Mapping and disruption of the chpB locus in Escherichia coli.

abstract：:A Bacillus subtilis ribose transport operon (rbs) was shown to be subject to AbrB-mediated control through direct AbrB-DNA binding interactions in the vicinity of the promoter. Overproduction of AbrB was shown to relieve catabolite repression of rbs during growth in the presence of poorer carbon sources such as arabin...

journal_title：Journal of bacteriology

authors： Strauch MA

更新日期：1995-12-01 00:00:00

abstract：:The β-barrel assembly machinery (BAM) complex is the core machinery for the assembly of β-barrel membrane proteins, and inhibition of BAM complex activity is lethal to bacteria. Discovery of integral membrane proteins that are key to pathogenesis and yet do not require assistance from the BAM complex raises the questi...

journal_title：Journal of bacteriology

authors： Hay ID,Belousoff MJ,Dunstan RA,Bamert RS,Lithgow T

更新日期：2018-02-07 00:00:00

abstract：:Enterococcus faecalis ATCC 11700 is able to use arginine and the diamine agmatine as a sole energy source. Via the highly homologous deiminase pathways, arginine and agmatine are converted into CO2, NH3, and the end products ornithine and putrescine, respectively. In the arginine deiminase pathway, uptake of arginine ...

journal_title：Journal of bacteriology

authors： Driessen AJ,Smid EJ,Konings WN

更新日期：1988-10-01 00:00:00

abstract：:The cysteines of the Escherichia coli periplasmic enzyme alkaline phosphatase, which are involved in disulfide bonds in the native enzyme, were found to be fully reduced when the protein was retained in the cytoplasm. Under these circumstances the cysteines remained reduced for at least several minutes after the synth...

journal_title：Journal of bacteriology

authors： Derman AI,Beckwith J

更新日期：1991-12-01 00:00:00

abstract：:The arginine biosynthetic precursors, ornithine, citrulline, and argininosuccinate, inhibit arginyl-transfer ribonucleic acid (tRNA) synthetase (EC 6.1.1.13, arginine: soluble RNA ligase, adenosine monophosphate) activity in the in vitro attachment assay system. Ornithine is the most potent, argininosuccinate is next,...

journal_title：Journal of bacteriology

authors： Yem DW,Williams LS

更新日期：1971-08-01 00:00:00

abstract：:Escherichia coli threonyl-tRNA synthetase is a homodimeric protein that acts as both an enzyme and a regulator of gene expression: the protein aminoacylates tRNA(Thr) isoacceptors and binds to its own mRNA, inhibiting its translation. The enzyme contains a zinc atom in its active site, which is essential for the recog...

journal_title：Journal of bacteriology

authors： Caillet J,Graffe M,Eyermann F,Romby P,Springer M

更新日期：2007-10-01 00:00:00

abstract：:This study investigated the apparent genetic redundancy in the biosynthesis of polyhydroxyalkanoates (PHAs) in the Rhodospirillum rubrum genome revealed by the occurrence of three homologous PHA polymerase genes (phaC1, phaC2, and phaC3). In vitro biochemical assays established that each gene product encodes PHA polym...

journal_title：Journal of bacteriology

authors： Jin H,Nikolau BJ

更新日期：2012-10-01 00:00:00

abstract：:Ellner, Paul D. (University of Vermont, Burlington) and Carolyn D. Bohan. Serology of the soluble antigens of Clostridium perfringens types A-F by agar-gel diffusion. J. Bacteriol. 83:284-296. 1962.-A serological study by agar-gel diffusion of the soluble antigens of 39 strains of the Clostridium perfringens group has...

journal_title：Journal of bacteriology

authors： ELLNER PD,BOHAN CD

更新日期：1962-02-01 00:00:00

abstract：:Secreted and cell envelope-associated proteins are important to both Mycobacterium tuberculosis pathogenesis and the generation of protective immunity to M. tuberculosis. We used an in vitro Tn552'phoA transposition system to identify exported proteins of M. tuberculosis. The system is simple and efficient, and the tr...

journal_title：Journal of bacteriology

authors： Braunstein M,Griffin TJ IV,Kriakov JI,Friedman ST,Grindley ND,Jacobs WR Jr

更新日期：2000-05-01 00:00:00

abstract：:Cysteine and methionine availability influences many processes in the cell. In bacteria, transcription of the specific genes involved in the synthesis of these two amino acids is usually regulated by different mechanisms or regulators. Pathways for the synthesis of cysteine and methionine and their interconversion wer...

