Abstract:
:Bacteriocins are ribosomally synthesized proteinaceous antibacterial peptides. They selectively interfere with the growth of other bacteria. The production and secretion of bacteriocins confer a distinct ecological advantage to the producer in competing against other bacteria that are present in the same ecological niche. Streptococcus mutans, a significant contributor to the development of dental caries, is one of the most prolific producers of bacteriocins, known as mutacins in S. mutans In this study, we characterized the locus encoding mutacin B-Ny266, a lantibiotic with a broad spectrum of activity. The chromosomal locus is composed of six predicted operon structures encoding proteins involved in regulation, antimicrobial activity, biosynthesis, modification, transport, and immunity. Mutacin B-Ny266 was purified from semisolid cultures, and two inhibitory peptides, LanA and LanA', were detected. Both peptides were highly modified. Such modifications include dehydration of serine and threonine and the formation of a C-terminal aminovinyl-cysteine (AviCys) ring. While LanA peptide alone is absolutely required for antimicrobial activity, the presence of LanA' enhanced the activity of LanA, suggesting that B-Ny266 may function as a two-peptide lantibiotic. The activation of lanAA' expression is most likely controlled by the conserved two-component system NsrRS, which is activated by LanA peptide but not by LanA'. The chromosomal locus encoding mutacin B-Ny266 was not universally conserved in all sequenced S. mutans genomes. Intriguingly, the genes encoding LanAA' peptides were restricted to the most invasive serotypes of S. mutansIMPORTANCE Although dental caries is largely preventable, it remains the most common and costly infectious disease worldwide. Caries is initiated by the presence of dental plaque biofilm that contains Streptococcus mutans, a species extensively characterized by its role in caries development and formation. S. mutans deploys an arsenal of strategies to establish itself within the oral cavity. One of them is the production of bacteriocins that confer a competitive advantage by targeting and killing closely related competitors. In this work, we found that mutacin B-Ny266 is a potent lantibiotic that is effective at killing a wide array of oral streptococci, including nearly all S. mutans strains tested. Lantibiotics produced by oral bacteria could represent a promising strategy to target caries pathogens embedded in dental plaque biofilm.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Dufour D,Barbour A,Chan Y,Cheng M,Rahman T,Thorburn M,Stewart C,Finer Y,Gong SG,Lévesque CMdoi
10.1128/JB.00762-19subject
Has Abstractpub_date
2020-05-27 00:00:00issue
12eissn
0021-9193issn
1098-5530pii
JB.00762-19journal_volume
202pub_type
杂志文章abstract::The nature of the phospholipids of the various bacteria that have ice nucleation activity in supercooled water has been determined. The seven bacteria studied included Pseudomonas syringae, Erwinia herbicola, three Escherichia coli K-12 strains that are phenotypically Ice+ because they contain plasmids with different ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.6.2053-2060.1991
更新日期:1991-03-01 00:00:00
abstract::In bacteria like Escherichia coli, the accumulation of glucose-6-phosphate (G6P) or its analogs such as α-methyl glucoside-6-phosphate (αMG6P) results in stress that appears in the form of growth inhibition. The small RNA SgrS is an essential part of the response that helps E. coli combat glucose-phosphate stress; the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00705-13
更新日期:2013-11-01 00:00:00
abstract::Legionella pneumophila expresses two catalase-peroxidase enzymes that exhibit strong peroxidatic but weak catalatic activities, suggesting that other enzymes participate in decomposition of hydrogen peroxide (H2O2). Comparative genomics revealed that L. pneumophila and its close relative Coxiella burnetii each contain...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00635-06
更新日期:2006-09-01 00:00:00
abstract::The alpha-aminoadipate pathway for lysine biosynthesis is present only in fungi. The alpha-aminoadipate reductase (AAR) of this pathway catalyzes the conversion of alpha-aminoadipic acid to alpha-aminoadipic-delta-semialdehyde by a complex mechanism involving two gene products, Lys2p and Lys5p. The LYS2 and LYS5 genes...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.183.24.7120-7125.2001
更新日期:2001-12-01 00:00:00
abstract::Methylation of DNA at the C-5 position of cytosine occurs in diverse organisms. This modification can increase the rate of C→T transitions at the methylated position. In Escherichia coli and related enteric bacteria, the inner C residues of the sequence CCWGG (W is A or T) are methylated by the Dcm enzyme. These sites...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00371-18
更新日期:2018-11-26 00:00:00
abstract::Horizontal acquisition of novel chromosomal genes is considered to be a key process in the evolution of bacterial pathogens. However, the identification of gene presence or absence could be hindered by the inconsistencies in bacterial genome annotations. Here, we performed a cross-annotation of omnipresent core and mo...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00545-16
