Abstract:
:Three homofermentative (Lactobacillus plantarum B38, L. plantarum B33, Pediococcus pentosaceus B30) and three heterofermentative (Leuconostoc mesenteroides 39, L. oenos B70, Lactobacillus brevis) lactic acid bacteria were examined for the presence or absence of nicotinamide adenine dinucleotide (NAD)-dependent and NAD-independent d- and l-lactate dehydrogenases. Two of the six strains investigated, P. pentosaceus and L. oenos, did not exhibit an NAD-independent enzyme activity capable of reducing dichlorophenol indophenol. The pH optima of the lactic dehydrogenases were determined. The NAD-dependent enzymes from homofermentative strains exhibited optima at pH 7.8 to 8.8, whereas values from 9.0 to 10.0 were noted for these enzymes from heterofermentative organisms. The optima for the NAD-independent enzymes were between 5.8 and 6.6. The apparent Michaelis-Menten constants determined for both NAD and the substrates demonstrated the existence of a greater affinity for d- than l-lactic acid. A comparison of the specific NAD-dependent and NAD-independent lactate dehydrogenase activities revealed a direct correlation of the d/l ratios of these activities with the type of lactic acid produced during the growth of the organism.
journal_name
J Bacterioljournal_title
Journal of bacteriologyauthors
Doelle HWdoi
10.1128/JB.108.3.1284-1289.1971subject
Has Abstractpub_date
1971-12-01 00:00:00pages
1284-9issue
3eissn
0021-9193issn
1098-5530journal_volume
108pub_type
杂志文章abstract::Xylose is rarely described as a component of bacterial glycans. UDP-xylose is the nucleotide-activated form necessary for incorporation of xylose into glycans and is synthesized by the decarboxylation of UDP-glucuronic acid (UDP-GlcA). Enzymes with UDP-GlcA decarboxylase activity include those that lead to the formati...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.05337-11
更新日期:2011-10-01 00:00:00
abstract::Four chloramphenicol resistance (Cm) and four tetracycline resistance (Tc) plasmids from Staphylococcus aureus were characterized by restriction endonuclease mapping. All four Tc plasmids had molecular masses of 2.9 megadaltons (Mdaltons) and indistinguishable responses to seven different restriction endonucleases. Th...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.136.1.402-413.1978
更新日期:1978-10-01 00:00:00
abstract::Ribonucleotide reductases (RNRs) are crucial to all living cells, since they provide deoxyribonucleotides (dNTPs) for DNA synthesis and repair. In Mycobacterium tuberculosis, a class Ib RNR comprising nrdE- and nrdF2-encoded subunits is essential for growth in vitro. Interestingly, the genome of this obligate human pa...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01409-08
更新日期:2009-02-01 00:00:00
abstract::The ligated loop of the rabbit intestine was investigated as a possible experimental model for the study of Clostridium perfringens food poisoning. The method of preparation of the challenge inoculum was important in determining whether a given strain would provoke a response. When cultures were grown for 4 hr at 37 C...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.95.5.1560-1566.1968
更新日期:1968-05-01 00:00:00
abstract::In this study, we demonstrated the remarkable genome plasticity of lytic lactococcal phages that allows them to rapidly adapt to the dynamic dairy environment. The lytic double-stranded DNA phage ul36 was used to sequentially infect a wild-type strain of Lactococcus lactis and two isogenic derivatives with genes encod...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01111-06
更新日期:2007-02-01 00:00:00
abstract::The flagellar regulon of Brucella melitensis 16M contains 31 genes clustered in three loci on the small chromosome. These genes encode a polar sheathed flagellum that is transiently expressed during vegetative growth and required for persistent infection in a mouse model. By following the expression of three flagellar...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00712-06
更新日期:2007-01-01 00:00:00
abstract::We have previously shown that both ends of the Tn3 family transposon Tn4652 contain integration host factor (IHF) binding sites and that IHF positively regulates expression of the Tn4652 transposase gene tnpA in Pseudomonas putida (R. Hõrak, and M. Kivisaar, J. Bacteriol. 180:2822-2829, 1998). Tn4652 can activate sile...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.182.3.589-598.2000
更新日期:2000-02-01 00:00:00
abstract::Lipoprotein anchoring in bacteria is mediated by the prolipoprotein diacylglyceryl transferase (Lgt), which catalyzes the transfer of a diacylglyceryl moiety to the prospective N-terminal cysteine of the mature lipoprotein. Deletion of the lgt gene in the gram-positive pathogen Listeria monocytogenes (i) impairs intra...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00976-06
