Abstract:
:The serotype 5 capsule gene cluster of Staphylococcus aureus comprises 16 genes (cap5A through cap5P), but little is known about how the putative gene products function in capsule biosynthesis. We propose that the N-acetylmannosaminuronic acid (ManNAcA) component of the S. aureus serotype 5 capsular polysaccharide (CP5) is synthesized from a UDP-N-acetylglucosamine (UDP-GlcNAc) precursor that is epimerized to UDP-N-acetylmannosamine (UDP-ManNAc) and then oxidized to UDP-ManNAcA. We report the purification and biochemical characterization of a recombinant UDP-GlcNAc 2-epimerase encoded by S. aureus cap5P. Purified Cap5P converted approximately 10% of UDP-GlcNAc to UDP-ManNAc as detected by gas chromatography-mass spectrometry. The epimerization of UDP-GlcNAc to UDP-ManNAc occurred over a wide pH range and was unaffected by divalent cations. Surprisingly, CP5 expression in S. aureus was unaffected by insertional inactivation of cap5P. Sequence homology searches of the public S. aureus genomic databases revealed the presence of another putative UDP-GlcNAc 2-epimerase on the S. aureus chromosome that showed 61% identity to Cap5P. Redundancy of UDP-GlcNAc 2-epimerase function in S. aureus was demonstrated by cloning the cap5P homologue from strain Newman and complementing an Escherichia coli rffE mutant defective in UDP-GlcNAc 2-epimerase activity. Our results confirm the putative function of the S. aureus cap5P gene product and demonstrate the presence of a second gene on the staphylococcal chromosome with a similar function.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Kiser KB,Bhasin N,Deng L,Lee JCdoi
10.1128/JB.181.16.4818-4824.1999keywords:
subject
Has Abstractpub_date
1999-08-01 00:00:00pages
4818-24issue
16eissn
0021-9193issn
1098-5530journal_volume
181pub_type
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journal_title:Journal of bacteriology
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pub_type: 杂志文章
doi:10.1128/JB.145.2.1085-1090.1981
更新日期:1981-02-01 00:00:00
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journal_title:Journal of bacteriology
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doi:10.1128/JB.124.2.843-848.1975
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journal_title:Journal of bacteriology
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更新日期:1970-08-01 00:00:00
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
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journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.7.3830-3836.1990
更新日期:1990-07-01 00:00:00
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更新日期:2002-03-01 00:00:00