更新日期:2016-11-04 00:00:00
abstract::A characteristic of N2-fixing cyanobacteria in symbiotic associations appears to be release of N2-derived NH4+. The specific activity of the primary ammonium-assimilating enzyme, glutamine synthetase (GS), was found to be three- to fourfold lower in Nostoc sp. strain 7801 grown in symbiotic association with the bryoph...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.6.2471-2475.1987
更新日期:1987-06-01 00:00:00
abstract::The degradation of synthetic compounds requires bacteria to recruit and adapt enzymes from pathways for naturally occurring compounds. Previous work defined the steps in 2,4-dinitrotoluene (2,4-DNT) metabolism through the ring fission reaction. The results presented here characterize subsequent steps in the pathway th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.15.4219-4232.2002
更新日期:2002-08-01 00:00:00
abstract::The SasG surface protein of Staphylococcus aureus has been shown to promote the formation of biofilm. SasG comprises an N-terminal A domain and repeated B domains. Here we demonstrate that SasG is involved in the accumulation phase of biofilm, a process that requires a physiological concentration of Zn(2+). The B doma...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00628-10
更新日期:2010-11-01 00:00:00
abstract::Three homofermentative (Lactobacillus plantarum B38, L. plantarum B33, Pediococcus pentosaceus B30) and three heterofermentative (Leuconostoc mesenteroides 39, L. oenos B70, Lactobacillus brevis) lactic acid bacteria were examined for the presence or absence of nicotinamide adenine dinucleotide (NAD)-dependent and NAD...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.108.3.1284-1289.1971
更新日期:1971-12-01 00:00:00
abstract::The translation inhibitor microcin C7 (MccC7) is a linear heptapeptide whose N terminus has been replaced by an N-formyl group and whose C terminus has been replaced by the phosphodiester of 5'-adenylic acid and n-aminopropanol (J. I. Guijarro, J. E. González-Pastor, F. Baleux, J. L. San Millán, M. A. Castilla, M. Ric...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.24.7131-7140.1995
更新日期:1995-12-01 00:00:00
abstract::Early log-phase cells of Mycoplasma gallisepticum A5969 were synchronized by holding in Eagle minimal essential medium (MEM) for 2 h. When transferred out of MEM into tryptose medium, the cells exhibited synchronous growth. Deoxyribonucleic acid (DNA) synthesis proceeded continuously during this growth but stopped dur...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.115.1.117-120.1973
更新日期:1973-07-01 00:00:00
abstract::At specific times in the cell cycle, the bacterium Caulobacter crescentus assembles two major polar organelles, the flagellum and the stalk. Previous studies have shown that flbT mutants overproduce flagellins and are unable to form chemotaxis swarm rings. In this paper, we report alterations in both the stalk and the...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.4.2113-2123.1990
更新日期:1990-04-01 00:00:00
abstract::sigma E is a sporulation-specific sigma factor of Bacillus subtilis that is formed from an inactive precursor protein (pro-sigma E) by the removal of 27 to 29 amino acids from the pro-sigma E amino terminus. By using oligonucleotide-directed mutagenesis, sequential deletions were constructed in the precursor-specific ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.174.14.4629-4637.1992
更新日期:1992-07-01 00:00:00
abstract::Among mutants which require isoleucine, but not valine, for growth, we have found two distinguishable classes. One is defective in the biosynthetic enzyme threonine deaminase (l-threonine hydro-lyase, deaminating, EC 4.2.1.16) and the other has an altered isoleucyl transfer ribonucleic acid (tRNA) synthetase [l-isoleu...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.105.2.527-537.1971
更新日期:1971-02-01 00:00:00
abstract::Particular combinations of fungal strains and transformation vectors allow for fungal rearrangement of normally integrative plasmids, resulting in the creation of linear self-replicating plasmids in Fusarium oxysporum. The rearrangement results in the addition of fungal DNA, including telomere consensus sequences, to ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.172.6.3163-3171.1990
更新日期:1990-06-01 00:00:00
abstract::Conti, S. F. (Dartmouth Medical School, Hanover, N.H.), and Peter Hirsch. Biology of budding bacteria. III. Fine structure of Rhodomicrobium and Hyphomicrobium spp. J. Bacteriol. 89:503-512. 1965.-The ultrastructure of 14 strains of hyphomicrobia, and of Rhodomicrobium vannielii, was investigated by means of electron ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.89.2.503-512.1965
更新日期:1965-02-01 00:00:00
abstract::Cells of Haemophilus influenzae strain Rd competent for genetic transformation irreversibly bound approximately five molecular fragments of H. influenzae deoxyribonucleic acid (DNA) per cell; under identical conditions, DNA derived from Escherichia coli B was not taken up (<1 molecule per 50 cells). Similarly, DNA fro...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.118.2.369-373.1974
