min, which can be accounted for by the disappearance of hybridi...

journal_title：Journal of bacteriology

authors： Federoff HJ,Eccleshall TR,Marmur J

更新日期：1983-10-01 00:00:00