更新日期:2007-01-01 00:00:00
abstract::The synthesis of cytosine deaminase in Salmonella typhimurium is repressed by pyrimidines. This repression is mediated by both a uridine and a cytidine compound, indicating a distinct difference in the regulation of synthesis of cytosine deaminase from the regulation of the de novo pyrimidine pathway enzymes. A salvag...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.149.3.1171-1174.1982
更新日期:1982-03-01 00:00:00
abstract::Several phosphate-starvation-inducible (psi) genetic loci in Salmonella typhimurium were identified by fusing the lacZ gene to psi promoters by using the Mu d1 and Mu d1-8 bacteriophages. Although several different starvation conditions were examined, the psi loci responded solely to phosphate deprivation. A regulator...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.166.2.666-669.1986
更新日期:1986-05-01 00:00:00
abstract::Flagellar motility is an important determinant of Campylobacter jejuni that is required for promoting interactions with various hosts to promote gastroenteritis in humans or commensal colonization of many animals. In a previous study, we identified a nonmotile mutant of C. jejuni 81-176 with a transposon insertion in ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01199-06
更新日期:2007-01-01 00:00:00
abstract::Toluene dioxygenase from Pseudomonas putida F1 has been studied extensively with aromatic substrates. The present work examined the toluene dioxygenase-catalyzed oxidation of various halogenated ethenes, propenes, butenes and nonhalogenated cis-2-pentene, an isomeric mix of 2-hexenes, cis-2-heptene, and cis-2-octene a...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.179.12.3858-3865.1997
更新日期:1997-06-01 00:00:00
abstract::There are significant differences in the large subunits of form I and form II ribulose 1,5-bisphosphate carboxylase/oxygenase isolated from Rhodopseudomonas sphaeroides. Two-dimensional peptide mapping of carboxymethylated large subunits clearly indicates that there are differences in the primary structure of the two ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.3.1188-1193.1985
更新日期:1985-12-01 00:00:00
abstract::The predicted amino acid sequence of Bacillus subtilis ycsK exhibits similarity to the GDSL family of lipolytic enzymes. Northern blot analysis showed that ycsK mRNA was first detected from 4 h after the onset of sporulation and that transcription of ycsK was dependent on SigK and GerE. The fluorescence of the YcsK-gr...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01527-06
更新日期:2007-03-01 00:00:00
abstract::The Rickettsia prowazekii sigma factor was overexpressed, purified, and used to reconstitute RNA polymerase holoenzyme species. R. prowazekii RNA polymerase-promoter complexes were unstable and remained dissociable and heparin sensitive under conditions in which the corresponding Escherichia coli complexes were not. T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.177.21.6301-6303.1995
更新日期:1995-11-01 00:00:00
abstract::Garrett et al. (Mol. Gen. Genet. 182:326-331, 1981) constructed strains of Escherichia coli harboring derivatives of plasmid pBR322 that carry the lysis genes (S, R, and Rz) of phage lambda. The plasmid construction placed the genes under control of the lactose operon operator-promotor (and thus of lac repressor). Ind...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.158.1.354-356.1984
更新日期:1984-04-01 00:00:00
abstract::The DNA-dependent RNA polymerase (EC 2.7.7.6) from Clostridium acetobutylicum DSM 1731 has been purified to homogeneity and characterized. The purified enzyme was composed of four subunits and had a molecular mass of 370,000 Da. Western immunoblot analysis with polyclonal antibodies against the sigma 70 subunit of Esc...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.6.2120-2124.1991
更新日期:1991-03-01 00:00:00
abstract::Growth of Escherichia coli B in simple media enriched with Mn(II) resulted in the elevation of the manganese-containing superoxide dismutase, whereas growth in such medium enriched with iron caused increased content of the iron-containing superoxide dismutase. Enrichment of the medium with Co(II), Cu(II), Mo(VI), Zn(I...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.160.1.137-142.1984
更新日期:1984-10-01 00:00:00
abstract::Extraction with pentane virtually abolished reduced nicotinamide adenine dinucleotide oxidase activity in small particles from Azotobacter vinelandii, but activity was largely restored by added ubiquinone. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.98.1.311-313.1969
