Abstract:
:Pseudomonas stutzeri A1501 is a versatile nitrogen-fixing bacterium capable of living in diverse environments and coping with various oxidative stresses. NfiS, a regulatory noncoding RNA (ncRNA) involved in the control of nitrogen fixation in A1501, was previously shown to be required for optimal resistance to H2O2; however, the precise role of NfiS and the target genes involved in the oxidative stress response is entirely unknown. In this work, we systematically investigated the NfiS-based mechanisms underlying the response of this bacterium to H2O2 at the cellular and molecular levels. A mutant strain carrying a deletion of nfiS showed significant downregulation of oxidative stress response genes, especially katB, a catalase gene, and oxyR, an essential regulator for transcription of catalase genes. Secondary structure prediction revealed two binding sites in NfiS for katB mRNA. Complementation experiments using truncated nfiS genes showed that each of two sites is functional, but not sufficient, for NfiS-mediated regulation of oxidative stress resistance and nitrogenase activities. Microscale thermophoresis assays further indicated direct base pairing between katB mRNA and NfiS at both sites 1 and 2, thus enhancing the half-life of the transcript. We also demonstrated that katB expression is dependent on OxyR and that both OxyR and KatB are essential for optimal oxidative stress resistance and nitrogenase activities. H2O2 at low concentrations was detoxified by KatB, leaving O2 as a by-product to support nitrogen fixation under O2-insufficient conditions. Moreover, our data suggest that the direct interaction between NfiS and katB mRNA is a conserved and widespread mechanism among P. stutzeri strains.IMPORTANCE Protection against oxygen damage is crucial for survival of nitrogen-fixing bacteria due to the extreme oxygen sensitivity of nitrogenase. This work exemplifies how the small ncRNA NfiS coordinates oxidative stress response and nitrogen fixation via base pairing with katB mRNA and nifK mRNA. Hence, NfiS acts as a molecular link to coordinate the expression of genes involved in oxidative stress response and nitrogen fixation. Our study provides the first insight into the biological functions of NfiS in oxidative stress regulation and adds a new regulation level to the mechanisms that contribute to the oxygen protection of the MoFe nitrogenase.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Zhang H,Zhan Y,Yan Y,Liu Y,Hu G,Wang S,Yang H,Qiu X,Liu Y,Li J,Lu W,Elmerich C,Lin Mdoi
10.1128/JB.00334-19subject
Has Abstractpub_date
2019-09-06 00:00:00issue
19eissn
0021-9193issn
1098-5530pii
JB.00334-19journal_volume
201pub_type
杂志文章abstract::Margherita, S. S. (Temple University School of Medicine, Philadelphia, Pa.), and H. Friedmann. Induction of nonspecific resistance by endotoxin in unresponsive mice. J. Bacteriol. 89:277-280. 1965.-Serratia marcescens endotoxin was tested for protective effect in animals of varying immunological competence. A quantity...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.2.277-280.1965
更新日期:1965-02-01 00:00:00
abstract::Mutational analysis of the carboxy-terminal region of Escherichia coli HlyC was performed by site-directed mutagenesis. Replacement of residue Val-127 or Lys-129 reduced the activity of HlyC to about 30 or 60%, respectively, of that of the wild type, while replacement of Gly-128 reduced the activity to less than 1% of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.18.5959-5962.1997
更新日期:1997-09-01 00:00:00
abstract::The sugar composition of lipopolysaccharide (LPS) isolated from whole cells of Alteromonas haloplanktis 214 (previously referred to as marine pseudomonas B-16, ATCC 19855), variant 3, of the lipid A, core, and side-chain fractions derived from it, and of the LPS fractions (LPS I, II, and III) obtained by subjecting it...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.1.158-167.1978
更新日期:1978-10-01 00:00:00
abstract::Procedures for the purification of catechol 1,2-dioxygenase from extracts of Acinetobacter calcoaceticus strain ADP-96 are described. The purified enzyme was homogeneous as judged by ultracentrifugation and acrylamide gel electrophoresis. The enzyme contained 2 g-atoms of iron per mol of protein. The enzyme had a broa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.127.1.536-544.1976
更新日期:1976-07-01 00:00:00
abstract::A denitrifying Pseudomonas sp. is able to oxidize aromatic compounds compounds completely to CO2, both aerobically and anaerobically. It is shown that benzoate is aerobically oxidized by a new degradation pathway via benzoyl-coenzyme A (CoA) and 3-hydroxybenzoyl-CoA. The organism grew aerobically with benzoate, 3-hydr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.15.4851-4858.1993
更新日期:1993-08-01 00:00:00
abstract::Entry into intestinal epithelial cells is an essential step in the pathogenesis of Salmonella infections. Our laboratory has previously identified a genetic locus, inv, that is necessary for efficient entry of Salmonella typhimurium into cultured epithelial cells. We have carried out a molecular and functional analysi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.15.4501-4510.1994
