Detection of XerC and XerD recombinases in gram-negative bacteria of the family Enterobacteriaceae.

Abstract:

:XerC and XerD are site-specific recombinases of the lambda integrase family which resolve multimeric replicons to monomers by acting at specific sites such as cer, ckr, nmr, parB, and psi, which are found in plasmids, or at the dif site found in the Escherichia coli chromosome. By using Southern hybridizations to cloned E. coli xerC and xerD genes and a cer-nmr plasmid-based resolution assay, the presence of these genes in several species of Enterobacteriaceae is shown.

journal_name

J Bacteriol

journal_title

Journal of bacteriology

authors

Sirois S,Szatmari G

doi

10.1128/jb.177.14.4183-4186.1995

subject

Has Abstract

pub_date

1995-07-01 00:00:00

pages

4183-6

issue

14

eissn

0021-9193

issn

1098-5530

journal_volume

177

pub_type

杂志文章
  • Induction of superoxide dismutases in Escherichia coli by manganese and iron.

    abstract::Growth of Escherichia coli B in simple media enriched with Mn(II) resulted in the elevation of the manganese-containing superoxide dismutase, whereas growth in such medium enriched with iron caused increased content of the iron-containing superoxide dismutase. Enrichment of the medium with Co(II), Cu(II), Mo(VI), Zn(I...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.160.1.137-142.1984

    authors: Pugh SY,DiGuiseppi JL,Fridovich I

    更新日期:1984-10-01 00:00:00

  • Chemoreceptors and flagellar motors are subterminally located in close proximity at the two cell poles in spirochetes.

    abstract::Green fluorescent protein (GFP) fusions, immunofluorescence microscopy, and cryo-electron tomography revealed that the chemoreceptors of the Lyme disease spirochete Borrelia burgdorferi form long, thin arrays near both cell poles. These arrays are in close proximity to the flagellar motors. This information provides a...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.01530-10

    authors: Xu H,Raddi G,Liu J,Charon NW,Li C

    更新日期:2011-05-01 00:00:00

  • Quantifying aggregation dynamics during Myxococcus xanthus development.

    abstract::Under starvation conditions, a swarm of Myxococcus xanthus cells will undergo development, a multicellular process culminating in the formation of many aggregates called fruiting bodies, each of which contains up to 100,000 spores. The mechanics of symmetry breaking and the self-organization of cells into fruiting bod...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.05188-11

    authors: Zhang H,Angus S,Tran M,Xie C,Igoshin OA,Welch RD

    更新日期:2011-10-01 00:00:00

  • Listeria monocytogenes relA and hpt mutants are impaired in surface-attached growth and virulence.

    abstract::We describe here the identification and characterization of two Listeria monocytogenes (Tn917-LTV3) relA and hpt transposon insertion mutants that were impaired in growth after attachment to a model surface. Both mutants were unable to accumulate (p)ppGpp in response to amino acid starvation, whereas the wild-type str...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.3.621-628.2002

    authors: Taylor CM,Beresford M,Epton HA,Sigee DC,Shama G,Andrew PW,Roberts IS

    更新日期:2002-02-01 00:00:00

  • Role of staphylococcal phage and SaPI integrase in intra- and interspecies SaPI transfer.

    abstract::SaPIbov2 is a member of the SaPI family of staphylococcal pathogenicity islands and is very closely related to SaPIbov1. Typically, certain temperate phages can induce excision and replication of one or more of these islands and can package them into special small phage-like particles commensurate with their genome si...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00619-07

    authors: Maiques E,Ubeda C,Tormo MA,Ferrer MD,Lasa I,Novick RP,Penadés JR

    更新日期:2007-08-01 00:00:00

  • Serine protease EpiP from Staphylococcus epidermidis catalyzes the processing of the epidermin precursor peptide.

    abstract::The function of serine protease EpiP in epidermin biosynthesis was investigated. Epidermin is synthesized as a 52-amino-acid precursor peptide, EpiA, which is posttranslationally modified and processed to the mature 22-amino-acid peptide antibiotic. epiP was expressed in Staphylococcus carnosus with xylose-regulated e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.178.1.284-288.1996

    authors: Geissler S,Götz F,Kupke T

    更新日期:1996-01-01 00:00:00

  • Genome sequence of a dominant, multidrug-resistant Acinetobacter baumannii strain, TCDC-AB0715.

    abstract::Acinetobacter baumannii has emerged as a significant nosocomial pathogen worldwide. The increasing trend of carbapenem and fluoroquinolone resistance in A. baumannii severely limits the usage of therapeutic antimicrobial agents. Here we report the genome sequence of a multidrug-resistant A. baumannii strain, TCDC-AB07...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00244-11

    authors: Chen CC,Lin YC,Sheng WH,Chen YC,Chang SC,Hsia KC,Liao MH,Li SY

    更新日期:2011-05-01 00:00:00

  • YqfS from Bacillus subtilis is a spore protein and a new functional member of the type IV apurinic/apyrimidinic-endonuclease family.

