Hybrid pathway for chlorobenzoate metabolism in Pseudomonas sp. B13 derivatives.

Abstract:

:Derivatives of Pseudomonas sp. B13 which had acquired the capability to utilize 4-chloro- and 3,5-dichlorobenzoate as a consequence of the introduction of genes of the TOL plasmid of Pseudomonas putida mt-2 were studied. The utilization of these substrates, a property not shared by the parent strains, was shown to depend upon the combined activities of enzymes from the donor and from the recipient. During growth on 3-chloro-, 4-chloro-, and 3,5-dichlorobenzoate, predominantly the toluate 1,2-deoxygenase and both dihydrodihydroxybenzoate dehydrogenases of the parent strains were induced. On the other hand, no catechol 2,3-dioxygenase from P. putida mt-2 was detectable, so that degradation of chlorocatechols by the nonproductive meta-cleavage pathway was avoided. Instead of that, chlorocatechols were subject to ortho cleavage and totally degraded by the preexisting enzymes of Pseudomonas sp. B13.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Reineke W,Knackmuss HJ

doi

10.1128/JB.142.2.467-473.1980

subject

Has Abstract

pub_date

1980-05-01 00:00:00

pages

467-73

issue

2

eissn

0021-9193

issn

1098-5530

journal_volume

142

pub_type

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