Autoradiographic analysis of diaminopimelic acid incorporation in filamentous cells of Escherichia coli: repression of peptidoglycan synthesis around the nucleoid.

Abstract:

:Peptidoglycan synthesis rate in nonconstricting filaments of Escherichia coli dnaX(Ts) has been studied by autoradiography of incorporated [3H]diaminopimelic acid. Analysis of autoradiograms of whole cells and sacculi showed that peptidoglycan is synthesized at a reduced rate in the nucleoid-containing parts of these filaments. The lower rate of peptidoglycan synthesis in the cell center coincides with a higher local rate of protein synthesis. DNA-less cell formation in dnaX(Ts), dnaX(Ts) sfiA, and the minB minicell-forming mutant is accompanied by a local increase in peptidoglycan synthesis at the constriction site.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Mulder E,Woldringh CL

doi

10.1128/jb.173.15.4751-4756.1991

subject

Has Abstract

pub_date

1991-08-01 00:00:00

pages

4751-6

issue

15

eissn

0021-9193

issn

1098-5530

journal_volume

173

pub_type

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