更新日期:1974-05-01 00:00:00
abstract::Klebsiella pneumoniae can use nitrate and nitrite as sole nitrogen sources through the nitrate assimilatory pathway. The structural genes for assimilatory nitrate and nitrite reductases together with genes necessary for nitrate transport form an operon, nasFEDCBA. Expression of the nasF operon is regulated both by gen...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.176.16.5077-5085.1994
更新日期:1994-08-01 00:00:00
abstract::Alkaline phosphatase (APase) is induced as a culture enters stationary phase because of limiting phosphate. The results presented here show that expression of APase is regulated both negatively and positively. PhoP, a homolog of a family of bacterial transcription factors, and PhoR, a homolog of bacterial histidine pr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.175.12.3749-3756.1993
更新日期:1993-06-01 00:00:00
abstract::A clinical isolate of Staphylococcus aureus was found to be tolerant (MBC much greater than MIC) to a number of beta-lactam antibiotics, including oxacillin. Biophotometric analysis showed that a number of concentrations of oxacillin were capable of stimulating rapid cellular lysis in this organism, but the extent of ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.170.4.1831-1836.1988
更新日期:1988-04-01 00:00:00
abstract::Rubrerythrin was purified by multistep chromatography under anaerobic, reducing conditions from the hyperthermophilic archaeon Pyrococcus furiosus. It is a homodimer with a molecular mass of 39.2 kDa and contains 2.9 +/- 0.2 iron atoms per subunit. The purified protein had peroxidase activity at 85 degrees C using hyd...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.186.23.7888-7895.2004
更新日期:2004-12-01 00:00:00
abstract::We present the complete genome sequence of Yersinia pestis KIM, the etiologic agent of bubonic and pneumonic plague. The strain KIM, biovar Mediaevalis, is associated with the second pandemic, including the Black Death. The 4.6-Mb genome encodes 4,198 open reading frames (ORFs). The origin, terminus, and most genes en...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.184.16.4601-4611.2002
更新日期:2002-08-01 00:00:00
abstract::LaBrec, Eugene H., Herman Schneider, Thomas J. Magnani, and Samuel B. Formal. Epithelial cell penetration as an essential step in the pathogenesis of bacillary dysentery. J. Bacteriol. 88:1503-1518. 1964.-A parent strain of Shigella flexneri 2a and a colonial mutant derived from it were studied in three animal models....
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.88.5.1503-1518.1964
更新日期:1964-11-01 00:00:00
abstract::Yeast and hyphal walls of Candida albicans were extracted with sodium dodecyl sulfate (SDS). Some of the extracted proteins reacted with a specific beta-1,6-glucan antiserum but not with a beta-1,3-glucan antiserum. They lost their beta-1,6-glucan epitope after treatment with ice-cold aqueous hydrofluoric acid, sugges...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.13.3788-3792.1995
更新日期:1995-07-01 00:00:00
abstract::Freter, Rolf (Jefferson Medical College, Philadelphia, Pa.), and Atsushi Ozawa. Explanation for limitation of populations of Escherichia coli in broth cultures. J. Bacteriol. 86:904-910. 1963.-Veal Infusion Broth surrounding a cellophane bag containing a 24-hr culture of Escherichia coli was inhibitory to an inoculum ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.86.5.904-910.1963
更新日期:1963-11-01 00:00:00
abstract::The isolation of auxotrophic mutants of Bacillus licheniformis, a microbe containing constitutive beta-lactamase activity, was found to be facilitated by the addition of clavulanic acid and cefotaxime during enrichment. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.159.2.803-804.1984
更新日期:1984-08-01 00:00:00
abstract::Methanol dehydrogenase, the enzyme that oxidizes methanol to formaldehyde in gram-negative methylotrophs, contains the prosthetic group pyrroloquinoline quinone (PQQ). To begin to analyze how the synthesis of PQQ is coordinated with the production of other methanol dehydrogenase components, the transcription of one of...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.1.206-211.1995
更新日期:1995-01-01 00:00:00
abstract::Genome sequencing revealed that all six chlamydiae genomes contain three groEL-like genes (groEL1, groEL2, and groEL3). Phylogenetic analysis of groEL1, groEL2, and groEL3 indicates that these genes are likely to have been present in chlamydiae since the beginning of the lineage. Comparison of deduced amino acid seque...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.185.6.1958-1966.2003
更新日期:2003-03-01 00:00:00
abstract::Pathways of ammonia assimilation into glutamic acid in Bacillus macerans were investigated by measurements of the specific activities of glutamate dehydrogenase (GDH), glutamine synthetase, and glutamate synthase. In ammonia-rich medium, GDH was the predominant pathway of ammonia assimilation. In nitrogen-fixing cells...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.169.10.4692-4695.1987
更新日期:1987-10-01 00:00:00