更新日期:1969-04-01 00:00:00
abstract::Beta toxin is a neutral sphingomyelinase secreted by certain strains of Staphylococcus aureus. This virulence factor lyses erythrocytes in order to evade the host immune system as well as scavenge nutrients. The structure of beta toxin was determined at 2.4-A resolution using crystals that were merohedrally twinned. T...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00741-07
更新日期:2007-12-01 00:00:00
abstract::The phs mutation, which causes a pleiotropic growth defect, has been mapped and shown to be an allele of rpoA, the gene for the alpha subunit of RNA polymerase. The mutation is shown to cause a transcription defect in the arabinose operon, araBAD. ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.164.2.972-975.1985
更新日期:1985-11-01 00:00:00
abstract::Wilson, Dwight E. (Rensselaer Polytechnic Institute, Troy, N.Y.). Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose. J. Bacteriol. 84:295-301. 1962.-The L. Kansas and NK strains of Newcastle disease virus were chromatographed on diethylaminoethyl (DEAE) cellulose ion-exchange co...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.84.2.295-301.1962
更新日期:1962-08-01 00:00:00
abstract::Broad-host-range plasmids and cloning vectors were conjugatively transferred to Acetobacter xylinum. One of the plasmids, RP4::Mu cts61, was used for the insertion of Tn1 into the 16-, 44-, and 64-kilobase-pair plasmids of A. xylinum. The Tn1-labeled plasmids could be mobilized by a helper plasmid. Many of the Tn1 ins...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.165.1.336-339.1986
更新日期:1986-01-01 00:00:00
abstract::The O antigen is the outer part of the lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria and contains many repeats of an oligosaccharide unit. It contributes to antigenic variability and is essential to the full function and virulence of bacteria. Shigella is a Gram-negative human pathogen that ...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00737-10
更新日期:2011-01-01 00:00:00
abstract::Streptococcus pneumoniae is the causative agent of multiple diseases, including otitis media, pneumonia, bacteremia, and meningitis. Pneumolysin (Ply), a member of the cholesterol-dependent cytolytic pore-forming toxins, is produced by virtually all clinical isolates of S. pneumoniae, and strains in which the Ply gene...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.01489-08
更新日期:2009-04-01 00:00:00
abstract::The initiation process of deoxyribonucleic acid (DNA) replication in Escherichia coli has been studied using the thermoreversible dna initiation mutant E. coli HfrHl65/120/6 dna-252. This dna mutation was incorrectly classed as a dnaA mutation. Biochemical and genetic evidence suggests that the dna-252 mutant is a nov...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.3.1476-1486.1977
更新日期:1977-03-01 00:00:00
abstract::The cheF gene, which is involved in chemotaxis in Bacillus subtilis, has been cloned, expressed, and sequenced. This gene is contained in a 0.7-kilobase PstI DNA fragment that was isolated from a lambda Charon 4A B. subtilis chromosomal DNA library. This fragment was subcloned into the expression vector pSI-1 and show...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.171.3.1631-1637.1989
更新日期:1989-03-01 00:00:00
abstract::Cellular pigmentation is an important virulence factor of the oral pathogen Porphyromonas gingivalis Pigmentation has been associated with many bacterial functions, including but not limited to colonization, maintaining a local anaerobic environment by binding oxygen molecules, and defense against reactive oxygen spec...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.00832-16
更新日期:2017-06-27 00:00:00
abstract::The role of the energy status of the yeast cell in the sensitivity of cultures to two yeast toxins was examined by using 12K release from cells as a measure of toxin action. The Saccharomyces cerevisiae killer toxin bound to sensitive cells in the presence of drugs that interfered with the generation or use of energy,...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/JB.129.2.668-677.1977
更新日期:1977-02-01 00:00:00
abstract::The terminal oxidase content of Bacillus firmus OF4, a facultative alkaliphile that grows well over the pH range of 7.5 to 10.5, was studied by difference spectroscopy. Evidence was found for three terminal oxidases under different growth conditions. The growth pH and the stage of growth profoundly affected the expres...
journal_title:Journal of bacteriology
pub_type: 杂志文章
doi:10.1128/jb.173.16.5010-5016.1991
更新日期:1991-08-01 00:00:00