L-cysteine biosynthesis in Bacillus subtilis: identification, sequencing, and functional characterization of the gene coding for phosphoadenylylsulfate sulfotransferase.

Abstract:

:Random Tn917 mutagenesis of Bacillus subtilis followed by selection of lipoic acid auxotrophs led to the isolation of the cysH gene. The gene was sequenced and found to encode a phosphoadenylylsulfate sulfotransferase with a molecular mass of 27 kDa. Expression of lacZ fused to the cysH promoter was repressed by cysteine and sulfide and induced by sulfur limitation, indicating that cysH is controlled at the level of transcription.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Mansilla MC,de Mendoza D

doi

10.1128/jb.179.3.976-981.1997

subject

Has Abstract

pub_date

1997-02-01 00:00:00

pages

976-81

issue

3

eissn

0021-9193

issn

1098-5530

journal_volume

179

pub_type

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