Abstract:
:The tsr gene specifies a methyl-accepting membrane protein involved in chemotaxis to serine and several repellent compounds. We have characterized a special class of tsr mutations designated cheD which alter the signaling properties of the Tsr transducer. Unlike tsr null mutants, cheD strains are generally nonchemotactic, dominant in complementation tests, and exhibit a pronounced counterclockwise bias in flagellar rotation. Several lines of evidence showed that cheD mutations were alleles of the tsr gene. First, cheD mutations were mapped into the same deletion segments as conventional tsr mutations. Second, restriction site analysis of the transducing phage deletions used to construct the genetic map demonstrated that the endpoints of the deletion segments fell within the tsr coding sequence. Third, a number of the cheD mutants synthesized Tsr proteins with slight changes in electrophoretic mobility, consistent with alterations in Tsr primary structure. These mutant proteins were able to undergo posttranslational deamidation and methylation reactions in the same manner as wild-type Tsr protein; however, the steady-state level of Tsr methylation in cheD strains was very high. The methylation state of the Tar protein, another species of methyl-accepting protein in Escherichia coli, was also higher than normal in cheD strains, suggesting that the aberrant Tsr transducer in cheD mutants has a generalized effect on the sensory adaptation system of the cell. These properties are consistent with the notion that the Tsr protein of cheD mutants is locked in an excitatory signaling mode that both activates the sensory adaptation system and drowns out chemotactic signals generated by other transducer species. Further study of cheD mutations thus promises to reveal valuable information about the functional architecture of the Tsr protein and how this transducer controls flagellar behavior.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Callahan AM,Parkinson JSdoi
10.1128/JB.161.1.96-104.1985subject
Has Abstractpub_date
1985-01-01 00:00:00pages
96-104issue
1eissn
0021-9193issn
1098-5530journal_volume
161pub_type
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.94.5.1300-1305.1967
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pub_type: 杂志文章
doi:10.1128/JB.92.1.107-115.1966
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pub_type: 杂志文章
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更新日期:1990-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:2012-11-01 00:00:00
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pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
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pub_type: 杂志文章
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更新日期:2001-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.2.702-710.2006
更新日期:2006-01-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.9.2599-2606.1993
更新日期:1993-05-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.178.18.5417-5421.1996
更新日期:1996-09-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.136.2.815-817.1978
更新日期:1978-11-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.3.1151-1157.1979
更新日期:1979-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/JB.157.3.899-908.1984
更新日期:1984-03-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/jb.175.18.5945-5952.1993
更新日期:1993-09-01 00:00:00
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更新日期:1970-10-01 00:00:00
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更新日期:1986-07-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.2.343-354.1965
更新日期:1965-02-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.3.946-954.1976
更新日期:1976-03-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00459-07
更新日期:2007-08-01 00:00:00
abstract::Shah, D. B. (University of Wisconsin, Madison), and J. B. Wilson. Egg yolk factor of Staphylococcus aureus. II. Characterization of the lipase activity. J. Bacteriol. 89:949-953. 1965.-The staphylococcal egg yolk factor was characterized as a lipase. The enzyme had an optimal pH of 7.8, but the optimal pH of stability...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.4.949-953.1965
更新日期:1965-04-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.9.5187-5189.1989
更新日期:1989-09-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2432-2439.1987
更新日期:1987-06-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01050-13
更新日期:2014-01-01 00:00:00
abstract:UNLABELLED:The transmembrane Tsr protein of Escherichia coli mediates chemotactic responses to environmental serine gradients. Serine binds to the periplasmic domain of the homodimeric Tsr molecule, promoting a small inward displacement of one transmembrane helix (TM2). TM2 piston displacements, in turn, modulate the s...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00274-15
更新日期:2015-08-01 00:00:00
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00622-08
更新日期:2008-09-01 00:00:00
abstract::Salmonella typhimurium and Escherichia coli from anaerobic cultures displayed tactic responses to gradients of nitrate, fumarate, and oxygen when the appropriate electron transport pathway was present. Such responses were named "electron acceptor taxis" because they are elicited by terminal electron acceptors. Mutant ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.140.2.567-573.1979
更新日期:1979-11-01 00:00:00