journal_title：Journal of bacteriology

authors： Sperandio B,Polard P,Ehrlich DS,Renault P,Guédon E

更新日期：2005-06-01 00:00:00

abstract：:Transcription of the trp operon of Bacillus subtilis is regulated by attenuation. A trpE'-'lacZ gene fusion preceded by the wild-type trp promoter-leader region was used to analyze regulation. Overproduction of the trp leader transcript in trans from a multicopy plasmid caused constitutive expression of the chromosoma...

journal_title：Journal of bacteriology

authors： Kuroda MI,Henner D,Yanofsky C

更新日期：1988-07-01 00:00:00

abstract：:We previously described a gene, lpt3, required for the addition of phosphoethanolamine (PEtn) at the 3 position on the beta-chain heptose (HepII) of the inner-core Neisseria meningitidis lipopolysaccharide (LPS), but it has long been recognized that the inner-core LPS of some strains possesses PEtn at the 6 position (...

journal_title：Journal of bacteriology

authors： Wright JC,Hood DW,Randle GA,Makepeace K,Cox AD,Li J,Chalmers R,Richards JC,Moxon ER

更新日期：2004-10-01 00:00:00

abstract：:Benzoyl coenzyme A (benzoyl-CoA) reductase is a key enzyme in the anaerobic metabolism of aromatic compounds catalyzing the ATP-driven reductive dearomatization of benzoyl-CoA. The enzyme from Thauera aromatica uses a reduced 2[4Fe-4S] ferredoxin as electron donor. In this work, we identified 2-oxoglutarate:ferredoxin...

journal_title：Journal of bacteriology

authors： Dörner E,Boll M

更新日期：2002-07-01 00:00:00

abstract：:Methanogenesis from the non-physiological C1 donors thioproline, thiazolidine, hexamethylenetetramine, formaldehyde (HCHO), and HOCH2-S-coenzyme M (CoM) was catalyzed by cell extracts of Methanobacterium thermoautotrophicum under a hydrogen atmosphere. Tetrahydromethanopterin (H4MPT) and HS-CoM were required in the re...

journal_title：Journal of bacteriology

authors： Escalante-Semerena JC,Wolfe RS

更新日期：1985-02-01 00:00:00

abstract：:Histidine decarboxylases from Klebsiella planticola and Enterobacter aerogenes were purified to homogeneity and compared with the histidine decarboxylase from Morganella morganii. All three enzymes required pyridoxal 5'-phosphate as a coenzyme, showed optimal activity at pH 6.5, decarboxylated only histidine among the...

journal_title：Journal of bacteriology

authors： Guirard BM,Snell EE

更新日期：1987-09-01 00:00:00

abstract：:Acidified phenol extracts prepared from competent cultures of a group H Streptococcus strain Wicky made competent with competence factor derived from cultures of another group H Streptococcus, strain Challis, showed a difference in polyacrylamide-gel protein patterns when compared to extracts prepared from noncompeten...

journal_title：Journal of bacteriology

authors： Ranhand JM,Theodore TS,Cole RM

更新日期：1970-10-01 00:00:00

abstract：:Mutants with altered forms of GltC, a positive LysR-type regulator of Bacillus subtilis glutamate synthase gene expression, were isolated. The mutant GltC proteins stimulated expression from the wild-type gltA promoter region 1.5- to 2.0-fold and from mutant promoter regions up to 80-fold. Moreover, expression of gltA...

journal_title：Journal of bacteriology

authors： Belitsky BR,Sonenshein AL

更新日期：1995-10-01 00:00:00

abstract：:Glutathione and soluble thiol content were examined in a broad spectrum of bacteria. Significant soluble thiol was present in all cases. The thiol compound was glutathione in most of the gram-negative bacteria but not in most of the gram-positive bacteria studied. Glutathione was absent in four anerobes and one microa...

journal_title：Journal of bacteriology

authors： Fahey RC,Brown WC,Adams WB,Worsham MB

更新日期：1978-03-01 00:00:00

abstract：:The fatty acid pattern in three hydrocarbon-utilizing bacteria during growth on various substrates was examined. The predominant fatty acids in acetate-grown cells were C(16), C(16:1), C(18:1), and Br-C(19) and the major fatty acids in propane-grown cells were C(15), C(17), C(17:1), C(18:1), and Br-C(18). When one org...

journal_title：Journal of bacteriology

authors： Dunlap KR,Perry JJ

更新日期：1967-12-01 00:00:00

abstract：:Starvation of Escherichia coli for potassium, phosphate, or magnesium ions leads to a reversible increase in the rate of protein degradation and an inhibition of ribonucleic acid (RNA) synthesis. In cells deprived of potassium, the breakdown of the more stable cell proteins increased two- to threefold, whereas the hyd...