    abstract::The enzymatic properties and the physiological function of the type IV apurinic/apyrimidinic (AP)-endonuclease homolog of Bacillus subtilis, encoded by yqfS, a gene specifically expressed in spores, were studied here. To this end, a recombinant YqfS protein containing an N-terminal His6 tag was synthesized in Escheric...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.18.5380-5390.2003

    authors: Salas-Pacheco JM,Urtiz-Estrada N,Martínez-Cadena G,Yasbin RE,Pedraza-Reyes M

    更新日期:2003-09-01 00:00:00

  • Bacillus subtilis phosphorylated PhoP: direct activation of the E(sigma)A- and repression of the E(sigma)E-responsive phoB-PS+V promoters during pho response.

    abstract::The phoB gene of Bacillus subtilis encodes an alkaline phosphatase (PhoB, formerly alkaline phosphatase III) that is expressed from separate promoters during phosphate deprivation in a PhoP-PhoR-dependent manner and at stage two of sporulation under phosphate-sufficient conditions independent of PhoP-PhoR. Isogenic st...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.187.15.5166-5178.2005

    authors: Abdel-Fattah WR,Chen Y,Eldakak A,Hulett FM

    更新日期:2005-08-01 00:00:00

  • Identification of long intergenic repeat sequences associated with DNA methylation sites in Caulobacter crescentus and other alpha-proteobacteria.

    abstract::A systematic search for motifs associated with CcrM DNA methylation sites revealed four long (>100-bp) motifs (CIR sequences) present in up to 21 copies in Caulobacter crescentus. The CIR1 and CIR2 motifs exhibit a conserved inverted repeat organization, with a CcrM site in the center of one of the repeats. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.16.4997-5002.2003

    authors: Chen SL,Shapiro L

    更新日期:2003-08-01 00:00:00

  • Shuttle mutagenesis of Neisseria gonorrhoeae: pilin null mutations lower DNA transformation competence.

    abstract::The method of shuttle mutagenesis has been extended to Neisseria gonorrhoeae. We have constructed a defective mini-Tn3 derivative that encodes chloramphenicol resistance in both N. gonorrhoeae and Escherichia coli and selected for mutations in the chloramphenicol resistance gene that express higher levels of antibioti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.1.40-46.1990

    authors: Seifert HS,Ajioka RS,Paruchuri D,Heffron F,So M

    更新日期:1990-01-01 00:00:00

  • Cloning and characterization of the gene for a protein thiol-disulfide oxidoreductase in Bacillus brevis.

    abstract::The gene (bdb) for protein thiol-disulfide oxidoreductase cloned from Bacillus brevis was found to encode a polypeptide consisting of 117 amino acid residues with a signal peptide of 27 residues. Bdb contains a well-conserved motif, Cys-X-X-Cys, which functions as the active center of disulfide oxidoreductases such as...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.177.3.745-749.1995

    authors: Ishihara T,Tomita H,Hasegawa Y,Tsukagoshi N,Yamagata H,Udaka S

    更新日期:1995-02-01 00:00:00

  • Polymer-Producing Species of Arthrobacter.

    abstract::Gasdorf, Helen J. (Northern Regional Research Laboratory, Peoria, Ill.), R. G. Benedict, M. C. Cadmus, R. F. Anderson, and R. W. Jackson. Polymer-producing species of Arthrobacter. J. Bacteriol. 90:147-150.1965.-Two slime-producing microorganisms, designated as NRRL B-1973 and NRRL B-1797, were isolated from a Guatema...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.90.1.147-150.1965

    authors: Gasdorf HJ,Benedict RG,Cadmus MC,Anderson RF,Jackson RW

    更新日期:1965-07-01 00:00:00

  • Escherichia coli-Salmonella typhimurium hybrid nusA genes: identification of a short motif required for action of the lambda N transcription antitermination protein.

    abstract::The Escherichia coli nusA gene, nusAEc, encodes an essential protein that influences transcription elongation. Derivatives of E. coli in which the Salmonella typhimurium nusA gene, nusASt, has replaced nusAEc are viable. Thus, NusASt can substitute for NusAEc in supporting essential bacterial activities. However, hybr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.5.1394-1404.1994

    authors: Craven MG,Granston AE,Schauer AT,Zheng C,Gray TA,Friedman DI

    更新日期:1994-03-01 00:00:00

  • Chromosomal integration, tandem amplification, and deamplification in Pseudomonas putida F1 of a 105-kilobase genetic element containing the chlorocatechol degradative genes from Pseudomonas sp. Strain B13.