更新日期:1994-08-01 00:00:00
abstract::The presence of plasmid gene cadB did not affect Cd2+ accumulation, whereas plasmid gene cadA reduced Cd2+ accumulation by whole cells but not by membrane vesicles. Membrane vesicle studies indicated that Cd2+ uptake occurred via the Mn2+ transport system which was energized by the membrane electrical potential. Mn2+ ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.150.2.973-976.1982
更新日期:1982-05-01 00:00:00
abstract::A mutant affected in the b subunit (coded by the uncF gene) of the F1F0-ATPase in Escherichia coli was isolated by a localized mutagenesis procedure in which a plasmid carrying the unc genes was mutagenized in vivo. The biochemical properties of cells carrying the uncF515 allele were examined in a strain carrying the ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.162.1.420-426.1985
更新日期:1985-04-01 00:00:00
abstract::Streptococcus thermophilus strain CNRZ 302 is unable to ferment galactose, neither that generated intracellularly by lactose hydrolysis nor the free sugar. Nevertheless, sequence analysis and complementation studies with Escherichia coli demonstrated that strain CNRZ 302 contained structurally intact genes for the Lel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.4.1184-1194.2001
更新日期:2001-02-01 00:00:00
abstract::We have cloned a lytic enzyme, PlyPH, with a specific lytic effect on Bacillus anthracis strains. PlyPH remains active between pH 4 and 10.5, and a single dose rescued a significant percentage of mice infected intraperitoneally with an attenuated B. anthracis strain. We propose PlyPH as a novel therapeutic agent. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.188.7.2711-2714.2006
更新日期:2006-04-01 00:00:00
abstract::The effects of nickel on the expression of hydrogenase in the hydrogen-oxidizing bacterium Alcaligenes latus were studied. In the absence of added nickel, both hydrogenase activity, measured as O2-dependent H2 uptake, and hydrogenase protein, measured in a Western immunoblot, were very low compared with the levels in ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.9.3891-3896.1988
更新日期:1988-09-01 00:00:00
abstract::Salmonella virulence requires the initial invasion of host cells, followed by modulation of the intracellular environment for survival and replication. In an effort to characterize the role of small RNAs in Salmonella pathogenesis, we inadvertently identified a 5-kDa protein named YshB that is involved in the intracel...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00314-19
更新日期:2019-08-08 00:00:00
abstract::Although yeast cannot normally incorporate exogenous deoxythymidine 5'-monophosphate (dTMP) into deoxyribonucleic acid, mutants able to do so have been isolated. We have characterized a recessive suppressor of dTMP uptake (sot1) that prevents strains carrying either tup1, tup2, or tup4 from growing on selective medium...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.138.2.638-641.1979
更新日期:1979-05-01 00:00:00
abstract::Amino acid transport rates and amino acid binding proteins were examined in a strain containing the rho-120 mutation (formerly SuA), which has been shown to lower the rho-dependent, ribonucleic acid-activated adenosine triphosphatase activity to 9% of the rho activity in the isogenic wild-type strain. Tryptophan and p...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.130.3.1024-1029.1977
更新日期:1977-06-01 00:00:00
abstract::CTXphi is a filamentous bacteriophage that encodes cholera toxin. CTXphi infection of its host bacterium, Vibrio cholerae, requires the toxin-coregulated pilus (TCP) and the products of the V. cholerae tolQRA genes. Here, we have explored the role of OrfU, a predicted CTXphi minor coat protein, in CTXphi infection. Pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.3.1037-1044.2003
更新日期:2003-02-01 00:00:00
abstract::A genetic approach was used to establish the route of UMP biosynthesis in Sulfolobus acidocaldarius, a member of the hyperthermophilic division (the Crenarchaeota) of the Archaea domain. Pyrimidine auxotrophs of S. acidocaldarius DG6 were isolated by direct selection and by brute-force methods. Enzymatic assay of extr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.5.1500-1507.1993
更新日期:1993-03-01 00:00:00
abstract::The cysB region in Salmonella typhimurium regulates in a positive manner the noncontiguous structural genes for the enzymes responsible for sulfate reduction in cysteine biosynthesis. We treated three cysB mutants with chemical mutagens and selected 81 secondary mutants in which the inability to utilize sulfate was su...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.125.1.94-101.1976
更新日期:1976-01-01 00:00:00
abstract::Loci termed rfa, determining biosynthesis of somatic lipopolysaccharide core, have been mapped in Salmonella typhimurium LT2. The smooth-specific phage P22 co-transduced two leaky rfa alleles with cysE and with pyrE; one of the leaky alleles is perhaps rfaG, and the other is an unidentified gene concerned with synthes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.112.1.48-57.1972