journal_title：Journal of bacteriology

authors： St John AC,Goldberg AL

更新日期：1980-09-01 00:00:00

abstract：:The photoautotrophic cyanobacterium Anacystis nidulans was used to investigate the membrane transport of branched-chain, neutral amino acids and its dependence on photosynthetic reactions. The uptake of alpha-amino [1-14C]isobutyric acid and L-[1-14C]leucine followed Michaelis, Menten kinetics and resulted in an energ...

journal_title：Journal of bacteriology

authors： Lee-Kaden J,Simonis W

更新日期：1982-07-01 00:00:00

abstract：:Aeromonas hydrophila AH-3 lateral flagella are not assembled when bacteria grow in liquid media; however, lateral flagellar genes are transcribed. Our results indicate that A. hydrophila lateral flagellar genes are transcribed at three levels (class I to III genes) and share some similarities with, but have many impor...

journal_title：Journal of bacteriology

authors： Wilhelms M,Gonzalez V,Tomás JM,Merino S

更新日期：2013-04-01 00:00:00

abstract：:Streptococcus mutans transports glucose via the phosphoenolpyruvate (PEP)-dependent sugar phosphotransferase system (PTS). Earlier studies indicated that an alternate glucose transport system functions in this organism under conditions of high growth rates, low pH, or excess glucose. To identify this system, S. mutans...

journal_title：Journal of bacteriology

authors： Cvitkovitch DG,Boyd DA,Thevenot T,Hamilton IR

更新日期：1995-05-01 00:00:00

abstract：:The genetic organization of the polypeptides required for the biosynthesis of the F41 adhesin of enterotoxigenic Escherichia coli strains was investigated. Maxicell analysis demonstrated that a recombinant plasmid which mediated mannose-resistant hemagglutination and F41 antigen production encoded four polypeptides of...

journal_title：Journal of bacteriology

authors： Anderson DG,Moseley SL

更新日期：1988-10-01 00:00:00

abstract：:Renaturable regions in the DNA strands of the N group plasmid pCU1 have been visualized as stem-loop structures by electron microscopy. Four such distinct structures are described, the smallest of which is within the loop of a larger one. The region of pCU1 in which these structures occur has several restriction sites...

journal_title：Journal of bacteriology

authors： Konarska-Kozlowska M,Thatte V,Iyer VN

更新日期：1983-03-01 00:00:00

abstract：:We have identified IS100 sequences in a specific subset of Yersinia pseudotuberculosis isolates that were also sensitive to the Y. pestis-produced bacteriocin, pesticin. In contrast, Y. pseudotuberculosis strains which did not contain IS100 sequences were not sensitive to pesticin. We propose that IS100 serves as a mo...

journal_title：Journal of bacteriology

authors： McDonough KA,Hare JM

更新日期：1997-03-01 00:00:00

abstract：:Clostridium difficile is a gram-positive, spore-forming enteric anaerobe which can infect humans and a wide variety of animal species. Recently, the incidence and severity of human C. difficile infection has markedly increased. In this study, we evaluated the genomic content of 73 C. difficile strains isolated from hu...

journal_title：Journal of bacteriology

authors： Janvilisri T,Scaria J,Thompson AD,Nicholson A,Limbago BM,Arroyo LG,Songer JG,Gröhn YT,Chang YF

更新日期：2009-06-01 00:00:00

abstract：:We previously developed a genetic approach to study, with a single antibody, the topology of the outer membrane protein LamB, an Escherichia coli porin with specificity towards maltodextrins and a receptor for bacteriophage lambda. Our initial procedure consisted of inserting at random the same reporter epitope (the C...

journal_title：Journal of bacteriology

authors： Newton SM,Klebba PE,Michel V,Hofnung M,Charbit A

更新日期：1996-06-01 00:00:00

abstract：:The virA and virG gene products are required for the regulation of the vir regulon on the tumor-inducing (Ti) plasmid of Agrobacterium tumefaciens. VirA is a membrane-associated protein which is homologous to the sensor molecules of other two-component regulatory systems. We overproduced truncated VirA proteins in Esc...

journal_title：Journal of bacteriology

authors： Jin S,Roitsch T,Ankenbauer RG,Gordon MP,Nester EW

更新日期：1990-02-01 00:00:00

abstract：:The Salmonella typhimurium aroF gene, encoding the tyrosine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase, was localized to a chromosomal PstI fragment by Southern blotting with an Escherichia coli aroF probe. This fragment was cloned by screening a plasmid library for complementation of an E. ...

journal_title：Journal of bacteriology

authors： Muday GK,Herrmann KM

更新日期：1990-05-01 00:00:00