    abstract::Analysis of chlorobenzene-degrading transconjugants of Pseudomonas putida F1 which had acquired the genes for chlorocatechol degradation (clc) from Pseudomonas sp. strain B13 revealed that the clc gene cluster was present on a 105-kb amplifiable genetic element (named the clc element). In one such transconjugant, P. p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.17.4360-4369.1998

    authors: Ravatn R,Studer S,Springael D,Zehnder AJ,van der Meer JR

    更新日期:1998-09-01 00:00:00

  • Molecular characterization of the Lactococcus lactis LlaKR2I restriction-modification system and effect of an IS982 element positioned between the restriction and modification genes.

    abstract::The nucleotide sequence of the plasmid-encoded LlaKR2I restriction-modification (R-M) system of Lactococcus lactis subsp. lactis biovar diacetylactis KR2 was determined. This R-M system comprises divergently transcribed endonuclease (llaKR2IR) and methyltransferase (llaKR2IM) genes; located in the intergenic region is...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.180.22.5844-5854.1998

    authors: Twomey DP,McKay LL,O'Sullivan DJ

    更新日期:1998-11-01 00:00:00

  • Ribonucleic acid synthesis in T2-infected Escherichia coli during "stringent" control.

    abstract::The leucine auxotroph Escherichia coli 2961 exhibited stringent control of net ribonucleic acid (RNA) synthesis during amino acid starvation. After leucine was exhausted from the medium, the rate of uracil incorporation into RNA rapidly decreased to 2 to 4% of the prestarvation value. Infection of the starved cells wi...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.96.1.111-116.1968

    authors: Sharp K,Green MH

    更新日期:1968-07-01 00:00:00

  • Stable isotope peptide mass spectrometry to decipher amino acid metabolism in Dehalococcoides strain CBDB1.

    abstract::Dehalococcoides species are key players in the anaerobic transformation of halogenated solvents at contaminated sites. Here, we analyze isotopologue distributions in amino acid pools from peptides of Dehalococcoides strain CBDB1 after incubation with (13)C-labeled acetate or bicarbonate as a carbon source. The resulti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00049-12

    authors: Marco-Urrea E,Seifert J,von Bergen M,Adrian L

    更新日期:2012-08-01 00:00:00

  • Properties of pili from Escherichia coli SS142 that mediate mannose-resistant adhesion to mammalian cells.

    abstract::We isolated pili from Escherichia coli SS142. These pili had a diameter of 6 nm and an average length of 400 nm. They were composed of subunits with a molecular weight of 18,000. Their amino acid composition was determined; methionine and proline were not detected. The isolated pili retained mannose-resistant hemagglu...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.153.2.1038-1044.1983

    authors: Mett H,Kloetzlen L,Vosbeck K

    更新日期:1983-02-01 00:00:00

  • Purification and preliminary characterization of the Escherichia coli K-12 recF protein.

    abstract::The recF gene of Escherichia coli is known to encode an Mr-40,000 protein that is involved in DNA recombinationa nd postreplication DNA repair. To characterize the role of the recF gene product in these processes, the recF gene was cloned downstream of a tac promoter to facilitate overproduction of the recF gene produ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.172.11.6291-6299.1990

    authors: Griffin TJ 4th,Kolodner RD

    更新日期:1990-11-01 00:00:00

  • pIIICTX, a predicted CTXphi minor coat protein, can expand the host range of coliphage fd to include Vibrio cholerae.

    abstract::CTXphi is a filamentous bacteriophage that encodes cholera toxin. CTXphi infection of its host bacterium, Vibrio cholerae, requires the toxin-coregulated pilus (TCP) and the products of the V. cholerae tolQRA genes. Here, we have explored the role of OrfU, a predicted CTXphi minor coat protein, in CTXphi infection. Pr...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.185.3.1037-1044.2003

    authors: Heilpern AJ,Waldor MK

    更新日期:2003-02-01 00:00:00

  • Effect of pH on the proportions of polar lipids, in chemostat cultures of Bacillus subtilis.

    abstract::Significant changes in the relative proportions of the individual polar lipids of two strains of Bacillus subtilis were observed when the pH of their chemostat cultures was varied. In phosphate- and magnesium-limited cultures of B. subtilis var. niger NCIB 8058. lysylphosphatidylglycerol was present in higher proporti...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.120.3.999-1003.1974

    authors: Minnikin DE,Abdolrahimzadeh H

    更新日期:1974-12-01 00:00:00

  • Characterization of a CO: heterodisulfide oxidoreductase system from acetate-grown Methanosarcina thermophila.