更新日期:1972-10-01 00:00:00
abstract::In stationary phase, 95% of the fructosyltransferase (FTase) activity of Streptococcus salivarius ATCC 25975 was found associated with the cells. Within the first 15 min after inoculation into fresh medium, the specific activity of cell-associated FTase decreased by 92% of its initial value. After this period of initi...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.3.912-918.1981
更新日期:1981-12-01 00:00:00
abstract::Intracellular serine proteases of Bacillus licheniformis and Bacillus subtilis are closely related. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.137.2.1017-1019.1979
更新日期:1979-02-01 00:00:00
abstract::A mutant, cyaR1, affecting regulation of adenylate cyclase expression or activity is described. It was obtained as a thermoresistant revertant of a strain harboring a thermosensitive transcription termination factor, rho (rho-15). This mutant failed to synthesize adenosine 3',5'-phosphate and exhibited a carbohydrate-...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.148.3.753-761.1981
更新日期:1981-12-01 00:00:00
abstract::The plasmid nature of genes specifying degradation of nicotine and nicotinate in Pseudomas convexa strain 1 (Pc1) is indicated by mitomycin curing and conjugational transfer to other strains. The NIC plasmid appears to be compatible with other metabolic plasmids in Pseudomonas putida. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.135.1.289-290.1978
更新日期:1978-07-01 00:00:00
abstract::Diploid cells of Saccharomyces cerevisiae underwent meiosis and sporulation when placed in 1% potassium acetate sporulation medium. In unbuffered sporulation medium the pH rose very rapidly, reaching pH 8.4 after 2 h of sporulation. Under these conditions, the uptake of radioactive adenine and lysine was extremely lim...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.132.1.180-185.1977
更新日期:1977-10-01 00:00:00
abstract::Total DNA of various Rhizobium galegae strains representing different geographical origins, and taxonomic divergence was digested with three restriction enzymes separately, Southern blotted, and hybridized with six heterologous probes. The sequence divergences for different pairwise comparisons were calculated from pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.10.5561-5566.1989
更新日期:1989-10-01 00:00:00
abstract::The location of the Escherichia coli K-12 genes determining or regulating glutamate transport, and the location of the gene determining glutamate decarboxylase synthesis, were established by conjugation. The ability to grow on glutamate as the sole source of carbon and energy was used to select for glutamate transport...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.93.4.1409-1415.1967
更新日期:1967-04-01 00:00:00
abstract::Resistance to multiple antibiotics and certain oxidative stress compounds was conferred by three independently selected mutations (marR1, soxQ1, and cfxB1) that mapped to 34 min on the Escherichia coli chromosome. Mutations at this locus can activate the marRAB operon, in which marR encodes a putative repressor of mar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.1.143-148.1994
更新日期:1994-01-01 00:00:00
abstract::Gilden, R. V. (Wistar Institute, Philadelphia, Pa.), and R. I. Carp. Effects of cycloheximide and puromycin on synthesis of simian virus 40 T antigen in green monkey kidney cells. J. Bacteriol. 91:1295-1297. 1966.-Synthesis of the simian virus 40 (SV40) T antigen in primary African green monkey kidney cells was abolis...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.91.3.1295-1297.1966
更新日期:1966-03-01 00:00:00
abstract::X-ray fluorescence spectroscopy and electron microscopy of unstained specimens have been used to study the binding of chloropentaammineosmium(III) chloride to isolated walls of Bacillus subtilis. Native walls bound 0.220 mumol of the osmium probe per mg (dry weight) of walls, whereas walls which were chemically treate...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.3.1120-1123.1982
更新日期:1982-03-01 00:00:00
abstract::The diphtheria toxin repressor (DtxR) of Corynebacterium diphtheriae uses Fe(2+) as a corepressor. Holo-DtxR inhibits transcription from the iron-regulated promoters (IRPs) designated IRP1 through IRP5 as well as from the promoters for the tox and hmuO genes. DtxR binds to 19-bp operators with the consensus sequence 5...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.2.432-438.2000
更新日期:2000-01-01 00:00:00
abstract::We have used Tn917lacZ to mutagenize the Bacillus subtilis chromosome and have isolated mutants that are defective in chemotaxis and motility. Mapping of the transposon inserts identified two new loci. Mutations in one of these loci generated mutants that had paralyzed flagella. Accordingly, we designate this a mot lo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.12.6841-6848.1990
更新日期:1990-12-01 00:00:00