    abstract::During the methanogenic fermentation of acetate by Methanosarcina thermophila, the CO dehydrogenase complex cleaves acetyl coenzyme A and oxidizes the carbonyl group (or CO) to CO2, followed by electron transfer to coenzyme M (CoM)-S-S-coenzyme B (CoB) and reduction of this heterodisulfide to HS-CoM and HS-CoB (A. P. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.176.22.6974-6979.1994

    authors: Peer CW,Painter MH,Rasche ME,Ferry JG

    更新日期:1994-11-01 00:00:00

  • Linker mutagenesis of the Caulobacter crescentus S-layer protein: toward a definition of an N-terminal anchoring region and a C-terminal secretion signal and the potential for heterologous protein secretion.

    abstract::Linker insertion mutagenesis was used to modify the paracrystalline surface layer (S-layer) protein (RsaA) of the gram-negative bacterium Caulobacter crescentus. Eleven unique BamHI linker insertions in the cloned rsaA gene were identified; at the protein level, these linker insertions introduced 4 to 6 amino acids at...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.179.3.601-611.1997

    authors: Bingle WH,Nomellini JF,Smit J

    更新日期:1997-02-01 00:00:00

  • Thermal adaptation in yeast: obligate psychrophiles are obligate aerobes, and obligate thermophiles are facultative anaerobes.

    abstract::The obligate psychrophilic yeasts Torulopsis psychrophila, T. austromarina, Leucosporidium frigidum, L. gelidum, and L. nivalis were obligate aerobes and were unable to grow anaerobically. In contrast, the obligate thermophilic yeasts T. bovina, T. pintolopesii, Candida slooffii, and Saccharomyces telluris were facult...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.136.2.815-817.1978

    authors: Watson K,Arthur H,Morton H

    更新日期:1978-11-01 00:00:00

  • Deoxyribonucleic acid sequence organization of a yeast plasmid.

    abstract::Two-micrometer deoxyribonucleic acid (DNA), a circular plasmid of Saccharomyces cerevisiae, contains two nontandem repeated sequences which are inverted with respect to one another. These repeated sequences together account for 21% of the molecule length. Restriction endonuclease analysis and electron microscopy demon...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.129.1.472-481.1977

    authors: Livingston DM,Klein HL

    更新日期:1977-01-01 00:00:00

  • Isolation and preliminary characterization of bacteriophage phi-mu-4.

    abstract::Shafia, Fred (University of Nebraska, Lincoln), and T. L. Thompson. Isolation and preliminary characterization of bacteriophage phimu-4. J. Bacteriol. 87:999-1002. 1964.-Bacteriophage phimu-4 was isolated from lysogenic Bacillus stearothermophilus NU strain 4, and was propagated in strain 10 of the same species. The p...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.87.5.999-1002.1964

    authors: Shafia F,Thompson TL

    更新日期:1964-05-01 00:00:00

  • Genome sequence of Lactobacillus fructivorans KCTC 3543.

    abstract::Lactobacillus fructivorans is important in the generation of particular flavors and in other ripening processes associated with fermented food. Here, we present the draft genome sequence of the type strain Lactobacillus fructivorans KCTC 3543 (1,373,326 bp, with a G+C content of 38.9%), which consists of 5 scaffolds. ...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/JB.00075-12

    authors: Nam SH,Choi SH,Kang A,Lee KS,Kim DW,Kim RN,Kim DS,Park HS

    更新日期:2012-04-01 00:00:00

  • A cryptic promoter in the O(R) region of bacteriophage lambda.

    abstract::A cryptic promoter, designated P alpha, initiates transcription within the O(R) region of bacteriophage lambda. Transcription from P alpha proceeds in the direction of the cI repressor gene from sites 46 and 48 bp preceding the PRM transcription start site. P alpha is likely to compete with both PR and PRM for formati...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.175.17.5648-5654.1993

    authors: Woody ST,Fong RS,Gussin GN

    更新日期:1993-09-01 00:00:00

  • Analysis of dofA, a fruA-dependent developmental gene, and its homologue, dofB, in Myxococcus xanthus.

    abstract::The developmentally regulated gene dofA, identified from pulse-labeling experiments by two-dimensional gel electrophoresis, and its homologue, dofB, were cloned and characterized in Myxococcus xanthus. Deletion of dofA and dofB did not affect the vegetative growth and development of M. xanthus. dofA was specifically e...

    journal_title:Journal of bacteriology

    pub_type: 杂志文章

    doi:10.1128/jb.184.24.6803-6810.2002

    authors: Horiuchi T,Akiyama T,Inouye S,Komano T

    更新日期:2002-12-01 00:00:00